JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2016
·
IF 2.687
Ying-Di Luo,Qi-Lei Zhang,Xiao-Ming Yuan,Wei Shi,Shan-Jing Yao,Dong-Qiang Lin
Adsorption selectivity is critical important for mixed-mode chromatography with specially-designed ligands. Human immunoglobulin G (hIgG), Fc and Fab fragments were used in the present work to evaluate adsorption behavior and binding selectivity of four mixed-mode resins with the ligands of 4-mercatoethyl-pyridine (MEP), 2-mercapto-1-methylimidazole (MMI), 5-aminobenzimidazole (ABI) and tryptophan-5-aminobenzimidazole (W-ABI), respectively. The resins showed an obvious pH-dependent adsorption behavior. High adsorption capacities were found at neutral pH for hIgG, Fc and Fab, and almost no adsorption happened under acidic conditions. An adsorption selectivity index was proposed to evaluate separation efficiency. High specificity of hIgG/Fc was found at pH 8.9 for MEP resin, and for W-ABI resin at pH 8.0 and 8.9. In addition, isothermal titration calorimetry was used to evaluate ligand-protein interactions. Finally, the separation of hIgG and Fc (1:1) was optimized with mixed-mode resins, and the best separation performance was obtained with W-ABI-based resin. Loading at pH 8.0 resulted in the flow through of Fc with purity of 90.4% and recovery of 98.8%, while elution at pH 3.6 provided hIgG with purity of 99.7% and recovery of 86.5%.
研究领域:
生物化学
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分离科学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2015
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IF 2.729
Zhui Tu,Yang Xu,Jinheng Fu,Zhibing Huang,Yao Wang,Bin Liu,Yong Tao
This work aimed to evaluate novel affinity resin used to purify immunoglobulin G (IgG) with a variable domain of the heavy chain of the heavy-chain antibody (VHH) as an affinity ligand. The VHH, isolated from a naïve camelid single-domain phage display library, exhibits not only affinity to the fragment crystallizable (Fc) region of IgG but also high thermal stability . This anti-Fc VHH (AFV) was expressed as a soluble protein in Escherichia coli and purified using a simple heat treatment procedure. The effects of pH and NaCl concentrations on the capacity of AFV resin were also investigated. Results showed a robust property of the AFV resin. It could bind IgGs at various pH conditions (from 6.0 to 9.0) and NaCl concentrations. The static binding capacities of AFV resin ranged from 3.40 ± 0.53 mg/ml to 15.04 ± 0.37 mg/ml measured using rabbit, mouse, and human IgGs. The bound IgGs can be efficiently eluted at pH 5.0, which is conducive to acid-sensitive IgGs and prevents the aggregation of IgGs. After 10 purification cycles or a 7-day period of storage at 37 °C, recovery did not decrease. These findings suggested that VHHs from non-immunized library could also be robust and functional reagent as an affinity purification ligand.
研究领域:
生物技术
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生物化学
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分子生物学
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蛋白质工程
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Xiaoyi Lv,Xiaoyun Xu,Tian Miao,Xufeng Zang,Chao Geng,Yanping Li,Bo Cui,Yishan Fang
Fluorescent dyes have been considered as one of the most potential candidates for electrochemiluminescence (ECL) reagent due to the excellent photophysical and electrochemical characteristics in recent years. However, the severe self-absorption effect caused by narrow stokes shift inevitably limits its application for ECL. In this work, a new type of ECL emitter, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene derivative, a BF2 complex of hydrazine-schiff base linked bispyrrole, namely BPYHBF, was prepared and employed as a probe to label the competitor of target in the immunosensor, which could emit powerful ECL signal with tri- n -propylamine. The structure and ECL property of BPYHBF were investigated. Additionally, multi-walled carbon nanotube/ferrocenecarboxaldehyde-metal organic framework (MWCNTs/Fc-MOF) as sensing substrate for Ab1 incubation was synthesized, where the large specific surface area and stable internal reference electrochemical signals improved the sensitivity and reliability of the sensor by self-correction. Herein, a novel ratiometric competitive immunosensor based on the ECLBPYHBF/DPVFc was prepared for ultrasensitive detection of aflatoxin B1 (AFB1). The ratio value is linear in the range from 10 fg/mL to 100 ng/mL, and the detection limit is 5.39 fg/mL. The prepared sensor exhibited excellent sensitivity, accuracy and reproducibility, and achieved satisfactory detection towards AFB1 in actual samples, this demonstrates a promising application in biomolecules detection.
