PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2021
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IF 3.963
Yanan Sun,Haiping Shi,Chenxin Mao,Jianyan Wu,Chuanqing Zhang
The occurrence of bakanae disease of rice caused by the fungus Fusarium fujikuroi in Zhejiang Province has become increasingly aggravated in recent years, concomitant with the development of resistance to the widely applied fungicides , prochloraz and phenamacril. In this study, the activity of a novel succinate dehydrogenase inhibitor (SDHI) fungicide, penflufen, against different fungi was evaluated in addition to the potential of penflufen in controlling F. fujikuroi infections. Penflufen exhibited good bioactivity against F. fujikuroi , but weak activity against Fusarium spp. and other investigated plant-pathogenic fungi including Colletotrichum spp. In addition to inhibiting mycelial growth , penflufen effectively inhibited F. fujikuroi conidium production. For germination, penflufen could effectively inhibit that of small conidia, but only delay the germination of large conidia. In addition, the sensitivity to penflufen among 100 F. fujikuroi isolates that were collected in areas that were never exposed to SDHIs was determined based on mycelium growth. Sensitivities surprisingly exhibited bimodal distributions, indicating the presence of natural resistance. Cross-resistance was not observed between penflufen in F. fujikuroi and two fungicides that have been extensively applied in field including prochloraz (a DMI) and phenamacril (a 2-cyanoacrylate fungicide), nor with the three SDHIs, fluopyram, benzovindiflupyr, and pydiflumetofen. Additional analysis identified five different point mutations in SDH-A (i.e., at residues 46, 225, 283, 430, and 586) of naturally resistant isolates. These results inform the potential application of the new SDHI fungicide penflufen for managing crop diseases and understanding possible resistance mechanisms among pathogens .
研究领域:
植物病理学
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真菌学
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农药科学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 12
·6658
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IF 6.208
Kehan Zhao,Lianmeng Liu,Shiwen Huang
Fungal basic leucine zipper (bZIP) proteins play a vital role in biological processes such as growth, biotic/abiotic stress responses, nutrient utilization, and invasion. In this study, genome-wide identification of bZIP genes in the fungusFusarium fujikuroi, the pathogen of bakanae disease, was carried out. Forty-four genes encoding bZIP transcription factors (TFs) from the genome ofF. fujikuroi(FfbZIP) were identified and functionally characterized. Structures, domains, and phylogenetic relationships of the sequences were analyzed by bioinformatic approaches. Based on the phylogenetic relationships with the FfbZIP proteins of eight other fungi, the bZIP genes can be divided into six groups (A–F). The additional conserved motifs have been identified and their possible functions were predicted. To analyze functions of the bZIP genes, 11FfbZIPswere selected according to different motifs they contained and were knocked out by genetic recombination. Results of the characteristic studies revealed that theseFfbZIPs were involved in oxygen stress, osmotic stress, cell wall selection pressure, cellulose utilization, cell wall penetration, and pathogenicity. In conclusion, this study enhanced understandings of the evolution and regulatory mechanism of theFfbZIPsin fungal growth, abiotic/biotic stress resistance, and pathogenicity, which could be the reference for other fungal bZIP studies.Keywords:bZIP TF;bakanae disease;Fusarium fujikuroi;phylogenetic analysis;functional analysis
研究领域:
真菌学
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植物病理学
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基因组学
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生物信息学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2024
·
IF 9.7
Tian-Qiong Shi,Yi-hang Shen,Ya-Wen Li,Zi-Yi Huang,Zhi-Kui Nie,Chao Ye,Yue-Tong Wang,Qi Guo
Gibberellic acid (GA3), produced industrially by Fusarium fujikuroi , stands as a crucial plant growth regulator extensively employed in the agriculture filed while limited understanding of the global metabolic network hinders researchers from conducting rapid targeted modifications. In this study, a small-molecule compounds-based targeting technology was developed to increase GA3 production. Firstly, various small molecules were used to target key nodes of different pathways and the result displayed that supplement of terbinafine improved significantly GA3 accumulation, which reached to 1.08 g/L. Subsequently, lipid and squalene biosynthesis pathway were identified as the key pathways influencing GA3 biosynthesis by transcriptomic analysis. Thus, the strategies including in vivo metabolic engineering modification and in vitro supplementation of lipid substrates were adopted, both contributed to an enhanced GA3 yield. Finally, the engineered strain demonstrated the ability to achieve a GA3 yield of 3.24 g/L in 5 L bioreactor when utilizing WCO as carbon source and feed.
