ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Chang Wang,Yike Wu,Sheng Liu,Liang Zhang,Bi-Feng Liu,Xin Liu
N-glycans that are fluorescently tagged by glycosylamine acylation have become a promising way for glycan biomarker discovery . Here, we describe a simple and rapid method using Fmoc N-hydroxysuccinimide ester (Fmoc-OSu) to label N-glycans by reacting with their corresponding intermediate glycosylamines produced by microwave-assisted deglycosylation . After optimizing reaction conditions, this derivatization reaction can be effectively achieved under 40 °C for 1 h. Moreover, the comparison of fluorescent intensities for Fmoc-OSu, Fmoc-Cl and 2-AA labeling strategies were also performed. Among which, the fluorescent intensities of Fmoc-OSu labeled glycan derivatives were approximately 5 and 13 times higher than that labeled by Fmoc-Cl and 2-AA respectively. Furthermore, the developed derivatization strategy has also been applied for analyzing serum N-glycans, aiming to screen specific biomarkers for early diagnosis of lung squamous cell cancer. More interestingly, the preparation of free reducing N-glycan standards have been achieved by the combination of HPLC fraction of Fmoc labeled glycan derivatives and Fmoc releasing chemistry . Overall, this proposed method has the potential to be used in functional glycomic study.
研究领域:
生物化学
·
医学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Fei Li,Xuemin Duan,Shuxin Hu,Long Zhang,Liang Shen,Fang Liu,Hui Li,Ge Zhang,Jingkun Xu
Hexavalent chromium (Cr(VI)) pollution is related to human health, and the detection of its concentration has a decisive effect on pollution treatment. In this work, we designed a Cr 2 O 7 2− sensor P(Fmoc-Osu) and applied it in farmland. In the boron trifluoride diethyl etherate solution, the monomer Fmoc-Osu was electropolymerized and obtained its conjugated polymer P(Fmoc-Osu) . The introduction of succinimide and carbonic ester group in side chain enabled it to show high selectivity to Cr 2 O 7 2− in comparison to anions and twenty amino acids. Furthermore, by taking advantage of the signal amplification effect of a conjugated polymer, P(Fmoc-Osu) could analyze Cr 2 O 7 2− in trace amounts and the limit of detection was 8.3 fM. For agricultural application, we employed it in farmland water, soil, Chinese cabbage, carrot and the results showed a satisfactory result. All these results showed that P(Fmoc-Osu) is an efficient tool to detect Cr 2 O 7 2− in practical application.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2015
·
IF 3.088
Xiao Gong,Christopher Branford-White,Lei Tao,Shubai Li,Jing Quan,Huali Nie,Limin Zhu
Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology. In the present study, we dedicate to fabricate a rigid and structure controllable Fmoc-FF/SA composite hydrogel. We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration. Meanwhile, CD-spectroscopy demonstrated that SA appeared to control the process, but it did not change the arrangement of the Fmoc-FF peptide. Our results demonstrated that the formed hydrogel showed physical and chemical stability as well as possessing good biocompatibility. Rheological measurements showed that the addition of SA could improve the stability of the hydrogel. Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro . Our results indicated that the fabricated Fmoc-FF/SA composite hydrogels could be used in tissue engineering and drug delivery in the future.
研究领域:
生物材料学
·
纳米技术
·
组织工程
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2016
·Vol. 40
·期 6
·5568-5576
·
IF 3.277
Yimeng Zhang,Shangyang Li,Mingfang Ma,Minmin Yang,Yajie Wang,Aiyou Hao,Pengyao Xing
Gel formation by an N-fluorenyl-9-methoxycarbonyl (Fmoc)-based molecule was investigated and the chirality amplification observed during the self-assembly process could be switched on and off by controlling the solvent environment. The molecules could form gel in mixed solvents of water and dimethyl sulfoxide (DMSO) at either a high water fraction to afford flat nanofibers or a low water fraction to generate nanofibers with supramolecular chirality. At a moderate fraction of water, the molecules self-assemble into a precipitate composed of nanosheets. Furthermore, the gels showed different mechanical properties and thermal stabilities. Mechanism studies showed that π–π stacking interactions between the aromatic Fmoc groups and hydrogen bondings between the amide groups played important roles in the self-assembly process of the different gels. This study strives to shed light on the tuning of chiral gels with various nanostructures via controlled self-assembly, which might have potential use in smart materials.
