AQUATIC TOXICOLOGY
·
2014
·
IF 3.513
Tao Wang,Xiaohua Long,Yongzhou Cheng,Zhaopu Liu,Shaohua Yan
Copper nanoparticles (Cu-NPs) were widely used in various industrial and commercial applications. In this study the effects of Cu-NPs and soluble Cu were investigated on juvenile Epinephelus coioides . The fish were exposed in triplicate to control, 20 or 100 μg Cu L −1 as either copper sulphate (CuSO 4 ) or Cu-NPs in a semi-static aqueous culture for 25 days. The growth parameters were significantly lower at 100 μg Cu L −1 as CuSO 4 or Cu-NPs treatment compared to control. Time-dependent Cu accumulation in all tissues increased with increasing the Cu dose. The percentage of total Cu found in remaining tissues (head, bones, fins, etc.) decreased more in the CuSO 4 than Cu-NPs treatment after 25 days, but increased in all other tissues (especially in liver). Compared with the control, either Cu-NPs or CuSO 4 induced higher malonaldehyde concentration in tissues by overwhelming total superoxide dismutase activity, total glutathione concentration and Na + /K + -ATPase activity, but the opposite results were recorded for the brain. With increasing the CuSO 4 or Cu-NPs dose, apoptosis was exacerbated in the liver and gills, more so by CuSO 4 than Cu-NPs. Overall, these findings showed that Cu-NPs had the toxic effects similar to dissolved Cu; hence, Cu-NPs need to be included in the assessment of toxicological impacts in the aquatic environment.
研究领域:
环境科学
·
毒理学
·
水产养殖学
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Genomics
·
2015
·
IF 0.953
Tao Wang,Xiaohua Long,Yongzhou Cheng,Zhaopu Liu,Shaohua Yan
Copper nanoparticles (Cu-NPs) are components in numerous commercial products, but little is known about their potential hazard in the marine environments. In this study the effects of Cu-NPs and soluble Cu on juvenile Epinephelus coioides were investigated. The fish were exposed in triplicate to control, 20 or 100 µg Cu L−1 as either copper sulphate (CuSO4) or Cu-NPs for 25 days. The growth performance decreased with increasing CuSO4 or Cu-NPs dose, more so in the CuSO4 than Cu-NPs treatment. Both forms of Cu exposure inhibited activities of digestive enzymes (protease, amylase, and lipase) found in liver, stomach, and intestine. With an increase in CuSO4 and Cu-NPs dose, crude protein and crude lipid decreased, but ash and moisture increased, more so in the CuSO4 than Cu-NPs treatment. The Cu-NPs treatment caused pathologies in liver and gills, and the kinds of pathologies were broadly of the same type as with CuSO4. With an increase in CuSO4 or Cu-NPs dose, the total polyunsaturated fatty acids decreased, but total monounsaturated fatty acids and total saturated fatty acids increased compared to control. Overall, these data showed that Cu-NPs had a similar type of toxic effects as CuSO4, but soluble Cu was more toxic than Cu-NPs.
研究领域:
海洋生物学
·
环境科学
·
水产养殖
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
PLoS One
·
2016
·Vol. 11
·期 2
·e0149484
·
IF 3.057
Tao Wang,Xiaoyan Chen,Xiaohua Long,Zhaopu Liu,Shaohua Yan
Copper nanoparticles (Cu-NPs) were widely used in various industrial and commercial applications. The aim of this study was to analyze the cytotoxicity of Cu-NPs on primary hepatocytes of E . coioides compared with copper sulphate (CuSO 4 ). Cultured cells were exposed to 0 or 2.4 mg Cu L -1 as CuSO 4 or Cu-NPs for 24-h. Results showed either form of Cu caused a dramatic loss in cell viability, more so in the CuSO 4 than Cu-NPs treatment. Compared to control, either CuSO 4 or Cu-NPs significantly increased reactive oxygen species(ROS) and malondialdehyde(MDA) concentration in hepatocytes by overwhelming total superoxide dismutase (T-SOD) activity, catalase(CAT) activity and glutathione(GSH) concentration. In addition, the antioxidative-related genes [ SOD (Cu/Zn) , SOD (Mn) , CAT , GPx4 ] were also down-regulated. The apoptosis and necrosis percentage was significantly higher after the CuSO 4 or Cu-NPs treatment than the control. The apoptosis was induced by the increased cytochrome c concentration in the cytosol and elevated caspase-3, caspase-8 and caspase-9 activities. Additionally, the apoptosis-related genes ( p53 , p38β and TNF-α ) and protein (p53 protein) were up-regulated after the CuSO 4 or Cu-NPs treatment, with CuSO 4 exposure having a greater effect than Cu-NPs. In conclusion, Cu-NPs had similar types of toxic effects as CuSO 4 on primary hepatocytes of E . coioides , but toxicity of CuSO 4 was more severe than that of Cu-NPs.
