Bioanalysis
·
2023
·
IF 1.8
Yuhuan Ji,Zhaoxu Guo,Min Yan,Limin Chu,Min Meng,Yantao Chu,Hong Yu,Laixin Wang
Aim: RBD1016 is an N-acetylgalactosamine-conjugated siRNA drug currently in a phase II trial for treatment of chronic hepatitis B virus. To evaluate its absorption, distribution, metabolism and excretion (ADME) and pharmacokinetic/pharmacodynamic (PK/PD) properties, two LC-based bioanalytical methods, LC–high-resolution/accuracy MS and LC–fluorescence detection, were developed and qualified. Materials & methods: The LC–high-resolution/accuracy MS method was used for metabolite identification and simultaneous quantitation of the antisense and sense strands as well as their respective metabolites. The LC–fluorescence detection assay was primarily used for analyzing the antisense strand and its metabolites in low-concentration plasma samples. The two methods were successfully bridged by analyzing the same sets of study samples. Results & conclusion: Both methods were found to have excellent accuracy/precision, specificity and reproducibility to support ADME and PK/PD studies of RBD1016 siRNA.
研究领域:
药学
·
分析化学
·
药理学
·
病毒学
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2012
·
IF 4.98
Xiaoyan Wang,Zuoming Zhou,Guohua Jing
Magnetic poly(styrene–glycidyl methacrylate) porous microspheres (MPPM) with high magnetic contents were prepared by surfactant reverse micelles and emulsion polymerization of monomers, in which the well-dispersed Fe 3 O 4 nanoparticles were modified by polyethylene glycol (PEG) and oleic acid (OA) respectively. The characterizations showed that both of the OA–MPPM and the PEG–MPPM were ferromagnetic, however, the OA–MPPM was used to immobilize the bacteria for more advantages. Therefore, the effects of monomer ratio, surfactant, crosslinker and amount of Fe 3 O 4 on the structure, morphology and magnetic contents of the OA–MPPM were investigated. Then, the OA–MPPM was utilized to immobilize Klebsiella sp. FD-3, an iron-reducing bacterium for Fe(III)EDTA reduction applied in NO x removal. Compared with free bacteria, the immobilized FD-3 showed a better tolerance to the unbeneficial pH and temperature conditions.
研究领域:
环境科学
·
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2014
·
IF 5.039
Zuo-Ming Zhou,Xiao-Yan Wang,Tian-Ming Lin,Guo-hua Jing
Fe 3 O 4 poly (styrene-glycidyl methacrylate) magnetic porous microspheres (MPPMs) were introduced to immobilize Klebsiella sp. FD-3, an iron-reducing bacterium applied to reduce Fe(III)EDTA. The effects of potential inhibitors (S 2− , SO 3 2− , NO 3 − , NO 2 − and Fe(II)EDTA-NO) on Fe(III)EDTA reduction were investigated. S 2− reacted with Fe(III)EDTA as an electron-shuttling compound and enhanced the reduction. But Fe(III)EDTA reduction was inhibited by SO 3 2− and Fe(II)EDTA-NO due to their toxic to microorganisms. Low concentrations of NO 3 − and NO 2 − accelerated Fe(III)EDTA reduction, but high concentrations inhibited the reduction, whether by free or immobilized FD-3. The immobilized FD-3 performed better than freely-suspended style. The substrate mass transfer and diffusion kinetics in the porous microspheres were calculated. The value of Thiele modulus and effectiveness factors showed that the intraparticle diffusion was fairly small and neglected in this carrier. Fe(III)EDTA reduction fitted first-order model at low Fe(III)EDTA concentration, and changed to zero-order model at high concentrations.
