The tubers of Bletilla striata were fermented with Fusarium avenaceum and Fusarium oxysporum . The total phenolic contents (TPCs) and antioxidant and antibacterial activities of the extracts from the fermented products were measured. Additionally, the extracts of both F. avenaceum and F. oxysporum fermented B. striata (FBS) were characterized by UV–vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. The results showed that the F. avenaceum and F. oxysporum FBSs possessed higher TPCs and exhibited stronger antioxidant and antibacterial activities ( Bacillus subtilis ) in comparison with original material not co-fermented with microorganisms. Chemical characterization of the two extracts with UV–vis, FT-IR, and 1 H NMR implied that some of the phenolic glycosides may be deglycosylated and that bibenzyl compounds and saccharides may be metabolized in both FBS systems. Microbial fermentation enhances the TPC as well as the antioxidant and antibacterial activities of B. striata . The present study suggests that F. avenaceum and F. oxysporum fermentations were effective in B. striata processing.
Jingyi Tang,Haibin Liao,Xueqin Fu,Yonghong Xie,Zhengrong Zou,Zaizhi Liu
Methyleugenol was encapsulated via ultrasonic assisted microencapsulation method. • High yield and encapsulation efficiency were obtained with the proposed method. • Methyleugenol microcapsules had good thermal stability and sustained-release ability. • Methyleugenol microcapsules showed remarkable antifungal activity on F. avenaceum . Methyleugenol microcapsules were successfully fabricated using soybean protein isolate and sodium alginate as wall materials through ultrasonic assisted microencapsulation method. A real yield of 95.45 ± 2.55% and an encapsulation efficiency of 76.95 ± 3.03% were achieved under the optimum conditions derived from Plackett–Burman and Box–Behnken designs. Physicochemical characterization confirmed effective methyleugenol encapsulation, with the microcapsules exhibiting irregular morphology, uniform particle size distribution, and distinctive wrinkled surface textures. Thermogravimetric curve indicated that the thermal stability of methyleugenol was obviously enhanced by the microencapsulation process. After microencapsulation, the antifungal activity of methyleugenol was greatly improved, with its microcapsules exhibiting comparable efficacy to nystatin against Fusarium avenaceum , demonstrating the minimum inhibitory concentration (MIC) of 0.10 mg/mL and 0.07 mg/mL, respectively. These findings provide a scientific basis for exploring the potential applications of ME microcapsules in food preservation and related industrial sectors.
危险品化学品经营许可证（带存储）