SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Li Hou,Yuemei Qin,Tianran Lin,Ying Sun,Fanggui Ye,Shulin Zhao
The synthesized Fe-MIL-88NH 2 was used as a nanozyme and fluorescence probe to successfully construct a selective fluorescent sensing platform for one step determination of catechol. Fe-MIL-88NH 2 exhibited excellent peroxidase mimic property as well as fluorescence property. With the addition of H 2 O 2 and catechol, Fe-MIL-88NH 2 would catalyze the H 2 O 2 to form hydroxyl radicals ( OH). Then, the oxidation of catechol would be triggered by the formed OH, which resulted in the generation of 1, 2-benzoquinone. Afterwards, Michael reaction between the oxidation products of catechol (1, 2-benzoquinone) and Fe-MIL-88NH 2 ligands was triggered and then quenched the fluorescence of Fe-MIL-88NH 2 . The principle of the selective determination of catechol was due to the assistant effect of Michael reaction. Based on the above sensing mechanism, a fluorescent sensing platform for the determination of catechol was established. The limit of detection was 0.0913 μmol L −1 (3S 0, n=8 /K) within a fast response of 10 min. This simple and selective strategy, based on the Michael reaction and the superiority of Fe-MIL-88NH 2 nanozyme, provided a concise sensing platform for catechol determination, which broadened the scope of application of nanozyme and showed a great potential application for on-site testing in food and biology analysis.
研究领域:
分析化学
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生物医学工程
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纳米技术
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2023
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IF 4.4
Donglian Deng,Jingyuan Xu,Tingting Li,Dongdong Tan,Yibing Ji,Ruijun Li
This work designed a fluorometric/colorimetric dual-mode sensor for detecting 2,6-dipicolinic acid (DPA) based on the blue emission property and peroxidase-like activity of Fe-MIL-88NH 2 . The fluorescence of Fe-MIL-88NH 2 was obviously turned off by Cu 2+ , but DPA was able to bring it back because it has a strong chelate bond with Cu 2+ . Fe-MIL-88NH 2 also displayed high peroxidase-like activity, which accelerated the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue oxidation product (oxTMB) when H 2 O 2 was present. When DPA was added, it efficiently inhibited the peroxidase-like activity of Fe-MIL-88NH 2 , causing less oxTMB and less absorbance at 652 nm. The fluorescence recovery of Fe-MIL-88NH 2 and the change in absorbance at 652 nm were used as analytical signals for dual-mode detection of DPA. The linear responses in the range of 10–60 μM and 60–160 μM were achieved for the fluorometric mode, and the limit of detection (LOD) was 1.46 μM. The respective values of linear range and LOD for the colorimetric mode were 5–25 μM and 3.00 μM, respectively. In summary, the dual-mode testing strategy successfully detected DPA in aqueous environmental samples, suggesting great potential in disease prevention and environmental analysis.
研究领域:
分析化学
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环境科学
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生物传感
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DOI
PubMed
高影响因子
ANALYST
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2013
·Vol. 138
·期 16
·4526-4531
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IF 3.969
Ya Li Liu,Xi Juan Zhao,Xiao Xi Yang,Yuan Fang Li
In this paper, a nanosized porous metal–organic framework, Fe-MIL-88NH2, was facilely prepared with a uniform octahedral shape by the addition of acetic acid, and for the first time was demonstrated to possess intrinsic peroxidase-like activity. Kinetic analysis and electron spin resonance measurements indicated that the catalytic behavior was consistent with typical Michaelis–Menten kinetics and follows a ping-pong mechanism. As a novel peroxidase mimic material, Fe-MIL-88NH2 shows the advantages of high catalytic efficiency, ultrahigh stability and high biocompatibility in aqueous medium compared with natural enzymes and other peroxidase nanomimetics. Here, Fe-MIL-88NH2 was used to quickly catalyze oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a colored product, which provided a simple, sensitive and selective method for the colorimetric detection of glucose. Glucose could be linearly detected in the range from 2.0 × 10−6 to 3.0 × 10−4 M with a detection limit of 4.8 × 10−7 M, and the color variation for glucose response was also obvious by visual observation at concentrations as low as 2.0 × 10−6 M. More importantly, the colorimetric method could be successfully applied to the determination of glucose in diluted serum samples.
研究领域:
生物分析化学
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纳米生物技术
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酶模拟
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临床检验
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分子诊断
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DOI
高影响因子
ACS Applied Nano Materials
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2021
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IF 5.097
Ruya Guo,Li Xue,Gaozhe Cai,Wuzhen Qi,Yuanjie Liu,Jianhan Lin
Early screening of Salmonella is of utmost importance to minimize the incidence of related foodborne diseases. Here, we reported an immunosensor to fast detect Salmonella using Fe-MIL-88NH2 metal–organic framework (Fe-MOF) nanocubes to load platinum nanoparticles (PtNPs) for signal amplification and smartphone thermal imaging to monitor the temperature change. The Fe-MOF nanocubes decorated with PtNPs (Fe-MOF/PtNPs) were prepared as peroxidase mimics to catalyze hydrogen peroxide (H2O2) to produce O2. The immune magnetic nanoparticles (MNPs), the target bacteria, and the immune Fe-MOF/PtNPs were first mixed by the three-dimensional (3D) chaotic micromixer and conjugated in the microfluidic chip to form MNP–bacteria–Fe-MOF/PtNPs sandwich complexes. After magnetic separation and washing, H2O2 was injected and decomposed by Fe-MOF/PtNPs on the sandwich complexes to generate O2 in a sealed chamber. The increase in the pressure pushed the preloaded water to react with calcium oxide powder, resulting in the generation of heat, which was measured using the smartphone-based thermal sensor to determine the bacteria. The microfluidic immunosensor could detect Salmonella as low as 93 CFU/mL within 1 h.