研究领域:
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Lin Zhu,Xinlei Wang,Xuebo Song,Fuping Zheng,Hehe Li,Feng Chen,Yuhang Zhang,Fuyan Zhang
Changes of key odorants and aroma profiles of Chinese Laowuzeng baijiu during its one-year ageing were determined by HS-SPME–AEDA and direct injection–AEDA (DI–AEDA). Ethyl hexanoate, ( E,E )-2,4-decadienal, and 2-phenylethyl acetate showed the highest FD value (486) in all ageing stages. With regards to aroma profiles, fruity, floral, acidic, sweet/honey and cheesy aromas were enhanced during storage, while pickled vegetable, grain and alcoholic notes weakened during the ageing. Quantitation and OAVs showed that most of the aroma compounds (OAVs > 1), including ethyl esters, aldehydes, and acids, increased their contents within the same period, whereas nonanal, 2-phenylethyl acetate, ethyl benzoate, 4-ethylguaiacol, propanol and 3-methyl-1-butanol decreased in content after the storage of 365 days. Simulated aged samples, in which fresh samples were spiked with 18 compounds, were examined by triangle tests, which indicated that the “fruity” compounds were crucial for maintaining the special aroma profile of an aged sample.
研究领域:
食品科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IRANIAN POLYMER JOURNAL
·
2023
·Vol. 32
·期 10
·1229-1240
·
IF 3.1
Song Lixin,Zhang Jian,Zhang Xing,Rui Chaofan,Zhang Yunxia,Wang Mingyu,He Juan
The surface imprinting polymers (HKUST-1 @ MIPs) were synthesized by molecular imprinting technology. Using the metal–organic framework (HKUST-1) as the carrier, 4-methylumbelliferyl acetate as alternative templates for aflatoxins, and molecularly imprinted polymers polymerized on the surface of HKUST-1. The adsorption capacity (6.0 μg·mg −1 ) and adsorption rate (adsorption equilibrium reached within 5 min) of HKUST-1 @ MIPs for aflatoxins were studied. HKUST-1 @ MIPs were used as adsorbents to prepare surface-imprinted solid-phase extraction columns (HKUST-1 @ MIPs SPE columns). The extraction conditions of HKUST-1 @ MIPs SPE columns were optimized, and the reliability of the method was verified by HPLC–FD technique. Water was chosen as the wash solution and methanol as the eluent with an elution volume of 2 mL. The linear range of the method was 0.5–20 μg·kg −1 with R 2 = 0.9991–0.9996. The detection limits of aflatoxins (G2, G1, B2, and B1) were 0.05, 0.02, 0.03, and 0.05 μg·kg −1 , respectively. The limits of quantification were 0.2, 0.1, 0.15, and 0.3 μg·kg −1 , accordingly. The spiked recovery of aflatoxins (G2, G1, B2, and B1) was 92.5%-118.4%, and the relative standard deviation was 3.14%-6.03%. It had been verified that the self-made solid-phase extraction column had high stability and reusability and could be used for at least six times. Comparing the HKUST-1 @ MIPs SPE and immunoprophylactic columns, a low-cost, easy-to-operate, and sensitive method was developed for detecting trace aflatoxins in food by combining with HPLC–FD technique. Graphical abstract
In this work, dissolve BTC and Cu (NO3)2·3H2O completely and stir magnetically, heat to 120 °C for 12 hours. After washing with solvent, the purple solid HKUST-1 was obtained by centrifugation and drying
研究领域:
食品安全
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2023
·Vol. 38
·期 11
·2595-2607
·
IF 4.5
Jue Wang,Sai Gu,Bo Qin
Background Exosomes are applied as biomarkers in several diseases according to their disease-specific profiles. However, the exosomes effects in functional dyspepsia (FD) are still fragmentary. Here we examined the role of Eosinophil and mast cell derived-exosomes in FD progression. Methods Fifty FD subjects and age- and sex-matched healthy controls were included in this retrospective cohort study. Duodenal mucosa and gastric juice were collected to analyze molecular difference. Eosinophil and mast cell were evaluated by immunofluorescence and microarray was subjected to examine the expression levels of NEAT1, miR-211-5p, and glial cell line-derived neurotrophic factor (GDNF), which were subsequently were tested by quantitative reverse transcription PCR (RT-qPCR) validation cohorts. CCK-8 assays, and wound healing assays were used to evaluate integrity of intestinal mucosal barrier in vitro. Rats' weights and gastric emptying rates were used as evaluation of FD severity in vivo. Results Eosinophil and mast cell were enriched and secreted more exosomes in duodenal mucosa of FD patients. We identified differential lncRNAs that were consistently and significantly up regulated in FD cases. Of these, NEAT1 was further validated by RT-qPCR and had closely relationship with GDNF. MiR-211-5p level was found to be reduced in FD and negatively related with NEAT1 and GDNF. Furthermore, NEAT1and GDNF relived FD while miR-211-5p made symptoms worse. The NEAT1/miR-211-5p/GDNF axis had a good predictive ability for FD. Conclusions The NEAT1/miR-211-5p/GDNF could be a potential FD biomarker.
