Evidence-based Complementary and Alternative Medicine
·
2017
·
IF 1.74
Feng Li,Yijia Li,Qingxian Li,Xianai Shi,Yanghao Guo
The interest focusing on Eriobotrya japonica leaf triterpene acid (ELTA) has increased recently because of its beneficial effects on health. However, there has been a lack of experimental data on its toxicity. The present study therefore was conducted to evaluate its toxicity in ICR mice. The results showed that ELTA produced neither mortality nor toxicity of the main organs in ICR male and female mice in both acute (0.30, 0.65, 1.39, and 3.00 g·kg−1 body weight) and subacute (150, 300, and 600 mg·kg−1 BW) 28-day toxicity studies. Because of lacking apparently adverse effects found in the hematology, clinical biochemistry, and histopathology evaluation, results of the present study together with the beneficial effects make ELTA as a promising dietary supplement and indicated that ELTA administered orally might have a large safety margin for human.
研究领域:
药理学
·
毒理学
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DOI
PubMed
高影响因子
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
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2018
·
IF 0.715
Ti Wu,Shoukai Lin,Bisha Wu,Yu Chen,Suying Lin,Dahe Lin,Linxia Chen,Zhisheng Zhang,Jincheng Wu
Cold-stored fruits of the loquat (Eriobotrya japonica Lindl.) plant suffer from strong lignification, which leads to a hard texture and a crude mouthfeel of the pulp. However, the signalling mechanisms of loquat fruits under cold storage remain unclear. In this study, the heterotrimeric G protein α subunit (Gα) gene (EjLGA1) from loquat was isolated, and in silico analysis was performed. The levels of EjLGA1 expression in cold-stored loquat fruits with different pretreatments were also investigated. The largest open-reading frame of EjLGA1 is 1173 bp, encoding a polypeptide of 390 amino acids. The nucleotide and amino acid sequences of the Gα subunit are highly conserved among loquat and 48 other seed plants, indicating that the loquat Gα subunit likely has similar functions in other plants. A reliable model of the loquat Gα subunit was constructed, which revealed the structure of the subunit. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results showed that EjLGA1 is highly expressed under cold storage, which suggests the significance of EjLGA1 function in loquat fruits in response to cold stress. However, ethephon and 1-methylcyclopropene (1-MCP) pretreatments did not regulate EjLGA1expression. These results provide new insight into the signalling mechanism in cold-stored loquat fruits.
研究领域:
植物学
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分子生物学
·
食品科学
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DOI
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Xinya Liu,Liqin Song,Baogui Xue,Zhuoheng Chi,Yuan Wang,Songqin Wen,Wenjuan Lv,Qiankun Hu,Qigao Guo,Shuming Wang,Di Wu,Guolu Liang,Danlong Jing
Triploid loquats are divided into yellow- and white-fleshed cultivars. To better understand taste variations in triploid loquat fruits, we used a UPLC-ESI-QTRAP-MS/MS-based widely targeted metabolomic analysis to examine the metabolic composition of two different color fleshed triploid loquats with a sample size of 54 and external validation method within a confidence level of P ＜0.05. We identified key flavor-related differentially accumulated metabolites using the variable importance in projection (VIP) value (VIP ≥ 1.0) and fold change ≥ 2 or ≤ 0.5. Furthermore, the results of the HPLC analysis showed that white-fleshed loquats had a low malic acid content. We also performed the UPLC-MS/MS system to investigate the carotenoids contents and lipidome in four triploid cultivars. In the fruits of white-fleshed varieties, the carotenoids contents were significantly downregulated, but the contents of most glycerolphospholipids were increased. Our results reveal the metabolomic changes between the yellow- and white-fleshed cultivars.
