Materials
·
2024
·Vol. 17
·期 2
·509
·
IF 3.1
Shuya Ning,Shuo Wang,Zhihui Liu,Naming Zhang,Bin Yang,Fanghui Zhang
Magnetic–plasmonic nanoparticles (NPs) have attracted great interest in many fields because they can exhibit more physical and chemical properties than individual magnetic or plasmonic NPs. In this work, we synthesized Au- or Ag-decorated Fe3O4nanoparticles coated with PEI (Fe3O4-PEI-M (M = Au or Ag) NPs) using a simple method. The influences of the plasmonic metal NPs’ (Au or Ag) coating density on the magnetic and plasmonic properties of the Fe3O4-PEI-M (M = Au or Ag) NPs were investigated, and the density of the plasmonic metal NPs coated on the Fe3O4NPs surfaces could be adjusted by controlling the polyethyleneimine (PEI) concentration. It showed that the Fe3O4-PEI-M (M = Au or Ag) NPs exhibited both magnetic and plasmonic properties. When the PEI concentration increased from 5 to 35 mg/mL, the coating density of the Au or Ag NPs on the Fe3O4NPs surfaces increased, the corresponding magnetic intensity became weaker, and the plasmonic intensity was stronger. At the same time, the plasmonic resonance peak of the Fe3O4-PEI-M (M = Au or Ag) NPs was red shifted. Therefore, there was an optimal coverage of the plasmonic metal NPs on the Fe3O4NPs surfaces to balance the magnetic and plasmonic properties when the PEI concentration was between 15 and 25 mg/mL. This result can guide the application of the Fe3O4-M (M = Au or Ag) NPs in the biomedical field.
研究领域:
纳米材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 18
·10743
·
IF 6.208
Teng Wang,Xi Hu,Yujun Yang,Qing Wu,Chengdian He,Xiong He,Zhenyu Wang,Xiang Mao
Metal-based enzyme mimics are considered to be acceptable agents in terms of their biomedical and biological properties; among them, iron oxides (Fe3O4) are treated as basement in fabricating heterogeneous composites through variable valency integrations. In this work, we have established a facile approach for constructing Fe3O4@Ag composite through assembling Fe3O4and Ag together via polyethyleneimine ethylenediamine (PEI) linkages. The obtained Fe3O4@PEI@Ag structure conveys several hundred nanometers (~150 nm). The absorption peak at 652 nm is utilized for confirming the peroxidase-like activity of Fe3O4@PEI@Ag structure by catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. The Michaelis–Menten parameters (Km) of 1.192 mM and 0.302 mM show the higher catalytic activity and strong affinity toward H2O2and TMB, respectively. The maximum velocity (Vmax) value of 1.299 × 10−7M∙s−1and 1.163 × 10−7M∙s−1confirm the efficiency of Fe3O4@PEI@Ag structure. The biocompatibility illustrates almost 100% cell viability. Being treated as one simple colorimetric sensor, it shows relative selectivity and sensitivity toward the detection of glucose based on glucose oxidase. By using indocyanine green (ICG) molecule as an additional factor, a remarkable temperature elevation is observed in Fe3O4@PEI@Ag@ICG with increments of 21.6 °C, and the absorption peak is nearby 870 nm. This implies that the multifunctional Fe3O4@PEI@Ag structure could be an alternative substrate for formatting acceptable agents in biomedicine and biotechnology with enzymatic and photothermal properties.Keywords:Fe3O4@PEI@Ag;assembling process;enzymatic;catalytic performance;photothermal property
研究领域:
生物医学
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材料科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2012
·Vol. 15
·期 1
·1-11
·
IF 3.287
Wang Yuanfeng,Xu Feng,Zhang Lei,Wei Xinlin
A one-step strategy for the facile synthesis of polyethyleneimine-functionalized Fe 3 O 4 (Fe 3 O 4 -PEI) composite is presented in this study. We also investigated the effects of reaction time and NaAc on the composite sizes. The composite with good dispersion, wonderful crystallization, and excellent magnetism was obtained through one-pot solvothermal reaction. In the sequential presence of PEI and colloidal gold (Au) solution, plentiful Au nanoparticles with a diameter of 5 nm were assembled on the surface of Fe 3 O 4 –PEI to get Fe 3 O 4 –PEI–Au 1 –PEI–Au 2 (Fe 3 O 4 /Au) composite. In-depth characterization of the composite formation process was performed using transmission electron microscopy, scanning electron microscope, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometer, and UV–Visible spectroscopy. The Fe 3 O 4 /Au composite was obtained with an average diameter of 150 nm, and the absorption peak was red-shifted to 580 nm. The synthesized Fe 3 O 4 /Au composite, the saturation magnetization of which was 40.4 emu/g, presented good magnetic property. Moreover, the composite could easily combine with up to 890 μg antibodies per mg, and the antibodies still kept good immunocompetence after immobilization. These advantages suggest its great potential for applications in vitro, such as separation and immunoassay.