研究领域:
分析化学
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食品安全
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生物传感
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Jiale Lu,Amin Jv,Hongyu Zhao,Guangbo Che,Bo Feng,Yufei Zhang,Chunbo Liu,Renquan Guan
MIL-101(Cr) is an ideal material for wastewater treatment due to its large specific surface area, excellent water stability, and chemical stability. However, its limited light absorption capacity hinders its application. We loaded ferrocene carboxylic acid (Fc-COOH) onto MIL-101(Cr) by a post-synthetic modification method, which resulted in the MOF possessing excellent optical properties while maintaining its original skeleton structure. The MIL-101(Cr)/Fc-COOH (1:2), (abbreviated as CFCC-2) exhibited the best performance, achieving a removal rate of 96% for tetracycline (TC) in just 30 min. This removal rate is significantly higher compared to MIL-101(Cr) and Fc-COOH, which achieved removal rates of only 15% and 21%, respectively. We propose a possible Z-type heterojunction mechanism between MIL-101(Cr) and Fc-COOH to explain the excellent photocatalytic performance of CFCC-2. The intermediates in TC were identified using LC-MS, and the toxicity of these intermediates was predicted and evaluated through the Toxicity Estimation Software Tool (T.E.S.T.). This study provides new insights into the design and fabrication of MIL-101(Cr)-based catalysts as photodegrading organic pollutants.
研究领域:
环境科学
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材料科学
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化学
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产品关联
DOI
高影响因子
TALANTA
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2024
·
IF 5.6
Feifan Qi,Mengdie Wu,Simin Liu,Wendi Mu,Chunyan Wu,Xinshui Ren,Chuang Rui,Fangfang Wu,Dong Chang,Hongzhi Pan
Carbohydrate antigen 199 (CA199) is the most sensitive marker reported for pancreatic cancer, and it is a difficult task to develop a highly sensitive assay for CA199. During the experiment, a ratiometric electrochemical immunosensor for quantitative analysis of CA199 was prepared for NiCo@Fc-MWCNTs-LDH as the electrode sensing surface and 3D-rGOF@Ag/Au as the label of Ab 2 . NiCo@Fc-MWCNTs-LDH not only provide the required signal for the immunosensor, but also have a layered structure to obtain a large specific surface area, which can provide more sites for the placement of biological molecules. rGOF has the advantages of large specific surface area and high porosity, which can adsorb Ag electrochemical probe through redox reaction. The modification of gold nanoparticles can not only enhance the electrical conductivity of nano-composites, but also immobilize more biomolecules to improve the sensitivity of electrochemical sensors. With the beefing up of CA199 concentration, the oxidation peak current of Ag increases and the oxidation peak current of Fc-COOH decreases. The ratio (y = I Ag /I Fc-COOH ) of two different signals was linear with the logarithm of CA199 concentration in a certain value range. Under optimal conditions, the immunosensor showed excellent performance in the concentration range of 0.0001 U/mL to 10 U/mL, and the detection limit was 5.55 × 10 −4 U/mL. The strategy could clearly discriminate between matched and mismatched targets, demonstrating high specifificity. This approach further detects CA199 in human plasma to differentiate pancreatic cancer patients from healthy individuals with high accuracy. This method also provided a new idea for the ultrasensitive quantitative detection of other biomarkers.
研究领域:
生物医学工程
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肿瘤标志物检测
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纳米材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Jin Li,Xintong Li,Yulian Wang,Qianxu Zhou,Guoqing Shi
The method for immobilizing antibodies on nanoparticles significantly impacts the analytical performance of lateral flow immunoassay (LFIA). As both Fc-binding peptide (FcBP) and Staphylococcal Protein A (SPA) enable the oriented immobilization of antibodies in a similar manner, do LFIA methods based on these two modes exhibit differences in analytical performance? To address this question, AFB1 antibodies were immobilized on Europium nanoparticles (EuNPs) using non-oriented immobilization, oriented immobilization through SPA and Fc-binding peptide FYEILHGAC, generating the immunoprobes Ab@EuNP, Ab-SPA@EuNP, and Ab-FcBP@EuNP, respectively. The corresponding LFIA methods were developed and the performance of the three methods was compared. The results show that the Ab-FcBP@EuNP method immobilizes more “effective antibodies” compared to Ab-SPA@EuNP and Ab@EuNP. When an equivalent amount of “effective antibodies” is immobilized on EuNP, the IC50 value of the LFIA method based on Ab-FcBP@EuNP is significantly lower (2.58 and 3.61 times) than that of the LFIA methods based on Ab-SPA@EuNP and Ab@EuNP, respectively. The LFIA method utilizing Ab-FcBP@EuNP displays detection biases for AFB1 spiked in rice and maize matrices ranging from −8.53% to 3.90%, and biases in the detection results for certified reference materials (CRMs) of rice and maize containing AFB1 range from −11.06% to 6.33%. These results suggest that the LFIA method employing FcBP-based antibody immobilization exhibits superior sensitivity, accuracy, and interference resistance compared to the SPA-based method. The outcomes of this study validate that EuNPs functionalized with FcBP streamline antibody immobilization, minimize antibody usage, and improve the analytical performance of the LFIA, thus demonstrating favorable potential for practical implementation.
