FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Ji Wang,Huihui Li,Changbin Li,Yifeng Ding,Yuanshang Wang,Wenjuan Zhu,Jia Wang,Yanchun Shao,Hui Pan,Xiaohong Wang
Recently, using bacteriophages as new molecular probes in reliable platforms for the detection of bacterial pathogens has attracted more and more increasing attentions. In this paper, a novel isolated Myoviridae bacteriophage SEP37 was covalently immobilized onto gold nanoparticles (AuNPs) modified gold disk electrode (GDE) surfaces using cysteamine (Cys) as a crosslinker. Substrates of GDE-AuNPs-Cys-Phage SEP37 and specific capture of Salmonella cells had been characterized using scanning electron microscopy (SEM) separately. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to study the electrochemical response of the biosensor interface manufacturing and bacterial capture process. Under the optimal experimental conditions, this phage-based EIS biosensor was able to detect Salmonella with a wide linear range from 2 × 10 1 to 2 × 10 6 colony forming unit (CFU)/mL within 30 min in spiked lake water and lettuce samples, with a limit of detection (LOD) of 17 CFU/mL. The detection linear range of spiked chicken samples was 2 × 10 2 to 2 × 10 5 CFU/mL, with a LOD of 1.3 × 10 2 CFU/mL. In combination with a pre-enrichment process for 3.5 h, this assay could reach a LOD of 1 CFU/mL in chicken breast meat samples. Besides, this phage-based EIS biosensor provided good reproducibility and stability. This phage-based EIS biosensor opens a new opportunity for the detection of pathogenic bacteria using the inherent selectivity of bacteriophage recognition.
研究领域:
微生物学
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食品安全
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
Journal of Energy Chemistry
·
2024
·
IF 14
Xiaodong Bai,Chaoliang Zheng,Heng Zhang,Jian Liu,Panpan Wang,Baojia Xia,Jianling Li
PEO-based all-solid-state electrolytes are extensively utilized and researched owing to their exceptional safety, low-mass-density, and cost-effectiveness. However, the low oxidation potential of PEO makes the interface problem with the high-voltage cathode extremely severe. In this work, the impedance of PEO-based all-solid-state batteries with high-voltage cathode (NCM811) was studied at different potentials. The Nyquist plots displayed a gyrate arc at low-frequencies for NCM811/PEO interface. Based on the kinetic modeling, it was deduced that there is a decomposition reaction of PEO-matrix in addition to de-embedded reaction of NCM811, and the PEO intermediate product (dehydra-PEO) adsorbed on the electrode surface leading to low-frequency inductive arcs. Furthermore, the distribution of relaxation time shows the dehydra-PEO results in the kinetic tardiness of the charge transfer process in the temporal dimension. Hence, an artificial interface layer (CEI x ) was modified on the surface of NCM811 to regulate the potential of cathode/electrolyte interface to prevent the high-voltage deterioration of PEO. NCM/CEI x /PEO batteries exhibit capacity retentions of 96.0%, 84.6%, and 76.8% after undergoing 100 cycles at cut-off voltages of 4.1, 4.2, and 4.3 V, respectively. Therefore, here the failure mechanism of high-voltage PEO electrolyte is investigated by EIS and a proposed solving strategy is presented.
研究领域:
材料科学
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电化学
·
电池技术
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xuechao Zhang,Haokai Cai,Anping Deng,Hongshuo Chen,Lingfeng Li,Peng Li
Surface Enhanced Raman Spectroscopy (SERS) and Electrochemical Impedance Spectroscopy (EIS) were combined in this work to develop a dual-channel aptamer-based biosensor platform for the detection of aflatoxin B1 (AFB1). The capture probe (Fe 3 O 4 @AuNFs@Aptamer) and the signal probe (AuNStar@4-MBA@Au@cDNA) were chemically synthesized step by step. During the process, SEM, TEM, XRD, Raman and UV-vis were applied to characterize these nanomaterials. The performance of the prepared biosensor was tested in AFB1 solutions. The results showed that the detection range of the SERS channel for AFB1 was 1×10 -4 to 1×10 2 ng/mL, with a limit of detection (LOD) of 7.83×10 -5 ng/mL and the average relative standard deviation (RSD) of the standard samples of 5.24%. And the EIS channel was 1×10 -4 to 1×10 2 ng/mL, with a LOD of 1.58×10 -4 ng/mL and the average RSD of the standard samples of 1.27%. These differences confirm that the combination of SERS/EIS technologies extends the detection limits and improves the reliability of this sensing platform. In summary, this strategy can be adapted to identify other target molecules simply by changing the aptamer, thereby providing a broad and universal platform for biosensing applications.
