MICROCHIMICA ACTA
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2013
·Vol. 181
·期 1
·155-162
·
IF 3.434
Wang Liheng,Liao Xiaolei,Ding Yingtao,Gao Fei,Wang Qingxiang
Abstract We report on an electrochemical DNA biosensor consisting of a glassy carbon electrode modified with a film of electropolymerized Eriochrome Black T (pEBT) that serves as a functional platform for the immobilization of probe DNA. pEBT was deposited via cyclic voltammetry, and the amino-modified DNA capture probe was covalently linked to the surface via a sulfanilamide coupling reaction. The single step of the assembly process was monitored by atomic force microscopy and electrochemistry. The surface density of DNA probe on the biosensor interface was calculated to be 1.7 × 10 −10 mol cm −2 using methylene blue as an electroactive probe. Hybridization experiments showed the peak currents of methylene blue to decrease with increasing concentration of complementary sequence in the range from 5.0 f. to 5.0 pM. The detection limit is as low as 0.11 fM. Selectivity studies showed that the biosensor can discriminate a fully complementary sequence from a single-base mismatch, three-base mismatch, and a fully non-complementary sequence. The biosensor displays good stability and can be regenerated due to the beneficial effects of electropolymerization and covalent immobilization of probe DNA.
A novel DNA biosensor was fabricated through a facile sulfamide coupling reaction between probe DNA and eriochrome black T that electropolymerized on a glassy carbon electrode
研究领域:
生物化学
·
分子生物学
·
分析化学
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2019
·
IF 3.131
Xia Li,Xiao-Juan Nie,Yu-Nuo Zhu,Wen-Chao Ye,Yu-Lin Jiang,Shi-Long Su,Bi-Ting Yan
In this paper, a new β-cyclodextrin polymer (β-CDBEP) was successfully prepared by derivatizing with 1H-benzotriazole followed by cross-linking with epichlorohydrin . The adsorption behaviour of β-CDBEP for anionic Eriochrome Black T (EBT) was studied by batch experiments. The results showed that the highest adsorption capacity was obtained in acidic medium (pH = 5), and the pseudo-second-order model fitted well with the experimental data indicating the rate-determining step mainly determined by chemisorption . The adsorption equilibrium data followed better the Freundlich isotherm model with multi-layered adsorption compared to the Langmuir isotherm model. Thermodynamic parameters indicated that the adsorption of EBT onto β-CDBEP was an endothermic and spontaneous process, and a favorable adsorption happened at a relatively higher temperature. The combined electrostatic interactions , inclusion complexation, π-π interactions and intra-particle diffusion contributed to the adsorption of EBT onto β-CDBEP.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Zi-Han Cheng,Xun Liu,Shang-Qing Zhang,Ting Yang,Ming-Li Chen,Jian-Hua Wang
The timely warning of the germination of bacterial spores and their prevention are highly important to minimize their potential detrimental effects and for disease control. Thus, a sensitive and selective assay of biomarkers is most desirable. In this work, a nanoprobe is constructed by conjugating lanthanide upconversion nanoparticles (UCNPs) with sodium tripolyphosphate (TPP) and eriochrome black T (EBT). The nanoprobe, UCNPs−TPP/EBT, serves as a platform for the detection of the anthrax biomarker, dipicolinic acid (DPA). In principle, DPA displaces EBT from the UCNPs−TPP/EBT nanoconjugate, resulting in a color change from magenta to blue because of the release of free EBT into the aqueous solution. The binding sites on UCNPs are partly preblocked with TPP as the placeholder molecule, leaving a desired number of binding sites for EBT conjugation. On the basis of this dye displacement reaction, a novel colorimetric assay protocol for DPA is developed, deriving a linear calibration range from 2 to 200 μM with a detection limit of 0.9 μM, which is well below the infectious dose of the spores (60 μM). The assay platform exhibits excellent anti-interference capability when treating a real biological sample matrix. The present method is validated by the analysis of DPA in human serum, and its practical application is further demonstrated by monitoring the DPA release upon spore germination.
