TALANTA
·
2016
·
IF 4.035
Chao Zhao,Zhongwei Jiang,Ruizhu Mu,Yuanfang Li
In this work, a novel sensor based on fluorescence enhancement of Fe-MIL-88- H 2 O 2 -o-phenylenediamine (OPD) system for the determination of dopamine (DA) was developed. Fe-MIL-88 with intrinsic peroxidase-like catalytic activity tended to oxidize the nonfluorescent OPD into the fluorescent 2,3-diaminophenazine (DAP) with the presence of H 2 O 2 . When DA was introduced, the autoxidation of DA to yield hydroxyl radical (·OH) was further enhanced by Fe 3+ site in Fe 3 -μ 3 -oxo clusters exiting in Fe-MIL-88 MOFs , meanwhile the generated Fe 2+ appeared to involve a Fenton type reaction in the presence of H 2 O 2 to cause a continuous production of ·OH radicals which greatly enhanced the conversion efficiency of the OPD to fluorescent DAP. Thus, the turn-on fluorescence method for the DA detection was established. The linear range was from 50 nm to 30 μM with detection limit 46 nm (3σ/s). Furthermore, the proposed method has been reliably applied to determine dopamine content in dopamine hydrochloride injection and human urine with satisfactory results, which suggests its great potential for assay DA in chemical and biological analytical applications.
研究领域:
分析化学
·
生物医学工程
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高影响因子
ANALYST
·
2015
·Vol. 140
·期 24
·8201-8208
·
IF 4.107
Zheng Juan Sun,Jun Ze Jiang,Yuan Fang Li
Herein, we present a novel strategy based on a “turn-on” fluorescence system made up of metal–organic frameworks Fe-MIL-88 and H2O2 for detecting biothiols in human serum. The nonfluorescent Fe-MIL-88 gives weak fluorescence in the presence of H2O2. Interestingly, it was found that biothiols such as glutathione (GSH), cysteine (Cys) or homocysteine (Hcy) could induce fluorescence turn-on of the Fe-MIL-88/H2O2 system. Under optimal conditions, the relative fluorescence intensity exhibited a good linear relationship in the range from 50 nM–10 μM for GSH (r = 0.994), 50 nM–10 μM for Cys (r = 0.990), and 50 nM–10 μM (r = 0.992) for Hcy; the detection limits of GSH, Cys and Hcy were 30 nM, 40 nM, and 40 nM respectively. Mechanism investigation reveals that biothiols could associate with Fe-MIL-88 via hydrogen bonding and electrostatic interaction followed by redox reaction between biothiols and Fe3+ present in the Fe-MIL-88, Fe3+ was thus reduced to Fe2+, and then Fe2+ could efficiently catalyze the decomposition of H2O2 to yield ˙OH radicals through the Fenton reaction. Besides, biothiols were able to reduce H2O2 to produce ˙OH radicals directly. Thus the Fe-MIL-88 as well as biothiols could cooperatively contribute to the activation of H2O2 to generate higher amounts of ˙OH radicals, which in turn oxidize the free ligand terephthalic acid (BDC) outside or within the Fe-MIL-88 structure to strongly fluorescent hydroxylated terephthalic acid (OHBDC), thereby turning on the fluorescence.
研究领域:
分析化学
·
生物医学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiaomin Fu,Yan Yang,Na Wang,Shihong Chen
Here, the cathodic electrochemiluminescence (ECL) behavior of 3,4,9,10-perylenetetracar-boxylic acid (PTCA) coreacted with hydrogen peroxide was firstly reported. The quenching effect of Fe-MIL-88 MOFs (iron based metal-organic framework) on the ECL of PTCA was found, and the ECL quenching mechanism was investigated, which was speculated to be via the resonance energy transfer from PTCA to Fe-MIL-88 MOFs and steric hindrance effect from Fe-MIL-88 MOFs. Combining with dual molecular recognition strategy and the quenching effect of Fe-MIL-88 MOFs on the ECL of PTCA-H 2 O 2 system, an “on-off” ECL sensor was designed to detect dopamine (DA) with a broad linear range (five orders of magnitude). The detection limit was low to 2.9 × 10 −13 M. The PTCA-H 2 O 2 system would provide a new ECL platform, especially for constructing enzyme based ECL sensor with H 2 O 2 as product in enzymatic reaction. Furthermore, the electrochemiluminescence resonance energy transfer between Fe-MIL-88 metal–organic framework and PTCA would expand a new application of Fe-MIL-88 MOFs in ECL field. The dual molecular recognition strategy ensured the selectivity of the sensor for DA detection.
