SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Fanyong Yan,Zhangjun Bai,Yuan Chen,Fanlin Zu,Xing Li,Jinxia Xu,Liang Chen
A novel FRET ratiometric fluorescent probe (CMH-GA-CDs) was designed for the detection of Cu 2+ in aqueous solution. The CMH-GA-CDs were prepared through grafting 7-diethylaminocoumarin-3-carbohydrazide (HCM) on the surface of glyoxylic acid-modified carbon dots (GA-CDs) by amidation reaction. In the absence of Cu 2+ , a FRET pair from the CDs to CMH units was established, in which the CMH-GA-CDs exhibited dual emission bands centered at 400 nm (CDs) and 458 nm(CMH), respectively, under optimal excitation wavelength of CDs (λ ex = 340 nm). Upon addition of 30 μM Cu 2+ to CMH-GA-CDs solution, Cu 2+ coordinated with the heteroatoms N and O, which caused the inhibition of FRET process and the change of fluorescence intensity ratio I 458 /I 400 from 1.95 to 0.91. The ratio of I 458 /I 400 exhibited a good linear relationship with the concentration of Cu 2+ in the range of 0–10 μM and the limit of detection was as low as 0.21 μM. Furthermore, The CMH-GA-CDs were used for the detection of Cu 2+ in tap water and lake water with satisfactory recovery ratio between 91% and 104%, demonstrating the promising applicability for detecting Cu 2+ in water samples.
研究领域:
分析化学
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材料科学
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环境科学
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2025
·
IF 4.7
Bei Jiang,Jintao Jiang,Xinrui He,Yilei Zhu,Chao Guo
Accurate quantification of total antioxidant capacity (TAC) is crucial for evaluating the overall antioxidant activity in biological systems and food products. This is of growing importance in diverse applications such as health risk assessment and nutritional recommendations. Here, we introduce a novel Förster resonance energy transfer (FRET)-based sensor for the determination of TAC. The sensor utilizes an iodine-mediated FRET mechanism, employing BODIPY as the donor and Nile Red as the acceptor, both encapsulated within polystyrene (PS) microspheres. In the presence of iodine, its lipophilicity facilitates adsorption into the PS microspheres, resulting in fluorescence quenching due to the heavy-atom effect. This quenching results in an increased distance between the unquenched donors and acceptors, thereby decreasing FRET efficiency. Conversely, the presence of antioxidants reduces iodine concentration, which increases FRET efficiency. This dynamic allows for the development of a ratiometric fluorescence method for TAC determination. Critically, the sensor demonstrates exceptional photostability and resilience to variations in pH and ionic strength, contributing to its analytical robustness. The proposed method exhibits a linear response to ascorbic acid concentrations ranging from 0 to 50 μM, exhibiting good sensitivity (LOD = 2.5 μM) and selectivity. Additionally, the method has been successfully applied to evaluate TAC in ascorbic acid chewable tablets and lemon juice, demonstrating its potential as a robust and efficient tool for TAC determination.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-19
·
IF 15.7
Ren Tingju,Zhang Yingzhi,Tong Yuxiao,Zhang Qi,Wang Tianhao,Wang Yue,Yang Chunguang,Xu Zhangrun
Glycosylated RNAs (glycoRNAs), a recently discovered class of membrane-associated glyco-molecules, remain poorly understood in function and clinical value due to limited detection methods. Here, we show a dual recognition Förster resonance energy transfer (drFRET) strategy using nucleic acid probes to detect N-acetylneuraminic acid-modified RNAs, enabling sensitive, selective profiling of glycoRNAs on small extracellular vesicles (sEVs) from minimal biofluids (10 μl initial biofluid). Using drFRET, we identify 5 prevalent sEV glycoRNAs derived from 7 cancer cell lines. In a 100-patient cohort (6 cancer types and non-cancer controls), sEV glycoRNA profiles achieve 100% accuracy (95% confidence interval) in distinguishing cancers from non-cancer cases and 89% accuracy in classifying specific cancer types. Furthermore, drFRET reveal that sEV glycoRNAs specifically interact with Siglec proteins and P-selectin, which is critical for sEV cellular internalization. The drFRET strategy provides a versatile and sensitive platform for the imaging and functional analysis of sEV glycoRNAs, with promising implications for clinical applications.
