COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2022
·
IF 4.52
Rui Zhang,Zhigang Zhao,Mingshuai Li,Liang Luo,Shihui Wang,Kun Guo,Wei Xu
Aquatic water with carbonate alkalinity presents a survival challenge to aquatic animals. As an economically important crab, large quantities of Eriocheir sinensis are cultured in carbonate-type saline-alkali ponds, while the toxic effect on E. sinensis from carbonate alkalinity is still unclear. In this study, untargeted liquid chromatography-mass spectrometry metabolomics was performed to investigate the metabolic change caused by culture alkalinity, and confirmed distinct physiological response under gradient alkalinities. There were 39 differential metabolites obtained in the low-alkalinity group (4.35 mmol/L) versus control group, and “arachidonic acid metabolism” was enriched as a core response pathway. 93 differential metabolites were identified in the high-alkalinity group (17.43 mmol/L) versus control group, and a complex response net was manifested through integrated analysis, building by “steroid hormone biosynthesis”, “phenylalanine, tyrosine and tryptophan biosynthesis”, “phosphonate and phosphinate metabolism”, “phenylalanine metabolism”, “mineral absorption”, “purine metabolism” and “carbon metabolism”. This indicated the mobilization of energy reserves and the suppression of protein and amino acid catabolism were manifested in E. sinensis gills to defense high alkalinity stress. In addition, the persistently regulation of key metabolites under various alkalinity, including diuretic compound “spironolactone” and the antiphlogistic compound “LXB 4 ”, suggested anti-inflammatory action and excretion regulation were initiated to defend the stress.
研究领域:
水产养殖学
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环境毒理学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Zhigang Yang,Liangliang Zhu,Jiani Liu,Yongxu Cheng,Khor Waiho,Aqin Chen,Youji Wang
Microplastics may be potential vectors for environmental contaminants such as heavy metals in the aquatic ecosystem due to their highly hydrophobic surfaces and fugacity property. To investigate the combined effects of microplastics with Pb, we exposed juvenile Chinese mitten crabs Eriocheir sinensis to different Pb concentrations (0, 5 and 50 μg/L) combined with microplastics (0 and 400 μg/L) for 21 days to determine the Pb bioaccumulation, oxidative stress, lipid anabolism, and histopathology of hepatopancreas. In general, the results showed that compared to single Pb exposure, the combination of MPs and Pb significantly increased the bioaccumulation of Pb, activities/content of antioxidant biomarkers and lipid metabolism enzymes, and liver injury parameters in crabs, indicating MPs are potential vector of heavy metals and co-exposure exerts more severe effects on crabs. This study provides the insights into the oxidative defense and preliminary lipid anabolism of economic crustaceans in response to combined stress of Pb and MPs.
研究领域:
环境科学
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水生毒理学
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生态毒理学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Shunli Che,Mengting Huang,Lemei Zhu,Longteng Shen,Yuan Ma,Zhicheng Wan,Xuguang Li,Jun Zhou,Shuquan Ding,Xilei Li
Nanopolystyrene (NP) and phoxim (PHO) are common environmental pollutants in aquatic systems. We evaluated the toxic effects of exposure to ambient concentrations of NP and/or PHO in the intestines of the Chinese mitten crab ( Eriocheir sinensis ). Our study showed that histopathological changes were observed in the intestines. Specifically, NP and/or PHO exposure increased intraepithelial lymphocytes. Furthermore, NP and/or PHO exposure induced oxidative stress, as evidenced by a significant decrease in superoxide dismutase activity (SOD), peroxidase activity (POD), and total antioxidant capacity (T-AOC). Pro-inflammatory gene expression and transcriptome analysis demonstrated that NP and/or PHO exposure induced the intestinal inflammatory response. Transcriptome results showed that NP and/or PHO exposure upregulated the NF-κB signaling pathway, which is considered a key pathway in the inflammatory response. Additionally, the expression of pro-inflammatory genes significantly increased after a single exposure to NP or PHO, but it exhibited a significant decrease after the co-exposure. The downregulation of these genes in the co-exposure group likely suggested that the co-exposure mitigated intestinal inflammation response in E. sinensis . Collectively, our findings mainly showed that NP and/or PHO exposure at ambient concentrations induces oxidative stress and inflammatory response in the intestines of E. sinensis .