研究领域:
植物科学
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分子生物学
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生物技术
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代谢工程
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DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGICAL METHODS
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2018
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IF 1.701
Yu-Ke Cen,Jian-Guang Lin,Jun-You Wang,Zhi-Qiang Liu,Yu-Guo Zheng
A colorimetric assay has been developed for quantitative analysis of active biomass of Fusarium fujikuroi , based on the reduction of the tetrazolium salt 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) when menadione was present as an electron coupling agent. The optimum assay-conditions were set as 200 μg/ml XTT, 5 μM menadione and one-hour reaction time. Under these settings, the produced formazan displayed a linear relationship with F. fujikuroi biomass. This method was subsequently applied to evaluate the cell growth behavior, which showed a positive correlation with the carbon source consumption and gibberellin biosynthesis under the industrial fermentation conditions. Our results showed that the XTT-menadione assay is a valuable tool in analyzing the industrial fermentation process of F. fujikuroi , especially when the medium contains insoluble and complex components.
研究领域:
微生物学
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生物技术
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真菌生物学
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DOI
高影响因子
3 Biotech
·
2020
·Vol. 10
·期 7
·1-10
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IF 1.798
Peng Xiao-Lun,Zhao Wei-Jun,Wang Yuan-Shan,Dai Ke-Lei,Cen Yu-Ke,Liu Zhi-Qiang,Zheng Yu-Guo
Gibberellic acid (GA 3 ) is a natural plant growth hormone that has been widely used in agriculture and horticulture. To obtain higher GA 3 producing strains, the method of screening the strains for resistance to simvastatin was used after treatment with nitrosoguanidine (NTG) and gamma rays. The rationale for the strategy was that mutants showing simvastatin resistance were likely to be high GA 3 producers, as their activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is relatively more effective. GA 3 yield of mutant S109 increased by 14.2% than that of the original strain. The GA 3 production ability in mutant S109 remained relatively stable after ten generations. With the addition of 0.4 g glycerol on the 5th day during the fermentation process in Erlenmeyer flask, maximum GA 3 production of 2.7 g/L was achieved by this mutant, exhibiting 28.6% increase compared with original strain. Furthermore, we also achieved 2.8 g/L GA 3 and had a 33.3% increase with addition 20 g glycerol on the 5th day during the fermentation process in a 5-L bioreactor. Our results indicated efficient GA 3 production could be achieved on the condition that the supply of glycerol at the suitable conditions. This study would lay a foundation for industrial production of GA 3 .
研究领域:
植物学
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微生物学
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遗传学
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DOI
PubMed
高影响因子
TRENDS IN BIOTECHNOLOGY
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2026
·
IF 16.6
Ya-Wen Li,Xin-Yu Chen,Hui-Zhi Lu,Ji-Bao Yao,Zhi-Kui Nie,Tian-Qiong Shi
Gibberellic acid (GA3) is primarily produced by the liquid fermentation of Fusarium fujikuroi , yet its complex metabolic network limits rational strain engineering. Here, we strengthened the nonhomologous end joining (NHEJ) pathway and mapped the distribution of integration to establish a genome-scale insertional mutagenesis library in F. fujikuroi . The library enabled rapid identification of ten novel genomic loci that support stable heterologous gene expression, thereby expanding the available genetic toolkit. We further applied the library to uncover functional determinants of GA3 biosynthesis and acetic acid tolerance, identifying previously uncharacterized gene targets. Reverse engineering of these targets yielded a GA3-overproducing strain capable of tolerating and utilizing acetic acid wastewater. In a 5-l bioreactor, the engineered strain NH4 reached a GA3 titer of 3.32 g/l, 3.05-fold higher than the control strain. This study establishes an efficient NHEJ-mediated genome-scale mutagenesis platform for F. fujikuroi and provides more genetic targets for constructing high-yield GA3 cell factories.
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高影响因子
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