研究领域:
分子生物学
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肿瘤学
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临床医学
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生物化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2014
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IF 2.129
Jingyu Piao,Jia Lv,Xin Zhou,Tong Zhao,Xue Wu
A novel fluorescent chemosensor bearing a rhodamine and a dansyl moiety was developed for highly selective detection of Fe 3+ based on fluorescence resonance energy transfer (FRET) mechanism. Binding of Fe 3+ to the chemosensor induced spirolactam ring opening in the rhodamine moiety and subsequent off–on FRET from the dansyl energy donor to the rhodamine energy acceptor due to the spectral overlap between the emission of the dansyl moiety and the absorption of the ring opened rhodamine moiety. Job’s plot analysis indicated a 1:1 binding stoichiometry between the chemosensor and Fe 3+ . The association constant was estimated to be 2.72 × 10 3 M −1 according to the Benesi–Hildebrand method. With the feature of easy synthesis, simple structural skeleton and excellent sensing ability, the newly synthesized chemosensor provided the potential for applying as a highly selective fluorescent probe in complex samples containing various competitive metal ions and developing other metal ion chemosensors to fulfill various needs of biological and environmental field.
研究领域:
分析化学
·
生物化学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Yang Shu,Na Zheng,An-Qi Zheng,Ting-Ting Guo,Yong-Liang Yu,Jian-Hua Wang
Fluorescence imaging of cellular metals is widely reported. However, the quantification of intracellular metals with fluorescence imaging is so far not feasible and highly challenging. In this work, a ratiometric probe with two fluorescently labeled complementary DNA strains is designed for intracellular zinc quantification via fluorescence imaging, based on fluorescence resonance energy transfer (FRET) from carbon dots (CDs) to fluorescein (FAM). The donor CDs are modified with a Zn2+ aptamer, whereas the receptor FAM is conjugated with the complementary DNA sequence to ensure selectivity. MCF-7 cells are cultured sequentially with Zn2+ (20, 40, 55, 70, 85, and 100 μmol L–1) and CDs–FAM (100 μg mL–1), which is used for fluorescence imaging (at λex = 405 nm and λem = 440–490 nm for CDs, λem = 500–550 nm for FAM) to provide a relative fluorescence ratio ((F – F0)/F0, F = ICDs/IFAM), followed by quantifying intracellular zinc with ICPMS. A linear correlation is achieved between the relative fluorescence ratio in fluorescence images and the intracellular zinc content derived by ICPMS, which facilitates intracellular zinc quantification via fluorescence imaging. It is especially useful for real-time tracing of intracellular zinc during the cell culturing process or in vivo. The cellular uptake of Zn2+ by MCF-7 cells is further evaluated with this approach by culturing with 100 μmol L–1 of Zn2+ for different times, and a maximum uptake of 60.5 fg per cell is observed at an incubation time of 60 min. This value is further demonstrated well by ICPMS detection.
研究领域:
分析化学
·
细胞生物学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2025
·
IF 4.9
Haoye Tian,Chennan He,Haiping Zhang,Xia Dong,Jinxin He,Jinfang Zhang,Qiangqiang Zhao
Phloxine B (PHB) demonstrates strong catalytic performance in cross-dehydrogenative coupling (CDC) reactions, yet its light-harvesting efficiency remains limited. Dye-supported nanofiber photocatalysts were developed via electrospinning, integrating polyvinyl alcohol (PVA), polydimethyldiallyl ammonium chloride (PDADMAC), and the Förster resonance energy transfer (FRET) pair: AY184 (donor) and PHB (acceptor). Four strategies—electrospinning-adsorption, blending-electrospinning, co-electrospinning from a single nozzle, and co-electrospinning from separate nozzles—were explored to optimize dye distribution and FRET efficiency. Among the synthesized nanofibers, the NF-TZ, co-electrospun from a single nozzle, showed the highest FRET efficiency at 59 %. The blended-electrospinning nanofibers (NF-GH) exhibited exceptional catalytic activity, achieving nearly 90 % conversion of N-phenyltetrahydroisoquinoline (N-phenyl-THIQ) within 4 hours under the visible light (1 W), and the reaction rate constant is 1.8-fold faster than that for fiber with PHB alone. For NF-GH, the conversion of N-phenyl-THIQ retained to 85 % with reused 7 times, demonstrating robust recyclability and photostability. This finding provides new insight into the construction of recyclable FRET-enhanced photocatalysts with broad spectral response and high energy conversion efficiency for organic photochemical reactions.