研究领域:
材料科学
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纳米技术
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分析化学
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
Molecular Catalysis
·
2021
·
IF 5.062
Bowei Shen,Farong Zhang,Mengqi Zhao,Zhiquan Pan,Qingrong Cheng,Hong Zhou
The magnetic solid acid, Fe 3 O 4 @PEI@SO 3 H, was synthesized and used as a catalyst to transfer steroid saponins into diosgenin in an alcoholysis approach. The results showed that the magnetic solid acid greatly improved the alcoholysis efficiency for extraction diosgenin from steriod saponins. To optimize the parameters for alcoholysis, such as the mass of the solid acid, solvent volume, the reaction temperature and time, single factor experiments as well as response surface experiment have been performed. Under the optimal conditions, the diosgenin yield obtained by alcoholysis is higher than that of the traditional inorganic acid hydrolysis. And it worth to mention that no waste liquid is discharged. The catalyst is very stable and can be used for three times without obvious mass loss and activity decrease, indicating that the technology of extracting diosgenin from Dioscorea zingiberensis C.H. Wright tuber has a broad prospect in industrial application.
研究领域:
生物化学
·
有机合成
·
制药工程
✓ 已验证
DOI
高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
·
2021
·Vol. 53
·期 2
·211-221
·
IF 3.133
Nan Jia,Yinran Xiong,Ying Wang,Siqian Lu,Ruoqiu Zhang,Yan Kang,Yiping Du
A label-free and novel surface-enhanced Raman scattering (SERS) method has been studied to rapidly detect common fish pathogenic bacteria ( Escherichia coli , Vibrio anguillarum , Vibrio harveyi , Edwardsiella piscicida , and Pseudomonas plecoglossicida ). The marriage of magnetic nanospheres (Fe 3 O 4 ) with polyethylenimine (PEI) could trigger surface charge modifications of Fe 3 O 4 , making it effective to capture negatively charged bacteria. After the rapid bacterial capture procedure within 10 min, Au@Ag core-shell bimetallic nanoparticles served as hotspot providers to facilitate the enhancement of biological Raman signals. The results demonstrated that the minimum detection limit of E. piscicida could be as low as 10 5 cfu/ml. The peak intensity at 1628 cm −1 ( E. piscicida ) showed a linear relationship with the logarithm of the bacterial concentration ( R 2 = 0.997). A mimic test was conducted by spiking E. piscicida into tap water. Linear discriminant analysis (LDA) was also harnessed to classify different types of fish bacteria. And the positive rates of the training set and the prediction set were 100% and 80%, respectively. The whole experimental procedure only takes 20 min. This method opens a new avenue for the rapid detection and identification of fish pathogenic bacteria.
研究领域:
水产养殖学
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生物分析化学
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纳米技术
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Ziwei Liang,Yuhui Wang,Jiapu Wang,Tao Xu,Shilong Ma,Qi Liu,Liqin Zhao,Yan Wei,Xiaojie Lian,Di Huang
Ferroptosis is a novel form of regulated cell death induced by iron-dependent lipid peroxidation imbalance. It has emerged as a promising antitumor therapeutic strategy in recent years. In this work, we successfully synthesized a complex magnetic nanocube Fe 3 O 4 modified with PEI and HA by the thermal decomposition method. While loading a ferroptosis inducer RSL3 inhibited cancer cells through the ferroptosis signal transduction pathway. The drug delivery system could actively target tumor cells through an external magnetic field and HA-CD44 binding. Zeta potential analysis showed that Fe 3 O 4 [email protected] nanoparticles were more stable and uniformly dispersed in tumor acidic environment. Moreover, cellular experiments demonstrated that Fe 3 O 4 [email protected] nanoparticles could significantly inhibit the proliferation of hepatoma cells without a cytotoxic effect on normal hepatic cells. In addition, Fe 3 O 4 [email protected] played a vital role in ferroptosis by accelerating ROS production. The expression of ferroptosis-related genes Lactoferrin, FACL 4, GPX 4 and Ferritin was significantly suppressed with increasing treatment of Fe 3 O 4 [email protected] nanocubes. Therefore, this ferroptosis nanomaterial has great potential in Hepatocellular carcinoma (HCC) therapy.