研究领域:
食品安全检测
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免疫学
·
分析化学
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产品关联
DOI
高影响因子
Biomaterials Science
·
2023
·Vol. 11
·期 10
·3589-3602
·
IF 6.6
Bo Huang,Wei Guan,Chaofeng Wang,Shuilin Wu,Zhenduo Cui,Yufeng Zheng,Zhaoyang Li,Shengli Zhu,Hui Jiang,Paul K. Chu,Xiangmei Liu
Charge transfer and separation play a critical role in the photocatalytic efficiency of photoresponsive materials and their subsequent applications. Herein, for the first time, we constructed a homo-heterostructured S–Cu–FC/CuS modified carboxymethyl cellulose (CMC) hydrogel doped with graphene oxide (GO) (S–Cu–FC/CuS/GO@CMC) through an in situ ionic complexation reaction, which exhibited enhanced photocatalytic performance under 808 nm near-infrared (NIR) light irradiation. On the one hand, photo-excited charges can rapidly transfer across the homo-heterojunction interface with copper atoms in S–Cu–FC/CuS as both donors and acceptors. On the other hand, the interface between GO and S–Cu–FC/CuS also favors the fast transfer of photogenerated charges. Hence, with the assistance of a photothermal effect, the enhanced photocatalytic performance of S–Cu–FC/CuS/GO endowed the hydrogel with robust sterilization ability for killing 99.5% S. aureus and 100.0% E. coli under 10 min NIR light irradiation. An in vivo test disclosed that this hydrogel could also accelerate the healing of bacteria-infected wounds.
研究领域:
材料科学
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光催化
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2020
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IF 8.758
Zheng Deng,Chao Fang,Xu Ma,Xiang Li,Yu-Jia Zeng,Xinsheng Peng
Metal–organic frameworks (MOFs) have been identified as promising materials for the delivery of therapeutics to cure cancer owing to their intrinsic porous structure. However, in a majority of cases, MOFs act as only a delivery cargo for anticancer drugs while little attention has been focused on the utilization of their intriguing physical and chemical properties for potential anticancer purposes. Herein for the first time, an ultrathin (16.4 nm thick) ferrocene-based MOF (Zr-Fc MOF) nanosheet has been synthesized for synergistic photothermal therapy (PTT) and Fenton reaction-based chemodynamic (CDT) therapy to cure cancer without additional drugs. The Zr-Fc MOF nanosheet acts not only as an excellent photothermal agent with a prominent photothermal conversion efficiency of 53% at 808 nm but also as an efficient Fenton catalyst to promote the conversion of H2O2 into hydroxyl radical (•OH). As a consequence, an excellent therapeutic performance has been achieved in vitro as well as in vivo through this combinational effect. This work aims to construct an “all-in-one” MOF nanoplatform for PTT and CDT treatments without incorporating any additional therapeutics, which may launch a new era in the investigation of MOF-based synergistic therapy platforms for cancer therapy.
研究领域:
纳米技术
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癌症研究
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材料科学
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药物递送
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Zhiren Zhou,Lina Zou,Ping Zhang,Chunmei Li,Ye Zhu,Huanyu Ren,Zheng Li,Huiru Niu,Hao Liao,Xiaojing Zhang,Shanshan An,Fei Ren,Xiuhong Ge,Lang Cheng,Feiyan Yang,Hongzhi Pan,Shengzhong Rong,Dong Chang,Hongkun Ma
Survivin serves as a crucial biomarker for bladder cancer, so exploring the detection method is essential. Therefore, a sensitive ratiometric electrochemical immunosensor was developed. In brief, a double-layered amino graphene oxide (NH 2 -GO) sandwich enhanced by Fe-Ni@Ti 3 C 2 T x (NH 2 -GO/Fe-Ni@Ti 3 C 2 T x /NH 2 -GO) significantly amplified the detection signal. ferrocene@Au nanoparticles (Fc@AuNPs) provided a reference signal, and Ab 1 was anchored via an Au-N bond. Moreover, AuPt-methylene blue (AuPt-MB) served not only as a secondary signal but also anchored Ab 2 through Au-NH 2 and Pt-NH 2 bonds. Black phosphorus nanoplates (BPNPs) improved conductivity and provided additional binding sites for AuPt-MB. The immunosensor exhibited a broad detection range (0.001~1000 ng/mL) and a low detection limit (0.00065 ng/mL), facilitating successful survivin detection in human urine samples with promising clinical analysis applications.
