SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xianli Zong,Rong Zhu
This paper reports a high-performance glucose biosensor using ZnO nanorod-based field effect transistor (FET) to continuously monitor glucose concentration. Sensing area reduction of the sensor for minimally-invasive glucose monitoring with high sensitivity and good stability is demonstrated. The biosensor is fabricated via a hydrothermal growth of semiconducting ZnO nanorods between source and drain micro-electrodes incorporating with alternating current (AC) electric-field control. ZnO nanorod structured FET acts as a frequency mixer to transduce glucose concentration into a current change at certain difference frequency, which is measured by a lock-in amplifier. Due to the large surface-to-volume ratio of ZnO nanorods and the advanced frequency mixing detection scheme, good anti-jamming capability and long-term stability are realized. The glucose sensor achieves a high sensitivity of 1.6 mA/(μM-cm 2 ), a glucose concentration detection limit of 1 μM, and excellent long-term stability shown in 38-h continuous monitoring. In contrast to the state-of-the-art glucose sensors, the developed biosensor exhibits competitive advantages in sensitivity, long-term stability, tiny size and fabrication cost, which indicates its promising application potential in wearable continuous glucose monitoring for diabetics.
研究领域:
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
STAR Protocols
·
2023
·
IF 0
Kun Li,Chunzi Liang,Yuling Pan,Linghan Mei,Guo-Jun Zhang
Summary Here, we present a protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection. We describe steps for preparing graphene quantum dot-phosphorodiamidate morpholino oligomer hybrids, depositing them onto the reduced graphene oxide field surface, hybridizing analyte miRNA with the sensor probe, and capturing and calculating electrical signals. We also detail procedures for optimizing biosensor construction and evaluating performance. By quantifying plasma exosomal miRNA21, this protocol can identify cancer patients from healthy individuals. For complete details on the use and execution of this protocol, please refer to Li et al. 1
研究领域:
纳米技术
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生物传感器
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分子生物学
·
癌症研究
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DOI
PubMed
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2024
·
IF 2.9
Kaibo Yuan,Qinqin Qian,Miaomiao Wu,Bingxia Wang,Shuwen Zeng,Dong Chen,Muhammad Danang Birowosuto,Diing Shenp Ang,Chenjie Gu
Active control the surface-enhanced Raman scattering (SERS) enhancement shows great potential for realizing smart detection of different molecules. However, conventional methods usually involve time-consuming structural design or sophisticated fabrication process. Herein, we reported an electrically tunable field effect transistor (FET) comprised by WOx/MoOx hybrid as the SERS active layer. In the experiment, WOx/MoOx hybrid were first prepared by mixing different molar ratios of WOx and MoOx oxides. Then, R6G molecules was used as the Raman reporter, it showed that the intensity of the SERS signal observed on the most optimal hybrids (molar ratio = 1:3) could be promoted by two times as high as that observed on a single WOx or MoOx based substrate, which was ascribed to enhanced charge transfer efficiency by the constructed nano-heterojunction between the WOx and MoOx oxides. Thereafter, a back-gate FET was fabricated on a SiO2/Si substrate, and the most optimal WOx/MoOx hybrid was deposited as the gate channel as well as the SERS active layer. After that, a series of gate bias (from -15 V to 15 V) were implemented to actively tune the SERS performance of the FET. It evidenced that SERS EF can be further tuned from 2.39 × 107 (-15 V) to 6.55 × 107 (+10 V), which is ~7.4 / 4.1 times high than that observed on the pure WOx device (8.81 × 106) or pure MoOx (1.61 × 107) device, respectively. Finally, the mechanism behind the electrical tuning strategy was investigated. It revealed that a positive voltage would bend the conduction band down, which raised the electron density near the Fermi level. Consequently, it triggered the resonance charge transfer and significantly improved the SERS performance. On the contrary, a negative gate voltage attracted the holes to the Fermi level, which deferred the charge transfer process, and caused the degradation of the SERS enhancement.