研究领域:
材料科学
·
化学
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 15
·2629
·
IF 5.1
Xian-Ni Su,Yu-Yang Wang,Muhammed Fahad Khan,Li-Na Zhu,Zhong-Liang Chen,Zhuo Wang,Bing-Bing Song,Qiao-Li Zhao,Sai-Yi Zhong,Rui Li
Background:During food processing and storage, traditional protein-based delivery systems encounter significant challenges in maintaining the structural and functional integrity of bioactive compounds, primarily due to their temporal instability.Methods:In this study, a nanocomposite hydrogel was prepared through the co-assembly of a self-assembling peptide, 9-Fluorenylmethoxycarbonyl-phenylalanine-arginine-glycine-aspartic acid-phenylalanine (Fmoc-FRGDF), and hyaluronic acid (HA). The stability of this hydrogel as a quercetin (Que) delivery carrier was systematically investigated. Furthermore, the impact of Que co-assembly on the microstructural evolution and physicochemical properties of the hydrogel was characterized. Concurrently, the encapsulation efficiency (EE%) and controlled release kinetics of Que were quantitatively evaluated.Results:The findings indicated that HA significantly reduced the storage modulus (G′) from 256.5 Pa for Fmoc-FRGDF to 21.1 Pa with the addition of 0.1 mg/mL HA. Despite this reduction, HA effectively slowed degradation rates; specifically, residue rates of 5.5% were observed for Fmoc-FRGDF alone compared to 14.1% with 0.5 mg/mL HA present. Notably, Que enhanced G′ within the ternary complex, increasing it from 256.5 Pa in Fmoc-FRGDF to an impressive 7527.0 Pa in the Que/HA/Fmoc-FRGDF hydrogel containing 0.1 mg/mL HA. The interactions among Que, HA, and Fmoc-FRGDF involved hydrogen bonding, electrostatic forces, and hydrophobic interactions; furthermore, the co-assembly process strengthened the β-sheet structure while significantly promoting supramolecular ordering. Interestingly, the release profile of Que adhered to the Korsmeyer–Peppas pharmacokinetic equations.Conclusions: Overall, this study examines the impact of polyphenol on the rheological properties, microstructural features, secondary structure conformation, and supramolecular ordering within peptide–polysaccharide–polyphenol ternary complexes, and the Fmoc-FRGDF/HA hydrogel system demonstrates a superior performance as a delivery vehicle for maintaining quercetin’s bioactivity, thereby establishing a multifunctional platform for bioactive agent encapsulation and controlled release.
研究领域:
药剂学
·
材料科学
·
食品科学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Soft Matter
·
2014
·Vol. 10
·期 48
·9695-9701
·
IF 4.151
Zupeng Huang,Quan Luo,Shuwen Guan,Jianxiong Gao,Yongguo Wang,Bo Zhang,Liang Wang,Jiayun Xu,Zeyuan Dong,Junqiu Liu
Artificial enzymes capable of achieving tunable catalytic activity through stimuli control of enzymatic structure transition are of significance in biosensor and biomedicine research. Herein we report a novel smart glutathione peroxidise (GPx) mimic with modulatory catalytic activity based on redox-induced supramolecular self-assembly. First, an amphiphilic Fmoc-phenylalanine-based selenide was designed and synthesized, which can self-assemble into nanospheres (NSs) in aqueous solution. The NSs demonstrate extremely low GPx activity. Upon the oxidation of hydroperoxides (ROOH), the selenide can be quickly transformed into the selenoxide form. The change of the molecular structure induces complete morphology transition of the self-assemblies from NSs to nanotubes (NTs), resulting in great enhancement in the GPx catalytic activity. Under the reduction of GSH, the selenoxide can be further reversibly reduced back into the selenide; therefore the reversible switch between the NSs and NTs can be successfully accomplished. The relationship between the catalytic activity and enzymatic structure was also investigated. The dual response nature makes this mimic play roles of both a sensor and a GPx enzyme at the same time, which can auto-detect the signal of ROOH and then auto-change its activity to achieve quick or slow/no scavenging of ROOH. The dynamic balance of ROOH is vital in organisms, in which an appropriate amount of ROOH does benefit to the metabolism, whereas surplus ROOH can cause oxidative damage of the cell instead and this smart mimic is of remarkable significance. We expect that such a mimic can be developed into an effective antioxidant drug and provide a new platform for the construction of intelligent artificial enzymes with multiple desirable properties.
