RSC Advances
·
2025
·Vol. 15
·期 36
·29300-29310
·
IF 4.6
Xiao Zhou,Huali Huang,Lifeng Luo,Chenxi Cui,Hongmian Jiang
Pancreatic cancer, a highly aggressive solid tumor, presents multiple challenges in treatment. Although surgical resection is the primary treatment for early-stage non-metastatic cases, the deep abdominal location of the pancreas complicates precise intraoperative localization, adversely affecting surgical outcomes and patient prognosis. To address these clinical challenges, we aim to develop an innovative intraoperative navigation platform for precision tumor-targeted delivery. We systematically evaluated the tumor-targeting specificity and tissue-penetrating capacity of three hydrosoluble polymers, i.e., dextran, PEG, and HA, in pancreatic carcinoma models. The uptake kinetics of these materials in cancer cells and their penetration dynamics across complex tumor matrices were quantitatively evaluated via in vitro assays and three-dimensional tumor spheroid models. Complementary in vivo validation involved the use of PNAC-1 cell subcutaneous xenograft models to map the spatiotemporal distribution and tumoral accumulation of the lead candidates. Results demonstrated that dextran-FITC had a superior tumor-targeting performance, showing a significantly higher fluorescence intensity than PEG-FITC and HA-FITC. These preliminary data reveal significant heterogeneity in the intertumoral localization efficiency among the polymer carriers, establishing a mechanistic foundation for intraoperative navigation. Through rational optimization of carrier selection and delivery modalities, this platform aims to enhance surgical precision, minimize off-target effects, and ultimately improve clinical outcomes.
研究领域:
肿瘤学
·
外科研究
·
生物医学工程
·
分子成像
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Yanan Zhang,Dajun Hou,Xueli Yu
A ratiometric fluorescent pH sensor was facilely constructed by covalent modification of amino-terminated silicon nanodots (SiND) with pH-sensitive fluorescein isothiocyanate (FITC). After optimization, the SiND-FITC(40:1) material with a SiND:FITC initial mass ratio of 40:1 was selected for the sensing of hydrogen ions. It was observed that the material inherits the unique features of SiND and FITC, and there is significant improvement of SiND acid−base stability, which is a favorable factor in terms of providing fluorescence reference signal. The SiND-FITC(40:1) material displays not only high pH sensitivity, but also good stability and anti-interference ability, and the response process is highly reversible. Deploying the SiND-FITC(40:1) material, we have made available a simple, sensitive, and precise approach for pH sensing. In aqueous solutions, the I 517 /I 466 fluorescence intensity ratio of SiND-FITC(40:1) increases linearly in the pH range of 5.40–7.76. This dual emission nanosensor was successfully applied for pH sensing and cellular fluorescence imaging.
研究领域:
纳米技术
·
材料科学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xueyan Yun,Shuai Yuan,Shuo Shi,Yangyang Wang,Linze Liu,Lijun Song,Tungalag Dong
Amine-responsive fluorescent indicator films for meat freshness were constructed by incorporating β-glucan grafted with fluorescein isothiocyanate (FITC) in polyvinyl alcohol (PVA). The structural, thermal, and responsive properties of grafted copolymers (βGlu-FITC) were investigated. The results of 1 H NMR spectra showed that βGlu-FITC exhibited proton signals between 6.5 and 6.7 ppm, suggesting the successful grafting of FITC onto β-glucan. The fluorescence quantum yield of βGlu-FITC was 18.72%, which was higher than that of β-glucan of 12.94% at the optimum excitation wavelength of ultraviolet light of 365 nm. With the incorporation of PVA as a film-forming matrix, βGlu-FITC/PVA films showed high yellow-green fluorescent responsiveness under volatile ammonia and pH solution. When the film was used for meat freshness, the 0.06βGlu-FITC/PVA film provided a noticeable fluorescent indication of pork freshness that corresponded to increases in total volatile basic nitrogen, total colony counts, and drip loss, suggesting its potential for meat packaging and as a freshness indicator.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2021