研究领域:
生物化学
·
分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Shan Lu,Xuelin Dong,Binbin Zhang,Qi Zhou,Xinwei Zhang,Yonggui Liao,Yajiang Yang,Hong Wang
In this work, a dual-channel probe based on a complex of Eriochrome Black T (EBT) and Eu 3+ ions (denoted as EBT/Eu 3+ ) was used to sense four kinds of tetracyclines (TCs). UV–Vis and fluorescence spectra indicated that EBT/Eu 3+ molecule has the ability to emit dual channel signals of color and fluorescence. When TCs with different structures were added to the EBT/Eu 3+ solution, the color and the fluorescence intensities of EBT/Eu 3+ solution varied with TCs structure. FT-IR spectra revealed that a coordinating reaction occurred between acylamino groups, hydroxyl groups of A-ring in TCs and Eu 3+ ions. The coordinating ratio was 1: 2. Utilizing dual channel signals as well as the aid of principal component analysis (PCA), EBT/Eu 3+ was capable of discriminating not only single TCs with concentrations in nano-molar level, but also binary mixtures of TCs. The results of controlled experiments on various interfering substances and other antibiotics proved that this method has high selectivity for TCs detection. Furthermore, this two-channel sensor array based on EBT/Eu 3+ can also be used for the discrimination of unknown TCs in commercially available pork and honey with the 100% recognition accuracy. These results indicated that TCs with different structures can be rapidly discriminated by using a single and easily prepared sensor array.
研究领域:
分析化学
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传感器
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环境科学
·
食品安全
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DOI
高影响因子
BIOELECTROCHEMISTRY
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2012
·
IF 3.759
Xiao Liu,Liqiang Luo,Yaping Ding,Zhangping Kang,Daixin Ye
A novel Au-nanoparticles/poly-eriochrome black T film modified glassy carbon electrode (AuNPs/PEBT/GCE) was constructed for the simultaneous determination of l -cysteine (L-Cys) and l -tyrosine (L-Tyr) by differential pulse voltammetry . Fourier transform infrared spectra and electrochemical impedance spectroscopy indicate that the PEBT film was successfully polymerized on the surface of GCE and the film efficiently decreased the charge transfer resistance value of electrode and improved the electron transfer kinetic between analytes and electrode. The scanning electron microscope image shows that the immobilized AuNPs were spherical in shape and enhanced the electrical conductivity of PEBT film. In addition, PEBT film increased the oxidation currents of analytes four times when compared to bare GCE, and the AuNPs separated the oxidation potentials of L-Cys and L-Tyr by 488 mV while bare GCE failed to resolve them. The amperometry results exhibit that the electrocatalytic currents increased linearly with L-Cys concentrations in the range 0.05–100 μM ( r = 0.9981), and the detection limits of L-Cys and L-Tyr were 8 nM and 10 nM ( S / N = 3), respectively. With high sensitivity and selectivity, the proposed electrochemical sensor provides a simple method for simultaneous determination of L-Cys and L-Tyr.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 23
·5730
·
IF 4.2
Zefeng Jiang,Tongyang Song,Bowen Huang,Chengqiang Qi,Zifu Peng,Tong Wang,Yuliang Li,Linjing Ye
Platanus officinalis fibers (PFs) taking advantage of high-availability, eco-friendly and low-cost characteristics have attracted significant focus in the field of biomaterial application. Polyethyleneimine grafted with polydopamine on magnetic Platanus officinalis fibers (PEI-PDA@M-PFs) were prepared through a two-step process of mussel inspiration and the Michael addition reaction, which can work as an effective multifunctional biomass adsorbent for anionic dye with outstanding separation capacity and efficiency. The as-prepared PEI-PDA@M-PFs possess desirable hydrophilicity, magnetism and positive charge, along with abundant amino functional groups on the surface, facilitating efficient adsorption and the removal of Eriochrome Black T (EBT) dyes from water. In addition to the formation mechanism, the adsorption properties, including adsorption isotherms, kinetics, and the reusability of the absorbent, were studied intensively. The as-prepared PEI-PDA@M-PFs achieved a theoretical maximum adsorption capacity of 166.11 mg/g under optimal conditions (pH 7.0), with 10 mg of the adsorbent introduced into the EBT solution. The pseudo-second-order kinetic and Langmuir models were well matched with experimental data. Moreover, thermodynamic data ΔH > 0 revealed homogeneous chemical adsorption with a heat-absorption reaction. The adsorbent remained at high stability and recyclability even after five cycles of EBT adsorption processes. These above findings provide new insights into the adsorption processes and the development of biologic material for sustainable applications.
研究领域:
环境科学
·
材料科学
·
生物工程
✓ 已验证
DOI
PubMed
高影响因子
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