研究领域:
环境毒理学
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水生生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2022
·Vol. 2022
·期 1
·8348000
·
IF 3.781
Xuran Liu,Wenlong Wan,Mengge Li,Jiayuan Shi,Jie Xu,Zihan Zhou,Anran Wang,Shuyan Miao
Effects of dietary niacin on the growth performance, intestinal histomorphology, body composition, and antioxidant capacity were investigated in the present study to determine the optimum requirement of niacin for juvenile Eriocheir sinensis . All 360 crabs (initial average weight 1.14 ± 0.04 g) were randomly divided into 6 groups with 3 replicates in each group and 20 crabs in each replicate. Crabs were fed with the control diet (0.89 mg/kg) or niacin-supplemented diets (170.54 mg/kg, 347.05 mg/kg, 587.59 mg/kg, 784.85 mg/kg, and 1248.86 mg/kg) for 12 weeks (named as G1, G2, G3, G4, G5, and G6, respectively). The results showed that appropriate dietary niacin (above 347.05 mg/kg) significantly increased the weight gain rate (WGR) and specific growth rate (SGR) ( p < 0.05), but did not affect the survival rate (SR), feed conversion ratio (FCR), daily feeding rate (DFR), and molting frequency (MF) of crabs ( p > 0.05). The niacin content in the hepatopancreas of crabs in G1 and G2 was significantly lower than that of the other four groups ( p < 0.05). Moreover, dietary niacin significantly affected the intestinal histomorphology of crabs, including the number of folds (NF), height of folds (HF), height of microvillus (HMV), and thickness of muscularis (TM) ( p < 0.05). Additionally, moderate dietary niacin levels significantly affected the nonspecific immune responses of crabs, by improving the activity of catalase (CAT), glutathione s-transferase (GST), glutathione peroxidase (GSH-Px), and total superoxide dismutase (T-SOD) ( p < 0.05). Based on the broken-line model analysis of SGR against dietary niacin level, the dietary niacin requirement of juvenile crabs was suggested to be 419.4 mg/kg.
研究领域:
水产养殖学
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营养学
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DOI
高影响因子
Food Chemistry-X
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2025
·
IF 8.2
Tianyu Wang,Hang Lu,Yuanyong Tian,Hui Zhao,Xuefeng Lu,Zhaoxia Wu,Shiwei Niu
Ammonia accumulation in aquaculture systems affects the sensory quality of Eriocheir sinensis , and mechanisms linking ammonia to aroma changes remain unclear. This study investigated how ammonia exposure in aquatic environments (0, 10.47, and 41.87 mg/L total ammonia-N, 48 h) alters aroma profiles of E. sinensis meat. Using HS-SPME-GC–MS and HS-GC-IMS, we identified 26 key volatile organic compounds (VOCs) defining aroma differences. Concurrently, fatty acid profiles were analyzed through GC–MS. Ammonia exposure reduced fishy (trimethylamine, 1-octen-3-ol) and meaty (methional) aromas while enhancing plant-like (phenylethyl alcohol) and nutty (2-methylbutanal) aromas in the low-concentration group; high-concentration ammonia diminished hexanal and sulfur-containing compounds, aligning with aroma evaluation trends. Further, n-3 polyunsaturated fatty acids increased after ammonia exposure, correlating with VOCs derived from lipid metabolism. These findings reveal ammonia-induced metabolic changes in flavor precursors (e.g., fatty acids) and flavor compounds (e.g., VOCs) and provide feasible insights for optimizing aquaculture practices to improve crab quality.
