JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Dandan Li,Xiaoyu Wang,Chen Xie,Ziwu Fan,Rui Ding,Songqi Liu,Yu Chen,Kai Yu
The widespread co−existence of triphenyl phosphate (TPhP) and heavy metals in sediments raises significant concerns. However, uncertainty still exists regarding the degradation characteristics of TPhP in the presence of cadmium (Cd). Therefore, this research was conducted to elucidate the impacts of Cd on TPhP degradation in sediments. Results showed that the biodegradation efficiency of TPhP in sediments exceeded 90 %. The identified degradation products of TPhP indicated that the degradation pathway primarily involved oxidative cleavage, hydroxylation, and methylation, which was further supported by condensed dual descriptors (CDD). However, the presence of Cd significantly inhibited TPhP degradation, with greater inhibition at higher concentrations of both TPhP and Cd. Transcriptomic analysis revealed that xenobiotic metabolism was significantly suppressed. Furthermore, pathways associated with cell motility, membrane transport, and energy metabolism were impaired. These disruptions likely contributed to the decreased degradation efficiency and prolonged half−life of TPhP. Interestingly, biomarkers in Firmicutes were enriched in the high−concentration TPhP + Cd treatment and strongly correlated with degradation−related gene expression, suggesting their significance in TPhP degradation under heavy metal stress. Overall, this research provides insights into the inhibitory effects of Cd on TPhP degradation and offers theoretical support for the bioremediation of TPhP−contaminated sediments with heavy metals.
研究领域:
环境科学
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分子生物学
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计算化学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
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2021
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IF 5.64
Long Gao,Hu Yuting,Tao Enfu,Chen Bo,Shu Xiaoli,Zheng Wei,Jiang Mizu
The intestinal microbiota has emerged as a critical regulator of growth and development in the early postnatal period of life. Cesarean section (CS) delivery is one of the strongest disrupting factors of the normal colonization process and has been reported as a risk factor for disorders in later life. In this study, we dynamically and longitudinally evaluated the impact of CS on the initial colonization pattern and development of gut microbiota by 16 healthy Chinese infants with fecal samples collected at 9 time points (day 5, day 8, day 11, week 2, week 4, week 6, week 7, month 2, and month 3) during the first 3 months of life. The V3–V4 regions of 16S rRNA gene were analyzed by Illumina sequencing. In comparison with vaginally delivered (VD) infants, infants born by CS showed decreased relative abundance of Bacteroides and Parabacteroides and enrichment of Clostridium_sensu_stricto_1, Enterococcus, Klebsiella, Clostridioides, and Veillonella. Most interestingly, Firmicutes/Bacteroidetes ratio was found to be significantly higher in the CS group than in the VD group from day 5 until month 3. Besides, the results of microbial functions showed that the VD group harbored significantly higher levels of functional genes in vitamin B6 metabolism at day 5, day 8, week 2, week 4, week 6, week 7, month 2, and month 3 and taurine and hypotaurine metabolism at day 5, while the phosphotransferase system and starch and sucrose metabolism involved functional genes were plentiful in the CS group at day 11, week 2, week 4, week 6, week 7, and month 2 and at week 2, week 7, and month 2, respectively. Our results establish a new evidence that CS affected the composition and development of gut microbiota in the first 3 months and provide a novel insight into strategies for CS-related disorders in later life.
研究领域:
微生物学
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儿科
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基因组学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Qianqian Jiang,Qi Chu,Qi Zhang,Chaoqiang Wu,Tongqing Tang,Xin Wang,Feifei Chen,Alei Zhang,Kequan Chen
Adol condensation mediated by L-threonine aldolase (LTA) is a promising route to synthesize β-hydroxy-α-amino acids, such as the anti-Parkinson's disease drug L- threo -3,4-dihydroxyphenylserine (L- threo -DOPS; droxidopa). However, the biocatalytic approach generally suffered from poor stereoselectivity at the C β chiral center. Herein, several LTAs from diverse microorganisms were examined for the stereoselective synthesis of L- threo -DOPS, and an LTA from Firmicutes bacterium ( Fb LTA) was identified with relatively high stereoselectivity. A semi-rational design was then employed to further enhance the stereoselectivity, resulting in the identification of beneficial mutations and the generation of beneficial mutants with markedly elevated stereoselectivity (up to 80 % de) over the wild-type (23 % de). Molecular dynamics (MD) simulation revealed that the significant improvement of stereoselectivity could be attributed to the structural reconstitution of substrate tunnels. Finally, the best mutant was applied to a scaled-up reaction, affording 1.3 g/L yield of L- threo -DOPS with 80 % de. This study provides new insights into the stereoselectivity control of LTAs and potential engineered LTAs for the manufacture of L- threo -DOPS.
