Toxins
·
2018
·Vol. 11
·期 1
·6
·
IF 3.273
Ming Li,Mingna Sun,Xia Hong,Jinsheng Duan,Daolin Du
A total of 328 agricultural product samples highly suspected to be contaminated, from flour companies, feed companies, and livestock farms throughout China, were surveyed for deoxynivalenol (DON) contamination using a self-assembly enzyme-linked immunosorbent assay (ELISA) kit. An ELISA kit for DON was developed with a 4.9 ng mL−1limit of detection (LOD) in working buffer and a 200 ng g−1LOD in authentic samples. The DON contamination detection rate was 88.7%, concentrations ranged from 200.9 to 6480.6 ng g−1, and the highest DON contamination was found in distillers’ dried grains with solubles with an average of 3204.5 ng g−1. Wheat bran and wheat were found to be the most commonly contaminated samples, and the corn meal samples had the lowest average DON level. This ELISA kit is a powerful alternative method for the rapid, sensitive, specific, accurate, and high-throughput determination of DON and can meet the maximum requirement levels. This survey suggests that DON contamination in the Chinese market is serious, and the contamination risk deserves attention. Essential preventive measures should be implemented to ensure food safety and human health.Keywords:deoxynivalenol;contamination;survey;ELISA kit;agricultural productKey Contribution:A total of 328 agricultural product samples highly suspected to be contaminated were surveyed for deoxynivalenol using a self-assembly ELISA kit.
研究领域:
食品安全
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Gaoshuang Hu,Ziyang Liu,Yiqin Zhang,Tao Zhao,Zhuqing Xing,Shan Gao,Jianxiong Hao
Long-term consumption of foods with excessive enrofloxacin (ENRO) residues may cause the accumulation of ENRO in the human body, thus damaging human health. In this study, quantum dot-based biomimetic fluorescence immunoassays were used for enrofloxacin detection in food of animal origin. Under the most suitable conditions, the detection limit (IC15) of the method in standard solution was 0.13 ± 0.02 ng L−1 and the sensitivity (IC50) was 0.13 ± 0.18 μg L−1. The recoveries for ENRO from four fortified food samples, including chicken, eggs, shrimp, and milk, ranged from 85.74% to 114.19% and the coefficients of variation were 0.01–18.09%. The established method shows good agreement with the results obtained using commercial ELISA kits, with a correlation coefficient of 0.997. The proposed method shows the advantages of high sensitivity, specificity and wide detection range. It can be used as an alternative method for the rapid and sensitive detection of ENRO in food of animal origin.
研究领域:
食品科学
·
分析化学
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生物技术
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兽医学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 5
·1069-1077
·
IF 2.667
Hu Gaoshuang,Gao Shan,Han Xue,Yang Lixin
Colloidal gold, quantum dots (QDs), and upconversion nanoparticles (UCNPs), which were respectively labeled with monoclonal antibody against norfloxacin (NOR), were employed as signal probes to establish three types of immunochromatographic strips, and the strips were evaluated according to their ability to detect NOR in milk samples. The results showed that the visual detection limit of NOR was 2.0 μg L −1 in the standard solution and 20.0 μg L −1 in milk samples when using colloidal gold-immunochromatographic strips (ICS). When QD-based fluorescence immunochromatographic strips (FICS) were used, the visual detection limit was 2.0 μg L −1 in the standard solution and 10.0 μg L −1 in milk samples. If UCNP-based FICS strips were employed, the visual limit of detection was 0.5 μg L −1 in the standard solution and 2.5 μg L −1 in milk samples. Besides, the results from the proposed methods showed high agreement with results yielded by using commercial ELISA kits, indicating the good accuracy of these strips. Compared with colloidal gold-ICS, QDs-FICS showed similar sensitivity in the standard solution and a two-fold higher sensitivity in milk samples. Meanwhile, UCNPs-FICS yielded the highest sensitivity both in standard solution and in milk samples, and they were also more stable in normal laboratory conditions (that is, they could be exposed to regular room light). Additionally, the two fluorescence nanoparticle-based test strips could be built with less antibody and coating antigens and can be cost-effective. In conclusion, the proposed QDs-FICS and UCNPs-FICS could be employed as cost-effective alternative methods for sensitive, rapid, and on-site detection of NOR in milk samples.