研究领域:
水产养殖
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食品科学
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分析化学
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环境毒理学
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风味化学
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DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2024
·
IF 3.9
Mengting Huang,Yuan Ma,Qianru Fan,Shunli Che,Jun Zhang,Shuquan Ding,Shuren Zhu,Xilei Li
Nanopolystyrene (NP) and phoxim (PHO) are pervasive environmental contaminants that pose a significant threat to the health of aquatic organisms, prompting widespread concern among researchers and the public alike. The hepatopancreas play important roles in the Chinese mitten crab ( Eriocheir sinensis ), such as digestion, absorption and detoxification. This study assessed the hepatopancreatic toxicity caused by the exposure of Eriocheir sinensis to environmentally relevant concentrations of NP and/or PHO. After a 21-day exposure period, NP (1.0 × 10 10 particles/L) and PHO (24 μg/L) exposure resulted in reduced number of blister-like, resorptive, and fibrillar cells and an elevation in lipid droplets within the hepatopancreas compared to the control group. Furthermore, trypsin and lipase activity decreased, amylase activity increased, and a significantly decrease in the expression of digestion-related genes, including CHT , CarL , and CarB , suggested impairment in both digestive and metabolic functions. The marked upregulation of key genes, including PPARγ , GYK , PEPCK , and SCD , as well as key metabolites such as 4-methylzymosterol-carboxylate, zymosterone, lathosterol, 7-dehydro-desmosterol, vitamin D2, 24-methylene-cycloartanol, 5-dehydroepisterol, and sitosterol in the lipid metabolic pathway, showed that the peroxisome proliferator-activated receptor (PPAR) and steroid biosynthesis signaling pathways were highly affected by exposure to NP and/or PHO. These findings indicated that exposure to NP and/or PHO might adversely affect the hepatopancreatic physiological homeostasis in E. sinensis by causing tissue damage and interfering with digestive and metabolic functions. Our results provide ecotoxicological insights into the effects of nanopolystyrene and/or phoxim exposure on the digestive function of Eriocheir sinensis .
研究领域:
环境科学
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生态毒理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2026
·
IF 3.9
Chen Mengmeng,Cao Huan,Wang Zijun,Pan Yingjie,Zhao Yong,Liu Haiquan
This study investigated the effects of six cooking methods (steaming, bag-steaming, microwaving, bag-microwaving, boiling, and frying) on arsenic species in Eriocheir sinensis , and assessed bioaccessibility and health risk for raw, microwaved, and steamed samples. Bag-steaming increased pentavalent inorganic arsenic (iAs 5 ⁺) in female breast meat by 283.13%. Boiling elevated iAs 5 ⁺ in male hepatopancreas by 431.06%. Steaming decreased trivalent inorganic arsenic (iAs 3 ⁺) in male hepatopancreas by 90.39%, whereas microwaving reduced it in female hepatopancreas by 91.04%. Gastrointestinal bioaccessibility of iAs 5+ was enhanced under microwaving (202.90% in males and 173.91% in females), indicating matrix-dependent release. Target cancer risk varied among cooking methods, with microwaving exceeding 10 –6 in some cases. Cooking induced pronounced arsenic heterogeneity, as divergent responses across tissues and sexes suggested species retention in edible tissues or release into exudates. These findings indicate that cooking-induced speciation changes and bioaccessibility may influence dietary exposure, providing a scientific basis for dietary safety recommendations.