·
IF 4.833
Xinyue Chen,Dan Liu,Sanhu Gou,Jiahui Ji,Zhiyuan Xue,Shilan Feng
A colorimetric assay based on the oriented TA-AuNPs-PEG-FITC for rapid and sensitive PABA detection was proposed. Initially, the negatively charged TA modified on the surface of AuNPs enabled rapid binding to PABA due to the electrostatic interaction and hydrogen bond, resulting in a visible color change. However, particles were aggregated in a non-oriented way, leading to an unstable testing system which failed to realize the accurate quantification. Thus, the asymmetrically functionalized TA-AuNPs-PEG-FITC were prepared, in which the stabilizer agent of HS-PEG-FITC was attached to the specific sites on the surface of TA-AuNPs. Such a way of modification resulted in an oriented aggregation manner, the addition of PABA induced the formation of oligomers. Moreover, the introduction of FITC group acted as fluorescent marker, providing a simple, fast and quantitative method for characterization of PEG chain. The molar ratio between TA-AuNPs and modified PEG-FITC was further determined. A linear regression was established in a wide range from 10 μM to 1 mM with the LOD of 6.9 μM. Finally, the sensor was successfully applied in the health food. This was the first example in which TA-AuNPs-PEG-FITC were fabricated for colorimetric detection of PABA, and an indirect method based on fluorescence marker was well used to quantify the content of PEG chain.
研究领域:
分析化学
·
纳米生物学
·
食品科学
✓ 已验证
DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 1
·127-138
·
IF 5.068
Deng Guoying,Wu Zizheng,Zhou Feng,Dai Chenyun,Zhao Jingpeng,Kang Yingjie,Wang Qian,Liu Xijian,Wang Yin,Wang Qiugen
Drug delivery system studies aim to improve nanoparticle (NP) formulation to enable efficient delivery of NPs to tumors. However, NPs must be transported by blood or through direct injection. How NPs leave the circulatory system and how NPs diffuse into a tumor remain unclear, and this
uncertainty is a limitation of drug delivery systems. The intimate connection between these questions and metabolism may be related to their biosafety in vivo. Thus, in this study, classical carrier SiO2 NPs were used as typical transport NPs, and fluorescein isothiocyanate
(FITC) was used as the representative drug and tracer. As exosome and tunneling nanotubes (TNTs) are the most relevant mechanism for NP transportation and considering the local situation in a tumor, we focused on identifying this phenomenon and investigating TNTs. In conclusion, we effectively
demonstrated that NPs can be transferred from cell to cell. Nanotubes may play an important role in this process.
研究领域:
纳米技术
·
癌症研究
·
药物递送系统
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 94
·51950-51959
·
IF 3.708
Jie Feng,Di Chang,Zhifei Wang,Bin Shen,Jinjin Yang,Yanyun Jiang,Shenghong Ju,Nongyue He
Dual-modal imaging contrast agents with unique X-ray computed tomography (CT) and optical imaging capabilities have attracted much attention in recent years. Herein, the new silica hybrid nanocomposites containing Au nanoparticles and FITC dyes (FITC–Au@SiO2) were investigated as this kind of contrast agent. TEM characterization showed that the as-synthesized FITC–Au@SiO2 nanoprobes were uniform in morphology with the average size of 90 nm. After the PEGylation, the obtained FITC–Au@SiO2@PEG exhibited good dispersion stability under different conditions. The MTT assay suggested that FITC–Au@SiO2@PEG didn't show appreciable toxicity toward KB cells even at high concentration of up to 1000 μg mL−1. In addition, it was found that FITC–Au@SiO2@PEG could cross the cell membrane and enter into the cytoplasm during the incubation with cells. In vivo dual modal optical/CT imaging of C57BL/6J mice uncovered that within 2 h post-injection FITC–Au@SiO2@PEG nanoprobes preferred to accumulate in the liver and were gradually cleared through the enterohepatic circulation system, which could bring novel opportunities to the next generation of dual-modality contrast agents.
