FOOD CHEMISTRY
·
2024
·
IF 8.5
Hao Huang,Shengrong Guo,Yanqun Xu,Fatima-ezzahra Ettoumi,Jie Fang,Xiaowei Yan,Zhangfu Xie,Zisheng Luo,Kejun Cheng
This study introduces an innovative approach for the valorization and protection of anthocyanins from ‘Benihoppe’ strawberry ( Fragaria × ananassa Duch.) based on acidified natural deep eutectic solvent (NADES). Choline chloride-citric acid (ChCl-CA, 1:1) was selected and acidified to enhance the valorization and protection of anthocyanins through hydrogen bond. The optimal conditions (ultrasonic power of 318 W, extraction temperature of 61 °C, liquid-to-solid ratio of 33 mL/g, ultrasonic time of 19 min), yielded the highest anthocyanins of 1428.34 μg CGE/g DW. UPLC-Triple-TOF/MS identified six anthocyanins in acidified ChCl-CA extract. Stability tests indicated that acidified ChCl-CA significantly increased storage stability of anthocyanins in high temperature and light treatments. Molecular dynamics results showed that acidified ChCl-CA system possessed a larger diffusion coefficient (0.05 m 2 /s), hydrogen bond number (145) and hydrogen bond lifetime (4.38 ps) with a reduced intermolecular interaction energy (−1329.74 kcal/mol), thereby efficiently valorizing and protecting anthocyanins from strawberries.
研究领域:
食品科学
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化学
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yicheng Ren,Quan Ma,Dong Li,Yanpei Chen,Qinyang Cheng,Zisheng Luo
Fluctuations in energy status are critical physiological factors influencing postharvest fruit quality. However, the role of autophagy, a stress-resistant biological process, in regulating postharvest fruit energy status remains unclear. In this study, we treated strawberry fruit with the autophagy inhibitor hydroxychloroquine (HCQ) to investigate the role of autophagy in maintaining energy status. The results demonstrated that HCQ treatment inhibited autophagosome formation, reducing relative autophagic activity by 73 % on day 6. HCQ treatment also reduced the energy charge and the NADH/NAD + ratio by 5 % and 61 %, respectively, on day 2. Corresponding to the decline in energy status, HCQ treatment led to a reduction in cytochrome C oxidase (CCO) and succinate dehydrogenase (SDH) activities by 47.48 % and 56.34 %, respectively, on day 3. Additionally, fluctuations in energy charge led to abnormalities in ripening and decreased stress resistance in autophagy-inhibited fruit. In conclusion, this study provides evidence that autophagy-mediated energy charge sustainability mitigates postharvest quality deterioration in fruit.
研究领域:
植物生理学
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采后生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PLANT BIOTECHNOLOGY JOURNAL
·
2019
·Vol. 18
·期 7
·1550-1561
·
IF 6.84
Qi Gao,Huifeng Luo,Yongping Li,Zhongchi Liu,Chunying Kang
Summary Fruit colour affects consumer preference and is an important trait for breeding in strawberry. Previously, we isolated the Reduced Anthocyanins in Petioles ( RAP ) gene encoding a glutathione S-transferase (GST) that binds anthocyanins to facilitate their transport from cytosol to vacuole in the diploid strawberry Fragaria vesca . The parent of rap was the F. vesca variety ‘Yellow Wonder’ that develops white fruit due to a natural mutation in the FveMYB10 gene. Here, we investigated the application potential of RAP in modulating fruit colours by overexpression of RAP in F. vesca and knockout of RAP in the cultivated strawberry Fragaria × ananassa . Unexpectedly, the RAP overexpression in Yellow Wonder background caused formation of red fruit. In addition, the red coloration occurs precociously at floral stage 10 and continues in the receptacle during early fruit development. Transcriptome analysis revealed that the anthocyanin biosynthesis genes were not up-regulated in RAP-ox; rap myb10 flowers at anthesis and largely inhibited at the turning stage in fruit, suggesting a coloration mechanism independent of FveMYB10 . Moreover, we used CRISPR/Cas9 to knockout RAP in cultivated strawberry which is octoploid. Six copies of RAP were simultaneously knocked out in the T0 generation leading to the green stem and white-fruited phenotype. Several T1 progeny have segregated away the CRISPR/Cas9 transgene but maintain the green stem trait. Our results indicate that enhancing the anthocyanin transport could redirect the metabolic flux from proanthocyanidin to anthocyanin production at early developmental stages of fruit and that RAP is one promising candidate gene in fruit colour breeding of strawberry.