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DOI
高影响因子
AQUATIC TOXICOLOGY
·
2024
·
IF 4.1
Mengting Huang,Yuan Ma,Shunli Che,Longteng Shen,Zhicheng Wan,Shiping Su,Shuquan Ding,Xilei Li
Significant concerns have been raised by the widespread pollutants phoxim (PHO) and nanopolystyrene (NP) in the natural environment. This study evaluated the toxicity effects on the hepatopancreas of Eriocheir sinensis caused by NP and/or PHO at concentrations found in the environment. Subchronic exposure to NP and/or PHO triggered hepatopancreas histological damage within a 21-day exposure period. The NP, PHO, and co-exposure (NPO) groups exhibited fewer blister-like (B) cells, along with the appearance of vacuolation. Furthermore, these exposures induced impairment in the hepatic tubule mucus barrier and mechanical barrier, as evidenced by altered expression of oxidative stress-related genes, mucin-related genes, and TJ-related genes. Additionally, alterations in immunity-related genes and inflammatory cytokine genes expression were observed. The findings showed that hepatopancreas inflammation was caused by both individual and combined exposure to NP and PHO and that the inflammatory response was exacerbated by the co-exposure. The possible pathways of hepatopancreas toxicity were further investigated by transcriptomic analysis. Hepatopancreas inflammation was brought on by subchronic exposure to PHO and co-exposure; this inflammation was exacerbated by co-exposure and was backed by the activation of NF-κB signaling pathway via targeting-related genes. In summary, this research represents the initial documentation, to the best of our understanding of the detrimental effects of exposured to NP and/or PHO at levels found in the environment disrupt the hepatopancreas mucus and mechanical barrier in crustaceans, triggering inflammatory responses. These findings highlight the significance of NP and/or PHO pollution for hepatopancreas health.
研究领域:
环境科学
·
毒理学
·
水生生物学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Dan-Dan Bian,Jun-Jie Jiang,Shuang-Gui Dai,Wen-Hui Tang,Yaohui Chen,Kaiwen Shi,Qiu-Ning Liu,Bo-Ping Tang
Tricyclazole (TRCZ), a widely used fungicide in rice-crab coculture, poses a potential risk to the Chinese mitten crab (Eriocheir sinensis). This study aimed to elucidate its hepatopancreatic toxicity mechanism by integrating physiological, biochemical, and transcriptomic analyses. We determined a 96 h LC50 of 54.28 mg/L and a safe concentration of 22.09 mg/L. The effects of subchronic (28 days) and acute (96 h) exposure were investigated in the E. sinensis. TRCZ exposure induced oxidative stress, immune dysregulation, metabolic disruption, and apoptosis, leading to histopathological damage in the hepatopancreas of E. sinensis. Transcriptomic profiling revealed 4809 differentially expressed genes, with the MAPK signaling pathway being significantly enriched. RNA interference targeting Ras confirmed the pivotal role of the Ras-MAPK cascade in mediating TRCZ-induced injury. Our findings reveal the mechanism of TRCZ toxicity and provide an insight for its risk assessment and safe application in rice-crab coculture systems.
研究领域:
生态毒理学
·
水生毒理学
·
分子生物学
·
环境科学
·
转录组学
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DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2024
·
IF 3
Xiaorong Huang,Ru Tan,Zhi Geng,Tao Zhang,Guangpeng Feng,Gang Yang,Feng Zhao,Ping Zhuang
A 60-day feeding trial was conducted to evaluate the impact of dietary Antarctic krill meal on the reproductive performance and embryo quality of the Chinese mitten crab, Eriocheir sinensis. Three diets were formulated, incorporating varying levels of Antarctic krill meal at 0% (Diet K0), 10% (Diet K10), and 20% (Diet K20), with a control group fed razor clam Sinonovacula constricta. Each diet was randomly assigned to three replicate tanks, each stocked with 5 males and 10 females. Male and female weights were 145.38 ± 8.01 and 102.57 ± 9.73 g, respectively. The results revealed no significant differences in weight gain rate, specific growth rate, and survival rate. However, the hepatopancreatic weight and hepatopancreas index of female crabs in each group decreased, while gonadal weight and gonadosomatic index increased significantly after 60 days, with Diet K20 showing the highest values. Egg production and fecundity of female crabs reached their peak in Diet K20, with no significant differences in reproductive indices among all groups. The phospholipid content in Diet K20 was significantly higher than in the other groups (). Cholesterol contents in Diet K0 and the control group were significantly higher than in Diet K10 and K20 (). No significant differences were observed in egg diameter, egg weight, moisture, crude protein, and crude fat between the groups. The content of C20 : 2 and C20 : 4n6 was highest in Diet K0, with a significant difference compared to Diet K10 (). However, no significant differences were found in the total content of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids among all groups. Based on the research findings, it is recommended that the optimal level of Antarctic krill meal in diets is 20%.