研究领域:
分析化学
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生物传感器
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食品安全
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纳米技术
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环境监测
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
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IF 7.476
Weilu Liu,Yukai Ma,Guoxiang Sun,Shupiao Wang,Jiayin Deng,Hong Wei
A novel electrochemical biosensor was developed for ultrasensitive determination of testosterone from femtomolar to micromolar levels via electrochemical impedance spectroscopy (EIS) measurements. The sensor features a nanosized molecularly imprinted polymer (MIP) film that was electrochemically grafted on a graphene-oxide sheets modified electrode . The detection mechanism of this senor is explained via the change of the interfacial impedance that derived from the recognition of the target molecule. Due to the nanosheet structure as well as the high surface area of graphene-oxide, the sensitivity of the MIP sensor is enhanced remarkably. Under an optimized condition, a wide linear range from 1 fM to 1 µm (1×10 −15 –1×10 −6 mol L −1 ) and a detection limit of 0.4 fM (4.0×10 –16 mol L −1 ) was obtained. This composite film presented a good selectivity over structurally similar steroid hormones , and a long term stability in room temperature for the detection of testosterone. Considering these advantages, the MIP/GO electrochemical biosensor could be a substitute of testosterone immunosensor, and may be further extended to the detection of other endogenous substances.
研究领域:
生物传感技术
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分析化学
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DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 23
·13742-13748
·
IF 3.108
Fuyou Lu,Baodong Song,Pei He,Zhi Wang,Jixiao Wang
Two component polyurethane coatings are widely used for materials protection. Herein, a series of acrylic polyurethane coatings were prepared by reaction of hydroxyl acrylic resin and hexamethylene diisocyanate (HDI) biuret. These coatings have different isocyanate to hydroxyl ratios (expressed as NCO/OH). The degradation process of the coatings was evaluated by electrochemical impedance spectroscopy measurement. Then, the results were analyzed using equivalent electrical circuits. Besides, the adhesion and water resistance of the coatings were also investigated. Experimental results demonstrated that the higher the NCO/OH ratios within a certain range, the better the corrosion resistance. Specifically, the coating with NCO/OH = 1.4 provided the best corrosion resistance, and its impedance modulus still remained at close to 1 × 1011 ohms per cm2 after 50 days immersion. However, the corrosion resistance of coatings with NCO/OH = 0.8 and NCO/OH = 1.0 were relatively poor. More interestingly, with the increase in NCO/OH ratios, the coating's adhesion increased slightly and the water resistance improved noticeably.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
Materials
·
2020
·Vol. 13
·期 5
·1135
·
IF 3.057
Lei Zha,Heping Li,Ning Wang
TC11 alloy is a promising structural material, and has a wide range of applications in many corrosive and high temperature hydrothermal systems. The passive film has an important influence on its electrochemical behavior. In this study, in-situ electrochemical methods (that is, open circuit potential (OCP), linear polarization (LP) and electrochemical impedance spectroscopy (EIS)) were used to monitor the long period electrochemical behavior of TC11 alloy in 0.01 M Na2SO4solution at 300 °C/10 MPa. The growth kinetics of the passive film was mainly studied. The correlation between the evolution of the electrochemical behavior and the growth of the oxide film was discussed. The results showed that although the OCP gradually stabilized after twenty thousand seconds, henceforth the polarization resistance (Rp) was still increasing due to the thickening of the passive film. An equivalent circuit was proposed to fit the EIS experimental data, leading to determination of film capacitance and film resistance. Besides, the electrochemical data was interpreted in terms of the point defect model (PDM). The EIS results are consistent with theRpresults.Keywords:TC11 alloy;passive film;time;high temperature and pressure;EIS;PDM
研究领域:
材料科学
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电化学
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2020
·Vol. 15
·期 12
·e0244297
·
IF 2.74
Jianhui Zhen,Gang Liang,Ruichun Chen,Wenshen Jia