研究领域:
生物医学工程
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分析化学
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肿瘤学
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产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Baohan Wen,Haoyi Ren,Wenjing Lai,Min Wang,Mingzhe Jiang,Yingying Cheng,Yige Li,Chenglin Hong,Yu Qi
Early cancer diagnosis is crucial due to a significant increase in cancer incidence over the last three decades. A highly sensitive electrochemiluminescence technique was developed for carcinoembryonic antigen (CEA) detection. This method uses Ru(bpy)32+ as the luminescent substance, and the silica and polyacrylamide gel composite as the carrier. Ru(bpy)32+ is loaded into it to prevent its leakage. At the same time, biological molecules such as antibodies or antigens are immobilized, thereby improving the stability and reaction activity of the system. Additionally, Au-Fc-MOF was synthesized to act as a quencher and secondary antibody label, absorbing the emitted energy from the ECL emitter to improve detection accuracy across various CEA concentrations. Potassium persulfate was employed as a co-reactant to generate SO4•- radicals, intensifying the luminescence output of the system. The findings revealed that the developed immunosensor exhibited strong linearity within the concentration range of 10-5 ng/mL to 10 ng/mL, with a remarkably low detection limit of 5.03 fg/mL. This electrochemical luminescence immunosensor, characterized by its simple preparation, cost-effectiveness, and superior performance, demonstrates significant potential in advancing early CEA detection methodologies.
研究领域:
分析化学
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生物传感器
·
癌症诊断
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纳米材料
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电分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yue Du,Chong-Mei Xu,Yu-Min Zhang,Zheng-Xuan Pan,Feng-Shan Wang,Hong-Ming Yang,Jin-Bao Tang
Covalent and oriented immobilization of antibodies (Abs) can substantially improve the sensitivity and stability of solid-phase immunoassays . By modifying the natural Abs with functional groups that provide unique handles for further conjugation, Abs could be immobilized onto the solid matrices with uniform orientation. Herein, an effective approach for Fc-specific modification of Abs was developed for the oriented and covalent immobilization of Abs. Twelve photoreactive Z -domain variants, incorporated with a photoactivable probe ( p -benzoyl- L -phenylalanine, Bpa) at different positions and carrying a C-terminal Cys-tag (i.e. Z Bpa -Cys variants), were individually constructed and produced in Escherichia coli and tested for photo-cross-linking to various IgGs. The different Z Bpa -Cys variants demonstrated large differences in photo-conjugation efficiency for the tested IgGs. The conjugation efficiencies of 17th Z Bpa -Cys ranged from 90 % to nearly 100 % for rabbit IgG and mouse IgG 2a , IgG 2b and IgG 3 . Other variants, including 5th Z Bpa -Cys, 18th Z Bpa -Cys, 32th Z Bpa -Cys, and 35th Z Bpa -Cys, also displayed conjugation efficiencies of 61 %–83 % for mouse IgG 1 , IgG 2a and IgG 3 . Subsequently, the photo-modified Abs, namely IgG–Cys conjugates, were covalently immobilized onto a maleimide group-functionalized solid-phase carrier on the basis of the reaction of sulfhydryl and maleimide . Thus, a generic platform for the controlled and oriented immobilization of Abs was developed, and the efficacy and potential of the proposed approach for sensitive immunoassays was demonstrated by detecting human α-fetoprotein.
研究领域:
生物化学
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免疫学
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生物技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
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2021
·
IF 5.373
Jintao Liang,Xiaohang Shi,Huafu Feng,Min Chen,Wenzhan Li,Junxiang Lai,Weipeng Hu,Guiyin Li
In this paper, a novel silicon-based light-addressable potentiometric sensor (LAPS) has been designed for the detection of 1,5-anhydroglucitol (1,5-AG) in human serum. Reduced graphene oxide-chitosan-ferrocene (RGO-CS-Fc)/AuNPs nanohybrids and pyranose oxidase (PROD) enzyme is used to fabricate biological sensitive membrane unit by layer-by-layer assembly technology. When a bias voltage is provided to the LAPS system, the catalytic oxidation reaction between 1,5-AG and PROD to produce H 2 O 2 . The by-product H 2 O 2 can oxidize Fc(Fe 2+ ) ions in RGO-CS-Fc nanohybrids into Fc(Fe 3+ ) ions, which cause the potential of the sensitive membrane surface to change and the potential shift of I-V curve will generate a corresponding offset response. Under the optimal conditions, the potential shift of the LAPS is linearly related to the concentration of 1,5-AG at 10 µg·mL −1 –350 µg·mL −1 with the correlation coefficient of 0.97414. The sensitivity is 0.44273 mV/µg·mL −1 and the lowest detection limit is 10 µg·mL −1 . In addition, the biosensor showed good specificity, acceptable stability and satisfactory recovery rates (91.28%-107.66%), which would be a potential testing methods in actual clinical samples.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
生物传感器
✓ 已验证
DOI
PubMed
高影响因子
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