研究领域:
材料科学
·
物理化学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL MICRODEVICES
·
2023
·Vol. 25
·期 3
·1-9
·
IF 2.8
Cao Xiaona,Hu Xiaoping,Qiu Ziyi,Xu Ting,Yu Zhenhua,Li Zhe,Jin Huawei,Xu Bingzhe
Early diagnosis of Alzheimer’s disease (AD) is critical for preventing disease progression, however, the diagnosis of AD remains challenging for most patients due to limitations of current sensing technologies. A common pathological feature found in AD-affected brains is the accumulation of Amyloid-β (Aβ) polypeptides, which lead to neurofibrillary tangles and neuroinflammatory plaques. Here, we developed a portable ultrasensitive FET biosensor chip based on a self-assembled nanoporous membrane for ultrasensitive detection of Aβ protein in complex environments. The microscale semiconductor channel was covered with a self-assembled organic nanoporous membrane modified by antibody molecules to pick up and amplify the Aβ protein signal. The nanoporous structure helps protect the sensitive channel from non-target proteins and improves its stability since no chemical functionalization process involved, largely reduces background noise of the sensing platform. When a bio-gated target is captured, the doping state of the polymer bulk could be tuned and amplified the strength of the weak signal, achieving ultrasensitive detecting performance (enabling the device to detect target protein less than 1 fg/ml in 1 µl sample). Moreover, the device simplifies the circuit connection by integrating all the connections on a 2 cm × 2 cm chip, avoiding expensive and complex manufacturing processes, and makes it usable for portable prognosis. We believe that this ultrasensitive, portable, low-cost Aβ sensor chip shows the great potential in the early diagnosis of AD and large-scale population screening applications.
研究领域:
生物传感器
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神经科学
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医学诊断
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纳米技术
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Yaping Xia,Yang Sun,Huamin Li,Shuo Chen,Tiying Zhu,Guangcan Wang,Baoyuan Man,Jie Pan,Cheng Yang
Room-temperature plasma treated graphene based FET was firstly proposed for the DNA hybridization detection. Affinity and electrical properties of the graphene based DNA-FET sensor were studied and improved benefits from the surface modification. The facile room-temperature Ar plasma easily removes residues from the graphene surface and changes the hydrophilic properties of graphene, which is important for our solution gated DNA-FET sensor. Limit of the detection of below 10 aM is obtained in our experiment. Especially, DNA concentration (C DNA )/the amount of net drain current (ΔI) and the negative shift in the V CNP value of the GFET sensor with the plasma treated 30 s are all improved compared with that without treatment. It shows that the easily plasma treatment of the graphene surface can be used for the solution gated FET sensor.
研究领域:
材料科学
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生物传感器
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纳米技术
·
电子工程
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yuqing Li,Bohao Liu,Yong Zhang
FET-type sensors have attracted considerable attention in trace-level hazardous analytes detection based on signal amplification effect. However, most of these sensors with either sensing gate or sensing channel are susceptible to humidity and signal crosstalk, significantly weakening the sensing performance. Herein, enlightened by the regulation of channel carriers attracted by ions from electric double layer (EDL), a side-gate FET (S-FET) gas sensor with ionogel dielectric sensing layer is rationally designed for proving the feasibility of novel capacitance sensing mode. The side-gate structure adequately exposes the dielectric sensing layer to the target gas and protects the channel region from being directly contacted to the ambient environment, guaranteeing the electrical and sensing stability of the sensor. Meanwhile, the gas volumetric adsorption nature of ionogel directly regulates the migration of ion carriers, macroscopically manifests the changes of EDL capacitance and ultimately influences the channel current of FET. The proposed FET sensor achieves excellent room-temperature gas sensing performances toward 0.02 ∼ 10 ppm of H 2 S, demonstrating an exceptional capability in food spoilage monitoring/halitosis prediagnosis. This work proposes a novel working mode of FET-type gas sensor for extending the categories of FET sensors, and offers relevant design concept in capacitance modulation for amplifying trace-level analytes-induced signal.