研究领域:
分析化学
·
材料科学
·
电化学
·
传感器
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Qian Luan,Xin Xiong,Ning Gan,Yuting Cao,Tianhua Li,Dazhen Wu,Youren Dong,Futao Hu
An ultrasensitive colorimetric aptasensor was developed for antibiotics detection, with chloramphenicol (CAP) as model target, using DNAzyme labeled Fe-MIL-88-Pt as novel peroxidase mimic signal tags and target-triggered circular strand-displacement polymerization (CSDP) for signal amplification. The system consists of two components which can partially hybridize with each other: one is capture probe which was formed through immobilizing hairpin DNA containing aptamer sequence on magnetic beads (MB-cDNA), another is signal tag which was constructed through labeling single strand DNAzyme (G-quadruplex/Hemin) which can partially hybrid with cDNA on platinum nanoparticles functionalized Fe-MIL-88 (MIL-88-Pt-DNAzyme). All components of MIL-88, Pt and DNAzyme in the tag can act as peroxidase mimic to triply catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 into a blue-colored oxidized TMB (oxTMB) for the colorimetric readout. Thus distinctive signal can be observed by naked eye even in presence of 0.02 nM tags. In the presence of target and primer, cDNA loop can open to form cDNA/CAP intermediates, enabling primer to hybridize with the exposed sequences of the cDNA, which initiated target assisted CSDP recycles. Then numerous signal tags were released into supernatant to catalyze TMB for color development. There was a liner relationship between the absorbance and the concentration of CAP in the range of 0.1 pM (0.0323 pg/mL) to 1000 pM (323 pg/mL) with the detection limit of 0.03 pM (0.0097 pg/mL). The ultra-high sensitivity was ascribed to the multiplex catalytic activities from the tags and CSDP based signal amplification. Furthermore, this method can produce signals being observed by naked eye to facilitate in-situ detection and be further extended to detect other antibiotics in food just by simply replacing cDNA on the sensing system.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
食品科学
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 28
·22312-22323
·
IF 6.7
Peng Huajie,Gao Di,Xiao Zhengyue,Yue Zhixiang,Xu Peng,Qiu Ping
Compared with natural-enzyme-based detection strategies,
nanozymes
offer notable advantages in stability, environmental tolerance, and
cost-effectiveness. In this study, we designed and synthesized a Pt@Fe-MIL-88
composite with both oxidase (OXD)-like activity and intrinsic fluorescence.
The material efficiently catalyzes 3,3 ′ ,5,5 ′ -tetramethylbenzidine
(TMB) oxidation, generating a color change while quenching its fluorescence.
Glyphosate (Gly) specifically inhibits the OXD-like activity of Pt@Fe-MIL-88,
reducing oxTMB production and thereby alleviating fluorescence quenching,
enabling dual colorimetric and fluorometric signal readout. The inhibition
mechanism was elucidated through FTIR, XRD, XPS characterization,
molecular dynamics simulations, and DFT calculations. Accordingly,
a natural-enzyme-free, colorimetric-fluorometric dual-mode sensing
platform for Gly was established. The platform exhibits high sensitivity,
excellent selectivity, and strong anti-interference capability, with
accuracy and reliability validated by spike recovery tests in real
samples. This work presents a new strategy for developing stable,
rapid, and low-cost multimodal detection methods for pesticide residues.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-8
·
IF 5.479
Cheng Ting,Li Xiang,Huang Peng,Wang Han,Wang Meixia,Yang Wenming
An electrochemical and colorimetric dual-readout method is described for the determination of thrombin. A platinum nanoparticle (Pt NP) modified metal organic framework (MOF) acts as a peroxidase (POx) mimic that causes the formation of a blue product from 3,3′,5,5′-tetramethylbenzidine (TMB) and hydrogen peroxide, with an absorption maximum at 650 nm. In addition, gold nanoparticles enrich initiators that trigger the hybridization chain reaction for dual signal amplification to generate an electrochemical current typically measured at 0.31 V (from −0.5 to −0.1 V) and allow quantitation of thrombin with high sensitivity and over a wide detection range. The colorimetric and electrochemical (dual) thrombin assay produces two kinds of signals which warrants accuracy, diversity, and an option for visual inspection. The dual-channel sensor allows for the quantitative determination of thrombin with a low detection limit (0.33 fM) for the electrochemical method and 0.17 pM for the colorimetric method) and over a wide detection range (1 fM to 10 nM for electrochemical method and 0.5 pM to 1 nM for colorimetric method). The electrochemical detection limit is lower than that of colorimetry, and the linear range is wider, which is more suitable for further quantitative analysis of the target.
Schematic representation of a colorimetric and electrochemical (dual) thrombin assay based on the use of a platinum nanoparticle modified metal-organic framework for color development and hybridization chain reaction for electrochemical signal. C-TBA: complementary sequences of thrombin aptamer, TBA: thrombin aptamer, I-Au NPs: initiators enriched by gold nanoparticles, S-AuE: sensing gold electrode, RS-AuE: reacted sensing gold electrode, TB: thrombin, MB: Methylene Blue, HCR: hybridization chain reaction.
研究领域:
分析化学
·
纳米技术
·
生物传感器
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生物化学
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PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 3
·343-352
·
IF 3.1
Ling-Juan Wang,Wei Han,Ting-Ting Lou,Lin-Lin Ma,Ya-Bing Xiao,Zhou Xu,Mao-Long Chen,Yun-Hui Cheng,Li Ding
For environmental safety, it is important to establish a simple, rapid, and sensitive method for emerging pollutants. Here, a dispersive solid-phase extraction (d-SPE) method based on an iron-based metal–organic framework (Fe-MIL-88-NH2) combined with high-performance liquid chromatography (HPLC) was developed for tetrabromobisphenol A (TBBPA) in water samples. Fe-MIL-88-NH2 was synthesized using a solvothermal method and completely characterized. Fe-MIL-88-NH2 had good water stability and gave a maximum adsorption capacity of 40.97 mg g−1 for TBBPA. The adsorption of TBBPA on Fe-MIL-88-NH2 followed Langmuir adsorption models and a pseudo-second-order kinetic model. The bromine ion and the hydroxyl group of TBBPA could form strong hydrogen bond interactions with the amino protons around the cavity of Fe-MIL-88-NH2, which was in accord with the molecular simulation calculations. Furthermore, several important d-SPE parameters were optimized, such as the amount of materials, extraction time, pH, ionic strength, elution solvent type, and volume. The established method showed good linearity in the concentration range of 0.005–100 μg g−1 (r2 ≥ 0.9996). This method's limits of detection (LOD) and quantification (LOQ) were 0.001 μg g−1 and 0.005 μg g−1, respectively. The recoveries in spiked water samples ranged from 87.5% to 104.9%. The proposed method was applied successfully to detect TBBPA in environmental water samples.
研究领域:
环境科学
·
分析化学
·
材料科学
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PubMed
高影响因子
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