研究领域:
纳米技术
·
生物医学工程
·
医学成像
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Ying Zhou,Jie Ding,Tingxizi Liang,E. S. Abdel-Halim,Liping Jiang,Jun-Jie Zhu
Fluorescent nanosensors have been widely applied in recognition and imaging of bioactive small molecules; however, the complicated surface modification process and background interference limit their applications in practical biological samples. Here, a simple, universal method was developed for ratiometric fluorescent determination of general small molecules. Taking superoxide anion (O2•–) as an example, the designed sensor was composed of three main moieties: probe carrier, rattle-type silica colloidal particles (mSiO2@hmSiO2 NPs); reference fluorophore doped into the core of NPs, fluorescein isothiocyanate (FITC); fluorescent probe for superoxide anion, hydroethidine (HE). In the absence of O2•–, the sensor just emitted green fluorescence of FITC at 518 nm. When released HE was oxidized by O2•–, the oxidation product exhibited red fluorescence at 570 nm and the intensity was linearly associated with the concentration of O2•–, while that of reference element remained constant. Accordingly, ratiometric determination of O2•– was sensitively and selectively achieved with a linear range of 0.2–20 μM, and the detection limit was calculated as low as 80 nM. Besides, the technique was also successfully applied for dual-emission imaging of O2•– in live cells and realized visual recognition with obvious fluorescence color change in normal conditions or under oxidative stress. As long as appropriate reference dyes and sensing probes are selected, ratiometric biosensing and imaging of bioactive small molecules would be achieved. Therefore, the design could provide a simple, accurate, universal platform for biological applications.
研究领域:
分析化学
·
生物技术
·
生物化学
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2026
·
IF 3.6
Wan Jiahao,Wang Yanbin,Deng Wenhui,Liu Caijun,Liu Menglong,Luo Denglin,Zhang Kangyi,Gao Haiyan,Bai Zhouya
Fluorescent labeling of long-chain inulin (FXL) is essential for tracking its spatiotemporal dynamics in gastrointestinal systems, yet conventional methods risk altering its native properties. This study systematically compared three labeling strategies: FITC-DB (dibutyltin dilaurate-catalyzed hydroxyl conjugation), FITC-RA (reductive amination), and FA (fluoresceinamine coupling). Multimodal characterization revealed that FITC-DB achieved the highest substitution degree (DS = 0.42 at 50 mg FITC), though fluorescence quenching occurred at elevated loadings. FITC-RA induced significant aggregation (peak size at 50 mg), while FA labeling exhibited minimal structural perturbation but low efficiency (DS < 0.20). Spectroscopic analyses confirmed covalent thiourea bond formation (1720 cm⁻¹ FTIR peak) and aggregation-dependent optical shifts. Crucially, FITC-DB at 50 mg balanced optimal labeling efficiency, colloidal stability (highest zeta potential), and preserved macromolecular architecture. These findings establish design principles for high-fidelity FXL probes in biological tracking applications.
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Yingjie Yuan,Xiangzhen Chen,Lei Jia,Yongxin Li,Dan Zhao,Lina Zhang,Yuanyuan Huang,Jun Xu,Tongqian Zhao
Long-term discharge of tetracycline (TC) in the environment exerts selective pressure on the bacteria, increasing resistance in sensitive bacteria and posing a serious threat to public health. Therefore, it is crucial to develop real-time detection of tetracycline. In this study, we reported a novel rare-earth fluorescent nanosensor FITC-GMP-Eu, based on the formation of guanosine-5′-monophosphate disodium salt (GMP) and fluorescein isothiocyanate (FITC), which enabled the real-time identification of tetracycline in the environment. When there was no TC in the environmental sample, the FITC-GMP-Eu nanosensor exhibited the green fluorescence of FITC due to the fluorescence quenching effect of water molecules on Eu 3+ . When the environmental samples contained TC, the red fluorescence of Eu 3+ could be sensitized due to the diketone structure in the tetracycline molecule, which enabled the naked-eye visualized identification of TC. The nanosensor had high sensitivity (detection limit of 19 nM) and a wide detection range (0–30 μM) for TC. At the same time, the sensor was characterized by high visualization and anti-interference ability due to the strong fluorescence of both FITC and Eu complexes. In order to improve the practicality of the detection, this study also prepared a paper-based and sodium alginate gelated fluorescence detection platform with low background fluorescence. Combined with the common color analysis and RGB extraction procedures in mobile phones, real-time and on-site identification of TC on the surface of various fruit samples can be achieved.