Acetamiprid (ACE) is a kind of broad-spectrum pesticide that has potential health risk to human beings. Aptamers (Ap-DNA (1)) have a great potential as analytical tools for pesticide detection. In this work, a label-free electrochemical sensing assay for ACE determination is presented by electrochemical impedance spectroscopy (EIS). And the specific binding model between ACE and Ap-DNA (1) was further investigated for the first time. Circular dichroism (CD) spectroscopy and EIS demonstrated that the single strand AP-DNA (1) first formed a loosely secondary structure in Tris-HClO 4 (20 mM, pH = 7.4), and then transformed into a more stable hairpin-like structure when incubated in binding buffer (B-buffer). The formed stem-loop bulge provides the specific capturing sites for ACE, forming ACE/AP-DNA (1) complex, and induced the R CT (charge transfer resistance) increase between the solution-based redox probe [Fe(CN) 6 ] 3−/4− and the electrode surface. The change of Δ R CT (charge transfer resistance change, Δ R CT = R CT(after) - R CT(before) ) is positively related to the ACE level. As a result, the AP-DNA (1) biosensor showed a high sensitivity with the ACE concentration range spanning from 5 nM to 200 mM and a detection limit of 1 nM. The impedimetric AP-DNA (1) sensor also showed good selectivity to ACE over other selected pesticides and exhbited excellent performance in environmental water and orange juice samples analysis, with spiked recoveries in the range of 85.8% to 93.4% in lake water and 83.7% to 89.4% in orange juice. With good performance characteristics of practicality, sensitivity and selectivity, the AP-DNA (1) sensor holds a promising application for the on-site ACE detection.
研究领域:
分析化学
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环境科学
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Maryam Allahyari,Emre Dokuzparmak,Arzum Erdem,Alper AKKAYA,Bahattin Tanyolac
Pseudomonas aeruginosa is a major pathogen responsible for severe infections, particularly in immunocompromised patients and those with chronic conditions. Its increasing resistance to antibiotics underscores the urgent need for effective detection systems. This study introduces a novel biosensor based on bacteriophage (M-PAP1) in combination with MWCNT-COOH modified SPE and its application for the sensitive identification of Pseudomonas aeruginosa . The phage provides high specificity and strong target binding, while the combined ECL and EIS modes offer complementary signal outputs, enhancing analytical reliability and reducing false responses. The ECL sensor achieved LoD of 0.755 CFU ml −1 and demonstrated a broad linear working range of 2.28 to 10 10 CFU ml −1 (R 2 : 0.9981). In artificial urine samples, the sensor demonstrated a recovery rate of 92% to 97%, indicating its effectiveness in real biological matrices. The dual functionality for both ECL and EIS measurements highlights the system's versatility and potential for real-time clinical applications. The system also exhibited exceptional selectivity and minimal interference from non-specific bacteria such as Escherichia coli and Pseudomonas putida and Pseudomonas fluorescens. Overall, the dual-mode strategy significantly strengthens diagnostic accuracy by enabling cross-validated detection signals, offering a robust platform for rapid monitoring of P. aeruginosa in the context of rising antimicrobial resistance.
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DOI
PubMed
高影响因子
Scientific Reports
·
2015
·Vol. 5
·期 1
·1-10
·
IF 5.578
Zong Xianli,Zhu Rong,Guo Xiaoliang
In this paper, a fine gold nanostructure synthesized on selective planar microelectrodes in micro-chip is realized by using an advanced hybrid fabrication approach incorporating growth of nanorods (NRs) with gold electroplating. By this developed nanostructure, integration of in-situ surface-enhanced Raman spectroscopy (SERS) detection with electrochemical impedance spectroscopy (EIS) measurement for label-free, nondestructive, real-time and rapid monitoring on a single cell has been achieved. Moreover, parameters of Au nanostructures such as size of nanoholes/nanogaps can be controllably adjusted in the fabrication. We have demonstrated a SERS enhancement factor of up to ~2.24 × 10 6 and double-layer impedance decrease ratio of 90% ~ 95% at low frequency range below 200 kHz by using nanostructured microelectrodes. SERS detection and in-situ EIS measurement of a trapped single cell by using planar microelectrodes are realized to demonstrate the compatibility, multi-functions, high-sensitivity and simplicity of the micro-chip system. This dual function platform integrating SERS and EIS is of great significance in biological, biochemical and biomedical applications.