研究领域:
胃肠病学
·
分子生物学
·
病理生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 7
·1492
·
IF 4.35
Jia Huang,Haitao Chen,Zhimin Zhang,Yuping Liu,Binshan Liu,Baoguo Sun
To investigate the key odor-active compounds in children’s soy sauce (CSS), volatile components were extracted by means of solvent extraction coupled with solvent-assisted flavor evaporation (SE-SAFE) and solid-phase microextraction (SPME). Using gas chromatography-olfactometry (GC-O) and gas chromatography-mass spectrometry (GC-MS), we identified a total of 55 odor-active compounds in six CSSs by comparing the odor characteristics, MS data, and retention indices with those of authentic compounds. Applying aroma extract dilution analysis (AEDA), we measured flavor dilution (FD) factors in SE-SAFE isolates, ranging from 1 to 4096, and in SPME isolates, ranging from 1 to 800. Twenty-eight odorants with higher FD factors and GC-MS responses were quantitated using the internal standard curve method. According to their quantitated results and thresholds in water, their odor activity values (OAVs) were calculated. On the basis of the OAV results, 27 odorants with OAVs ≥ 1 were determined as key odorants in six CSSs. These had previously been reported as key odorants in general soy sauce (GSS), so it was concluded that the key odorants in CSS are the same as those in GSS.Keywords:children soy sauce;gas chromatography-olfactometry;AEDA;FD factor;quantitative measurements;OAV;key odorants
研究领域:
食品科学
·
分子感官科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2014
·
IF 4.384
Shaoxin Deng,Chengxiang Shi,Xueyan Xu,Hui Zhao,Pingchuan Sun,Tiehong Chen
A novel bioinspired approach for ordered mesoporous silica was developed on the basis of the synergic coassembly between polyamine and an anionic surfactant as a template. With the help of cationic polyamine, anionic surfactant micelles could be utilized as a mesostructure template, whereas with the aid of the anionic surfactant micelles the cationic polyamine chains underwent aggregation to exert their ability to induce silica condensation. Mesoporous silicas with well-ordered mesostructure of Fd-3m symmetry and 3D hexagonal close-packed mesostructure (hcp) were fabricated. Because of the abundant types of anionic surfactants and polyamines, the synthesis approach can be regarded as a general method for anionic-surfactant-templated mesoporous silica, and new mesostructures and morphologies are expected.
研究领域:
材料科学
·
生物仿生学
·
胶体与界面化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2025
·
IF 4.2
Xia Yang,Yun Wang,Yan Sun,Jiali Guo,Zhaojun Geng,Bingyao Wei,Peiqin Li,Guanghui Tang
Leaf rust caused by Coleosporium zanthoxyli is a destructive disease that threatens Zanthoxylum bungeanum plantations. To address this, screening for resistant cultivars and exploring their resistance mechanisms are crucial for effective disease management. However, the mechanisms underlying Z. bungeanum 's resistance remain unclear. In this study, we conducted in vivo and in vitro experiments to screen for the resistant cultivar Qiujiao (QJ) and the susceptible cultivar Fengxian Dahongpao (FD). Subsequently, the resistance mechanisms of Z. bungeanum were analyzed by integrating transcriptome and metabolome, the result showed the flavonoid pathway served as the primary metabolic pathway for QJ to confer resistance against C. zanthoxyli . Upon infection with C. zanthoxyli , the key genes ( PAL, CHI , and HCT ) and enzymes (PAL, C4H, and 4CL) involved in the flavonoid pathway were upregulated in QJ, and the total flavonoid content was increased in QJ. Additionally, eight flavonoids from this pathway demonstrated inhibitory effects on C. zanthoxyli spore germination, with naringenin and phloretin exhibiting the stronger activity. Collectively, the flavonoid pathway plays a dominant role in the resistance of Z. bungeanum leaves against C. zanthoxyli .
