The loss of grains in the production chain caused huge waste worldwide, and the control of plant pathogenic fungi during harvest time is a critical solution to mitigate the damage. Eugenol, an important component of clove oil ( Syzygium aromaticum L.), has antioxidant, antifungal, and other activities. In this study, the unstable eugenol was incorporated into the nanoscale γ-cyclodextrin metal-organic framework (γ-CD-MOF), a cubic porous structure synthesized by vapor diffusion method. The investigation of morphologies, functional groups, crystalline structures and weight losing behaviors demonstrated the inclusion complex prepared from γ-CD-MOF and eugenol (CMFE). Compared with the γ-CD loaded with eugenol (CE), CMFE exhibited an improved loading rate with 4.06 % ( w/w ). And CMFE enhanced the stability of its inner eugenol against degradation by light and air. Moreover, the CMFE demonstrated a significant anti -F. graminearum activity both in the medium and on the wheat grains. The inhibition rate reached 83.8 % at 8 mg/mL in vitro , while the growth of the hyphae was effectively restrained at 4 mg/g in vivo . CMFE could damage the mycelial cell wall and trigger intracellular reactive oxygen species (ROS) accumulation, thus exhibiting its anti- F. graminearum activity. Our results showed that the biocompatible CMFE would be an environment-friendly substitute to chemical fungicides for the prevention of fungal contamination caused by F. graminearum .
Yan Zhu,Yuanyuan Yao,Jinfeng Xi,Caiguo Tang,Lifang Wu
Fusarium graminearum ( F. graminearum ) is a vital pathogen of Fusarium head blight (FHB). Hydrogen sulfide (H 2 S), which can be induced by stress in plants as a fungal inhibitor, affects the germination of spores through the detection of environmental factors such as temperature and pH. Based on mathematical models, F. graminearum was treated with different temperatures, pH, and H 2 S concentration to investigate spore germination events. This paper considered the synergistic effects of temperature and pH on spores in order to determine the optimal germination environment. Under these circumstances, the inhibition of spore germination with different concentrations of H 2 S was quantified. The results suggested that the germination time negatively correlates with the germinating rate. An increase in temperature altered the weak alkali preference of F. graminearum spores. At 28 °C, spores were more sensitive to the changes in pH. H 2 S gas could delay the germinating process significantly, however the required H 2 S concentration varied with environmental changes. This paper provides a perspective on the synergistic effect of temperature and pH on spore germination and contributes to determining the concentration of H 2 S as a management of FHB.
危险品化学品经营许可证（带存储）