RSC Advances
·
2024
·Vol. 14
·期 35
·25561-25570
·
IF 3.9
Chen Chen,Mei Zhao,Jingyi Guo,Xia Kuang,Zilin Chen,Fang Wang
N6-Methyladenosine (m6A) is a prevalent modification in eukaryotic mRNAs and is linked to various human cancers. The fat mass and obesity-associated protein (FTO), a key m6A demethylase, is crucial in m6A regulation, affecting many biological processes and diseases. Detecting FTO is vital for clinical and research applications. Our study leverages the specific cleavage properties of the MazF endoribonuclease to design an electrochemical method with signal amplification guided by streptavidin-horseradish peroxidase (SA-HRP), intended for FTO detection. Initially, the compound N3-kethoxal is employed for its reversible tagging ability, selectively attaching to guanine (G) bases. Subsequently, dibenzocyclooctyne polyethylene glycol biotin (DBCO-PEG4-Biotin), is introduced through a reaction with N3-kethoxal. HRP is then employed to catalyze the redox system to enhance the current response further. A promising linear correlation between the peak current and the FTO concentration was observed within the range of 7.90 × 10−8 to 3.50 × 10−7 M, with a detection limit of 5.80 × 10−8 M. Moreover, this method assessed the FTO inhibitor FB23's inhibitory effect, revealing a final IC50 value of 54.73 nM. This result aligns with the IC50 value of 60 nM obtained through alternative methods and is very close to the values reported in the literature. The study provides reference value for research into obesity, diabetes, cancer, and other FTO-related diseases, as well as for the screening of potential therapeutic drugs.
研究领域:
分子生物学
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医学检验
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癌症研究
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产品关联
DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2021
·
IF 3.08
Jiaxiong Xu,Xiao Tian,Lei Qiu
Fluorine doped tin oxide (FTO) has low lattice mismatch with Cu 2 ZnSnS 4 (CZTS) thin film and has been considered as a suitable substrate material for CZTS. The interface properties between CZTS and FTO play an important role in the photovoltaic performance of CZTS thin film solar cells. However, studies on the properties of the CZTS/FTO interface are scarce. In this work, the phase structures, compositions, morphologies, and electrical contact properties of the CZTS/FTO interfaces were investigated. CZTS thin films with annealing temperature from 500 °C to 580 °C were deposited on FTO-coated glass substrate by sol-gel, spin-coating, and annealing processes. The CZTS/FTO interfaces were characterized by X-ray diffraction, Raman spectroscopy , energy-dispersive spectroscopy, secondary ion mass spectroscopy , scanning electron microscopy, resistivity, and current–voltage ( I – V ) characteristic curve measurement. The results show the successful formation of CZTS/FTO interfaces. No interfacial layer was found at all the CZTS/FTO interfaces, indicating the stability of the CZTS/FTO interface during high temperature treatment. The I – V curves of all the CZTS/FTO structures were near linear, revealing the formation of ohmic contact between CZTS and FTO. The experimental results in this work indicate the feasibility of FTO as a substrate and back electrode for CZTS thin films.
研究领域:
材料科学
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光伏技术
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薄膜太阳能电池
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DOI
高影响因子
iScience
·
2023
·
IF 5.8
Shuai Chen,Peng Song,Yu Wang,Zeng Wang,Jiaming Xue,Yicheng Jiang,Yan Zhou,Jie Zhao,Liming Tang
Circular RNA (circRNA) is a special category of non-coding RNA that has garnered increasing attention in the exploration of lipid metabolism. However, the functional regulation mechanisms of circRNAs in obesity diseases remain unclear. By whole transcriptome sequencing, a total of 164 circular RNAs were found to exhibit differential expression between lean and obese individuals. RT-qPCR was used to detect significant expression of circMAPK9 in obese individuals, and it was closely related to BMI. Western blot, triglyceride detection, and Oil Red O staining were employed to investigate the role of circMAPK9/hsa-miR-1322/FTO in adipogenesis. In adipocytes, the connection between hsa-miR-1322 and circMAPK9 was verified using fluorescence in situ hybridization, luciferase reporter assay, and RNA immunoprecipitation. It was found that circMAPK9 competed for binding hsa-miR-1322 in the cytoplasm, weakening the inhibitory effect on FTO and promoting adipogenesis. Our study revealed the regulatory mechanism and important role of circMAPK9 in the process of adipogenesis.
研究领域:
分子生物学
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基因组学
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代谢
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DOI
PubMed
高影响因子
APPLIED PHYSICS LETTERS
·
2019
·Vol. 115
·期 5
·
IF 3.521
Wang Jinxin,Jiang Guicheng,Huang Weicheng,Liu Danqing,Yang Bin,Cao Wenwu
Materials with an electrocaloric effect (ECE) have been extensively studied for their cooling applications. Most ECE films are oriented and are difficult to integrate into industrial production processes. Here, an ECE ( ΔS = 4.48 J K −1 kg −1 and ΔT = 6.4 °C at ΔE = 390 kV cm −1 ) is obtained in an unoriented PbZr 0.95 Ti 0.05 O 3 (PZT) film fabricated on an FTO substrate using an improved sol-gel method. To improve the ECE, Si-doped PZT films with 1–4 mol. % of Si are deposited. X-ray diffraction and X-ray photoelectron spectroscopy results confirm that Si was doped into the PZT lattice. The orthorhombic and tetragonal antiferroelectric phases coexist in the PZT/0.01 Si film. A large ECE ( ΔS = 5.89 J K −1 kg −1 and ΔT = 8.5 °C at ΔE = 390 kV cm −1 ) is acquired in the PZT/0.01 Si film, which was measured by an indirect method. This work demonstrates that ECE of unoriented PZT antiferroelectric films on FTO substrates can be improved by a certain amount of Si doping.