研究领域:
分析化学
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食品安全
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纳米技术
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环境科学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 29
·7127-7138
·
IF 4.3
Liu Shuai,Zhao Jingkai,Wu Junfeng,Wang Ling,Hu Jiandong,Li Shixin,Zhang Hao
A novel deep learning-enabled smartphone platform is developed to assist a colorimetric aptamer biosensor for fast and highly sensitive detection of dimethoate. The colorimetric determination of dimethoate is based on the specific binding of dimethoate and aptamer, which leads to the aggregation of AuNPs in high-concentration NaCl solution, resulting in an obvious color change from red to blue. This color change provides sufficient data for self-learning enabled by a convolutional neural network (CNN) model, which is established to predict dimethoate concentration based on images acquired from a smartphone. To enhance user-friendliness for non-experts, the CNN model is then embedded into a smartphone app, enabling offline detection of dimethoate pesticide in real environments within just 15 min using a pre-configured colorimetric probe. The developed platform exhibits superior performance, achieving a regression coefficient of 0.9992 in the concentration range of 0–10 μM. Moreover, the app’s performance is found to be consistent with the ELISA kit. These remarkable findings demonstrate the potential of combining colorimetric biosensors with smartphone-based deep learning methods for the development of portable and affordable tools for pesticide detection. Graphical Abstract
研究领域:
分析化学
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环境科学
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人工智能
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF VIROLOGICAL METHODS
·
2023
·
IF 3.1
Shuzhen Chen,Qingdong Luan,Shimei Qiu,Yue Zhao,Yanjin Lu,Shufang Sun,Jianlin Wang,Yanbo Yin
To detect the antibody against fowl adenovirus serotype 4 (FAdV-4) in clinical practice, the latex agglutination test (LAT) was developed by using the Fiber-2 protein of FAdV-4 as an antigen bound to sensitized latex microspheres . The concentration, time, and temperature of sensitization latex microspheres by the Fiber-2 protein were studied and optimized; the specificity, sensitivity, and repeatability of LAT were tested; and the method developed in the study was applied. The results showed that the optimum sensitization concentration of Fiber-2 protein was 0.8 mg/mL, the time was 120 min, and the temperature was 37 ℃. Except for antiserum against FAdV-4 and FAdV-10, LAT developed in the study could not agglutinate antisera against FAdV-1, FAdV-2, FAdV-3, FAdV-4, FAdV-5, FAdV-6, FAdV-8a, FAdV-8b, FAdV-11, Newcastle disease virus , infectious bronchitis virus , egg drop syndrome virus and Clostridium perfringens . Compared with the commercial FAdV-4 ELISA Kit, the titers in 21 clinical samples were low when tested by the developed LAT method, but there was no significant difference. The coefficients of variation among different batches and the same batch of latex-sensitized particles were between 0 % and 13.3 % and 0–8.7 %, respectively. The critical value of immune protective antibody against FAdV-4 was 2 5 , and the titers in 40.9 % of clinical samples were higher than the immune critical point. The results showed that the Fiber-2-based LAT developed in the study has the characteristics of high specificity, sensitivity and repeatability, has the advantages of free equipment, long shelf life, and fast and easy operation, and is an effective and convenient method for serological diagnosis of FAdV-4 infection and evaluating the efficacy of vaccines.
研究领域:
兽医学
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微生物学
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免疫学
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诊断方法
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DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 10
·2352-2361
·
IF 4.2
Xiwen Li,Peiran Meng,Mingyang Sun,Yue Chen,Zhiyi Song,Xinyao Wang,Na Li,Yue Sun
Phospholipase C (PLC) has important biological functions in specific cancer types, immune disorders and neurodegeneration. Here, an ultrasensitive electrochemical sensor for PLC was developed via signal amplification based on breathing atom transfer radical polymerization (ATRP). First, phosphatidylethanolamine (PE) was immobilized on the surface of a gold electrode by L-cysteine and cross-linker. Then, PE was specially hydrolyzed by PLC to obtain the phosphate groups and tethered with the ATRP initiator α-bromophenacetic acid (BPAA) by the coordination action of Zr4+. After the breathing ATRP, a large number of electroactive monomers (ferrocenylmethyl methacrylate, FcMMA) were successfully grafted from BPAA. The experimental results indicated that the detection signal of the obtained electrode (sensor) was proportional to the concentration of PLC. The sensor showed a low detection limit of 0.270 U L−1 and a wide linear range of 1–40 U L−1 (R2 = 0.997). Most importantly, the sensor was successfully applied to detect PLC in breast cancer cells (MCF-7, MDA-MB-231) and nontumor cells (MCF-10A). The value obtained by our electrochemical sensor had no obvious difference from that determined by the commercial ELISA kit. These results showed that the fabricated PLC sensor had acceptable potential in clinical applications.