研究领域:
生物化学
·
仿生材料
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Today
·
2026
·
IF 10.7
Yan Zhang,Lingxin Peng,Baotong Ye,Guodong Lu,Sichen Li,Jingjing Zhou,Mihail Barboiu,Jinghua Chen
Nanoparticles (NPs) have been extensively investigated in modern cancer chemotherapy for their advantages in targeted drug delivery, enhanced efficacy and reduced toxicity. However, clinical evidence continues to indicate modest therapeutic efficacy of these nanomedicines, largely attributed to insufficient drug accumulation and poor tissue penetration in tumors. In the current study, we discovered that synergetic and precise control over nanoparticle shape and surface functionalization improves tumor penetration and enhances chemotherapy outcomes. Specifically, supramolecular nanospheres/nanorods self-assembled from scaffolding Fmoc-FF, hepatocyte targeting Fmoc-monosaccharides and the anti-tumor drug doxorubicin (DOX) have been associated via the synergetic control of their nanostructured morphologies and the surface presentation of bioactive signals. These pH-sensitive self-assembled nanoparticles enable controlled nanostructure shape and multivalent carbohydrate-functionalized surfaces, allowing activation in the acidic tumor microenvironment for triggered drug release. In vitro experiments demonstrated that the galactose-modified nanorod FGal 1–2 , measuring approximately 250 nm in length and of 25 nm in diameter, displayed the highest toxicity towards HepG2 cells and significantly enhanced cellular uptake. Additionally, nanorod FGal 1–2 demonstrated the deepest penetration (> 80 μm) in a 3D tumor spheroid model, surpassing the nanosphere FGal 1–1 and longer nanorod FGal 1–3 . Moreover, in vivo animal studies further revealed higher tumor accumulation of nanorod FGal 1–2 compared to the corresponding nanosphere, along with the strongest tumor inhibition, markedly reduced cardiotoxicity of DOX, and an 85% reduction in tumor volume. This work offers new insights into the design of nanoparticles with functional/morphological effects in their properties for advanced anti-tumor drug delivery.
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DOI
高影响因子
AMINO ACIDS
·
2023
·Vol. 55
·期 4
·499-507
·
IF 3.5
Qin Tiansheng,Huang Xiande,Zhang Qianqian,Chen Fan,Zhu Jiaojiao,Ding Yaoyao
During trauma and surgery, bleeding is a major concern. One of the crucial strategies for hemostasis is the use of biological hemostatic material. Herein, we reported an amino acid-based hydrogel FmocF-ADP hydrogel, which consisted of N-[(9H-fluoren-9-ylmethoxy) carbonyl]-3-phenyl-L-alanine (FmocF) and adenosine diphosphate (ADP) sodium solution. The hydrogel was created by FmocF self-assembling to nanofiber in ADP sodium solution and then cross-linking to hydrogel. FmocF-ADP hydrogel showed good in vitro coagulation activity as measured by whole blood clotting assays, platelet clotting assays, platelet activation assays, and platelet adhesion assays. Further, it was noted to reveal an exceptional in vivo hemostatic effect in a mouse liver bleeding model. Together with the previous report of the good biocompatibility and antimicrobial activity of FmocF hydrogel, our study would extend the biomedical application of FmocF hydrogel. In conclusion, the present study would provide a constructive strategy for the development of new antimicrobial and hemostatic materials or develop a potential hemostatic material.
