Shi-Xing Liu,Qian Wang,Li Jiang,Shang-Gao Liao,Qing-De Long,Xu Zhang,Xue-Long Yan
Phytochemistry investigation of Euphorbia helioscopia led to the isolation of 22 diterpenoids, including five lathyranes, 11 jatrophanes, and six other types. Among them, eupholine A and B ( 1 and 2 ) are a pair of previously undescribed C-6′ lathyrane epimers. The chemical structures of 1–22 were elucidated using spectral data analysis, and the absolute configurations of 1 together with another jatrophane diterpenoid ( 3 ) were determined by X-ray single-crystal diffraction techniques. Besides, the full nuclear magnetic resonance (NMR) assignments of 3 and 4 were first determined by analysis of their two-dimensional NMR data. All the isolates were screened for inhibition activity of fibrinogen on transforming growth factor-β1-stimulated LX-2 cells, and seven compounds (mainly lathyranes and jatrophanes types) showed certain inhibitory effects at 20 µM, being comparable to that of the positive control, silymarin. Notably, compound 14 (jatrophane type) showed significant inhibitory activity that is superior to silymarin. The study suggested that jatrophane diterpenoids and their analogs (lathyrane types) may serve as a potential chemical entity against liver fibrosis. Graphical
Lili Yu,Zhonghua Li,Huawei Xin,Na Shen,Tao Yan,Yulei Cui
Euphorbia helioscopia L. is a traditional medicinal plant, whose seeds (EHS) are often discarded as agricultural waste. This study aimed to explore the metabolic profile and anti-epithelial-mesenchymal transition (EMT) potential of EHS compared with the conventional whole herb (EHQ). Using UPLC-ESI-MS/MS-based widely targeted metabolomics, 2178 metabolites were identified, with 1423 showing differential accumulation between EHQ and EHS. EHQ was rich in flavonoids, while EHS relatively enriched in lipids compared to EHQ metabolome, enriched in fatty acids such as octanoic, stearic, and octadecenoic acids. Pathway analysis highlighted disparities in lipid and flavonoid biosynthesis. In vitro assays on A549 lung cancer cells revealed that EHS extract possessed stronger cytotoxicity (IC 50 = 0.80 mg/mL) and more potently inhibited cell migration and EMT progression, evidenced by upregulated E-cadherin and downregulated N-cadherin and α-SMA. These findings position EHS as a promising candidate contributors’ lipid-rich resource for anti-EMT drug development, advocating for the valorization of underutilized plant parts in sustainable bioproduct pipelines.
危险品化学品经营许可证（带存储）