研究领域:
纳米技术
·
癌症生物学
·
药学
·
分子生物学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Kailu Wang,Jie Wen,Suiwang Zhang,Lingzhi Yang,Huan Yang,Xiaorong Yu,Hui Zhang
The polyacrylamide-based gel used for oilfield conformance control exhibits poor temperature and salt resistance. This paper proposes the use of inorganic-organic core-shell materials as chemical crosslinkers for the polyacrylamide-polyethyleneimine (PAM-PEI) gel system to regulate and design its gel structure, enabling it to withstand harsh reservoir conditions. To achieve this, Fe 3 O 4 @PEI nanoparticles were prepared using a facile one-pot method and employed as chemical crosslinkers for the PAM-PEI gel. Herein, the Fe 3 O 4 "core" provides strong stability derived from the inorganic material, and the PEI "shell" reacts with the PAM molecular chain in a transamidation reaction, so that the Fe 3 O 4 @PEI nanoparticles are riveted inside the gel by chemical crosslinking, which greatly enhances the gel structure. The Fe 3 O 4 @PEI nanoparticles exhibit a nearly spherical shape with an average diameter of approximately 75.6 nm and demonstrate excellent dispersion in water, possessing a zeta potential of 40.8 mV. In comparison to the control PAM-PEI gel, the PAM-PEI-Fe 3 O 4 @PEI gel exhibited significantly enhanced gel strength, rheological properties, long-term thermal stability, and anti-salt properties. It demonstrated a tan(δ) value of 0.12, indicative of its behavior as a nearly robust gel. Even after 360 days of placement at 90 °C in a 20 g/L NaCl solution, the gel maintained its Sydansk code at code I . Moreover, NaCl was observed to act as a retarder for this gel, while its Sydansk strength remained unaffected. In addition, the PAM-PEI-Fe 3 O 4 @PEI gel is magnetically responsive, so it can be utilized with a magnet generator to enhance its plugging performance and control its directional movement. By monitoring the magnetization rate of the gel in the reservoir, high permeability layers can be accurately identified, thus providing important practical applications in reservoir management.
研究领域:
材料科学
·
石油工程
·
化学工程
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Dan-Dan Zhang,Zhi-Yong Guo,Peng-Fei Guo,Xue Hu,Xu-Wei Chen,Jian-Hua Wang
Polyoxometalate [{a-PW11O39Zr(μ-OH)(H2O)}2]8– (POM1) is first prepared by sandwiching ZrIV among 2 mono-lacunary α-Keggin polyoxometalates, and then novel magnetic nanoparticles (NPs), Fe3O4@polyethyleneimine (PEI)@POM1, are fabricated by coating POM1 onto the surface of magnetic Fe3O4@PEI NPs under electrostatic interaction. The obtained Fe3O4@PEI@POM1 NPs are characterized by Fourier transform infrared, zeta potential, vibrating sample magnetometer, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction. Ascribed to the hydrogen-bonding and electrostatic interactions, the NPs exhibit high adsorption selectivity toward IgG, and the adsorption capacity is high up to 304 mg g–1 under optimal adsorption conditions. By using 0.01% cetyl trimethylammonium bromide to strip the adsorbed protein species, an elution efficiency of 95% is achieved. The feasibility of Fe3O4@PEI@POM1 NPs in real-world sample assay has been demonstrated by the selective isolation of IgG heavy chain and light chain from human serum, as confirmed by the sodium dodecyl sulfate polyacrylamide gel electrophoresis assay.