研究领域:
环境科学
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2016
·Vol. 8
·期 9
·5089-5097
·
IF 7.76
Abdul Hakeem,Fouzia Zahid,Ruixue Duan,Muhammad Asif,Tianchi Zhang,Zhenyu Zhang,Yong Cheng,Xiaoding Lou,Fan Xia
Herein, we design novel cellulose conjugated mesoporous silica nanoparticle (CLS-MSP) based nanotherapeutics for stimuli responsive intracellular doxorubicin (DOX) delivery. DOX molecules are entrapped in pores of the fabricated mesoporous silica nanoparticles (MSPs) while cellulose is used as an encapsulating material through esterification on the outlet of the pores of the MSPs to avoid premature DOX release under physiological conditions. In in vitro studies, stimuli responsive DOX release is successfully achieved from DOX loaded cellulose conjugated mesoporous silica nanoparticles (DOX/CLS-MSPs) by pH and cellulase triggers. Intracellular accumulation of DOX/CLS-MSPs in human liver cancer cells (HepG2 cells) is investigated through confocal microscope magnification. Cell viability of HepG2 cells is determined as the percentage of the cells incubated with DOX/CLS-MSPs compared with that of non-incubated cells through an MTT assay.
研究领域:
药剂学
·
生物材料
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2013
·
IF 2.108
Bihua Xia,Fang He,Lidong Li
A simple strategy to prepare aggregated Ag nanoparticle composites in solution by self-assembly is developed. Sodium citrate is used as the reduction agent to produce negatively charged Ag nanoparticles which are then aggregated using cationic polyacrylamide (PAM) to form self-assembled Ag-PAM microcomposites. In these microcomposites, the degree of aggregation of Ag nanoparticles can be controlled by changing the Ag/PAM ratio. This special structural feature can be applied in the study of metal-enhanced fluorescence. Confocal laser scanning microscopy and time-resolved fluorescence measurements are used to examine the fluorescence enhancement phenomenon of the Ag-PAM microcomposites. The microcomposites considerably enhanced the fluorescence intensity of fluorescein isothiocyanate isomer (FITC).
研究领域:
材料科学
·
光学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Ling Chen,Jinhua Li,Shasha Wang,Wenhui Lu,Aiguo Wu,Jaebum Choo,Lingxin Chen
A sensing strategy for chromogenic detection of molecular biothiols has been proposed based on fluorescein isothiocyanate (FITC) functionalized magnetic core–shell Fe 3 O 4 /Ag hybrid nanoparticles. Ag coated magnetic Fe 3 O 4 nanoparticles were initially synthetized. FITC was subsequently conjugated on the surface of core–shell nanoparticles by Ag–SCN linkage and then the fluorescence of FITC was quenched. Upon addition of molecular biothiols, since the Ag S bond is stronger than Ag–SCN, a place-displacement between thiols and FITC would occur and thereby the fluorescence of FITC would recover. Thus, a fluorescence “off-on” probe was attained by virtue of biothiols, so after magnetic separation, the fluorescence signal change of FITC in clear solution could be employed for quantitative determination of typical molecular biothiols such as glutathione (GSH) and cysteine (Cys). High sensitivity was obtained with the detection limits of 10 nM and 20 nM for GSH and Cys, respectively. As well as, the assay strategy presented excellent selectivity toward molecular biothiols against other amino acids. Furthermore, confocal imaging was achieved in living cells, indicating that this fluorescent probe is potentially applicable for imaging molecular thiols in biological systems.
研究领域:
分析化学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）