Renchi Chen,Yingyin Wu,Xiaobo Wei,Zhihai Huang,Linchun Mao
Both ethylene and abscisic acid (ABA) are identified to be crucial phytohormones that positively regulate strawberry fruit ( Fragaria × ananassa Duch.) ripening. In this work, the interplay between ethylene and ABA involved in the ripening of strawberry fruit was explored. Results suggested that ethylene promoted ABA biosynthesis via repressing miR161 expression. Meanwhile, miR161 silencing (STTM161) verified that miR161 negatively regulated ABA biosynthesis via suppressing FaNCED1 expression. In addition, dual-luciferase reporter demonstrated the suppression of miR161 on the promoter of FaNCED1 . These results illuminated a potential molecular mechanism of the collaboration between ethylene and ABA in postharvest strawberry fruit through miR161.
Jingxin Chen,Linchun Mao,Hongbo Mi,Wenjing Lu,Tiejin Ying,Zisheng Luo
Fruits are prone to suffer water-deficit stress and accelerated senescence after harvest. To understand the effect of postharvest water-deficit on the senescence progress of strawberry fruit, fruit peduncles were dipped into water or abscisic acid solution and stored at 20 °C with 50% relative humidity in the dark. The results showed that detached fruit without water supply through the peduncles suffered serious water-deficit stress with great weight loss and low moisture content. However, fruit absorbing water through their peduncles successfully avoided the water-deficit stress with a slight change of weight loss and moisture content. Water-deficit significantly promoted anthocyanin levels with increased PAL, C4H and DFR activities. The expressions of transcriptional factors that regulate anthocyanin biosynthesis ( FaMYB1 , FabHLH3 and FaTTG1 ) were also up-regulated under water-deficit stress. Meanwhile, stress elevated the abscisic acid level as well as FaNCED1 and FaASR expression in strawberry fruit. Furthermore, exogenous ABA application exhibited the similar features with water-deficit stress. These results suggested that postharvest water-deficit would accelerate anthocyanin accumulation in strawberry fruit and that abscisic acid may play a role in the response to water-deficit stress.
危险品化学品经营许可证（带存储）