PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2025
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IF 1.9
Yang Li,Wei Zhao,Qifeng Li,Jiayu Zhang,Anquan Yang,Min Xie,Ranhua Xiong,Chaobo Huang
Erigeron breviscapus, a medicinal plant rich in flavonoids with diverse pharmacological properties is an important resource. To maximize the utilization of these valuable bioactive compounds, ultrasound-assisted extraction combined with response surface methodology was used to optimize the extraction conditions for total flavonoids. After comparing six macroporous resins’ adsorption and desorption characteristics, AB-8 was selected for enriching total flavonoids, and its adsorption kinetics and thermodynamics were investigated. The following parameters were chosen as optimal for the dynamic adsorption/desorption experiments using an AB-8 filled column: sample loading concentration of 1.14 mg/mL, sample loading flow rate of 2 BV/h, elution solvent of 70% ethanol, desorption flow rate of 1 BV/h, elution solvent volume of 5.5 BV, pH of 2, temperature of 25 °C. After enrichment, the concentration of flavonoids was 5.16 times higher than that of crude extract. LC-MS identified 54 flavonoid molecules with excellent antioxidant properties.
研究领域:
植物化学
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药学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
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2018
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IF 3.677
Chen Ruibing,Chen Xianghui,Zhu Tingting,Liu Jianghua,Xiang Xing,Yu Jian,Tan Hexin,Gao Shouhong,Li Qing,Fang Yichao,Chen Wansheng,Zhang Lei,Huang Baokang
Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse, E. breviscapus is facing the problems of extinction and habitat degradation. In this study, a correlation analysis between the transcript and metabolite profiles of methyl jasmonate (MeJA)-treated E. breviscapus at different time points indicated that chalcone isomerase (EbCHI) was the primary contributor to scutellarin accumulation during flavonoid biosynthesis. EbCHI was then further characterized as a chalcone isomerase that efficiently converted chalcone to naringenin in vitro. Optimal parameters derived by comparing different culture conditions were successfully used to establish hairy root cultures of E. breviscapus with a maximum transformation rate of 60% in B5 medium. Furthermore, overexpression of EbCHI significantly enhanced scutellarin accumulation in E. breviscapus hairy roots with a maximum content of 2.21 mg g-1 (dw), 10-fold higher than that of natural roots (0.21 mg g-1 dw). This study sheds new light on a method of effective gene-based metabolic engineering by accurate and appropriate strategies and provides a protocol for hairy root cultures that accumulate high levels of scutellarin, providing a promising prospect for relieving the overexploitation and unavailability of E. breviscapus in the future.
研究领域:
植物生物技术
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植物化学
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代谢工程
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2025
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IF 7.3
Leshou Zhang,Lili Yu,Shengchun Yang,Xiaofang Yang,Shaodui Ye,Haiwei Ma,Jiayi Hu,Yong Liu,Zhenjuan Duan,Xiaodong Wen
Natural deep eutectic solvents (NADESs), as a newly developed class of environmentally benign solvents, offer a promising alternative to conventional toxic organic solvents for the extraction of valuable bioactive compounds from medicinal plants. This study developed an innovative green approach for the simultaneous extraction of active ingredients from Erigeron breviscapus (E. breviscapus) by utilizing ultrasound-assisted natural deep eutectic solvent extraction (UAE-NADES). Response surface methodology was employed to systematically optimize the extraction parameters. Under the optimized extraction conditions, the total extraction yield of phenolic acids and flavonoids obtained using UAE-NADES was 68.66 mg/g, demonstrating superior performance compared to that of traditional organic solvents. Additionally, the extraction mechanism of the representative bioactive constituent, scutellarin, established by the proposed method was investigated by using density functional theory (DFT), revealing that hydrogen-bonding and van der Waals forces exerted a significant influence on the extraction process. More importantly, NADES exhibits the capacity for multiple recycling and reuse cycles. Finally, the UAE-NADES strategy was applied to achieve the simultaneous extraction of target bioactive constituents from E. breviscapus under varying light qualities following supplementary lighting. This study provides a novel strategy for the green, sustainable, and simultaneous extraction of multiple bioactive constituents from medicinal plants via recyclable NADES.
研究领域:
绿色化学
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植物化学
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提取技术
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天然产物
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计算化学
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可持续工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）