研究领域:
植物生物学
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遗传学
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分子生物学
·
农学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PLANT BIOLOGY
·
2022
·Vol. 66
·期 1
·35-46
·
IF 3.15
Dun Wanwan,Wei Xuan,Wang Lu,Liu Jingjing,Zhao Jing,Sun Peipei,Fang Congbing,Xie Xingbin
The strawberry ( Fragaria × ananassa ) is an economically important perennial crop plant, and its fruits are rich in vitamin C ( l -ascorbic acid [AsA]) and other nutrients. l -galactono-1,4-lactone dehydrogenase (GalLDH) is a key enzyme in the terminal step of AsA biosynthesis pathway in plants. Here, the GalLDH gene ( FaGalLDH ) was cloned from ‘Benihoppe’ strawberries. AsA content increased during fruit development and peaked at the red-ripening stage, and AsA concentrations in different tissues were correlated with enzyme activity and transcription level of FaGalLDH . Transient over-expression of FaGalLDH in strawberry fruit increased its overall expression and AsA production significantly, whereas transient RNAi of FaGalLDH decreased its expression and AsA content. Furthermore, the optimum pH and temperature for FaGalLDH activity were 8.0 and 25 °C, respectively. Ectopic expression of the FaGalLDH gene in Arabidops is resulted in higher AsA content and enzyme activity in transgenic lines than in wild-type plants. FaGalLDH over-expression resulted in enhanced tolerance to salt stress due to reduced accumulation of malondialdehyde, H 2 O 2 , and O 2 .− , as well as higher survival rates, root length, proline content, and superoxide dismutase, peroxidase, and catalase activities. These results provide useful information regarding AsA biosynthesis and salt tolerance, which may help to improve strawberry fruit quality and productivity.
研究领域:
植物生理学
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分子生物学
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胁迫耐受性
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生物化学
✓ 已验证
DOI
高影响因子
Food Chemistry: Molecular Sciences
·
2025
·
IF 4.7
Zhenpeng Zhou,Liyuan Zhao,Jingyao Sun,Qingyun Li,Bing-Bing Cai,Xin-Xin Wang
Strawberry ( Fragaria × ananassa Duch . ) is an important economic crop all around the world. A new developed cultivar ‘Baozhu’ which has a similar fruit shape with ‘Hongyan’ is not yet proved to be a different genotype. The main purpose of the present study was to confirm the differences in genotypes and quality and volatile compounds of the two cultivars. The results showed that ‘Baozhu’ cultivar exhibited 39.6 % higher fruit weight, 35.1 % higher total soluble solids, 29.5 % higher firmness, 25.0 % higher total acidity, 45.0 % higher soluble sugars compared to ‘Hongyan’ cultivar ( p < 0.05). A total of 51 volatile compounds were identified in the strawberries using headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS), and esters were the most abundant compounds, followed by aldehydes and alcohols. The results of chemometrics analysis reveal that ‘Baozhu’ and ‘Hongyan’ strawberries can be distinctly separated from each other. A panel of 28 volatiles with VIP > 1 and p < 0.05 were identified as discriminant factors for ‘Baozhu’ and ‘Hongyan’. Furthermore, ‘Baozhu’ cultivar had significantly more stomata than the ‘Hongyan’ cultivar. A clear and positive correlation was observed among quality, volatile and stomata number. The current findings contribute to the commercial value assessment of the ‘Baozhu’ cultivar at an industrial level, formulating a paradigm for improvement of daily eating pattern and driving the development of genetic tools for improvement of flavor.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PLANT GROWTH REGULATION
·
2025
·
IF 4.4
Zheng Ying,Han Xiaohuan,Zhang Yang,Qiu Weiwen,Tao Tao,Xu Yuting,Li Mohan,Xie Xingbin,Sun Peipei,Zheng Guanghui,Fang Congbing,Zhao Jing