研究领域:
材料科学
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有机化学
·
光催化
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DOI
高影响因子
Nanoscale Advances
·
2023
·Vol. 5
·期 11
·2986-2993
·
IF 4.7
Peng Shen,Yuqian Liu,Xiaojun Qu,Mingsong Zhu,Ting Huang,Qingjiang Sun
Due to their appealing properties, nanomaterials have become ideal candidates for the implementation of computing systems. Herein, an optical keypad lock based on a Förster resonance energy transfer (FRET) nanodevice is developed. The nanodevice is composed of a green-emission quantum dot with a thick silica shell (gQD@SiO2) and peripheric blue-emission quantum dots with ultrathin silica spacer (bQD@SiO2), on which 5,10,15,20-tetrakis(4-sulfophenyl)porphyrin (TSPP) is covalently linked. The nanodevice outputs dual emission-based ratiometric fluorescence, depending on the FRET efficiency of bQD–porphyrin pairs, which is highly sensitive to the metalation of TSPP: values are 59.7%, 44.8%, and 10.1% for bQD–Zn(II)TSPP, bQD–TSPP, and bQD–Fe(III)TSPP pairs, respectively. As such, by using the competitive chelation-induced transmetalation of TSPP, the nanodevice is capable of implementing a 3-input keypad lock that is unlocked only by the correct input order of Zn(II) chelator, iron ions, and UV light. Interestingly, the reversible transmetalation of TSPP permits the reset (lock) operation of the keypad lock with the correct input order of ascorbic acid, Zn(II), and UV light. Application of the nanodevice is exemplified by the construction of paper and cellular keypad locks, respectively, both of which feature signal readability and/or high resettability, showing high potential for personal information identification and bio-encryption applications.
研究领域:
材料科学
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生物医学工程
·
化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2023
·
IF 4.7
Ting Zhou,Yu Chen,Teng Luo,Jun Song,Junle Qu
In situ monitoring multidrug release in complex cellular microenvironments is significant, and currently, it is still a great challenge. In this work, a smart nanocarrier with the capability of codelivery of small molecules and gene materials as well as with Förster resonance energy transfer (FRET)-modulated fluorescence lifetime is fabricated by integrating gold nanoparticles (the acceptor) into dual-mesoporous silica loaded with multiple drugs (the donor). Once internalized into tumor cells, in weakly acidic environments, the conformation switch of the polymer grafted on nanocarriers causes its shedding from the mesopores, triggering the release of drugs. Simultaneously, based on the strong overlap between the emission spectrum of donors and the absorption spectrum of the acceptors, any slight fluctuation of the dissociation of the drugs from nanocarriers can result in a change in the FRET-modulated lifetime signal due to the extraordinarily sensitive FRET signal to the separation distance between donors and acceptors. All these implied the potential applications of this nanoplatform in various biomedical fields that require the codelivery and real-time monitoring of multidrug-based synergistic therapy.
研究领域:
生物医学工程
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纳米医学
·
药理学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2024
·Vol. 149
·期 24
·5784-5790
·
IF 3.6
Yi-Xuan Li,Yu-Xuan Dai,Ju-Zheng Wang,Jérome Chauvin,Xue-Ji Zhang,Serge Cosnier,Robert S. Marks,Dan Shan
The concentration of zinc ions in semen is significantly correlated with sperm viability and male fertility. In this work, a reliable ratiometric fluorescence probe (ZIF-9-TCPP) based on the efficient Förster resonance energy transfer (FRET) process between two luminophores, benzimidazole (BIM) and meso-tetra (4-carboxyphenyl) porphyrin (TCPP) for Zn2+ detection has been constructed, where the emissions of BIM and TCPP are used as reference and detection signals. The proximity of BIM and TCPP in one framework (ZIF-9-TCPP) and the overlapped spectra between BIM and TCPP afford the attainment of a highly efficient FRET (around 90% efficiency). Efficient FRET improves the fluorescence intensity of porphyrin to enhance the sensitivity of detection. The unique spectral shift resulting from Zn2+ binding to the porphyrin ring ensures the selectivity of detection. In addition, the response mechanism of the proposed ratiometric probes to Zn2+ has been investigated. This work provides a convenient way to design an efficient FRET system and a promising method for sperm mobility detection.
