CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Gang Shu,Xinyi Cai,Yu Zhang,Haoyu Wang,Jinbin Pan,Shao-Kai Sun,Cai Zhang
Photothermal-immunotherapy is a highly promising approach for tumor combination treatment, but developing photoimmunotherapy agents with the potential for clinical translation continues to present a considerable challenge. Herein, we developed a plug-and play prussian blue-Mn gel (PBM gel) for in vivo tumor photoimmunotherapy. The PBM gel was rapidly synthesized within minutes by simply mixing Fe 3+ , Fe(CN) 6 4- , sodium alginate (ALG) solution, and Mn 2+ , without the need for further purification (100 % yield), making it ready for immediate use. The PBM gel offers several key advantages, including a simple composition, an easy-to-follow synthesis process, cost-effectiveness, and large-scale production ability (over 1000 g in a single batch). Furthermore, PBM gel exhibited outstanding near-infrared II photothermal heating capability (23 % of photothermal conversion efficiency), immune activation effects, and Fenton-like catalytic activity. In a mice tumor model, the PBM gel effectively suppressed tumor growth by combining photothermal and immunotherapy at a lower power density without obvious side effects. Given that all components of the PBM gel are FDA-approved drugs or biomaterials, and considering its plug-and-play nature, the proposed PBM gel offers a novel clinically translatable strategy for the development of powerful tumor combination treatments.
研究领域:
癌症治疗
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材料科学
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生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Small Methods
·
2023
·
IF 12.4
Xinxin Hang,Zhaocun Huang,Shiqi He,Zhiying Wang,Zaizai Dong,Lingqian Chang
In vitro, drug assessment holds tremendous potential to success in novel drug development and precision medicine. Traditional techniques for drug assessment, however, face remarkable challenges to achieve high speed, as limited by incubation-based drug delivery (>several hours) and cell viability measurements (>1 d), which significantly compromise the efficacy in clinical trials. In this work, a nano-electroporation-DNA tensioner platform is reported that shortens the time of drug delivery to less than 3 s, and that of cellular mechanical force analysis to 30 min. The platform adopts a nanochannel structure to localize a safe electric field for cell perforation, while enhancing delivery speed by 10 3 times for intracellular delivery, as compared to molecular diffusion in coculture methods. The platform is further equipped with a DNA tensioner to detect cellular mechanical force for quantifying cell viability after drug treatment. Systematic head-to-head comparison, by analyzing FDA (food and drug administration)-approved drugs (paclitaxel, doxorubicin), demonstrated the platform with high speed, efficiency, and safety, showing a simple yet powerful tool for clinical drug screening and development.
研究领域:
生物技术
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药学
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细胞生物学
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药物开发
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精准医学
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2025
·
IF 4.3
Wenqingqing Kang,Henry H. Y. Tong,Shu Li
Tyrosinase, a copper-dependent oxidase, plays a critical role in melanin biosynthesis and is a target in skin-whitening cosmetics. Conventional inhibitors like arbutin and kojic acid are widely used but suffer from cytotoxicity, instability, and inconsistent efficacy, highlighting the need for safer, more effective alternatives. In this study, two ligand-based machine learning models were developed: one to predict the biological activity of compounds and the other to estimate specific pIC50 values. These models were employed to screen potential tyrosinase inhibitors from natural product libraries and FDA-approved drug databases. Subsequently, the molecules identified through machine learning screening were subjected to more precise multi tyrosinase-like structures molecular docking to refine the selection. We identified three top-ranking inhibitors, rhodanine-3-propionic acid, lodoxamide, and cytidine 5′-(dihydrogen phosphate), with strong binding affinities mediated by metal ion coordination and π–π interactions at the enzyme’s active site. In vitro assays revealed that all three compounds exhibited higher inhibitory activity against mushroom tyrosinase compared to arbutin (IC50 = 38.37 mM), with rhodanine-3-propionic acid displaying the most potent inhibition (IC50 = 0.7349 mM). Furthermore, transdermal permeation experimental results confirmed that these compounds achieved markedly better skin permeability than commercial arbutin-based formulations, highlighting their potential as next-generation agents for inhibiting melanin production in cosmetic applications.
研究领域:
生物信息学
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计算生物学
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药学
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皮肤科学
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化妆品科学
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酶学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 15
·5252-5259
·
IF 3.978
Lujie Yang,Quanqing Zhang,Yuanyu Huang,Ling Lin,Hartmut Schlüter,Ke Wang,Cuiping Zhang,Pengyuan Yang,Hongxiu Yu
Glycosylation is an important mechanism of secondary protein processing. Large-scale profiling of glycopeptides released by proteolytic digestion of glycoproteins from biologic samples with complex compositions is limited due to their low abundance. Herein, we present a multimodal material based on boronic acid-modified mesoporous magnetic particles with a hydrophilic surface and enlarged pores around 10 nm. Multimodal enrichment successfully improved the enrichment specificity and efficiency of BMMP by synergistic interaction of hydrophilicity and boronic acid functional groups. The 10 nm pore size allows glycopeptides to enter the channel. Hydrophilic glycopeptides could be selectively enriched with an extremely low limit of detection (0.33 fmol per μL) and a high selectivity (1 : 100). From 2 μL of human serum, 328 unique glycopeptides from 101 glycoproteins were identified. A total of 33% of those glycoproteins overlapped with FDA-cleared blood serum biomarkers. It is expected that BMMP in the future can be used for large-scale biomedical glycoproteomics studies.
研究领域:
分析化学
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蛋白质组学
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生物化学
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生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）