研究领域:
生物化学
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合成生物学
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药物开发
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产品关联
DOI
PubMed
高影响因子
Heliyon
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2024
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IF 3.4
Tianshi Li,Mengqi Shi,Yan Zhao,Zhongmei He,Ying Zong,Weijia Chen,Rui Du
As a traditional Chinese herbal medicine, Cornu Cervi Degelatinatum (CCD) has the effect of warming the kidney to support yang, astringing, and stopping bleeding, and is used for spleen kidney yang deficient (SKYD). This experiment was to investigate the therapeutic effects of different processes of CCD on SKYD type ulcerative colitis (UC) rats and to explore its impact on the intestinal flora of rats. Methods: ELISA was used to study the anti-inflammatory activity of Cornu Cervi Degelatinatum processed with water (WCCD) and Cornu Cervi Degelatinatum processed with vinegar (VCCD). 16SrRNA and transcriptome sequencing were used to detect the composition of rat intestinal flora and gene expression; RT-PCR and Western blot were used to verify the role of WCCD and VCCD in treating UC. Results: WCCD and VCCD have therapeutic effects on UC, could reduce tissue damage. VCCD performed better in improving Bacteroidetes/Firmicutes ratios and species evenness and abundance; performed better in increasing the quantity of lactobacillus. VCCD simultaneously inhibit the intestinal inflammatory response through NCK2, PAK4, and JNK signaling pathways. Conclusions: WCCD and VCCD play a therapeutic role in UC by regulating the proportion of different flora in the intestinal flora. VCCD regulates the intestinal flora and inflammatory response by interfering with the NCK2, PAK4 and JNK signaling pathways.
研究领域:
中医中药
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药理学
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分子生物学
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胃肠病学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Fanghua Guo,Dongying Chen,Rong Tsao,Fereidoon Shahidi,Hua Xiong,Hua Zhang,Li Jiang,Yong Sun
Lentil hulls, a by-product of lentil processing, are rich in polyphenols and dietary fiber. In this study, a mouse model of nonalcoholic fatty liver disease (NAFLD) induced by dextran sulfate sodium (DSS) and high-fat diet (HFD) was used to investigate the intervention effect of green lentil hulls (GLH) from the perspective of intestinal and hepatic metabolism. Results suggest that DSS-induced gut inflammation and barrier disruption aggravate NAFLD; GLH intervention lowered body weight, liver weight gain and lipid deposition. It also inhibited liver fat accumulation and inflammation as well as intestinal inflammation. In addition, it enhanced liver antioxidant capacity, glucose tolerance and intestinal barrier integrity. Analysis of intestinal flora indicated that GLH advanced the abundance of Akkermansia-muciniphila and Verrucomicrobiaceae , which led to increased content of short-chain fatty acids (SCFAs) and inhibited the Firmicutes and Proteobacteria . Moreover, GLH intervention regulated taurine and hypotaurine metabolism, as well as the biosynthesis of unsaturated and saturated fatty acids in the liver. The relief by GLH on NAFLD may be a comprehensive effect of its rich dietary fiber and polyphenols on intestinal and hepatic metabolism via the gut-liver axis. This research also suggests that the low-value lentil hulls can serve as a valuable food supplement for enhanced gut health and reduced risk of NAFLD.
研究领域:
营养学
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胃肠病学
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肝病学
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微生物学
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代谢学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）