研究领域:
纳米技术
·
材料科学
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2025
·
IF 6.3
Dajun Zhang,Yufei Ma,Ying Wang,Xiangyu Liu,Mengfan Zhao,Yuanke Wang,Song Gao,Fu Ren,Qing Chen
A new solid-phase extraction agent, IgG@ Fe 3 O 4 -PEI, was successfully developed by conjugating immunoglobulin G (IgG) with amino-functionalized magnetic nanoparticles (Fe 3 O 4 -PEI). The IgG@Fe 3 O 4 -PEI composite was characterized using various techniques including infrared spectroscopy, Raman spectroscopy, magnetic property testing, Zeta potential measurements, energy spectrum analysis, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Because of the presence of a specific binding domain in the Fc fragment of IgG, the IgG@Fe 3 O 4 -PEI composite demonstrated highly selective adsorption of recombinant streptococcal G protein (rSPG) under optimal experimental conditions. At pH 6.0, the IgG@Fe 3 O 4 -PEI composite (0.5 mg) achieved a maximum adsorption efficiency of 93.96 % for rSPG at a concentration of 100 μg mL -1 . Adsorption model simulations indicated that the adsorption behavior followed the Langmuir adsorption model, with a theoretical maximum adsorption capacity of 125.0 mg g -1 . The enriched rSPG was eluted and recovered using Britton-Robinson (BR) buffer at pH 10.0, achieving a recovery efficiency of 95.8 %. In practical applications, the IgG@Fe 3 O 4 -PEI magnetic nanoparticles effectively enriched rSPG from an Escherichia coli expression system, and SDS-PAGE analysis confirmed the successful isolation of highly pure rSPG. Furthermore, the IgG@Fe 3 O 4 -PEI magnetic nanoparticles were used to capture proteins from human serum samples. After enrichment and processing, 430 proteins were identified, including 34 that were undetectable in untreated serum samples. This validated the high sensitivity and specificity of this method in serum proteomics, demonstrating its potential for isolating and identifying low-abundance proteins in complex biological samples. The developed system offers a valuable tool for advancing human serum proteomics research and targeted protein analysis.
研究领域:
生物化学
·
纳米技术
·
蛋白质组学
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Jundong Wang,Huiyuan Guan,Qiang Han,Siyuan Tan,Qionglin Liang,Mingyu Ding
In this work, Yb3+-immobilized hydrophilic phytic-acid-coated magnetic nanocomposites were prepared through a facile route and used to selectively separatrf bovine hemoglobin. Hydrophilic phytic acid (PA) was coated onto the magnetic Fe3O4-PEI via electrostatic interactions, followed by finally chelating with Yb3+ ions, which could produce specific protein binding sites at room temperature in water, and complex instrumentation was not necessary. The performance of as-prepared hybrids (Fe3O4-PEI-PA-Yb3+) was assessed by selectively isolating bovine hemoglobin (BHb). The obtained maximum binding capacity was 347.3 mg g–1. The retained BHb could be eluted under simple elution via using 0.1 M of Na2CO3, giving a recovery of 83%. Moreover, the generation of nanocomposites was demonstrated. In addition, the PA and PEI could improve the hydrophilicity of nanoparticles and further reduce the nonspecific adsorption. Therefore, such nanocomposites were successfully employed to selectively bind and separate BHb from bovine serum as verified by SDS-PAGE and MALDI-TOF MS analysis, providing a new perspective for the isolation of heme proteins in proteomics.
研究领域:
纳米技术
·
蛋白质组学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2018
·
IF 0
Changfen Bi,Yulu Liang,Lijin Shen,Shanshan Tian,Kai Zhang,Yiliang Li,Xiwen He,Langxing Chen,Yukui Zhang
Efficient enrichment glycoproteins/glycopeptides from complex biological solutions are very important in the biomedical sciences, in particular biomarker research. In this work, the high hydrophilic polyethylenimine conjugated polymaltose polymer brushes functionalized magnetic Fe3O4 nanoparticles (NPs) denoted as Fe3O4–PEI–pMaltose were designed and synthesized via a simple two-step modification. The obtained superhydrophilic Fe3O4–PEI–pMaltose NPs displayed outstanding advantages in the enrichment of N-linked glycopeptides, including high selectivity (1:100, mass ratios of HRP and bovine serum albumin (BSA) digest), low detection limit (10 fmol), large binding capacity (200 mg/g), and high enrichment recovery (above 85%). The above-mentioned excellent performance of novel Fe3O4–PEI–pMaltose NPs was attributed to graft of maltose polymer brushes and efficient assembly strategy. Moreover, Fe3O4–PEI–pMaltose NPs were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 449 N-linked glycopeptides, representing 323 different glycoproteins and 476 glycosylation sites, were identified. It was expected that the as-synthesized Fe3O4–PEI–pMaltose NPs, possessing excellent performance (high binding capacity, good selectivity, low detection limit, high enrichment recovery, and easy magnetic separation) coupled to a facile preparation procedure, have a huge potential in N-glycosylation proteome analysis of complex biological samples.
研究领域:
生物医学科学
·
蛋白质组学
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纳米技术
·
分析化学
✓ 已验证
DOI
PubMed
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