研究领域:
纳米技术
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电化学
·
生物传感器
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生物医学工程
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分析化学
·
微流控技术
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·Vol. 17
·期 3
·220327
·
IF 1.5
Zhao-zhan Hou,Qing-xian Yue,Jing Lv,Yu-yu Wang,Bing-qian Zhu,Xuan Liang,Rui Ding,Hai-bin Yu,Xiao Wang,Tai-jiang Gui
As the new type of natural material, Basalt scales (BS) exhibited flaky micron-sized structure. Due to the acid and alkali resistance and stable chemical properties, it was suitable to act as the filler for anti-corrosion coatings. However, BS showed unsatisfactory compatibility with epoxy resins, and its application was limited. This paper combined the basalt scales with the organosilane by covalent grafting, and prepared organosilane modified basalt scales. The modification of organosilane promoted the combination of basalt scales and epoxy resin and the stability of BS in the coatings by covalent bonds, simultaneously improved the anti-corrosion performance of the coatings. Electrochemical impedance spectroscopy (EIS) experiments showed that organosilane significantly improved the anti-corrosion performance of Basalt scales epoxy coatings, especially the organosilane containing amino groups. The promoting effect of organosilane on the stable dispersion of BS was evaluated by the barrier ability of the coatings to corrosive solutions, and the diffusion dynamics analysis implied the existence of the promoting effect. The enhancement of shielding anticorrosion was result of the optimized dispersion and stability of modified basalt scales.
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Chenxi Yi,Benfeng Zhu
The inhibiting behavior of K 4 P 2 O 7 , C 2 H 5 O 6 P and their mixture on Q235 steel corroding in the simulated stratum water has been investigated by using electrochemical noise (EN) and electrochemical impedance spectroscopy (EIS) techniques. The results show that the physical adsorption of KPP onto Q235 surface at 60 °C is somewhat faster than the chemisorption of HPAA. In the re-plotted energy distribution plot, the difference of the region where the EN energy is mostly accumulated may be adopted to distinguish the chemisorption and physical adsorption of inhibitors. Finally, the variation tendency of the charge transfer resistance obtained by EIS technique is just opposite to that of fractal dimension obtained by EN technique, which may hint that the initial corrosion processes of metals can be in-situ monitored by using EN technique expediently.
研究领域:
材料科学
·
电化学工程
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Yan Wu,Tao Liu,Bin Ji,Wei Wang
Iron-cobalt alloy is a potential and economical magnetostrictive material, however, our microscopic understanding of the co-electrodeposition process of Fe-Co alloys is still unsatisfactory. In this work, a flat Fe-Co film with 10 μm was successfully prepared by electrodeposition with a non-toxic sulfate solution. The effects of (NH 4 ) 2 SO 4 , an important additive, were studied and DFT calculation was used to analyze the mechanism of (NH 4 ) 2 SO 4 . EIS shows that (NH 4 ) 2 SO 4 can promote the Fe-Co co-deposition process and obviously inhibits the hydrogen evolution reaction. In addition, (NH 4 ) 2 SO 4 can significantly reduce the internal stress of the Fe-Co film by 110,000 MPa, so that the cracks caused by the internal stress are obviously removed. The results of quantum chemistry calculation further show that NH 4 + is more inclined to adsorb on Fe-Co surface than H 2 O and H + , which reduces the hydrogen evolution on the electrode surface. The results are beneficial to improve the Fe-Co co-deposition process, reduce the internal stress and to prepare a high-performance Fe-Co magnetic film. The films will be assembled on the electronic devices with sensors.
研究领域:
材料科学
·
电化学
·
表面化学
·
量子化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）