JOURNAL OF NANOBIOTECHNOLOGY
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2021
·Vol. 19
·期 1
·1-14
·
IF 10.435
Xiong Wei,Lan Qiumei,Liang Xiaonan,Zhao Jinmin,Huang Hanji,Zhan Yanting,Qin Zainen,Jiang Xianfang,Zheng Li
Intra-articular (IA) injection is an efficient treatment for osteoarthritis, which will minimize systemic side effects. However, the joint experiences rapid clearance of therapeutics after intra-articular injection. Delivering system modified through active targeting strategies to facilitate localization within specific joint tissues such as cartilage is hopeful to increase the therapeutic effects. In this study, we designed a nanoscaled amphiphilic and cartilage-targeting polymer-drug delivery system by using formononetin (FMN)-poly(ethylene glycol) (PEG) (denoted as PCFMN), which was prepared by PEGylation of FMN followed by coupling with cartilage-targeting peptide (CollBP). Our results showed that PCFMN was approximately regular spherical with an average diameter about 218 nm. The in vitro test using IL-1β stimulated chondrocytes indicated that PCFMN was biocompatible and upregulated anabolic genes while simultaneously downregulated catabolic genes of the articular cartilage. The therapeutic effects in vivo indicated that PCFMN could effectively attenuate the progression of OA as evidenced by immunohistochemical staining and histological analysis. In addition, PCFMN showed higher intention time in joints and better anti-inflammatory effects than FMN, indicating the efficacy of cartilage targeting nanodrug on OA. This study may provide a reference for clinical OA therapy.
研究领域:
药剂学
·
纳米技术
·
骨科
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
Cell Reports Physical Science
·
2020
·
IF 0
Heping Xie,Wenchuan Jiang,Tao Liu,Yifan Wu,Yufei Wang,Bin Chen,Dawen Niu,Bin Liang
Carbon capture and storage, in which CO 2 is selectively removed and sequestrated from flue gas, is a promising strategy to mitigate CO 2 emissions and global warming. Conventionally, CO 2 capture involves an energy-consuming absorbent regeneration step by thermal decomposition (2.0–4.0 GJ ton −1 CO 2 ), which accounts for the majority (60%–70%) of the total cost. Here, we propose an alternative electrochemical cycle to capture CO 2 in an aqueous alkaline solution, facilitated by the pH swing effect from a proton-coupled electron transfer redox reaction of a biological proton carrier, riboflavin 5′-monophosphate sodium salt hydrate (FMN/FMNH 2 ). Under lab conditions, an electrolysis cell implementing the FMN proton carrier demonstrates a high energy efficiency, with only 9.8 kJ mol −1 CO 2 captured, which is much lower than traditional approaches using a monoethanolamine absorbent (2.0–4.0 GJ ton −1 CO 2 , 88–176 kJ mol −1 CO 2 ). Thus, this system may contribute toward lowering the cost of CO 2 capture.
研究领域:
环境科学
·
生物化学
✓ 已验证
DOI
ENZYME AND MICROBIAL TECHNOLOGY
·
2019
·
IF 3.553
Fei-Long Li,Wen Bin Su,Qing-Lan Tao,Liao-Yuan Zhang,Ye-Wang Zhang
Enzyme-catalyzed cofactor regeneration is a significant approach to avoid large quantities consumption of oxidized cofactor, which is vital in a variety of bioconversion reactions. NADH: FMN oxidoreductase is an ideal regenerating enzyme because innocuous molecular oxygen is required as an oxidant. But the by-product H 2 O 2 limits its further applications at the industrial scale. Here, novel NADH: FMN oxidoreductase (LrFOR) from Lactobacillus rhamnosus comprised of 1146 bp with a predicted molecular weight of 42 kDa was cloned and overexpressed in Escherichia coli BL21 (DE3). Enzyme assay shows that the purified recombinant LrFOR has both the NADPH and NADH oxidation activity. Biochemical characterizations suggested that LrFOR exhibits the specific activity of 39.8 U·mg −1 with the optimal pH and temperature of 5.6 and 35 °C and produces H 2 O instead of potentially harmful peroxide. To further study its catalytic function, a critical Thr29 residue and its six mutants were investigated. Mutants T29G, T29A, and T29D show notable enhancement in activities compared with the wild type. Molecular docking of NADH into wild type and its mutants reveal that a small size or electronegative of residue in position29 could shorten the distance of NADH and FMN, promoting the electrons transfer and resulting in the increased activity. This work reveals the pivotal role of position 29 in the catalytic function of LrFOR and provides effective catalysts in NAD + regeneration.
研究领域:
生物化学
·
酶学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2024
·
IF 4.4
Bing Fu,Meng Chen,Xianfeng Bao,Jiajie Lu,Zhiwen Zhu,Fuyao Guan,Chuyang Yan,Peize Wang,Linglin Fu,Ping Yu
Vitamin B 2 is an essential water-soluble vitamin. For most prokaryotes, a bifunctional enzyme called FAD synthase catalyzes the successive conversion of riboflavin to FMN and FAD. In this study, the plasmid pNEW-AZ containing six key genes for the riboflavin synthesis was transformed into strain R2 with the deleted FMN riboswitch, yielding strain R5. The R5 strain could produce 540.23 ± 5.40 mg/L riboflavin, which was 10.61 % higher than the R4 strain containing plasmids pET-AE and pAC-Z harboring six key genes. To further enhance the production of riboflavin, homology matching and molecular docking were performed to identify key amino acid residues of FAD synthase. Nine point mutation sites were identified. By comparing riboflavin kinase activity, mutations of T203D and N210D, which respectively decreased by 29.90 % and 89.32 % compared to wild-type FAD synthase, were selected for CRISPR/Cas9 gene editing of the genome, generating engineered strains R203 and R210. pNEW-AZ was transformed into R203, generating R6. R6 produced 657.38 ± 47.48 mg/L riboflavin, a 21.69 % increase compared to R5. This study contributes to the high production of riboflavin in recombinant E. coli BL21.
