INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Qi Zhao,Zhencheng Chen,Chen-Wei Shan,Tao Zhan,Chen-Yang Han,Guo-Cheng Han,Xiao-Zhen Feng,Heinz-Bernhard Kraatz
Human chorionic gonadotropin (HCG), a vital protein for pregnancy determination and a marker for trophoblastic diseases , finds application in monitoring early pregnancy and ectopic pregnancy . This study presents an innovative approach employing electrochemical immunosensors for enhanced HCG detection, utilizing Anti-HCG antibodies and gold nanoparticles (AuNPs) in the sensor platform. Two sensor configurations were optimized: BSA/Anti-HCG/c-AuNPs/MEL/e-AuNPs/SPCE with [Fe(CN) 6 ] 3−/4− as a redox probe (1) and BSA/Anti-HCG/PPy/e-AuNPs/SPCE using polypyrrole (PPy) as a redox probe (2). The first sensor offers linear correlation in the 0.10–500.00 pg∙mL −1 HCG range, with a limit of detection (LOD) of 0.06 pg∙mL −1 , sensitivity of 32.25 μA∙pg −1 ∙mL∙cm −2 , RSD <2.47 %, and a recovery rate of 101.03–104.81 %. The second sensor widens the HCG detection range (40.00 fg∙mL −1 -5.00 pg∙mL −1 ) with a LOD of 16.53 fg∙mL −1 , ensuring precision (RSD <1.04 %) and a recovery range of 94.61–106.07 % in serum samples. These electrochemical immunosensors have transformative potential in biomarker detection, offering enhanced sensitivity, selectivity , and stability for advanced healthcare diagnostics.
研究领域:
生物医学工程
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分析化学
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生殖健康
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
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2013
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IF 3.777
Yunlei Zhou,Zhenning Xu,Mo Wang,Xiaomeng Meng,Huanshun Yin
A label-free electrochemical immunosensor for sensitive detection of indole-3-acetic acid (IAA) was developed using gold nanoparticles (AuNPs) functionalized with horseradish peroxidase labeled immunoglobulins G (AuNPs-HRP-IgG) as signal amplification probe and rat monoclonal antibody against IAA as capture probe. AuNPs-HRP-IgG could be immobilized and through the interaction between 4-aminophenylboronic acid and a single N-glycosylation site, which exist in the CH2 domain of IgG. The electrochemical immunosensor was characterized by differential pulse voltammetry (DPV) in 0.1 M PBS (pH 7.4) containing Fe(CN) 6 3−/4− as redox probe. The decreased oxidation peak current of Fe(CN) 6 3−/4− was used to monitor the antibody–antigen interaction. The electrochemical immunosensor exhibited a linear range from 1 × 10 −9 to 5 × 10 −6 M with a limit of detection of 5.5 × 10 −10 M (S/N = 3). The proposed electrochemical immunosensor could well discriminate IAA from other phytohormones, such as gibberellin, abscisic acid and salicylic acid. In addition, IAA extracted from leaf of mung bean sprouts was detected using the developed immunosensor for evaluating its applicability, and the recovery was from 93.5% to 106.75%.
