Yang Shuaikang,Wang Weiqiang,Xu Yanan,Yuan Yonghui,Hao Shengzhi
Fe-based biodegradable materials have attracted significant attention due to their exceptional mechanical properties and favorable biocompatibility. Currently, research on Fe-based materials mainly focuses on regulating the degradation rate. However, excessive release of Fe ions during material degradation will induce the generation of reactive oxygen species (ROS), leading to oxidative stress and ferroptosis. Therefore, the control of ROS release and the improvement of biocompatibility for Fe-based materials are very important. In this study, new Fe–Zn alloys were prepared by electrodeposition with the intention of using Zn as an antioxidant to reduce oxidative damage during alloy degradation. Initially, the impact of three potential degradation ions (Fe2+, Fe3+, Zn2+) from the Fe–Zn alloy on human endothelial cell (EC) activity and migration ability was investigated. Subsequently, cell adhesion, cell activity, ROS production and DNA damage were assessed at various locations surrounding the alloy. Finally, the influence of different concentrations of Zn2+ in the medium on cell viability and ROS production was evaluated. High levels of ROS exhibited evident toxic effects on ECs and promoted DNA damage. As an antioxidant, Zn2+ effectively reduced ROS production around Fe and improved the cell viability on its surface at a concentration of 0.04 mmol/l. These findings demonstrate that Fe–Zn alloy can attenuate the ROS generated from Fe degradation thereby enhancing cytocompatibility.
Yanan Xu,Weiqiang Wang,Fengyun Yu,Shuaikang Yang,Yonghui Yuan,Yinong Wang
Pure Fe is a potential biodegradable stent material due to its better biocompatibility and mechanical properties , but its degradation rate needs to be improved. Alloying with Zn to form Fe-Zn alloy is anticipated to meet the degradation rate requirements while retaining the iron's inherent properties. Therefore, Fe-Zn alloys with monolayered and multilayered structures were prepared by electrodeposition . The alloys' composition, microstructure, mechanical properties, in vitro degradation and biocompatibility were assessed. Results showed that the Zn content ranged from 2.1 wt% to 11.6 wt%. After annealing at 450°C, all the alloys consisted of α(Fe) solid solution and Zn-rich B2 ordered coherent phase, except for the alloy with 11.6 wt% Zn content, in which a Fe 3 Zn 10 phase appeared. The layered structure consisted of alternating columnar-grain and nano-grain layers, which compensated for the intrinsic brittleness of electrodeposited metals and improved the galvanic effect of the alloy, thus increasing the strength and plasticity and changing the corrosion from localized to uniform while augmenting the corrosion rate. The yield strength of the multilayered alloy exceeded 350 MPa, its elongation was more than 20%, and its corrosion rate obtained by immersion test in Hank's solution reached 0.367 mm·y −1 . Fe-Zn alloys with lower Zn content had good cytocompatibility with the human umbilical vein endothelial cells and good blood compatibility. The above results verified that the multilayered Fe-Zn alloy prepared by electrodeposition presented enhanced mechanical properties, higher degradation rate, uniform degradation mechanism and good biocompatibility. It should be qualified for the application of biodegradable stents. Statement of significance A potential biodegradable Fe-Zn alloy, which is difficult to be obtained by the metallurgical method, was prepared by electrodeposition to solve the low degradation rate of iron-based biomaterials. A multilayered microstructure design composed of alternating columnar-grain and nano-grain layers was achieved by changing the electrical parameters. The layered design compensated for the intrinsic poor plasticity of electrodeposited metals. It increased the galvanic effect of the alloy, thus augmenting the corrosion rate and changing the corrosion mode of the alloy from localized to uniform corrosion. The yield strength of multilayered alloy exceeded 350 MPa; its elongation was more than 20%. Moreover, the layered alloy had good cytocompatibility and blood compatibility. It indicates that the alloy is qualified for biodegradable stent application.
危险品化学品经营许可证（带存储）