研究领域:
材料科学
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物理学
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DOI
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Xinyao Zheng,Shaohui Deng,Yuan Li,Zhipeng Luo,Ziqi Gan,Zhaoping Zheng,Rui Xu,Shan Xiao,Yuxiong Cai,Jianfu Meng,Li Li,Changxing Li,Xiaowen Xue,Wei Dai,Si Qin,Mengying Wang,Kang Zeng,Zecong Xiao,Laixin Xia
Chronic diabetic wounds are a prevalent and severe complication of diabetes, contributing to higher rates of limb amputations and mortality. N6-methyladenosine (m 6 A) is a common RNA modification that has been shown to regulate tissue repair and regeneration. However, whether targeting m 6 A could effectively improve chronic diabetic wound healing remains largely unknown. Here, we found a significant reduction in mRNA m 6 A methylation levels within human diabetic foot ulcers, and the expression level of fat mass and obesity-associated protein (FTO) was significantly increased. We identified that m 6 A modifies the RNA of matrix Metalloproteinase 9 (MMP9), a key factor in diabetic wound healing, to regulate its expression. Importantly, we developed a ROS-scavenging nanocolloidal hydrogel loaded with an FTO inhibitor to increase the m 6 A level of MMP9 RNA in wounds. The hydrogel can effectively accelerate wound healing and skin appendage regeneration in streptozotocin-induced type I diabetic rats at day 14 (approximately 98% compared to 76.98% in the control group) and type II diabetic db/db mice at day 20 (approximately 93% compared to 60% in the control group). Overall, our findings indicate that targeting m 6 A with ROS-scavenging hydrogel loaded with FTO inhibitor may be an effective therapeutic strategy for diabetic wound healing.
研究领域:
分子生物学
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再生医学
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药剂学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PHYSICS LETTERS
·
2021
·
IF 2.328
Qianqian Shang,Xin Tan,Mingcan Chen,Songyue Han,Tao Yu
High-efficient SERS substrates containing dense hot spots were fabricated by assembling 3D Ag nanoflowers (AgNFs)@Au-FTO substrate by using citrate ions as the shape-guiding agent. The vertical growth of 3D AgNFs could be attributed to the synergistic effects of the concentration gradient layer of Ag + and the density gradient of the charge carrier. Combined with the simulation result of FDTD method, the enhanced SERS sensitivity and repeatability of AgNFs@Au-FTO could be attributed to the enlarged specific surface area, and the improved density of hot spots contributed by the overlap between the vertically stacked AgNSs with a high population of sharp edges.
研究领域:
材料科学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
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2017
·Vol. 27
·期 4
·1467-1477
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IF 1.461
Ren Ting,Wang Zechun,Zhang Lijiao,Wang Ning,Han Xinxin,Wang Ruiyong,Chang Junbiao
In this work, the interaction between camptothecin (CPT) analogs and fat mass and obesity associated (FTO) was investigated using spectroscopy and molecular docking. From the experimental results, it was found that the CPT analogs caused the fluorescence quenching of FTO through a static quenching procedure. The binding constants and thermodynamic parameters at three different temperatures, the number of binding sites were obtained, which suggested that the hydrophobic interaction and electrostatic force played major role in the reaction between CPT analogs and FTO. Results revealed that 10-hydroxycamptothecin was the strongest quencher.
研究领域:
生物化学
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药物化学
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分子生物学
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DOI
PubMed
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2024
·
IF 5.3
Lingli Jiang,Rui Liang,Qing Luo,Zhe Chen,Guanbin Song
Fat mass and obesity-associated protein (FTO) is an N6-methyladenosine (m6A) demethylase and plays critical oncogenic roles in multiple cancers. Here we show that FTO is an effective target in hepatocellular carcinoma (HCC). FTO is highly expressed in patients with HCC. Genetic depletion of Fto dramatically attenuated HCC progression in mice. Pharmacological inhibition of FTO by FB23/FB23-2 markedly suppressed the proliferation and migration of HCC cell lines in vitro and inhibited HCC tumorigenicity in xeno-transplanted mice. Mechanistically, FB23-2 suppressed the expression of Erb-b2 receptor tyrosine kinase 3 (ERBB3) and human tubulin beta class Iva (TUBB4A) by increasing the m6A level in these mRNA transcripts. The decrease in ERBB3 expression resulted in the inhibition of Akt-mTOR signaling, which subsequently impaired the proliferation and survival of HCC cells. Moreover, FB23-2 disturbed the stability of the tubulin cytoskeleton, whereas overexpression of TUBB4A rescued the migration of HCC cells. Collectively, our study demonstrates that FTO plays a critical role in HCC by maintaining the proliferation and migration of cells and highlights the potential of FTO inhibitors for targeting HCC.