研究领域:
生物工程
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yidong Zhou,Zhijian Ke,Hangting Ye,Mengting Hong,Yifei Xu,Mingliang Zhang,Wankui Jiang,Qing Hong
Carbaryl is a widely used carbamate pesticide in agriculture. The strain Rhizobium sp. X9 possesses the typical carbaryl degradation pathway in which carbaryl is mineralized via 1-naphthol, salicylate, and gentisate. In this study, we cloned a carbaryl hydrolase gene cehA and a novel two-component 1-naphthol hydroxylase gene cehC1C2. CehA mediates carbaryl hydrolysis to 1-naphthol and CehC1, an FMNH2 or FADH2-dependent monooxygenase belonging to the HpaB superfamily, and hydroxylates 1-naphthol in the presence of reduced nicotinamide-adenine dinucleotide (FMN)/flavin adenine dinucleotide (FAD), and the reductase CehC2. CehC1 has the highest amino acid similarity (58%) with the oxygenase component of a two-component 4-nitrophenol 2-monooxygenase, while CehC2 has the highest amino acid similarity (46%) with its reductase component. CehC1C2 could utilize both FAD and FMN as the cofactor during the hydroxylation, although higher catalytic activity was observed with FAD as the cofactor. The optimal molar ratio of CehC1 to CehC2 was 2:1. The Km and Kcat/Km values of CehC1 for 1-naphthol were 74.71 ± 16.07 μM and (8.29 ± 2.44) × 10–4 s–1·μM–1, respectively. Moreover, the enzyme activities and substrate spectrum between CehC1C2 and previously reported 1-naphthol hydroxylase McbC were compared. The results suggested that McbC had a higher 1-naphthol hydroxylation activity, while CehC1C2 had a broader substrate spectrum.
研究领域:
环境微生物学
·
生物化学
·
农药生物降解
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY
·
2025
·
IF 9.4
Qing Li,Yifan Wu,Xinchen Song,Qihang He,Yunpeng Wang,Wenchuan Jiang,Cheng Lan,Zhiyu Zhao,Tao Liu,Heping Xie
CO 2 batteries offer a promising solution for mitigating climate change by reducing CO 2 emissions. Using ammonia, a highly effective gaseous alkali, we can harness chemical energy through its reaction with CO 2 for power generation. However, the lack of direct electron transfer in the ammonia-CO 2 carbonation reaction and ammonia's susceptibility to precious metal catalysis side reactions and corrosion complicate the construction of an efficient battery system. To overcome these challenges, we propose a concept that uses organic catalytic reactions to harness the chemical energy from the CO 2 -ammonia reaction for electricity generation. By employing isoalloxazine derivatives—riboflavin sodium phosphate (FMN-Na)—as proton carriers in redox reactions, we can create battery electromotive force through the proton concentration difference (ΔpH) between CO 2 and ammonia. This approach leverages reversible electrochemical proton-coupled electron transfer (PCET) reactions to drive electron flow and facilitate power generation, without requiring renewable electricity charging. Our study demonstrates that a CO 2 -ammonia battery can achieve a peak power density of 22.5 W.m -2 , converting 1 ton of CO 2 into 60.04 kWh of electricity while producing over 99.60% purity ammonium bicarbonate. Our work combines CO 2 mitigation, electricity generation, and the production of valuable by-products, offering innovative strategies for addressing environmental and energy challenges simultaneously.
研究领域:
能源化学
·
环境科学
·
电化学
·
可持续能源
·
二氧化碳利用
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2014
·Vol. 2
·期 44
·7756-7764
·
IF 0
Jiang Wu,Ying Liu,Wei Li,Chunyan Wang,Yanjun Li,Ying Tian,Jing Sun,Shouju Wang,Xin Wang,Yuxia Tang,Hong Zhu,Zhaogang Teng,Guangming Lu
Small interfering RNA (siRNA) has great potential for gene therapy of breast cancer. The development of multifunctional nanocarriers possessing the targeted ability and multimodal imaging function is important to increase siRNA transfection efficiency and to visualize the delivery events. Herein, we demonstrate flavin mononucleotide (FMN) and polyethylenimine (PEI) modified Fe3O4@mTiO2 nanoparticles (Fe3O4@mTiO2/FMN-PEI) as novel magnetic fluorescent siRNA delivery systems. The system has magnetic targeting, magnetic resonance imaging (MRI), optical imaging, and high siRNA binding ability. Survivin-siRNA delivered by this system can effectively enter into MCF-7 cells, which can be further enhanced by exerting an external magnetic field. More importantly, the siRNA delivery events including the location, trafficking, distribution, and release, can be visualized by MRI and optical imaging. Gene silencing by survivin-siRNA can cause significant protein knockdown (96%) and considerable cell apoptosis (30%), indicating the usefulness of Fe3O4@mTiO2/FMN-PEI as a siRNA delivery system.
研究领域:
癌症研究
·
基因治疗
·
纳米技术
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
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