研究领域:
水产养殖学
·
动物营养学
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产品关联
DOI
PubMed
高影响因子
Aquaculture Reports
·
2026
·
IF 3.8
Yuan Ma,Yao Li,Wei Wu,Kaixin Ren,Mengting Huang,Beijie Shen,Hu Yu,Zhicheng Wan,Shuquan Ding,Xilei Li
Fermented feed is widely used in aquaculture for its roles in promoting animal growth, improving water quality, and meeting nutritional requirements. The hepatopancreas is crucial for digestion and absorption of Chinese mitten crab, Eriocheir sinensis . This study evaluated the effects of different proportions of fermented feed (0%, 10%, 20%, 30% and 40%) on the growth performance and digestion of E. sinensis following a 60-day feeding trial. Growth performance, digestive enzyme activities, and expression of digestion-related genes peaked in the 30% fermented feed group, with concurrent increases in hepatopancreatic blister-like and fibrillar cells. Further analyses of hepatopancreas microbiota and metabolomics (0% and 30% groups) revealed that 30% fermented feed maintained microbial community homeostasis and upregulated key metabolic pathways, including aminoacyl-tRNA biosynthesis, biosynthesis of amino acids, and tryptophan metabolism. Spearman correlation analysis revealed that several key differentially expressed metabolites were positively correlated with the presence of major bacterial genera following feeding with 30% fermented feed. These findings indicate that the intake of fermented feed may benefit hepatopancreatic physiological homeostasis in juvenile E. sinensis by enhancing growth performance and digestive and metabolic functions, and 30% fermented feed had the best effect by regulating hepatopancreatic microbial communities and metabolic pathways.
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 5
·4412
·
IF 5.6
Xinping Guo,Ziang Qian,Qiqi Pan,Yuqing Hu,Wangxin Mei,Xiumei Xing,Shaowu Yin,Jie Ji,Kai Zhang
Excessive use of antibiotics in aquaculture causes residues in aquatic animal products and harms human health. However, knowledge of florfenicol (FF) toxicology on gut health and microbiota and their resulting relationships in economic freshwater crustaceans is scarce. Here, we first investigated the influence of FF on the intestinal health of Chinese mitten crabs, and then explored the role of bacterial community in FF-induced intestinal antioxidation system and intestinal homeostasis dysbiosis. A total of 120 male crabs (48.5 ± 4.5 g) were experimentally treated in four different concentrations of FF (0, 0.5, 5 and 50 μg/L) for 14 days. Responses of antioxidant defenses and changes of gut microbiota were assessed in the intestine. Results revealed that FF exposure induced significant histological morphology variation. FF exposure also enhanced immune and apoptosis characteristics in the intestine after 7 days. Moreover, antioxidant enzyme catalase activities showed a similar pattern. The intestinal microbiota community was analyzed based on full-length 16S rRNA sequencing. Only the high concentration group showed a marked decrease in microbial diversity and change in its composition after 14 days of exposure. Relative abundance of beneficial genera increased on day 14. These findings illustrate that exposure to FF could cause intestinal dysfunction and gut microbiota dysbiosis in Chinese mitten crabs, which provides new insights into the relationship between gut health and gut microbiota in invertebrates following exposure to persistent antibiotics pollutants.Keywords:florfenicol;gut barrier;apoptosis;gut microbiota;crab
研究领域:
水产养殖
·
毒理学
·
微生物学
·
动物健康
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）