研究领域:
生物传感器
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纳米技术
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微流控技术
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食品安全
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生物医学工程
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2014
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IF 2.129
Zhengjuan Sun,Yali Liu,Yuanfang Li
A novel and rapid spectrofluorometry method for the recognition of 6-mercaptopurine (6-MP) has been developed based on luminescent metal–organic frameworks Fe-MIL-88NH 2 as fluorescent probe. The strong fluorescence of Fe-MIL-88NH 2 at 430 nm could be quenched by 6-MP directly, and the Fe-MIL-88NH 2 shows high selectivity for 6-MP compared to other thiol-containing amino acids such as homocysteine (Hcy), cysteine (Cys), glutathione (GSH), etc. Under optimal conditions, the relative fluorescence intensity was linearly proportional to the concentration of 6-MP in the range of 5–600 μM with the detection limit at 1.17 μM (S/N = 3). Furthermore, the present approach has been successfully applied to the determination of 6-MP in human serum samples. The possible fluorescence quenching mechanism has also been investigated, where it is revealed that the quenching was attributed to competition of absorption of the light source energy as well as electron transfer between Fe-MIL-88NH 2 and 6-MP.
研究领域:
分析化学
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药物分析
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 9
·3121-3130
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IF 4.58
Yuan Guolin,Wang Lei,Mao Dayong,Wang Fang,Zhang Jicai
The β1 receptor blocker metoprolol targets the hypertension-carrying adrenergic receptor gene (ADRB1), but therapy is often hindered by a lack of evidence-based medical information. The authors describe an assay for the ADRB1 gene. It is based on the electrocatalytic oxidation of H 2 O 2 by a nanocomposite consisting of hemin and platinum nanoparticles in an amino-modified metal-organic framework of the type Fe-MIL-88-NH 2 and heavily loaded with Cu(II) ions. The nanocomposite was placed on a glassy carbon electrode (GCE), and the resulting sensor displays increased electrocatalytic activity with the addition of H 2 O 2 because of the synergistic effects of hemin and the platinum nanoparticles (Pt NPs). Next, a capture DNA probe for the target ADRB1 gene was immobilized on the modified GCE. Binding of the target gene results in a distinct change in the electrocatalytic activity of H 2 O 2 . The amperometric i–t curves were recorded at −0.4 V in 5 mL of phosphate buffered saline at room temperature. The electrode responds to ADRB1 in the 1 f. to 10 nM concentration range and has a 0.21 f. detection limit (at a S/N ratio of 3). In our perception, this detection scheme can simplify and accelerate the identification of drug response and therapeutic efficacy. Conceivably, it may be applied to other mutations, and it may enable a more precise clinical prognosis and thus facilitate personalized medical diagnostics.
Schematic presentation of an electrode for the determination of the adrenergic receptor gene (ADRB1). The assay is based on the electrooxidation of added H 2 O 2 by using a glassy carbon electrode (GCE) coated with a composite prepared from platinum nanoparticles (Pt NPs), hemin and a metal-organic framewok of type Fe-MIL-88NH 2 loaded with Cu(II). This versatile platform simplifies studies on the response of hypertension drugs and the effectiveness of therapy.
研究领域:
生物化学
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医学诊断
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纳米材料科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Jia Ding,Fei Liu,Chunling Qi,Yunlei Zhou,Huanshun Yin,Shiyun Ai
A novel photoelectrochemical (PEC) biosensor was constructed for DNA hydroxymethylation detection based on Bi 4 NbO 8 Cl/PTC-NH 2 heterojunction and Fe-MIL-88NH 2 , where Bi 4 NbO 8 Cl/PTC-NH 2 was employed as photoactive material and Fe-MIL-88NH 2 was adopted as artificial hydrogen peroxide mimic enzyme. Based on the structure of hydroxymethylated cytosine in DNA sequence, 5-hydroxymethyl-2’-deoxycytidine (5hmdC) was employed as detected target molecule for the first time using PEC technique. After Bi 4 NbO 8 Cl/PTC-NH 2 heterojunction was modified on the substrate electrode, thioglycolic acid (TGA) was captured by forming amide bond, where the sulfhydryl group (-SH) of TGA was employed as the specific recognition of 5hmdC. Under M. HhaI catalysis, the −CH 2 OH of 5hmdC reacted selectively with the –SH. Finally, Fe-MIL-88NH 2 was modified on the electrode based on the crosslinker of 4-formylphenylboronic acid (FPBA), where FPBA possessed two active groups of formyl and boronic acid group, reacting with glycosyl of 5hmdC and amino of Fe-MIL-88NH 2 . Under the catalysis effect of Fe-MIL-88NH 2 , the insoluble substance was produced on the surface of electrodes, resulting in distinct lessening of PEC response. Under optimal conditions, the photocurrent shows a linear relationship with the logarithm value of 5hmdC concentration from 0.01-100 nM with a low detection limit of 6.48 pM. More importantly, this method can be applied to investigate the influence of exogenous phytohormone on 5hmdC content in the genomic DNA of rice seedling roots and leaves.
研究领域:
材料科学
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分析化学
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生物传感器
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生物化学
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纳米技术
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电化学
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基因组学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）