研究领域:
生物化学
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生殖医学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2022
·Vol. 32
·期 5
·1815-1823
·
IF 2.525
Yang Jing,Huang Yan,Cui Hanyue,Li Li,Ding Yaping
In this work, the fluorescent sensor based on fluorescence resonance energy transfer (FRET) and electrostatic interaction (EI) was prepared for the ratiometric and visual detecting S 2– . The FRET fluorescent sensor consists of two fluorophores, with carbon dots (CDs) as energy donors and silver nanoclusters (Ag NCs) as acceptors. At 390 nm excitation, CDs and Ag NCs showed two well-separated peaks at 445 nm and 660 nm, separately. The existence of S 2– caused the red fluorescence at 660 nm to be quenched, whereas the blue fluorescence at 445 nm was restored, and the fluorescence color of the ratiometric sensor changed from pink to blue. It could be employed in ratiometric and visual detecting S 2– . The linear range of quantitative detection S 2– was 0.5–100 μM, and its detection limit was 0.35 μM. CDs-Ag NCs could be used for detecting S 2– in mineral water and tap water. The results showed that the FRET ratiometric fluorescent sensor exhibits good anti-interference and high selectivity for detecting S 2– in environmental water samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Chemical Physics Impact
·
2023
·
IF 2.2
Yao Chen,Hao Li,Dening Peng,Hexing Li,Yinghong Yue,Zhenfeng Bian
Humanity's need for clean air has stimulated their research interest in the remediation of volatile organic compounds (VOCs). As an environmentally friendly pollution control technology, photocatalysis can easily generate oxidative free radicals for the mineralization of VOCs. Herein, we report a Förster resonance energy transfer (FRET)-inspired Ag/SrSO 4 /TiO 2 photocatalyst with 2.5-fold higher activity for toluene degradation than pristine TiO 2 . The study of the catalyst reaction mechanism shows that the interlayer SrSO 4 between the plasmonic Ag and the photocatalyst TiO 2 is the key factor to guide the unidirectional transfer of electrons . A proper thickness of the insulator interlayer (whether SrSO 4 or BaSO 4 ) can reduce the recombination efficiency of photo-generated electrons and holes, resulting in an excellent toluene degradation ability. This finding demonstrates the excellent versatility of the catalyst system, which provides a novel direction for the modification of typical photocatalysts.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
MACROMOLECULAR RAPID COMMUNICATIONS
·
2023
·Vol. 44
·期 13
·2300097
·
IF 4.6
Yayuan Liu,Fuyue Tian,Jing Hu,Nanying Ning,Bing Yu,Ming Tian
Intelligent fluorescent coatings have received widespread attention in the encryption technique field such as anti-counterfeit, while the traditional fluorescent coatings are easy to be damaged by external force. In this study, the fluorescent and self-healing anti-counterfeit coatings are prepared after rational molecular design. Two polymers containing fluorescent groups, including polyurethane containing the anthracene (AN) side group and polyimide containing the naphthalenediimide (NDI) structure are synthesized to realize multiple color changes with fluorescence through simply blending. Due to the overlapping fluorescence spectra of the groups, enabling the fluorescence resonance energy transfer effect (FRET), the coating with different group ratios exhibits tunable fluorescence under the same excitation light, providing diverse patterns. Moreover, due to the reversible photo-crosslinking and thermal de-dimerization properties of anthracene, the recording, erasure, and rewriting of the surface pattern can be realized, and the fluorescent anti-counterfeit coatings exhibit excellent self-healing properties after cross-linking due to the FRET effect, which solves the problem of poor healing and ensures the stability and integrity. The fluorescent coating with cross-linked structure and self-healing behavior based on the FRET effect greatly expands the functional applications of traditional polyurethane materials.
研究领域:
材料科学
·
光学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）