研究领域:
分析化学
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植物生理学
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纳米技术
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生物传感器
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DOI
高影响因子
MICROCHIMICA ACTA
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2020
·Vol. 187
·期 6
·1-10
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IF 6.232
Yang Limin,Gao Yukun,Fang Kai,Sun Hui,Sun Jingtao,Liu Hanyun,Feng Weihua,Jiang Lei
A peptide (Li5–025)-modified gold nanoparticle (AuNP)/(titania (TiO 2 ) + 5,10,15,20-tetrakis(4-aminophenyl)-21H,23H-porphine (TAPP))/glassy carbon electrode (GCE) was developed for lipopolysaccharide (LPS) determination. This electrode not only performs well in the electrochemical impedance determination of LPS in serum but can also be easily regenerated under light irradiation. Using Fe(CN) 6 3−/4- as a redox probe, LPS recognition can be indicated by the significantly increased electron-transfer resistance ( R et ) as a result of the coaction of the increased steric hindrance from the peptide-LPS complex and the electrostatic repulsion between LPS and Fe(CN) 6 3−/4- . The impedimetric signal was acquired in the frequency range 0.1 Hz ~ 100 kHz with an initial voltage of 174 mV and an amplitude of 10 mV. The resistance changes (Δ R et ) are linearly related to the LPS concentrations in a broad range (0.1 pg mL −1 ~ 100 ng mL −1 ) with a low detection limit (0.08 pg mL −1 ). Importantly, the electrode shows high selectivity to LPS from Escherichia coli O55:B5 compared to other bacterial sources and considerable anti-interference to 0.1% fetal calf serum, demonstrating its potential application in clinically relevant samples. Another highlight is that the AuNP/(TiO 2 + TAPP)/GCE surface can be photocatalytically regenerated under light irradiation (50 mW cm −2 , 300–2500 nm) without any obvious damage to the electrode microstructure. After simple peptide re-immobilization, the regenerated electrode demonstrates LPS response similar to the peptide less one, and the deviation is only 2.89% after 5-cycle reuse.
A peptide (Li5–025)-modified AuNP/(TiO 2 + TAPP porphine)/GCE was proposed, which not only has excellent electrochemical analytical performances for LPS assay in serum but also can be reused after light irradiation and subsequent peptide re-immobilization.
研究领域:
分析化学
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生物化学
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纳米技术
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电化学
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生物传感器
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Fa-Ting Xie,Xiao-Lan Zhao,Kuan-Neng Chi,Tong Yang,Rong Hu,Yun-Hui Yang
A ratiometric electrochemical aptasensor is proposed for the detection of thrombin. In the sensor, the iron metal-organic frameworks (Fe MOFs)-labeled aptamer as signal tags was used as signal probe (SP), and the electrolyte solution [Fe(CN) 6 ] 3-/4- was utilized as an inner reference probe (IR). In the presence of thrombin, the signal of Fe-MOFs can be detected. Meanwhile, the signal of [Fe(CN) 6 ] 3-/4 -IR almost remains stable. Accordingly, thrombin concentration can be monitored with the ratio response of I Fe-MOFs-SP /I [Fe(CN)6] 3-/4- -IR . The proposed ratiometric biosensor owns a strong ability to eliminate the disturbance that arises from different DNA loading densities, environmental impact and instrumental efficiency. DNA nanotetrahedron (NTH) with three-dimensional (3D) scaffold can effectively eliminate nonspecific adsorption of DNA and protein. The accessibility of target molecules and loading amounts of signal substances could be increased because of the enhanced mechanical rigidity of well-designed 3D NTH. Thus, detection reproducibility and sensitivity can be further improved. Moreover, the biosensor only requires conjugation with one electroactive substance. The modification procedure can be greatly simplified. The biosensor owns high sensitivity with the detection limit of 59.6 fM. We expect that it will emerge as a generalized ratiometric sensor that may be useful for detecting target analytes .