研究领域:
植物病理学
·
分子生物学
·
转录组学
·
代谢组学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Dong-Yu Shen,Huan-Lu Song,Ting-Ting Zou,Su-Yan Wan,Meng-Ke Li
The leaf of moso bamboo ( Phyllostachys pubescens Mazel ) is rich in odorant compounds, which is important natural materials for the production of flavor. It also contains phenolic acids, amino acids and peptides, which is a potential source of natural bioactive compounds. The study of odor-active compounds in bamboo leaves can provide a basis for the discovery of natural flavor. The leaf, stem, and powder of moso bamboo were analyzed by gas chromatography-ion mobility spectrometry (GC-IMS). Main odor-active compounds in moso bamboo leaf were analyzed and characterized by (1) a gas chromatography olfactory mass spectrometry (GC-O-MS), (2) two-dimensional gas chromatography olfactory mass spectrometry (GC × GC-O-MS) and (3) electronic nose (E-nose). Based on aroma extract dilution analysis (AEDA), 13 key odor-active compounds with high flavor dilution (FD) factor (≥27), including 3-methyl-1-butanol, ( E )-2-hexenal, ethyl hexanoate, ( Z )-4-heptenenal, 6-methyl-5-hepten-2-one, octanal, ethyl ( Z )-3-hexenoate, 1-hexanol, ( Z )-3-hexen-1-ol, ( E, E )-2,4-heptadienal, ( Z )-2-hexen-1-ol, 1-octen-3-ol and benzaldehyde, were further analyzed. The compounds detected by the above four methods were ( E )-2-hexenal, 6-methyl-5-hepten-2-one, octanal, ( E, E )-2,4-heptadienal, 1-octen-3-ol and benzaldehyde, and all of which were the main and potential odorants of moso bamboo leaf.
研究领域:
植物学
·
分析化学
·
食品科学
·
风味化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Sam Al-Dalali,Fuping Zheng,Baoguo Sun,Feng Chen
Aroma profiles and aroma-active compounds of Sichuan vinegar, which is one of the four famous vinegars in China, were systemically analyzed by solvent-assisted flavor evaporation-gas chromatography-mass spectrometry (SAFE-GC-MS) and gas chromatography-olfactometry (GC-O). In addition, descriptive profile analysis, aroma reconstitution, and omission test were used to evaluate and compare the Sichuan modern vinegar (SMV) and Sichuan traditional vinegar (STV). A total of 99 volatile compounds were tentatively identified from the neutral and acidic fractions of both samples. Among them, 77 compounds were positively identified after comparison with their corresponding standards. Forty-two aroma-active compounds were characterized with flavor dilution (FD) factors from 1 to 6561 by aroma extract dilution assay (AEDA)-GC-O, with the highest for 2-hydroxy-3-butanone, butyrolactone, furan-2-carbaldehyde, acetic acid, and 3-oxobutan-2-yl acetate in both STV and SMV samples. Among them, 10 were identified for the first time in vinegar. Moreover, 40 aroma-active compounds were quantitatively determined, and 26 compounds exhibited their odor activity values (OAVs) larger than 1. The reconstituted solutions showed similar aroma profiles to the original samples in their characteristic aromas in terms of fruity, sweet, roasty, spicy, and woody notes but had slight differences in nutty and herbal notes.
研究领域:
食品科学
·
化学感官科学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）