研究领域:
园艺学
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代谢组学
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植物生物化学
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产品关联
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 5.7
Jiafei Qian,Wenwen You,Yicheng Wang,Yunlin Cao,Xiaoyong Zhao,Hongxia Xu,Hang Ge,Xiaoying Li,Di Wu,Junwei Chen,Xian Li
Loquat ( Eriobotrya japonica ) is rich in flavonols with health-promoting functions. However, regulatory mechanisms of flavonol biosynthesis in loquat are unclear. This study functionally characterized an R2R3-MYB, EjMYBF1 from loquat. Expression level of EjMYBF1 is positively correlated with flavonol content at different stages of fruit development and in ten accessions with differential flavonol accumulation. Dual-luciferase assays and EMSA indicated that EjMYBF1 transactivated the promoters of structural genes involved in flavonol biosynthesis by binding to promoter regions containing MYBCORE elements. Transient overexpression and virus-induced gene silencing assays of EjMYBF1 in loquat fruit peels and leaves, as well as heterologous constitutive expression of EjMYBF1 in Nicotiana tabacum confirmed that EjMYBF1 is a positive regulator of flavonol biosynthesis in loquat. These results demonstrate that EjMYBF1 is a functional flavonol-specific activator in loquat and explain one reason for its high flavonol content from the perspective of transcriptional regulation. This work provides a potential target for improving loquat nutrition and for applications in secondary metabolic engineering.
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PubMed
高影响因子
Materials
·
2019
·Vol. 12
·期 1
·189
·
IF 2.972
Chen Yu,Jingchun Tang,Xiaomei Liu,Xinwei Ren,Meinan Zhen,Lan Wang
This article reports on silver nanoparticles (AgNPs) that were green-synthesized by usingEriobotrya japonica(Thunb.) leaf extract and their use for the catalytic degradation of reactive dyes. The properties of biogenic AgNPs were characterized using UV-vis absorption spectroscopy, field emission scanning electron microscope (FESEM), X-ray powder diffraction (XRD), transmission electron microscope (TEM), Fourier transforming infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDS), and selected area electron diffraction (SAED) analysis. The UV-vis spectroscopy and X-ray analyses confirmed the formation of AgNPs and showed the strong absorbance around 467 nm with surface plasmon resonance (SPR). The mean diameter of biogenic AgNPs at room (20 °C), moderate (50 °C), and high temperatures (80 °C) were 9.26 ± 2.72, 13.09 ± 3.66, and 17.28 ± 5.78 nm, respectively. The reaction temperature had significant impacts on the sizes of synthesized AgNPs. The higher the synthesis temperature, the larger size and the lower catalysis activity for reductive decomposition of reactive dyes via NaBH4. The results supported a bio-green approach for developing AgNPs with a small size and stable degradation activity of reactive dyes over 92% in 30 min by usingEriobotrya japonica(Thunb.) leaf extract at pH 7, 20 °C, and 1:10 ratio of silver nitrate added to the leaf extract.Keywords:green biosynthesis;silver nanoparticles;Eriobotrya japonica;plant extract;catalyst
研究领域:
纳米生物学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Lijuan Dong,Qingbo Jiao,Jian Gao,Xin Luo,Yuling Song,Ting Li,Chen Huan,Min Huang,Gerui Ren,Qing Shen,Linglin Fu,Hujun Xie,Zisheng Luo
The loquat ( Eriobotrya japonica ) has high nutritional and economic values. However, loquat easily rots at room temperature, resulting in the loss of commodity value. In this study, zein, lecithin, and (−)-epigallocatechin gallate (EGCG) were used to prepare Zein-Lecithin-EGCG (Z-L/E) nanoparticles, and the effects of these nanoparticles on loquat quality were studied. EGCG addition affected the zeta potential values of the nanoparticles, which increased from −11.80 mV to −23.34 mV. The Fourier transform infrared (FTIR) results showed that electrostatic interactions and hydrogen bonding formed among lecithin, zein, and EGCG. The Z-L/E nanoparticles showed great antioxidant activity with the DPPH radical scavenging rate of 84.76% and the ABTS radical scavenging rate of 80.95% for Z-L/E 4:1 sample, and had good antibacterial ability by decreasing the growth rate of E. coli . The coating treatment with Z-L/E nanoparticles for loquats reduced the weight loss and the decay rate by 13.3% and 1.4%, respectively, during the storage at 25 °C for 22 days, and the decrease of titratable acidity (TA) and vitamin C (VC) content in loquats were also effectively delayed. The coating treatment with Z-L/E nanoparticles for loquats could significantly prolong the storage life of fruits and maintain the quality of fruits.