研究领域:
环境毒理学
·
海洋生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Nanotoxicology
·
2017
·
IF 6.428
Tao Wang,Xiaohua Long,Xiaoyan Chen,Yuanrui Liu,Zhaopu Liu,Shiqun Han,Shaohua Yan
Copper nanoparticles (Cu-NPs) are components in numerous commercial products, but little is known about the mechanisms of their toxicity to marine fish. Here, we investigated physiology, transcriptome and proteome in Epinephelus coioides after exposure to Cu as Cu-NPs or copper sulfate (CuSO4). Aggregation, oxidation and dissolution of Cu-NPs occurred after suspension in seawater within 24 h. Cu-NPs had similar types of the histology and hematological effects as CuSO4 on E. coioides, but toxicity of Cu-NPs seems more severe than that of CuSO4. Venn diagram analyses revealed 1428 and 2239 genes with significantly altered regulation in, respectively, CuSO4 and Cu-NPs treatments; of these, 911 genes were common to both treatments. A total of 354 and 140 proteins with significantly altered regulation were identified in, respectively, CuSO4 and Cu-NPs treatments; of these, 75 proteins were common to both treatments. A total of 11,417 transcripts and 3210 proteins were assigned to gene ontology terms, clusters of orthologous groups and Kyoto encyclopedia of genes and genomes. Correlation analysis of gene and protein expressions revealed that 21 differentially expressed proteins had their regulation changed in the same direction in both Cu-NPs and CuSO4 treatments. Those genes and proteins could be used as targets for subsequent analysis, regardless of the Cu form. Among those proteins, one of the most notable changes was in proteins related to lipid transport and metabolism. This study provides an enhanced understanding of E. coioides responses to Cu-NPs or CuSO4.
研究领域:
海洋生物学
·
毒理学
·
分子生物学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FISH DISEASES
·
2022
·Vol. 46
·期 3
·215-227
·
IF 2.58
Ziqing Zhong,Xiping Wu,Mindong Bai,Xiaodian Huang,Qilin Zheng,Chunxiang Ai
Cryptocaryon irritans causes one of the most serious diseases in various wild and cultured marine fish, leading to mass mortality and economic loss. In this study, hydroxyl radical (•OH) solution produced by strong ionization discharge combined with water jet cavitation effect was injected into orange-spotted grouper ( Epinephelus coioides ) aquaculture tanks for C. irritans control. The results showed that all C. irritans theronts were inactivated by •OH solution at concentrations of 0.5 mg/L within 2 min. •OH could induce alteration of shape, the absence of motility and macronucleus dispersion in theronts. A possible explanation was that the macronucleus of C. irritans might be damaged by •OH; as a result, its metabolism and life activities were disturbed. The •OH treatment increased the survival rate of E. coioides challenged with C. irritans from 64.7 ± 8.0% (mean ± SD) to 100% and reduced their infection intensity significantly. Stress response biomarkers such as malonaldehyde, glutathione, glutathione peroxidase, superoxide dismutase (SOD) and catalase levels in the gills of E. coioides at different time points were analysed. The SOD activity in the •OH group first decreased and then recovered to the initial level at the end of the experiment. The other stress response biomarkers had no significant difference from that in the uninfected control group after •OH treatment. Additionally, the gill of E. coioides in the •OH group exhibited slight and reversible transformation compared with the uninfected control group. Compared with •OH treatment, chlorine dioxide and formalin treatment reduced the survival rate, induced oxidative damage and changed the histological gill structure in E. coioides . In conclusion, •OH could be applied effectively to control C. irritans infection without affecting the normal physiological condition of E. coioides .
研究领域:
水产养殖学
·
寄生虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2015
·
IF 2.674
Tao Wang,Xiaohua Long,Zhaopu Liu,Yongzhou Cheng,Shaohua Yan
Copper nanoparticles (Cu-NPs) are widely used in various industrial and commercial applications, but little is known about their potential hazard in the intestines of marine teleosts. In this study we investigated the effects of Cu-NPs and soluble Cu in the intestines of juvenile Epinephelus coioides . The fish were exposed in triplicate to control, 20 or 100 μg Cu L −1 as either copper sulphate (CuSO 4 ) or Cu-NPs for 25 days. With an increase in Cu-NPs or CuSO 4 dose, the concentration of malonaldehyde in the intestines significantly increased, whereas the activities of total superoxide dismutase and catalase as well as glutathione concentration decreased compared to the control. Statistical analysis of an apoptosis index of intestinal cells showed that general dose-dependent apoptosis was induced by Cu-NPs or CuSO 4 , with Cu-NPs inducing the significantly higher apoptosis index than CuSO 4 . Caspase-3 and caspase-9 activities were increased with an increase in Cu-NPs or CuSO 4 dose, more so in the Cu-NPs than CuSO 4 treatment. With an increase in Cu-NPs or CuSO 4 dose, succinate dehydrogenase and Na + -K + -ATPase activity and cytochrome c concentration in mitochondria decreased, accompanied by increased cytochrome c concentration in the cytosol. Concentration of heat shock proteins 70 and 90 in the intestines and expression of corresponding genes were enhanced with an increase in the Cu-NPs or CuSO 4 dose, but the concentrations and expressions of immunoglobulin M and lysozyme decreased (more in the Cu-NPs than CuSO 4 treatment) compared to the control. Expression of interleukin-1beta and tumor necrosis factor-alpha showed a dose-dependent increase with the increased Cu-NPs or CuSO 4 dose, with the highest expression found in the Cu-NPs treatment. In conclusion, Cu-NPs had similar toxic effects as CuSO 4 in the intestines of juvenile E. coioides , but toxicity of Cu-NPs was more severe than that of CuSO 4 .
研究领域:
环境毒理学
·
水产养殖学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）