Strawberry fruits are among the popular berries due to their bright colour and desirable flavour. However, their rapid quality loss results in a short shelf life and low economic value. This study aimed to comprehensively examine the effects of preharvest spraying at small green (SG), large green (LG), white (W), and red-turning (RT) fruit stages, along with postharvest treatments using γ -aminobutyric acid (GABA) and its synthesis inhibitor, 3-mercaptopropionic acid (3-MP), on fruit quality and shelf life. Preharvest application of 10 mM GABA at RT stage significantly enhanced the fruit colour, weight, firmness, soluble solids content (SSC), and the contents of GABA, citric acid, Vc, and soluble sugars at harvest. Postharvest treatment with 10 mM GABA notably delayed the decreases in organic acids, soluble sugars, firmness, colour, and SSC. In addition, postharvest 10 mM GABA treatment reduced the decay incidence and weight loss, while 0.2 mM 3-MP had opposite effects in both preharvest and postharvest treatments. GABA partially induced or inhibited the expression of genes involved in citrate metabolism. Overall, GABA demonstrates effectiveness in improving fruit quality (e.g., increase in organic acids, soluble sugars, firmness, colour, and SSC after preharvest treatment or delaying the decrease of these indices) and extending shelf life by modulating the transcripts of genes related to citrate metabolism.
研究领域:
植物学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 102
·期 11
·4589-4598
·
IF 4.125
Wenshuo Zhang,Xiaojie Liu,Shibo Qiu,Lin Chen,Xinghai Liu
BACKGROUND A shelf-life that is too short is the main problem with strawberries. Nano-TiO 2 can catalyze and oxidize ethylene under ultraviolet (UV) light irradiation to eliminate its ripening effect on fruits and prolong the freshness period. RESULTS In this work, nano-TiO 2 modified by methacryloxy propyl trimethoxyl silane (KH570) was blended with low-density polyethylene (LDPE) to prepare modified atmosphere packaging (MAP), and the influence of TiO 2 content on films was analyzed before and after UV treatment. The results show that the modified nano-TiO 2 (M-TiO 2 ) can be uniformly distributed in LDPE, improving its mechanical strength, hydrophobicity, oxygen barrier and UV shielding properties. A modified atmosphere with low ethylene, low O 2 and high CO 2 can be created to inhibit the ripening and spoilage of strawberries. The weight loss rate of fruit can be effectively reduced. The tendency of fruit firmness decline and nutrient loss can be slowed and stabilized, contributing to controllable shelf-life. Excellent freshness preservation function can be realized without special UV treatment. CONCLUSION Since UV treatment is rare in actual storage and transportation, LDPE/M-TiO 2 composite film has practical value as MAP for strawberry and similar non-climacteric fruits. © 2022 Society of Chemical Industry.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACTA PHYSIOLOGIAE PLANTARUM
·
2014
·Vol. 36
·期 9
·2441-2451
·
IF 1.524
Chen Jingxin,Mao Linchun,Mi Hongbo,Zhao Yuying,Ying Tiejin,Luo Zisheng
To study the detachment stress on the ripeness of strawberry fruit, physiological characteristics of strawberry fruit on and off plant during ripeness and senescence processes were investigated. The results indicated that the ripeness of strawberry fruit upon detachment was accelerated, in terms of firmness, soluble solid content and especially color development. The color of fruit off plant changed rapidly from white to full red in 1–2 days. The respiratory rate in fruit off plant was strengthened, higher than that on plant. Abscisic acid level and ethylene production in fruit off plant were also higher than those on plant and auxin degradation was exacerbated by detachment. Expression levels of FaMYB1 , FabHLH3 and FaTTG1 were generally reduced with phenotypes of redder color and more anthocyanin accumulation in fruit off plant. Results also suggested that the detachment initially stimulated ethylene and abscisic acid production and auxin degradation, which modulated ripening-related gene expression and at last enhanced fruit pigmentation.
研究领域:
植物学
·
生理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）