研究领域:
食品科学
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采后技术
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纳米技术
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植物科学
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产品关联
DOI
高影响因子
Scientific Reports
·
2019
·Vol. 9
·期 1
·1-13
·
IF 4.011
Xu Shuwen,Wang Gang,Peng Wei,Xu Yandi,Zhang Yu,Ge Ying,Jing Yue,Gong Zhunan
Type 2 diabetes (T2D) with high morbidity and mortality is characterized by abnormal glucose and lipid metabolism due in part to insulin resistance in liver, which lead to elevated hyperglycemia and hyperlipidemia. This study sough to explore the effects of corosolic acid (CA) in different T2D models and explored the underlying mechanism. Separated from Eriobotrya japonica leaves, CA purity was above 95% measured by a HPLC method. Compared with cAMP and DEX induced T2D HepG2 model, CA significantly stimulated glucose consumption and improved glycogen accumulation by inhibiting PEPCK mRNA expression. And in cAMP and DEX induced T2D zebrafish model, CA reduced glycogen degradation and increased glucose consumption by regulating some key enzymes in carbon metabolism including GLUT1, GLUT2, GLUT3, LDHA, LDHB, GP, G6Pase, GYS1, and PFKFB3. In addition, insulin receptor signals were also involved in CA-regulated hypoglycemic action. Furthermore, in STZ-induced T2D rat model, compared with diabetic control groups, CA remarkably downregulated the levels of serum lipid, blood glucose, ICAM-1, malonaldehyde and insulin resistance index, while upregulated SOD activity and impaired glucose tolerance. In a conclusion, CA can regulate glucose and lipid metabolic adaptation in T2D like HepG2, zebrafish and rat models partly through reducing inflammation and oxidative stress and suppressing PEPCK .
研究领域:
药理学
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糖尿病研究
·
分子生物学
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DOI
PubMed
高影响因子
Journal of Future Foods
·
2026
·
IF 7.2
Rui Wang,Pei-Xing Li,Cui-Wen Deng,Yi Chen,Yuan-Yi Mo,Yu-Wei Wu,Qi-Qing Liang,Ai Xu,Ben Yu,Lang-Hong Wang,Xin-An Zeng
Loquat fruit is highly susceptible to chilling injury during low-temperature storage, leading to tissue browning and quality deterioration, which severely limits its postharvest commercial value. This study investigated the efficacy of pulsed electric field (PEF) pretreatment in maintaining the quality of cold-stored loquat fruit at 1°C. The results showed that PEF pretreatment exerted dose-dependent effects on fruit quality preservation. Treatment at 0.3 kV/cm effectively suppressed browning and reduced the decay rate to 23.20 ± 2.44% after 16 days of storage, significantly lower than that of the control (46.93 ± 1.14%). Additionally, this treatment better maintained fruit firmness, minimized weight loss, and preserved higher vitamin C content, thereby improving both visual and nutritional quality. However, quality declined at a higher PEF intensity of 0.5 kV/cm, likely due to excessive electroporation-induced cellular damage. During early storage, the 0.3 kV/cm treatment increased total phenolic content by 72.36% and sustained higher total flavonoid levels (0.84–0.99 mg/g) throughout storage. Concurrently, polyphenol oxidase (PPO) activity decreased to 33.94 ± 0.66 U/g by day 16, while peroxidase (POD) activity remained consistently lower than in the control. The elevated accumulation of antioxidant compounds, coupled with the inhibition of key browning-related enzymes, appears to be the primary mechanism by which PEF alleviates chilling-induced browning. In conclusion, PEF pretreatment offers an effective strategy for extending the shelf life of cold-stored loquat fruit and shows potential for application in other chilling-sensitive fruits.
