Food Bioscience
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2024
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IF 4.8
Dangfeng Wang,Benyou Zhang,Yunting Wang,Likun Ren,Yuqiong Meng,Rui Ma,Shulin Wang,Zhiteng Liu,Yang Jiang,Fangchao Cui,Tingting Li,Jianrong Li
LuxR solos sense exogenous signaling molecules and regulate microbial behaviors, thus facilitating intra- and inter-species communication among bacteria. However, studies on the role of LuxR solos in regulating the spoilage potential of specific spoilage organisms are limited. In this study, we screened a LuxR solo EvgA, derived from Pseudomonas fluorescens PF08. qRT-PCR and molecular docking results indicated that EvgA responds and binds most strongly to exogenous N -3-tetradecanoyl-L-homoserine lactone (C14-HSL) through hydrogen bonds and hydrophobic interactions, with a –CDOCKER ENERGY of 45.87 kcal/mol. Subsequently, the evgA knockout (Δ evgA ) and complementary evgA (Δ evgA -com) mutants of P. fluorescens PF08 were constructed through homologous recombination. Δ evgA mutants displayed diminished eavesdropping to exogenous C14-HSL (by 18.99 ± 2.09%), reduced adhesion ability (by 30.33 ± 0.36%), attenuated biofilm formation (by 15.97 ± 0.14%), and decreased motility (swarming zones by 4.34 ± 0.002 mm and swimming zones by 4.17 ± 0.11 mm). In addition, EvgA deletion reduced spoilage factor production (hydrolysis zone), which included extracellular proteases, lipases, and biogenic amines (by 4.45 ± 0.51 mm, 10.12 ± 0.10 mm, and 14.41 ± 0.57 mm, respectively), thereby diminishing the spoilage of salmon fillets stored at 4 °C. Notably, the addition of exogenous C14-HSL promoted the spoilage ability of all strains. This study emphasizes the important role of LuxR solo EvgA in AHLs-mediated quorum sensing and the spoilage potential of P. fluorescens PF08, which is expected to be a new target for controlling the spoilage of aquatic products.
研究领域:
微生物学
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食品科学
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分子生物学
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生物化学
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DOI
高影响因子
Cell Reports
·
2025
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IF 6.9
Mingfang Wang,Jia Zeng,Binbin Cui,Guangliang Wu,Xiayu Chen,Yikai Fu,Bing Gu,Yinyue Deng
It was previously demonstrated that intestinal pathogens have evolved different mechanisms to enhance their infection and colonization in the intestines of hosts. However, it is unclear how Shigella effectively survives in the presence of bile salts. Here, we report that the biofilm formation and pathogenicity of S . sonnei are induced by primary bile salts through the two-component system EvgS/EvgA. The response regulator EvgA controls the transcription of the cyclic AMP synthase-encoding gene. Furthermore, the effector protein CRP of the cyclic AMP (cAMP) signal not only positively controls the transcription of ydeH , a gene encoding cyclic di-GMP (c-di-GMP) synthase, but also forms a complex with YdeH to improve its catalytic production of c-di-GMP. Additionally, interacting with YdeH enhances the ability of CRP to bind to the promoter of ydeH . Our work provides insights into how S . sonnei utilizes cascade amplification of c-di-GMP to promote fitness and pathogenicity in response to primary bile salts.
研究领域:
微生物学
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分子生物学
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感染与免疫
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信号转导
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病原生物学
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环境应答
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2025
·
IF 4.7
Jinnan Xuan,Shuxian Hou,Yuqiang Han,Chen Li,Yisi Liu,Zhong Li,Xixia Liu,Guoqiang Yang,Xinxin Liu,Jiantao Wang,Yuting Huang,Jun Wang,Wei Lai
Multidrug-resistant bacterial infections have emerged as a global public health crisis due to antibiotic misuse. In this study, we develop a layer-restacked 3D Ti3C2 nanostructure utilizing ice-templating. This nanostructure exhibits outstanding hydrophilicity, biocompatibility, and stability, as well as enhanced absorption, extinction coefficient, and photothermal conversion efficiency. Additionally, the layer-restacked 3D Ti3C2 nanostructure demonstrates excellent antibacterial activity against MDR Escherichia coli and MDR Staphylococcus aureus irradiated by 808 nm near-infrared light (NIR). Specifically, the mechanism of photothermal action against multidrug-resistant bacteria involves structural damage to the bacterial membranes, leading to the leakage of bacterial contents after layer-restacked 3D Ti3C2 nanostructures adhered under NIR irradiation. The results of transcriptome analysis show that the 3D Ti3C2 nanostructure regulates the membrane transporters and membrane transporter proteins on the bacterial cell membrane as well as the activities of enzymes associated with them, which in turn affect the metabolic processes of organic acids and other organic substances in the bacterial cell. The DNA-binding transcriptional activator EvgA is significantly downregulated, which may play a crucial role in inhibiting the emergence of drug resistance in bacteria when exposed to the layer-restacked 3D Ti3C2 nanostructure. The layer-restacked 3D Ti3C2 nanostructure is an effective photothermal antimicrobial nanostructure against multidrug-resistant bacteria.
研究领域:
材料科学
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微生物学
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医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）