研究领域:
传感器与传感技术
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电子器件
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气体检测
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纳米电子学
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材料科学
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产品关联
DOI
高影响因子
Small
·
2022
·Vol. 18
·期 20
·2107650
·
IF 15.153
Yin Xia,Lingyi Zong,Yu Pan,Xinyu Chen,Lihui Zhou,Yiwen Song,Ling Tong,Xiaojiao Guo,Jingyi Ma,Saifei Gou,Zihan Xu,Sheng Dai,David Wei Zhang,Peng Zhou,Yu Ye,Wenzhong Bao
Two-dimentional semiconductors have shown potential applications in multi-bridge channel field-effect transistors (MBC-FETs) and complementary field-effect transistors (C-FETs) due to their atomic thickness, stackability, and excellent electrical properties. However, the exploration of MBC-FET and C-FET based on large-scale 2D semiconductors is still lacking. Here, based on a reliable vertical stacking of wafer-scale 2D semiconductors, large-scale MBC-FETs and C-FETs using n-type MoS 2 and p-type MoTe 2 are successfully fabricated. The drive current of an MBC-FET with two layers of MoS 2 channel (20 µm/10 µm) is up to 60 µA under 1 V bias. Compared with the single-gate MoS 2 FET, the carrier mobility of MBC-FET is 2.3 times higher and the sub-threshold swing is 70% smaller. Furthermore, NAND and NOR logic circuits are also constructed based on two vertically stacked MoS 2 channels. Then, C-FET arrays are fabricated by 3D integrating n-type MoS 2 FET and p-type MoTe 2 FET, which exhibit a voltage gain of 7 V/V when V DD = 4 V. In addition, this C-FET device can directly convert light signals to an electrical digital signal within a single device. The demonstration of MBC-FET and C-FET based on large-scale 2D semiconductors will promote the application of 2D semiconductors in next-generation circuits.
研究领域:
电子器件
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
·
IF 4.6
Zhong-Yan Xu,Li-Qiu Huang,Saima Rafique,Tian-Run Zhang,Hasiyati Duman,Ri-Jian Mo,Zhong-Qiu Li,Xing-Hua Xia
Spatiotemporal monitoring of reduced nicotinamide adenine dinucleotide (NADH) is critical for understanding cellular bioenergetics and redox homeostasis. However, conventional electrochemical methods are hindered by electrode fouling, interference, and a lack of spatial resolution required for subcellular analysis. Here, we present a “needle-type”, nanosized field-effect transistor (nano-FET) probe constructed on dual-barrel carbon nanoelectrodes for ultrasensitive NADH detection. The device features a nanoscopic polypyrrole (PPy) channel, modified with 1-methoxy-5-methylphenazinium methyl sulfate (mPMS) as a specific redox mediator. We establish a sensing mechanism based on coupled electron and ion transport, where mPMS-mediated NADH oxidation triggers ion dedoping in PPy channel to achieve intrinsic signal amplification. The nano-FET probe exhibits an exceptionally low NADH detection limit of 0.1 pM and a broad dynamic range (1 pM to 100 μM), accompanied by robust operational stability. This work significantly expands the toolkit of nanopipette-based electrochemical sensors, offering a powerful platform for spatially resolved metabolic profiling at the single-cell level.
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DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Qiongdi Zhang,Yuxuan Hao,Tonghua Zeng,Weiliang Shu,Pan Xue,Yang Li,Chi Huang,Liwei Ouyang,Xuming Zou,Zhen Zhao,Jiahong Wang,Xue-Feng Yu,Wenhua Zhou
Graphene field-effect transistors (GFETs) are widely used in biosensing due to their excellent properties in biomolecular signal amplification, exhibiting great potential for high-sensitivity and point-of-care testing in clinical diagnosis. However, difficulties in complicated fabrication steps are the main limitations for the further studies and applications of GFETs. In this study, a modular fabrication technique is introduced to construct microfluidic GFET biosensors within 3 independent steps. The low-melting metal electrodes and intricate flow channels are incorporated to maintain the structural integrity of graphene and facilitate subsequent sensing operations. The as-fabricated GFET biosensor demonstrates excellent long-term stability, and performs effectively in various ion environments. It also exhibits high sensitivity and selectivity for detecting single-stranded nucleic acids at a 10 f m concentration. Furthermore, when combined with the CRISPR/Cas12a system, it facilitates amplification-free and rapid detection of nucleic acids at a concentration of 1 f m . Thus, it is believed that this modular-fabricated microfluidic GFET may shed light on further development of FET-based biosensors in various applications.
