The recruitment of the phosphorus-solubilizing rhizobacteria plays an important role in response to phosphorus deficiency. Through the treatments of Arabidopsis thaliana (Col-0) and the FERONIA (FER) functional deficient mutants ( fer-4 and fer-5 ) with the soil suspension in various phosphorus conditions, we discovered that FER could promote phosphorus-solubilizing rhizobacteria enrichment to rescue the defective plant during phosphorus deficiency. The amplicon sequencing data reflected that the phosphorus-solubilizing rhizobacterial genus Alcaligenes was significantly enriched of Col-0 than fer-4 in low phosphorus conditions. Metabolomics analysis revealed that there were more α-D-Glucose (α-D-Glc) and L-Leucine (L-Leu) in Col-0 roots than those in fer-4 roots. The alterations of α-D-Glc and L-Leu mediated by FER had high-positive correlations to the enrichment of Alcaligenes . We successfully isolated a phosphorus-solubilizing rhizobacteria strain identified as Alcaligenes faecalis PSB15. The α-D-Glc and L-Leu could promote the strain PSB15 growth on LB agar plates and assist fer-4 in recovering from phosphorus starvation in the low phosphorus (LP) liquid medium vermiculite with tricalcium phosphate (TCP). The α-D-Glc and L-Leu could be considered as promising compounds to enrich beneficial phosphorus-solubilizing rhizobacteria, such as Alcaligenes , and provide a reference for overcoming the plight of phosphorus deficiency in crops in the field of agricultural production in the future.
tRNA fragments (tRFs) are a recently identified class of small noncoding RNAs. To date, the regulation of tRF abundance and its functional mechanisms have been largely unclear in plants. We investigated how the Arabidopsis thaliana receptor kinase FERONIA (FER) regulates the abundance of tRFs to inhibit global mRNA translation. We demonstrate that FER regulates tRF abundance by directly phosphorylating the tRNA-binding protein YUELAO (YL) to modulate its function. Downregulation of FER and YL prevented the modification of tRNA via cytosine-5-methylation and 2'-O-methylation, thereby increasing tRF abundance. Furthermore, we show that YL acts as an important genetic downstream target of FER signaling, and knockdown of a specific tRF partially rescues the root hair growth defects of fer and yl mutants. Our findings shed light on the abundance and regulatory mechanisms of tRF and their role in inhibiting translation in plants.
CrRLK1L receptor kinases, particularly FERONIA ( FER ), play critical roles in plant stress adaptation and hormonal signaling, yet their functional characterization in economically vital crops like Brassica napus remains limited. Here, we identified 108 CrRLK1L family members through genome wide screening in B. napus and focused on BnFERONIA , a homolog evolutionarily conserved with FERONIA orthologs in Arabidopsis and related crucifers. CRISPR/Cas9 generated BnFERONIA knockout mutants ( cas9-fer ) in Arabidopsis exhibited contrasting abiotic stress responses: hypersensitivity to salt stress (100 mM NaCl), with root elongation was significantly inhibited compared to wild-type, and enhanced tolerance to osmotic stress (300 mM mannitol), displaying 20% longer roots. Furthermore, cas9-fer mutants showed pronounced ABA hypersensitivity, with germination rates significantly suppressed under 0.25 μM ABA and vegetative growth stunted, indicating the role of BnFERONIA in attenuating ABA signaling. Strikingly, osmotic-induced germination remained unaffected in mutants, suggesting there was a compensatory mechanisms during the early stress perception. This research provides the first functional evidence of BnFERONIA gene during stress adaptation, offering a molecular framework for creating stress-resistant oilseed crops by specifically manipulating the CrRLK1L pathways.
危险品化学品经营许可证（带存储）