研究领域:
生物医学工程
·
材料科学
·
止血
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
CHEMPHYSCHEM
·
2014
·Vol. 15
·期 11
·2377-2385
·
IF 3.36
Pengyao Xing,Xiaoxiao Chu,Shangyang Li,Mingfang Ma,Aiyou Hao
Graphical Well gel: A supramolecular gel that is robust and rigid with high elastic modulus and yield stress is fabricated by using a good/poor solvent strategy. Driven by intermolecular H-bonds, the ultralow-molecular-weight gelator forms a lamellar gel, which then combines with graphene oxide (GO) sheets to form supramolecular networks. The interactions between native gels and GO sheets are efficient, and the mechanical properties, fluorescent emission, and thermal properties can be tailored flexibly. A supramolecular gel is obtained from the self-assembly of an ultralow-molecular-weight gelator ( N -fluorenyl-9-methoxycarbonyl glutamic acid) in good and poor solvents. The gelators can self-assemble into a lamellar structure, which can further form twisted fibers and nanotubes in the gel phase. Rheological studies show that the gels are robust and rigid, and are able to rapidly self-recover to a gel after being destroyed by shear force. Fluorescence experiments reveal the aggregation-induced emission effects of the gel system; the fluorescence intensity is significantly enhanced by gel formation. Graphene oxide (GO) is introduced into the system efficiently to give a hybrid material, and the interaction between gelators–GO sheets is studied. Rheological and fluorescent studies imply that the mechanical properties and the fluorescent emission of the hybrid materials can be fine-tuned by controlling the addition of GO.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale Research Letters
·
2016
·Vol. 11
·期 1
·1-9
·
IF 2.584
Xie Yanyan,Zhao Jun,Huang Renliang,Qi Wei,Wang Yuefei,Su Rongxin,He Zhimin
We report a new approach to constructing a peptide–polysaccharide hybrid hydrogel via the calcium-ion-triggered co-assembly of fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF) peptide and alginate. Calcium ions triggered the self-assembly of Fmoc-FF peptide into nanofibers with diameter of about 30 nm. Meanwhile, alginate was rapidly crosslinked by the calcium ions, leading to the formation of stable hybrid hydrogel beads. Compared to alginate or Fmoc-FF hydrogel alone, the hybrid Fmoc-FF/alginate hydrogel had much better stability in both water and a phosphate-buffered solution (PBS), probably because of the synergistic effect of noncovalent and ionic interactions. Furthermore, docetaxel was chosen as a drug model, and it was encapsulated by hydrogel beads to study the in vitro release behavior. The sustained and controlled docetaxel release was obtained by varying the concentration ratio between Fmoc-FF peptide and alginate.
研究领域:
生物材料学
·
药剂学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Mengmeng Feng,Huifang Ge,Ting Zhang,Jingbo Liu,Boqun Liu,Yali Yu
The quantification and analysis of free amino acids (FAAs) in serum is important for illness diagnosis due to its content varies with the body’s condition. In this study, a new sensitive analytical method based on liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS/MS) was optimized for simple and rapid simultaneous detection of 20 amino acids by pre-column derivatization. Amino acid derivatized by 9-fluorenyl methyl chloroformate (FMOC-Cl) were eluted and separated by simple mobile phase reagent in 20 min via gradient elution using a traditional reverse phase C18 column (150 × 4.6 mm, 5 μm), at the flow rate of 0.8 mL/min. After optimizing derivatization, LC-MS/MS conditions, and sample pre-treatment, this method was verified with LODs and LOQs ranging from 0.0237 to 0.2391 ng/mL and 0.079 to 0.7971 ng/mL. The intra- and inter-day precision was no more than 10 %. The overall accuracy and recovery varied from 88.4-111 % and 88.7–114 %, respectively. Those methods were first applied to mice serum with ulcerative colitis. The results showed that the levels of Ala, Arg, Trp, Lys, Cys, Tyr, and Phe significantly increased in pre-treated group by egg white peptides, which will participate in metabolism. This study provides a valuable reference for further research on the pathogenesis of ulcerous colitis and the development of functional foods to relieve inflammation and balance metabolism.
研究领域:
分析化学
·
医学
·
营养学
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 15
·6669-6677
·
IF 3.553
Li Wan
Hydrogels as drug carriers for delivery of antibiotics are rarely derived from natural molecules and often require complicated synthesis. Puerarin is an herbal natural product that can self-assemble to form pH-sensitive hydrogels without any structural modification. However, Puerarin hydrogels have poor thermostability and weak mechanical strength. In this work, we prepared N -(9-fluorenylmethoxycarbonyl)- l -phenylalanine (Fmoc–Phe–OH) nanofiber-reinforced Puerarin hydrogels with good mechanical and antibacterial properties for suitable drug carriers. The phase transition temperature of Puerarin hydrogels can be increased from 35 to 55 °C by adding only 6 mg/mL of Fmoc–Phe–OH. Rheological study indicated that the presence of Fmoc–Phe–OH not only increased the storage modulus of Puerarin hydrogels by five times, but also did not affect their pH sensitivity. SEM study and pore analysis indicated that Fmoc–Phe–OH nanofibers intertwined with the Puerarin assembly, leading to enhancement of mechanical property of hydrogel. Antibacterial test indicated that the reinforced Puerarin hydrogels have antibacterial property compared with Puerarin hydrogels. Based on these features, a controllable release of loaded model drug (berberine hydrochloride) can be realized from the reinforced Puerarin hydrogels, showing a good synergistic antimicrobial effect on the S. aureus .
研究领域:
药学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）