研究领域:
癌症生物学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2017
·
IF 3.133
Jinxin Wang,Guicheng Jiang,Weicheng Huang,Danqing Liu,Bin Yang,Wenwu Cao
PbTiO 3 (PTO) and Cu-doped PbTiO 3 thin films were deposited on the fluorine doped tin oxide (FTO) by a sol-gel route. Detailed investigations were made on the effects of Cu doping on the crystal structure and ferroelectric properties of the thin films. With increasing Cu amount, the crystal structure of Cu-doped films gradually transits from tetragonal to pseudo-cubic phase. Worth mentioning, the Cu-doped can induce a superior giant remnant polarization of ∼55.7 μC/cm 2 and a relatively low coercive field of 304 kV/cm when the molar concentration of Cu in the sol is 0.02. The experimental results show that Cu-doped PbTiO 3 is very a promising candidate for ferroelectric random access memory (FERAMs) applications.
研究领域:
材料科学
·
物理学
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DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 47
·41287-41293
·
IF 3.289
Beili Pang,Lifeng Dong,Shuai Ma,Hongzhou Dong,Liyan Yu
The attractiveness of graphene arises from its low cost, transparency, high electrical conductivity, chemical robustness, and flexibility, as opposed to the rising cost and brittleness of FTO. In particular, graphene is emerging as a possible substitute for FTO in flexible displays, touch screens, and solar cells. The main goal of our work is to develop new conductive oxide free graphene-based counter electrodes for dye sensitized solar cells (DSSCs). Graphene nanoplates are modified by silane coupling agent to introduce vinyl groups, and then mixed with polyurethane adhesive and cast on glass substrate. The film is irradiated by UV source and heat treated under Ar/H2. A network graphene film is formed and tightly bonded on glass substrate with enhanced electrical conductivity. The structure of network graphene is investigated by XPS, TGA and SEM. The DSSCs with network graphene counter electrode exhibit power conversion efficiencies of 9.33%, much better than those with FTO electrodes (4.05%).
研究领域:
材料科学
·
能源技术
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产品关联
DOI
高影响因子
Nano Research
·
2023
·Vol. 16
·期 8
·11042-11047
·
IF 9.9
Wang Lei,Zhang Yuehan,Sun Xiaoxuan,Li Yunhui,Zhai Junfeng,Dong Shaojun
Eco-friendly chemical oxygen demand (COD) sensors are highly desired with respect to the importance of COD determination in environmental protection. In this work, a new FTO/TiO 2 /PbO 2 (FTO = fluorine-doped tin oxide) electrode was fabricated with a two-step method and used as an eco-friendly electrochemical COD sensor. The interlayer TiO 2 was employed to strengthen the adhesion of PbO 2 on the FTO substrates by providing a large TiO 2 /PbO 2 interface area. The effects of the factors including applied potential, supporting electrolyte concentration and stirring speed on the sensing performance were investigated. Under the optimized conditions, linear responses to the COD of water with different COD sources were achieved, and a linear range from 5 to 120 mg/L was obtained in the case of sucrose as the COD source. The relative standard deviations (RSD) were determined to be less than 9% for the glucose solutions with the COD of 7.5, 12.5 and 17.5 mg/L. For real sample analysis, the obtained results were comparable with those measured with the conventional dichromate method, with a relative error less than 11%.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
iScience
·
2024
·
IF 4.6
Lele Xu,Yuting Chen,Tao Wu,Jiaqi Fan,Yuying Hu,Xuefeng Gao,Yuliang Wang,Tao Chen,Xueting Zhao,Min Zeng,Fei Wang,Qingyou Zheng,Xiaojuan Pei,Dinglan Wu
Polyadenosine diphosphate-ribose polymerase inhibitors (PARPi) represent a promising novel treatment for castration-resistant prostate cancer (CRPC) with encouraging results. However, the combination targets in CRPC remain largely unexplored. N6-methyladenosine (m 6 A) has been shown to play a crucial role in cancer progression and DNA damage response. Here, we observed a higher overall level of m 6 A and a downregulation of Fat mass and obesity-associated protein (FTO), which correlated with unfavorable clinicopathological parameters in prostate cancer (PCa). Functionally, reduced FTO promotes PCa growth, while overexpression of FTO has the opposite effect. Mechanistically, FOXO3a was identified as the downstream target of FTO in PCa. FTO downregulates the expression of FOXO3a in an m 6 A-dependent manner, leading to the degradation of its mRNA. Importantly, DNA damage can degrade FTO through the ubiquitination pathway. Finally, we found that overexpression of FTO can enhance the effect of PARPi on PCa. Therefore, our findings may provide insight into novel therapeutic approaches for CRPC.
研究领域:
癌症生物学
·
分子生物学
·
表观遗传学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）