ENVIRONMENTAL POLLUTION
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2018
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IF 4.358
Luyao Zhang,Jinling Diao,Li Chen,Zikang Wang,Wenjun Zhang,Yao Li,Zhongnan Tian,Zhiqiang Zhou
Glufosinate-ammonium (GLA) is a spectrum herbicide that is widely used in agriculture. The toxic effects of GLA on plants and mammals have been extensively studied; however, little is known about its effects on reptiles. In this study, male lizards ( Eremias argus ) were exposed to GLA contaminated soil for 60 days. Physical conditions, organ coefficients, antioxidant enzyme activity , tissue distribution, histopathological damage, steroid hormones levels, and related gene expression of sex steroids were evaluated. In contrast to unexposed control lizards, the body mass index of the GLA group was decreased, which elucidated that GLA adversely affected the physical condition of E. argus . Changes in antioxidant enzyme activities in response to elevated malondialdehyde levels in lizard testis indicated that testes were strongly affected by oxidative damage, and the increased testis index was associated with severe testis lesions. Moreover, alterations of plasma sex hormone levels and related gene expression levels of sex steroids were also observed, and the mechanism underlying the induction of reproductive toxicity was clarified. The activity of glutamine synthetase was severely inhibited in the liver of the GLA exposure group. Based on the results of liver index and histopathology examinations, the hepatotoxicity effect of GLA was confirmed.
研究领域:
环境毒理学
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生殖生物学
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生态毒理学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
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2019
·
IF 5.108
Luyao Zhang,Li Chen,Zhiyuan Meng,Wenjun Zhang,Xin Xu,Zikang Wang,Yinan Qin,Yue Deng,Rui Liu,Zhiqiang Zhou,Jinling Diao
Reptiles, the most diverse taxon of terrestrial vertebrates, might be particularly vulnerable to soil pollution. Reptiles especially lizards have been rarely evaluated in ecotoxicological studies, and there is a very limited report for effects of soil pesticide contaminants on lizards. In this study, male and female lizards ( Eremias argus ) were exposed to Glufosinate-ammonium (GLA) and l - Glufosinate-ammonium (L-GLA) for 60 days. Slower sprint speed, higher frequency of turning back and reduced brain index were observed in treatment groups. The accumulation of GLA in the brain of lizard was higher than that of L-GLA. Moreover, the activities of neurotoxicity-related enzymes and biomarkers of oxidative stress were also investigated. In summary, the neurotoxic effects of lizards have been observed after exposure to GLA and L-GLA. Based on the result of the Integrated Biomarker Response (IBR), males were more sensitive to contaminants than females. On the other hand, the neurotoxic pathways by GLA and L-GLA triggered were slightly different: GLA mainly acted on glutamine synthetase (GS), acetylcholinesterase (AchE) and Catalase (CAT) and L-GLA aimed at AchE, Na + /K + -ATPase, Superoxide dismutase (SOD) and Malondialdehyde (MDA). In summary, the accumulation of GLA and L-GLA in lizard's brain induced neurotoxicity by altering the levels of enzymes related to nervous system and antioxidant activity and further resulted in the decrease of brain index and locomotor performance.
研究领域:
生态毒理学
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爬行动物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 27
·期 8
·7823-7834
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IF 2.914
Wang Zikang,Tian Zhongnan,Chen Li,Zhang Wenjun,Zhang Luyao,Li Yao,Diao Jinling,Zhou Zhiqiang
Reptiles are an important part of vertebrates and are the primitive terrestrial vertebrates. However, lots of reptile species are endangered or susceptible to extinction. It is no doubt that contaminants are one of the important reasons for the decline of the lizard population. In this study, the selective metabolism of triadimenol (TN) in the male Eremias argus lizards and the toxic effects of TN on lizards were studied. TN chiral isomers were separated and detected by HPLC-MS/MS system with Lux Cellulose-1 column. Tissue distribution experiments showed the existence of stereoselectivity biotransformation of TN enantiomers among organs in lizards, and RR-TN preferentially emerged over the other enantiomers. The antioxidant enzymes (SOD, CAT, GST) activities and MDA content assays demonstrated that TN induced oxidative stress in most organs, especially in the liver, and the histopathology analysis showed the severe liver and testis damage caused by 14-day continuous TN gavage. The reproductive effects of TN-induced reflected in the increased sex hormone testosterone. This research confirms that TN could induce hepatic and reproductive toxicity of E. argus lizard.
