JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
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2025
·
IF 3.1
Zhengrui Hu,Han Wang,Shan Li,Youjiang Liu,Chunjing Xu,Shaomin Liu,Jianhui Wei,Chilai Chen
By introducing the strengths of the vertical interconnection of TGV (Through Glass Via) technology into FAIMS (high-Field Asymmetric waveform Ion Mobility Spectrometry) chip manufacturing, we propose a wafer-level TGV-MS (multi-stack) FAIMS chip manufacturing method and achieve the wafer-level fabrication of 14 chips on a 6-inch glass wafer for the first time. The test results demonstrate that the TGV wafer manufactured based on this method shows excellent airtightness, electrical properties, and consistency with a leakage rate of $3.94\times 10 ^{-11}$ Pa $\cdot $ m3/s, a resistivity of $1.92\times 10 ^{-4}~\Omega \cdot $ cm. A single FAIMS chip volume is $4.8\times 1.6 \times 0.16$ cm3, weight is 2.28 g, the relative thickness error is less than 0.3%, and the parallelism error is less than 0.01°. Meanwhile, typical volatile organic compounds benzene and toluene were selected as samples to test the separation performance of the FAIMS chip, and the detection spectra under different gas concentrations and voltages were obtained. The experimental results show that the wafer-level FAIMS chip has a good resolution for benzene and toluene, and the linear detection range for the two samples is 0.1 ppm to 2 ppm (linear fitting coefficients 98.9%, 99.5%). The detection limit for benzene and toluene is 77 ppb. This paper solves the problem that microfluidic structures with electrodes cannot be manufactured at the wafer level due to side planar electrode leads, greatly simplifies the structure of the chip, realizes the mass production of FAIMS chips, and provides a technical reference for the wafer-level manufacturing of analysis sensors such as microfluidic chips with electrode structures and MEMS mass analyzers. [2024-0229]
研究领域:
微制造技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 9
·2965-2972
·
IF 1.938
Qi Wang,Yun-Fei Xie,Wei-Jun Zhao,Peng Li,He Qian,Xiao-Zhi Wang
A fast and quantitative method for the detection of biogenic amines based on the microchip-based high-field asymmetric waveform ion mobility spectrometry (FAIMS) technique was established for evaluating the degree of spoilage of pork stored at room temperature. Trimethylamine (TMA) was selected as a good indicator for its volatility and high proton affinity. Analysis of standard TMA showed the same characteristic peak with the pork sample. The limit of detection of the system for TMA dissolved in deionized water, with 100 μL of 8 mol L−1 NaOH, was 3 ng and the measurement was accomplished within 15 s. The relative standard deviation (RSD) was 6.20%. The delivery of the sample gas was based on the dynamic headspace method. The effect of the storage time on the formation of biogenic amines was examined, and not surprisingly, the content of TMA increases over time. The total volatile basic nitrogen (TVB-N) was measured simultaneously based on the classical semi-micro Kjeldahl method. A good correlation coefficient between these two methods was obtained, the correlation coefficient was 0.952. Therefore the microchip-based FAIMS technique provides a quantitative classification of the spoilage or freshness of pork, especially when a rapid detection is needed.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 3
·1036-1045
·
IF 1.938
Xiaozhi Wang,Weijun Zhao,Lingfeng Li,Xiangyang Wang,Peng Li,Yu Wang,Jikui Luo
Sulfites (sulfur dioxide) and inorganic sulfites are types of food additives and preservatives, widely used in the production of food and herbal medicines (HMs). However, the overingestion of sulfites and its associates are harmful to human health and may cause medical complications. Various methods and instruments have been developed for measuring sulfites in foods and HMs, but they typically suffer from many issues, such as high detection limitation, inaccurate and non-reliable results, time and labor-intensive sample preparation, and high cost. Herein, we present a fast, sensitive, and quantitative method to determine sulfites in HMs using field asymmetric-wave ion mobility spectrometry (FAIMS) coupled with a headspace bubbling method. The headspace air bubbling method is effective and efficient at generating stable sulfur dioxide (SO2) in the gas phase for FAIMS analysis. It allows sulfites with a concentration down to 1 mg kg−1 to be easily detected by this new method in 20 min, much shorter than with current technologies. The limits of detection (LOD) and limits of quantification (LOQ) are 1 mg kg−1 and 3 mg kg−1 in HMs, respectively. The new method is of great significance to ensure medical safety and for HM production quality control.
研究领域:
分析化学
·
药学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2016
·
IF 0.566
Da-Peng GUO,Yong-Huan WANG,Tian-Bai XU,Yuan ZHANG,Ling-Feng Li,Jin-Kai CHEN,Xiao-Zhi WANG,Peng LI,Ji-Kui LUO
The microchip field asymmetric ion mobility spectrometry (FAIMS) technology was used for rapid analysis of phenylalanine (PHE) using the metal diffusion tube-microchip μ-FAIMS system. The test pressure was set at 0.25 MPa, and the temperature of the gas generator for the diffusion tube was at 190 °C. The ion characteristic spectra were acquired under the optimized conditions with a gas flow rate of 2000 mL min −1 , a dispersion voltage of 152.8 V and a compensation voltage of −0.62 V for phenylalanine in positive mode. Phenylalanine gas samples with different concentrations were analyzed in detail by FAIMS. The results showed that the ion intensity was linearly correlated with phenylalanine concentration in the range from 6 mg L −1 to 20 mg L −1 , with the detection limit of 5.93 mg L −1 for phenylalanine. This investigation shows the feasibility of FAIMS technique for rapid detection of phenylalanine. The FAIMS instrument is simple, and has high sensitivity and fast response, and shows great potential for rapid detection of phenylalanine.
研究领域:
分析化学
·
医学检验
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Qingchi Wang,Yuhang Luo,Yi Xin,Yuanxiang Jin,Guiling Yang,Quanbin Zhang,Zhengshun Wen
Marine polysaccharides have emerged as promising immunomodulators. However, conventional analytical approaches have failed to elucidate their activation mechanisms from a global perspective. In this study, we isolated a highly branched glycogen (GCEP2a) from eggs of the sea urchin Glyptocidaris crenularis . Structural characterization revealed that GCEP2a possesses a backbone composed of →4)-α-D-Glc p -(1 → residues, with one C6-branch per five residues, and a molecular weight of 8.06 × 10 3 kDa. Employing nascent-proteome bioorthogonal non-canonical amino acid tagging (BONCAT) enrichment followed by DIA-FAIMS, we unraveled the global immunostimulatory mechanisms of GCEP2a in RAW264.7 macrophages. This advanced approach enabled dynamic profiling of nascent protein synthesis, uncovering a multi-receptor and multi-pathway regulatory network. Specifically, GCEP2a activates signaling consistent with engagement of the toll-like receptors (TLRs), C-type lectin receptors (CLRs), and RIG-I-like receptors (RLRs), thereby triggering downstream signaling cascades including the NF-κB, MAPK, and PI3K-AKT pathways. Additionally, GCEP2a downregulates the biosynthesis of lacto- and neolacto-series glycosphingolipids, indicating metabolic reprogramming associated with M1 polarization. These findings not only suggest that GCEP2a exhibits potential as an immune adjuvant, but also provide initial insights into the application of BONCAT-based nascent proteomics for investigating the multi-modal immunostimulatory mechanisms of marine polysaccharides.
研究领域:
免疫学
·
海洋生物化学
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糖生物学
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蛋白质组学
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分子免疫学
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细胞信号转导
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）