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DOI
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 5.7
Jiafei Qian,Chuanhong Ren,Fan Wang,Yunlin Cao,Yan Guo,Xiaoyong Zhao,Yilong Liu,Changqing Zhu,Xiaoying Li,Hongxia Xu,Junwei Chen,Kunsong Chen,Xian Li
Flavonol glycosides are secondary metabolites important for plant development and stress defense such as UV-B irradiation. UDP-glycosyltransferase (UGT) is the last enzyme step that catalyzes flavonol glycosides biosynthesis. Eriobotrya japonica is abundant in flavonol glycosides, but UGTs responsible for accumulation of flavonol glycosides remain unknown. Here, 13 flavonol glycosides including monoglycosides and diglycosides were characterized in different tissues of loquat by LC-MS/MS. UV-B irradiation significantly increased the accumulation of four quercetin glycosides and two kaempferol glycosides in loquat fruit. Based on UGT gene family analysis, transcriptome analysis, enzyme assays of recombinant proteins as well as transient overexpression assays in Nicotiana benthamiana , three UGTs were identified, i.e. EjUGT78T4 as flavonol 3- O -galactosyltransferase, EjUGT78S3 as flavonol 3- O -glucosyltransferase, and EjUGT91AK7 as flavonol 1→6 rhamnosyltransferase. This work elucidates the formation of flavonol glycosides in loquat through UGT-mediated glycosylation.
研究领域:
植物学
·
分子生物学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Chenlei Ma,Honglei Bao,Beibei Zhu,Junchao Zhu,Songlin Chen,Huawei Lv,Chu Chu,Shengqiang Tong
Micro-fraction bioactivity profiling and high speed countercurrent chromatography were performed for screening and isolation of components with α -glucosidase inhibitory activity from Eriobotrya japonica (Thunb.) leaves. Three sesquiterpene glycosides and a number of known pentacyclic triterpene acids, including a new compound, were successfully screened. Sesquiterpene glycosides were found to have α -glucosidase inhibitory activity for the first time. An efficient strategy for preparative separation of the three sesquiterpene glycosides from E. japonica leaves by column chromatography combined with two-step high speed countercurrent chromatographic separation was established. Two biphasic solvent systems, including n-hexane-ethyl acetate-methanol-water (5:95:5:95, v/v) and ethyl acetate-ethanol-water (100:20:80, v/v), were selected. In the first countercurrent chromatographic separation, 17.50 mg of sesquiterpene glycoside 1 with 92.9% purity and 15.11 mg of sesquiterpene glycoside 3 with 94.4% purity were isolated from 80.10 mg of partially purified fraction I, and in the second separation, 4.42 mg of new sesquiterpene glycoside 2 with 94.9% purity were isolated. Each screened compound was evaluated by α -glucosidase inhibition assay, and results showed that the new sesquiterpene glycoside 2 had high inhibitory activity with IC 50 = 7.83±0.01 μ M.
研究领域:
植物化学
·
药物化学
·
生物活性研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Honglei Bao,Wenyu Sun,Yang Jin,Chaoyue Wang,Xiang Wang,Hengmian Sun,Chu Chu,Shengqiang Tong
Maslinic acid and corosolic acid with high purity were successfully separated from Eriobotrya japonica (Thunb.) leaves by two-step countercurrent chromatographic separation. Two biphasic solvent systems composed of petroleum ether-ethyl acetate-ethanol-water (6:4:5:5, v/v) and petroleum ether-ethyl acetate-ethanol-0.10 mol/L of hydroxypropyl- β -cyclodextrin with pH 7.0 (8:2:3.5:6.5, v/v) were selected according to the partition performance of the main structural isomeric pentacyclic triterpenes. The influences of pH value and concentration of hydroxypropyl- β -cyclodextrin in separation of two isomers were investigated. In first step countercurrent chromatographic separation, a mixture of two target structural isomers (14.12 mg of sample I) was separated from 40.00 mg of a partially purified sample. In second step countercurrent chromatographic separation, maslinic acid and corosolic acid were completely isolated from 12.00 mg of sample I with hydroxypropyl- β -cyclodextrin as aqueous phase additive. The recoveries of the two isomers were over 90%, yielding 5.18 mg of maslinic acid and 5.47 mg of corosolic acid, respectively.
研究领域:
植物化学
·
分离科学
·
天然产物研究
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）