JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2017
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IF 2.603
Xiao Zhao,Youfa Xu,Lifang Zhang,Chunmei Wang,Chunna Guo,Chenzhong Fei,Keyu Zhang,Xiaoyang Wang,Yingchun Liu,Mi Wang,Wenli Zheng,Feiqun Xue
Ethanamizuril (EZL) is a novel anti-coccidial triazine synthesized by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences. As a novel coccidiostat, it is essential to study its residue in food animal tissues. In this study, a reliable UPLC method for the determination of ethanamizuril (EZL) and one of its metabolites (M3) in chicken muscle, skin and fat, liver, and kidney samples was developed. Analytes were extracted using acetonitrile and 10% sodium carbonate solution, defatted by n -hexane, and further purified by using an Oasis MCX cartridge. The calibration curves for both test compounds were linear in all tissue matrices we tested: muscle, skin and fat, liver and kidney. The mean relative recoveries of EZL and M3 were from 87.3% to 103.2% between all tissue samples. The inter-day and intra-day relative standard deviations were within 15% and 20%, respectively. This method is accurate, reproducible and is ready to be field-tested.
研究领域:
兽医学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
VETERINARY PARASITOLOGY
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2020
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IF 2.157
Yikai Fu,Junwen Zhou,Lifang Zhang,Chenzhong Fei,Xiaoyang Wang,Mi Wang,Chunmei Wang,Keyu Zhang,Feiqun Xue,Yingchun Liu
The treatment effect of ethanamizuril (EZL) to broiler chickens experimentally infected with 8 × 10 4 Eimeria tenella was evaluated. On the third day after infection, the broiler chickens were treated with EZL by gavage at doses of 2, 4, and 8 mg/kg body weight (bw) for once. For double administration, the challenged broiler chickens were administered EZL at doses of 1, 2, 4, and 8 mg/kg bw by gavage continually on the third day and fourth day and once a day. Throughout the experimental period, performance parameters including body weight gain, mortality, cecal lesion score, bloody diarrhoea and oocyst output were recorded. The anticoccidial efficacy was evaluated using the anticoccidial index (ACI). Meanwhile, the concentrations of EZL in chicken cecal contents were measured, and the data were analyzed with a non-compartmental model. The results indicated that EZL showed good anticoccidial activity at single dose of 4 mg/kgbw, with the corresponding ACI of 175.73. When the challenged chickens were treated with EZL under double administration, the EZL showed a medium level of anticoccidial activity at a dose of 2 mg/kg bw, with the corresponding ACI of 162.48. The maximum concentrations (C max ) of EZL in content were 2.43 ± 1.16, 4.28 ± 1.56, and 8.57 ± 1.33 mg/kg after the chickens were administrated at doses of 2, 4, and 8 mg/kg bw, respectively. The respective areas under the curve were 36.93 ± 8.91, 96 ± 16.31, and 262.76 ± 51.52 mg/kg h. The respective half-lives (T 1/2 ) were 10.82 ± 2.02, 10.53 ± 2.23, and 10.60 ± 1.50 h. The results show that when the concentrations of EZL in chicken cecal contents reached 4.28 ± 1.56 mg/kg, there is a significant therapeutic effect on chicken coccidiosis.
研究领域:
兽医学
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药理学
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寄生虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
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2026
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IF 9.2
Xingdong Yang,Lihua Wu,Chenchen Wang,Yinuo Zhu,Yutong Cao,Keshi Ma,Lili Li,Xiaofei Hu
Ethanamizuril (EZL), a newly developed antiparasitic drug, is expected to be widely used in poultry production in the future, but its residues in eggs and feeds pose potential risks to food safety. This study developed an indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) for the quantitative detection of EZL residues in eggs and feeds. By optimizing the reaction conditions, the ic-CLEIA achieved a sensitivity (half-maximal inhibitory concentration, IC 50 ) of 0.12 ng/mL and a linear range (IC 20 –IC 80 ) of 0.02–0.92 ng/mL. Both the intra-assay and inter-assay coefficients of variation were less than 12%, demonstrating excellent reproducibility and high agreement with HPLC–MS/MS results. The developed ic-CLEIA filling the gap in rapid trace detection of EZL residues, provides a reliable technical support for food safety supervision of EZL residues, and expands the application of CLEIA in veterinary drug residue detection.
✓ 已验证
DOI
PubMed
高影响因子
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