Chang Wang,Yike Wu,Sheng Liu,Liang Zhang,Bi-Feng Liu,Xin Liu
N-glycans that are fluorescently tagged by glycosylamine acylation have become a promising way for glycan biomarker discovery . Here, we describe a simple and rapid method using Fmoc N-hydroxysuccinimide ester (Fmoc-OSu) to label N-glycans by reacting with their corresponding intermediate glycosylamines produced by microwave-assisted deglycosylation . After optimizing reaction conditions, this derivatization reaction can be effectively achieved under 40 °C for 1 h. Moreover, the comparison of fluorescent intensities for Fmoc-OSu, Fmoc-Cl and 2-AA labeling strategies were also performed. Among which, the fluorescent intensities of Fmoc-OSu labeled glycan derivatives were approximately 5 and 13 times higher than that labeled by Fmoc-Cl and 2-AA respectively. Furthermore, the developed derivatization strategy has also been applied for analyzing serum N-glycans, aiming to screen specific biomarkers for early diagnosis of lung squamous cell cancer. More interestingly, the preparation of free reducing N-glycan standards have been achieved by the combination of HPLC fraction of Fmoc labeled glycan derivatives and Fmoc releasing chemistry . Overall, this proposed method has the potential to be used in functional glycomic study.
Hexavalent chromium (Cr(VI)) pollution is related to human health, and the detection of its concentration has a decisive effect on pollution treatment. In this work, we designed a Cr 2 O 7 2− sensor P(Fmoc-Osu) and applied it in farmland. In the boron trifluoride diethyl etherate solution, the monomer Fmoc-Osu was electropolymerized and obtained its conjugated polymer P(Fmoc-Osu) . The introduction of succinimide and carbonic ester group in side chain enabled it to show high selectivity to Cr 2 O 7 2− in comparison to anions and twenty amino acids. Furthermore, by taking advantage of the signal amplification effect of a conjugated polymer, P(Fmoc-Osu) could analyze Cr 2 O 7 2− in trace amounts and the limit of detection was 8.3 fM. For agricultural application, we employed it in farmland water, soil, Chinese cabbage, carrot and the results showed a satisfactory result. All these results showed that P(Fmoc-Osu) is an efficient tool to detect Cr 2 O 7 2− in practical application.
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