研究领域:
分析化学
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生物化学
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生物医学工程
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纳米技术
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 7
·1-8
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IF 5.7
Huang Yan,Sun Xuyuan,Yang Jing,Cao Zhiyuan,Wang Rujie,Li Li,Ding Yaping
A molecularly imprinted electrochemical sensor was designed for the selective determination of gatifloxacin (GTX) based on dual functional monomers. Multi-walled carbon nanotube (MWCNT) enhanced the current intensity and zeolitic imidazolate framework 8 (ZIF8) provided a large surface area to produce more imprinted cavities. In the electropolymerization of molecularly imprinted polymer (MIP), p-aminobenzoic acid (p-ABA) and nicotinamide (NA) were used as dual functional monomers, and GTX was the template molecule. Taking [Fe(CN) 6 ] 3−/4− as an electrochemical probe, an oxidation peak on the glassy carbon electrode was located at about 0.16 V (vs. saturated calomel electrode). Due to the diverse interactions among p-ABA, NA, and GTX, the MIP-dual sensor exhibited higher specificity towards GTX than MIP-p-ABA and MIP-NA sensors. The sensor had a wide linear range from 1.00 × 10 −14 to 1.00 × 10 −7 M with a low detection limit of 2.61 × 10 −15 M. Satisfactory recovery between 96.5 and 105% with relative standard deviation from 2.4 to 3.7% in real water samples evidenced the potential of the method in antibiotic contaminant determination. Graphical abstract
研究领域:
分析化学
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电化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
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2016
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IF 4.803
Yonghai Song,Yuan Shen,Jingyi Chen,Yonggui Song,Coucong Gong,Li Wang
A novel pH-dependent immunosensor to detect tumor biomarker, carcinoembryonic antigen (CEA), was developed by using the pH-dependent AuNPs-Ab 2 -glucose oxidase-Concanavalin A as tracing tag and the albumin bovine V-Ab 1 /chitosan-AuNPs/three-dimensional kenaf stem-derived macroporous carbon (3D-KSCs) integrated electrode as signal collector based on a sandwich-type assay mode. The integrated 3D-KSCs electrode provided a large specific surface area to load a large number of Ab 1 effectively and the tracing tag with a lots of pH-dependent surface negative charges repulsed the negatively charged probe of Fe(CN) 6 3− in a pH 9.0 solution. Accordingly, after a sandwich immunoreaction, the quantitative capture of tracing tag on the signal collector greatly hindered the electron transfer of Fe(CN) 6 3−/4− due to the electrostatic repulsion, which improved the sensitivity and selectivity of the immunosensors significantly. It was verified by electrochemical impedance spectroscopy. The resistance to charge transfer of Fe(CN) 6 3−/4− changed proportionally toward CEA concentration from 4 pg mL −1 to 50 ng mL −1 with a low detection limit of 1.3 pg mL −1 ( S / N = 3) and a high sensitivity of 57.57 Ω ng −1 mL. Based on human serum experiments, the sensing platform was proved to be suitable to assay CEA in a practical biosystem. This work not only gives a sensitive way to detect CEA but also provides a potential strategy for immunosensors based on 3D-KSC electrode and pH-dependent behavior.
研究领域:
分析化学
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生物化学
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生物医学工程
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癌症研究
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DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
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2020
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IF 3.784
Xia Zhao,Zanling Huang,Wanying Zhang,Yuting Ke,Fushen Lu,Rongsheng Chen,Kaifu Huo,Liangsheng Hu,Paul K. Chu
Core-shell titanium carbide/carbon (TiC@C) and titanium carbide/nitrogen-doped carbon (TiC@CNx) quasi-aligned nanofiber arrays (NFAs) are fabricated on biomedical Ti-6Al-4V by a simple thermal chemical process in the presence of benzene and pyridine at 800 °C, respectively. The morphology, structure, and composition of the TiC@C and TiC@CNx NFAs are characterized by X-ray diffraction, electron microscopy , energy-dispersive X-ray spectrometry, and Raman scattering spectroscopy. The TiC@C and TiC@CNx nanofibers consisting of highly conducting single-crystalline TiC cores and carbon shells or N-doped carbon shells have a large amount of defects and N doping endows the TiC@CNx NFAs with excellent electrochemical properties . The TiC@CNx NFAs electrode exhibits fast heterogeneous electron transfer (HET) kinetics in the Fe(CN) 6 3−/4− redox couple showing an ideal peak-to-peak potential separation (59 mV) at a high scan rate of 7000 mV s −1 . As a result, the TiC@CNx NFAs electrode has high activity in the simultaneous detection of dopamine (DA), ascorbic acid (AA), uric acid (UA), and serotonin (5-hydroxytryptamine, 5-HT) as well as high sensitivity and selectivity in the determination of DA in the presence of UA, AA, and 5-HT. The linear range for the determination of DA is 0.5–180 μM and detection limit is 8 nM (S/N = 3).
研究领域:
材料科学
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电化学
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纳米技术
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生物医学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）