研究领域:
生物化学
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生物技术
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分析化学
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癌症研究
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免疫学
·
神经科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2023
·
IF 4.5
Yong Chang,Meiling Liu,Tong Wu,Ruting Lin,Lin Liu,Qijun Song
Most of proteins in the commercial ELISA kits are recombinant with hexahistidine (His 6 ) tags. Based on this fact, we proposed a simple procedure for the preparation of signal labels and the design of competitive immunosensors. The signal labels were fabricated through the high-affinity interaction between the His 6 tails on the surface of recombinant proteins and the coordinatively unsaturated copper ions on the surface of pristine Cu-based metal organic framework (Cu-MOF). The recombinant protein-modified MOF could be captured by the sensor electrode, thus producing a strong differential pulse voltammetry signal through the electrochemical reduction of Cu 2+ ions in MOF. However, the target protein in sample solution could compete with the His 6 -tagged protein on the surface of Cu-MOF to bind the antibody attached on the electrode, thus leading to the decrease in the electrochemical signal. The peak current showed an inversely relationship with the concentration of target protein. The competitive immunosensor exhibited a linear range of 1 pg/mL to 1 ng/mL with SARS-CoV-2 nucleocapsid protein ( N -protein) as the target analyte. We believe that the method can be used to detect other proteins by utilizing the characteristic of recombinant proteins in commercial kits.
研究领域:
分析化学
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生物化学
·
免疫学
·
电化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Xiwen Li,Zuan Yang,Peiran Meng,Shipeng Jiang,Mingyang Sun,Xiaoyu Zhang,Yue Sun
An ultrasensitive electrochemical sensor for phospholipase C (PLC) was developed via dual signal amplification based on metal-free visible-light induced atom transfer radical polymerization (MVL ATRP) and Ag nanoparticles (AgNPs). The environment-friendly MVL ATRP method was used to form aldehyde based polymers on the electrode surface, providing more reaction sites for further silver mirror reaction. In addition, a large number of electrically active AgNPs were obtained through efficient and low-cost silver mirror reaction, which improved the detection signal. Under the optimal conditions, the proposed sensor showed a linear response from 1 mU/L to 10 6 mU/L for PLC with a detection limit of 0.1032 mU/L (S/N = 3). Most importantly, the sensor was successfully applied to detect PLC in non-tumor cells (MCF-10A) and tumor cells (MCF-7, MDA-MB-231). The obtained values had no obvious difference with that determined by commercial ELISA kit. When 0.5–10 U/L standard PLC were added into the above cells extracts, the recovery rate was between 96.1% and 102.9%, and the RSD was less than 4.02%. The results showed that the fabricated PLC sensor had acceptable potential in clinical applications.
研究领域:
分析化学
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生物化学
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纳米技术
·
生物医学工程
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 10
·1-9
·
IF 6.232
Huang Lingting,Wang Jun,Wang Qingshui,Tang Dianping,Lin Yao
A paper-based visual fluorescence immunoassay is presented for the detection of matrix metalloproteinase-7 (MMP7) that is related to renal cancer. The method is based on the distance-dependent fluorescence quenching of CdTe quantum dots (QDs) on a nitrocellulose membrane by Ag + following a sandwich-type immunoreaction on microtiter wells using silver nanoparticle (AgNP)–labeled secondary antibody– and primary antibody–coated microtiter wells. The silver nanoparticles captured in the well are dissolved with HNO 3 , while the quenching effect of QDs is based on silver ion-exchange reaction under 365-nm excitation light irradiation. Increasing concentration of released Ag + , thus higher concentration of the protein, leads to an increased distance of quenching on the nitrocellulose membrane. The paper-based immunoassay by combination of AgNP-assisted ion-exchange reaction with QD gives good distance-dependent responses and allows the detection of MMP7 at a concentration as low as 7.3 pg mL −1 . The coefficients of variation are less than 6.9% and 12.4% for intra-assay and inter-assay, respectively. High specificity and long-term stability are achieved during the assay. Importantly, the testing of human serum samples using our strategy shows well-matched results with commercial human MMP7 ELISA kits.
A distance-dependent visual immunoassay is developed for the determination of serum matrix metalloproteinase-7 on CdTe quantum dot–impregnated paper with silver ion-exchange reaction.
