The effect of drying methods on the variations of volatile, semi-volatile, and involatile compounds in Elaeagnus angustifolia L. (E.A.) flower was comprehensively investigated by using gas chromatography-mass spectrometry (GC-MS) and ultrahigh performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) based untargeted metabolomics combined with our recently developed automatic data analysis software AntDAS for the first time. About 21 volatile compounds, 44 semi-volatile compounds, and 17 involatile compounds were identified and showed significant differences among drying methods. Chemometric methods like principal components analysis (PCA), and partial least squares-discriminant analysis (PLS-DA) were used in combination with heatmap analysis to reveal the relationship between compound compositions and drying methods. Finally, it was concluded that room-temperature drying is more conducive to preserving volatile compounds in E.A. flower, and hot air drying is better for preserving semi-volatile and involatile compounds. This work can provide guidelines for industrial processes of E.A. flower.
Development of polysaccharide biomass for efficient electrocatalytic sensing applications is important for its natural resource utilization and detection application. Elaeagnus angustifolia L. gum is chosen as an electron-rich macromolecule of polysaccharide as the encapsulating agent to surround etched parted ZIF-67(Co) particles. The three electron-rich molecules trithiocyanuric acid, urea, and sodium hypophosphite are selected as the weak acid etching agents, which could etch ZIF-67(Co) microcubes. This fragmentation of ZIF-67(Co) is attributed to the electron-rich molecules disrupting the internal coordination bonds of ZIF-67(Co), thus producing fragmented particles with increased exposed active electrocatalytic spots. Further carbonization of the mixture is processed to obtain the electrocatalyst ZIF-67(Co)-NPS-doped biomass carbon (ZNBC). The ZNBC/GCE sensor shows a significant improvement in electrochemical performance with a linear range of 0.5 to 100 μM, a sensitivity of 10.04 μA μM–1 cm–2, a limit of detection of 0.052 μM, and good selectivity. The ZNBC/GCE sensor was also used in rapid and accurate electrochemical sensing of APAP in urine, lake water, and pharmaceutical capsule preparation samples with good recoveries. This electron-rich macromolecule/molecule as an encapsulating/etching combo strategy provides insight into the usage of Elaeagnus angustifolia L. gum and top-down design of metal–organic frameworks (MOFs) for potential applications in electrocatalyst design.
Hongtao Du,Juncheng Chen,Shan Tian,Hongling Gu,Na Li,Yao Sun,Jiajia Ru,Junru Wang
In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82 ± 0.38%, which is in good agreement with the predicted value (9.93 ± 0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70 kDa and 4.39 kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400 μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods .
危险品化学品经营许可证（带存储）