Jia He,Wei Zhang,Yuyu Cui,Liang Cheng,Xu-Lin Chen,Xianwen Wang
Free radicals are secreted following skin damage and cause oxidative stress and inflammatory reactions that increase the difficulty of wound healing. In this study, copper-based nanozyme Cu 2 Se nanosheets (NSs) are synthesized by an anion-exchange strategy and apply to wounds with F127 hydrogels to investigate the healing effect of this nanozyme composite hydrogels on wounds. Cu 2 Se NSs have a large number of catalytically active centers, are simple to synthesize, require few reaction conditions and have a short synthesis cycle. In vitro experiments have shown that Cu 2 Se NSs possess superoxide dismutase (SOD)-like activity and nitrogen radical scavenging activity and promote angiogenesis and fibroblast migration. The doping of Cu 2 Se NSs into the F127 hydrogel does not have a significantly affect on the properties of the hydrogel. This hybridized hydrogel not only adapts to the irregular and complex morphology of acute wounds but also prolongs the duration of nanozyme action on the wound, thus promoting wound healing. Transcriptomic analysis further reveals the potential therapeutic mechanism of the Cu 2 Se/F127 hydrogel in promoting acute wound healing. Animal experiments have shown that the Cu 2 Se/F127 hydrogel has good biosafety. The Cu 2 Se/F127 hydrogel provides an innovative idea for the development of hydrogel dressings for the treatment of acute wounds.
Xu Jin,Wei Zhang,Jie Shan,Jia He,Haisheng Qian,Xulin Chen,Xianwen Wang
Various injury defense and repair functions are performed by the skin. Free radicals secreted after injury cause oxidative stress and inflammatory responses, which make wound healing difficult. However, the current metal nanomaterials for wound repair do not have sufficient catalytic activity or complex material design and cannot properly fit wounds. Therefore, it is imperative to develop more effective therapeutic approaches. This study investigated the effect of Ni4Cu2 hollow nanospheres composited with F127 hydrogel on promoting wound healing by applying them to wounds. Ni4Cu2 hollow nanospheres exhibited a superior spatial structure, contained many catalytic sites, and could be synthesized in a simple manner. In vitro experiments showed that Ni4Cu2 hollow nanospheres had superoxide dismutase-like activity and promoted fibroblast migration, angiogenesis, and macrophage polarization. F127, which is a thermosensitive, nontoxic, phase-change and porous hydrogel material, has proven to be an effective choice for injectable and sprayable medical dressings. Ni4Cu2 hollow nanospheres were mixed with F127 hydrogel without significantly affecting its performance. In addition to adapting to the complex, irregular gaps of acute wounds, the mixture lengthened the nanozyme release time, which enhanced healing. Based on the animal experiments, the Ni4Cu2/F127 composite hydrogel effectively promoted wound healing, epithelial regeneration, and the formation of skin appendages such as hair follicles in mice. Furthermore, the Ni4Cu2/F127 composite hydrogel was nontoxic to animals and had high biological safety. The Ni4Cu2/F127 composite hydrogel has provided an innovative strategy to develop composite hydrogels for the treatment of acute skin wounds.
Preserving the vital pulp poses significant challenges in clinical pulp injury repair. Tricalcium silicate (C3S)-based cement has emerged as a successful pulp capping biomaterial. However, its efficacy in inducing pulp-dentin complex regeneration requires enhancement, and handling difficulties, poor anti-collapsible ability and inadequate self-setting performance remain significant obstacles, restricting its broader application in endodontics. In this study, a multifunctional SrCO 3 -doped C3S biocement (C3S-Sr/F127-nHA) reinforced by Pluronic® F127 thermoreversible hydrogel and nano hydroxyapatite (nHA) was developed to overcome the limitations of C3S-based cements. The thermoresponsive characteristic of F127 conferred the C3S pastes smart response behavior, allowing for optimal operability at room temperature and maintaining excellent anti-collapse properties at body temperature. C3S-Sr/F127-nHA exhibited an injectability rate of up to 93 %. nHA facilitated the hydration kinetics of C3S, resulting in reduced setting time and enhanced compressive strength. Furthermore, C3S-Sr/F127-nHA demonstrated excellent apatite mineralization ability, cytocompatibility, and potential for osteo/odontogenic induction. The inclusion of Sr 2+ ions and nHA promoted osteo/odontogenic differentiation of stem cells isolated from human exfoliated deciduous teeth (SHED). In vivo findings indicated that C3S-Sr/F127-nHA could stimulate the formation of a homogeneous dentin bridge. Consequently, C3S-Sr/F127-nHA is a promising biocement for use in regenerative endodontics.
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