研究领域:
生物传感器
·
临床诊断
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材料科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Nano
·
2024
·
IF 8.2
Hao Wang,Siyu Hou,Weihao Feng,Dongliang Li,Jialin Liu,Weisong Yang,Suichu Huang,Feiran Li,Xuezeng Zhao,Fang Chen,Cong Huang,Yunlu Pan
Biosensors based on field-effect transistor (FET) are extensively utilized in biomedical engineering for their capability to achieve rapid and label-free detection of targets. However, the extremely low concentration level of biomarkers, crucial for human health, put forward higher requirements for biosensors. Developing stable and reliable biosensors with high sensitivity for biomarker detection remains challenging. In this study, we report a highly sensitive MoS 2 -FET biosensor for the cytokine IFN-γ. Surface etching of the MoS 2 channel using a reactive ion etching system increases reactive sites for biological molecules on the channel surface, thereby enhancing the detection performance of the biosensor. The MoS 2 -FET biosensor functionalized with aptamer demonstrates high sensitivity and selectivity for IFN-γ detection (limit of detection is 5.98×10 -5 nM). Notably, the signal responses of the MoS 2 -FET biosensor are higher than that of the MoS 2 -FET biosensor without Ar etching and graphene-FET biosensor. Additionally, this paper analyzes the relationship between the modification density of probe molecules and the biosensor's detection signal, establishing a theoretical foundation for further enhancing the sensor's detection performance. Experimental results confirm that the highly sensitive MoS 2 -FET biosensor provides an effective testing platform for the biomarkers with low concentrations, crucial for disease prevention and early diagnosis.
研究领域:
生物医学工程
·
纳米技术
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yi Yu,Chunzi Liang,Qiang-Qiang Wan,Dan Jin,Xi Liu,Zhiyong Zhang,Zhong-Yue Sun,Guo-Jun Zhang
Tumor-derived exosome (TD-Ex) serves as a crucial early diagnostic biomarker of pancreatic cancer (PC). However, accurate identification of TD-Ex from PC is still a challenging work. In this paper, a detection microsystem that integrates magnetic separation and FET biosensor is developed, which is capable of selectively separating TD-Ex of PC from the plasma and detecting exosomal miRNA10b in a sensitive and specific manner. The magnetic beads were functionalized with dual antibody (GPC-1 antibody and EpCAM antibody), enabling selective recognition and capture of PC-derived exosomes. On the other hand, a peptide nucleic acid (PNA)- functionalized reduced graphene oxide field-effect transistor (RGO FET) biosensor was subsequently utilized to detect the exosomal miRNA10b, which is highly expressed in PC- derived exosomes. This system could achieve a low detection limit down to 78 fM, and selectively identify miRNA10b from single-base mismatched miRNA . In addition, 40 clinical plasma samples were tested with this microsystem, and the results indicate that it could effectively distinguish PC patients from healthy individuals. The assay combines specific capture and enrichment of PC-derived exosomes with sensitive and selective detection of exosomal miRNA, showing its potential to be used as an effective scheme for PC early diagnosis.
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 2
·226
·
IF 4.4
Xinhao Zhang,Shuo Chen,Heqi Ma,Tianyu Sun,Xiangyong Cui,Panpan Huo,Baoyuan Man,Cheng Yang
Field-effect transistor (FET) biosensors can be used to measure the charge information carried by biomolecules. However, insurmountable hysteresis in the long-term and large-range transfer characteristic curve exists and affects the measurements. Noise signal, caused by the interference coefficient of external factors, may destroy the quantitative analysis of trace targets in complex biological systems. In this report, a “rectified signal” in the output characteristic curve, instead of the “absolute value signal” in the transfer characteristic curve, is obtained and analyzed to solve these problems. The proposed asymmetric Schottky barrier-generated MoS2/WTe2FET biosensor achieved a 105rectified signal, sufficient reliability and stability (maintained for 60 days), ultra-sensitive detection (10 aM) of the Down syndrome-related DYRK1A gene, and excellent specificity in base recognition. This biosensor with a response range of 10 aM–100 pM has significant application potential in the screening and rapid diagnosis of Down syndrome.Keywords:Schottky junction;field-effect transistor;biosensor;rectified signal;ultra-sensitive detection
研究领域:
材料科学
·
生物医学工程
·
基因检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）