Da-Peng GUO,Yong-Huan WANG,Tian-Bai XU,Yuan ZHANG,Ling-Feng Li,Jin-Kai CHEN,Xiao-Zhi WANG,Peng LI,Ji-Kui LUO
The microchip field asymmetric ion mobility spectrometry (FAIMS) technology was used for rapid analysis of phenylalanine (PHE) using the metal diffusion tube-microchip μ-FAIMS system. The test pressure was set at 0.25 MPa, and the temperature of the gas generator for the diffusion tube was at 190 °C. The ion characteristic spectra were acquired under the optimized conditions with a gas flow rate of 2000 mL min −1 , a dispersion voltage of 152.8 V and a compensation voltage of −0.62 V for phenylalanine in positive mode. Phenylalanine gas samples with different concentrations were analyzed in detail by FAIMS. The results showed that the ion intensity was linearly correlated with phenylalanine concentration in the range from 6 mg L −1 to 20 mg L −1 , with the detection limit of 5.93 mg L −1 for phenylalanine. This investigation shows the feasibility of FAIMS technique for rapid detection of phenylalanine. The FAIMS instrument is simple, and has high sensitivity and fast response, and shows great potential for rapid detection of phenylalanine.
Qi Wang,Yun-Fei Xie,Wei-Jun Zhao,Peng Li,He Qian,Xiao-Zhi Wang
A fast and quantitative method for the detection of biogenic amines based on the microchip-based high-field asymmetric waveform ion mobility spectrometry (FAIMS) technique was established for evaluating the degree of spoilage of pork stored at room temperature. Trimethylamine (TMA) was selected as a good indicator for its volatility and high proton affinity. Analysis of standard TMA showed the same characteristic peak with the pork sample. The limit of detection of the system for TMA dissolved in deionized water, with 100 μL of 8 mol L−1 NaOH, was 3 ng and the measurement was accomplished within 15 s. The relative standard deviation (RSD) was 6.20%. The delivery of the sample gas was based on the dynamic headspace method. The effect of the storage time on the formation of biogenic amines was examined, and not surprisingly, the content of TMA increases over time. The total volatile basic nitrogen (TVB-N) was measured simultaneously based on the classical semi-micro Kjeldahl method. A good correlation coefficient between these two methods was obtained, the correlation coefficient was 0.952. Therefore the microchip-based FAIMS technique provides a quantitative classification of the spoilage or freshness of pork, especially when a rapid detection is needed.
危险品化学品经营许可证（带存储）