研究领域:
分析化学
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生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Jialun Chen,Ping Tong,Lingting Huang,Zhonghua Yu,Dianping Tang
An interdigitated capacitance immunosensing system based enzymatic biocatalytic precipitation on micro-comb electrode was designed for the sensitive detection of prostate-specific antigen (PSA) by coupling Ti 3 C 2 MXenes with tyramine signal amplification strategy. The immunosensor was prepared by immobilizing anti-PSA capture antibody on MXenes-coated interdigitated electrode, whereas gold nanoparticles heavily functionalized with horseradish peroxidase (HRP) and detection antibody were utilized as the signal-transducer tags. Introduction of interdigitated electrode was expected to enhance the sensitivity of capacitance immunosensor. This system mainly consisted of the sandwich-type immunoreaction, formation of tyramine-HRP repeats on gold nanoparticle and enzymatic biocatalytic precipitation. The concatenated HRP through the tyramine oxidized numerous 4-chloro-1-naphthol molecules into insoluble benzo-4-chlorohexadienone with the help of H 2 O 2 , and coated the modified immunosensor to keep free ions away from the electrode, thus causing the local alteration in the capacitance. Under optimum conditions, the change of the immunosensor in the capacitance increased with the increasing target PSA concentrations from 0.1 ng mL −1 to 50 ng mL −1 at a detection limit of 0.031 ng mL −1 . Moreover, the interdigitated capacitance immunosensor showed good reproducibility, high specificity and acceptable accuracy for the analysis of human serum specimens in comparison with those obtained from commercial human PSA ELISA kit. Importantly, Ti 3 C 2 MXenes-based interdigitated capacitance transducer open new opportunities for protein diagnostics and biosecurity.
研究领域:
生物传感器
·
分析化学
·
纳米技术
·
蛋白质诊断
·
生物安全
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Xiaoxia Xie,Zhenyun He,Chaoshuang Qu,Zhichang Sun,Hongmei Cao,Xing Liu
Hazardous small molecules in food and environment seriously threatens human health, which requires sensitive and rapid tools for monitoring. Using a previously identified nanobody against ochratoxin A (OTA), we herein proposed a homogeneous sensing platform “nanobody/NanoLuc Binary Technology (NanoBiT) system” and developed a nanobody/NanoBiT system-mediated bioluminescence immunosensor (NBL-Immunosens) for OTA using LgBiT (Lg) and SmBiT (Sm), two subunits of the split nanoluciferase (NanoLuc). The core elements of NBL-Immunosens include Lg-nanobody fusion (NLg) and Sm-labeled OTA-bovine serum albumin conjugate (OSm). The antigen-antibody interaction between NLg and OSm triggers the reconstitution of NanoLuc for generating luminescence signals. Moreover, free OTA can compete with OSm for binding to NLg, resulting the decrease of dose-dependent signals. NBL-Immunosens can detect OTA in a one-step assay of 5 min without washing and exhibit a limit of detection of 0.01 ng/mL with a linear range of 0.04–2.23 ng/mL. It shows high selectivity for OTA and has good accuracy and precision in the spiking-and-recovery experiments. Furthermore, its effectiveness was evaluated with real cereal samples and confirmed by liquid chromatography tandem mass spectrometry and commercial ELISA kits. Hence, the NBL-Immunosens is a very promising tool for rapid, accurate, and selective detection of trace OTA in food.
研究领域:
食品科学
·
分析化学
·
毒理学
·
生物传感器
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 413
·期 1
·235-244
·
IF 3.637
Guo Haixin,Su Xiaoping,Su Qingfu,Zhuang Wei,You Zhijiao
A portable photothermal immunoassay based on Au-coated magnetic Fe 3 O 4 core-shell nanohybrids (Au-Fe 3 O 4 ) was developed for point-of-care (POC) testing of lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ) on a digital near-infrared (NIR) thermometer. Au-Fe 3 O 4 photothermal materials were first synthesized through reverse micelle method, and then functionalized with polyclonal rabbit anti-human Lp-PLA 2 antibody. A sandwiched immunoreaction was carried out in polyclonal mouse anti-human Lp-PLA 2 antibody-coated microplate using Au-Fe 3 O 4 -labeled antibody as the detection antibody. With formation of sandwich-type immunocomplex, the captured Au-Fe 3 O 4 on the plate converted the light into heat under an 808-nm laser irradiation (1.5 W cm −2 ), thereby resulting in the increasing temperature of the detection solution. The temperature variations relative to surrounding temperature was determined on a portable NIR thermometer. Several labeling protocols with gold nanoparticle, Fe 3 O 4 nanoparticle, or Au-Fe 3 O 4 nanohybrids were investigated for determination of Lp-PLA 2 and improved analytical features were achieved with the core-shell Au-Fe 3 O 4 nanohybrids. Under optimum conditions, Au-Fe 3 O 4 -based immunoassay exhibited good photothermal responses for the detection of Lp-PLA 2 with a dynamic linear range of 0.01–100 ng mL −1 at a low detection limit of 8.6 pg mL −1 . Good reproducibility and intermediate precision were less than 9.7%. Other biomarkers or proteins did not interfere with responses of this system. An acceptable accuracy was acquired for analysis of human serum sample between Au-Fe 3 O 4 -based photothermal immunoassay and commercialized human Lp-PLA 2 ELISA kit.
研究领域:
纳米技术
·
生物医学工程
·
分析化学
·
即时检测
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）