研究领域:
环境毒理学
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爬行动物生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2020
·
IF 6.793
Luyao Zhang,Zhiyuan Meng,Li Chen,Guiting Zhang,Wenjun Zhang,Zhongnan Tian,Zikang Wang,Simin Yu,Zhiqiang Zhou,Jinling Diao
The trade-off between self-maintenance and reproduction has been explored wildly in reptiles. However, the effects of exogenous pollutants on the life history traits of reptiles have not been paid attention to. In the current study, lizards ( Eremias argus ), living in the soil polluted by perfluorooctanoic acid (PFOA) were selected as the main focus. Bodyweight, survival rate, clutch characteristics and biochemical analysis (immune response, lipid accumulation, sex steroid secretion, antioxidant level, and metabolomics) were investigated and the results revealed that lizards’ life-history trade-offs are gender-dependent: females were more inclined to choose a “Conservative” life-history strategy. After 60 days of exposure to PFOA, larger body weight, higher survival rate, stronger immune response, and lighter egg mass in females suggested that their trade-offs are more biased towards self-maintenance. Whereas, the “Risk” strategy would more popular among males: reduced body weight and survival rate, and suffering from oxidative damage indicated that males made little investment in self-maintenance.
研究领域:
生态学
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毒理学
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生殖生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2019
·
IF 5.714
Luyao Zhang,Li Chen,Zhiyuan Meng,Ming Jia,Ruisheng Li,Sen Yan,Sinuo Tian,Zhiqiang Zhou,Jinling Diao
In the context of global warming , an important issue is that many pesticides become more toxic, putting non-target organisms at higher risk of pesticide exposure. Eremias argus (a native Chinese lizard) was selected as animal model in this study. As a kind of poikilothermic vertebrate, E.argus is sensitive to temperature change. The experimental design [(with or without L-Glufosinate-ammonium (L-GLA) pollution × two temperatures (25 and 30 °C)] was used in this study for 90 days to identify the chronic effects of the pesticide–temperature interaction on the lizards’ neuroendocrine-regulated reproduction. Survival rate, body weight, clutch characteristics, testicular histopathology , the content of neurotransmitters and related enzyme activity , the level of sex steroid, the expression of Heat shock protein 70 (HSP70), antioxidant system, the accumulation and degradation of L-GLA were examined. Results showed that L-GLA disrupt reproduction of lizards through hypothalamus-pituitary-gonad (HPG) axis. In addition, temperature can not only change the environmental behavior of pesticides, but also alter the physiological characteristics of lizards. Thus, our results emphasized that temperature is an essential abiotic factor that should not be overlooked in ecotoxicological studies.
研究领域:
生态毒理学
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环境科学
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生殖生物学
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神经内分泌学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Yufan Nie,Simin Yu,Luyao Zhang,Zikang Wang,Rui Liu,Yuping Liu,Wentao Zhu,Zhiqiang Zhou,Jinling Diao
Declines in reptile populations due to climate warming and environmental pollution have been documented. Although recent ecotoxicological studies of reptiles have increased, little is known about how these two stressors interact to affect reptile reproductive processes. This study investigated the single and combined effects of atrazine and warming on reproduction in female lizards (Eremias argus) following 10 weeks of exposure to environmentally relevant concentrations of atrazine (0–10 mg·kg–1) at two temperature treatments (control or warming). Reproductive traits, clutch characteristics, and endpoints related to endocrine disruption (HPG axis gene expression) and energy metabolism (enzyme activity, hepatic metabolomics) were assessed. Atrazine inhibits female reproduction by disrupting HPG axis-related gene expression and energy metabolism, resulting in delayed spawning time and reduced fecundity. In contrast, warming promoted female reproduction and partly alleviated the inhibitory effects of atrazine, which is related to the upregulation of HPG axis-related gene expression and an additional energy metabolism compensatory response. Additionally, atrazine and/or warming altered the direction and intensity of the trade-off between egg number and size and affected maternal nutritional investment in eggs. These findings highlight the complex interplay of environmental stressors on lizard reproduction and add to a better understanding of reptile reproductive strategies and ecological consequences under environmental stress.
研究领域:
生态学
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毒理学
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内分泌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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