EUROPEAN JOURNAL OF PHARMACOLOGY
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2024
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IF 4.2
Songxia Cheng,Jiali Ding,Biao Xu,Yan Wang,Xiaoyu Shen,Yanhua Xia,Lei Wu,Jie Wei
Burn induced-pain (BIP) is one of the most common pain symptoms, which seriously affects the quality of sufferer life. Researches show that multi-targeted drug therapies offer superior efficacy and fewer side effects compared to single-target drug therapies. Consequently, in this study, we developed G(1-5)-EM2, a multi-targeted peptide designed to target μ-opioid receptor and the growth hormone secretagogue receptor 1α (GHS-R1α), and explored its antinociceptive effects on burn injury pain. Calcium mobilization experiments revealed that G(1-5)-EM2 demonstrated weak multi-agonist activities to μ-opioid receptor and κ-opioid receptor as well as GHS-R1α in vitro. Near-infrared fluorescence imaging experiments demonstrated that G(1-5)-EM2 could penetrate the blood-brain barrier (BBB) and access the brain following intravenous injection. The enzymatic stability of G(1-5)-EM2 was significantly enhanced compared to EM2. Our results indicated that intrathecal administration of G(1-5)-EM2 mitigated mechanical allodynia and thermal hyperalgesia in BIP. These antinociceptive effects of G(1-5)-EM2 were partially mediated through μ-opioid receptor and GHS-R1α. Moreover, intrathecal administration of G(1-5)-EM2 significantly decreased burn-induced up-regulation of phosphorylated p38 MAPK, phosphorylated NF-κBp65 and TRPV1 in the ipsilateral spinal cord, reduced the levels of IL-1β, IL-6 and TNF-α in serum, and enhanced wound healing in burned skin. Repeated intrathecal administration of G(1-5)-EM2 produced a non-tolerance-forming antinociception in BIP. These results suggest that the multi-targeted peptide G(1-5)-EM2 exhibits a novel role in alleviating BIP with fewer side effects and may represent a promising strategy for developing new analgesic drugs.
研究领域:
药理学
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疼痛研究
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
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2016
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IF 4.803
Pengfei Wang,Hui Liu,Yuxing Xu,Yunfa Chen,Jun Yang,Qiangqiang Tan
Reducing the particle size is a straightforward way to increase the specific surface area of ruthenium oxide, which usually translates to the high specific capacitance for a supercapacitor. Herein, we report a facile strategy to fabricate ultrafine ruthenium oxides supported on various carbon-based substrates (carbon powders, carbon nanotubes, or reduced graphene oxides) as excellent electrode materials for a supercapacitor. The novelty of this work lies in its synthetic approach, which involves an aqueous synthesis of ruthenium nanoclusters under the control of pH value, and an air oxidation-based conversion process. In particular, owing to their ultrafine particle size, the as-prepared carbon-, carbon nanotube-, or reduced graphene oxide-supported ruthenium oxides exhibit specific capacitance as high as 879.1 F g −1 , 966.8 F g −1 and 1099.6 F g −1 , respectively, for a supercapacitor at a current density of 0.5 A g −1 . The specific capacitance maintains 98.4% (for carbon supports), 98.0% (for carbon nanotube supports) and 98.4% (for reduced graphene oxide supports) at current density of 1 A g −1 with good cycling stability. The remarkable simplicity and environmental friendliness of this synthesis may provide a liable quantity production route to produce ruthenium oxides as highly efficient electrode materials for a supercapacitor.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
Autophagy
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2018
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IF 11.1
Zhen Yang,Qi Sun,Junfei Guo,Shixing Wang,Ge Song,Weiying Liu,Min Liu,Hua Tang
Emerging evidence has revealed that miRNAs could upregulate the expression levels of target genes. However, the molecular mechanism underlying upregulation of targets mediated by miRNAs remains unclear. In this study, we found a novel miRNA named MIR-G-1 by GRSF1-RNA immunoprecipitation (RIP)-deep sequencing, which could directly target and upregulate LMNB1 and TMED5 in a GRSF1-dependent manner in cervical cancer cells. In addition, upregulated MIR-G-1 in cervical cancer promoted a malignant phenotype in vitro and in vivo. TMED5 could interact with WNT7B and thus activated the canonical WNT-CTNNB1/β-catenin signaling pathway. MIR-G-1 mediated the activation of this pathway. Furthermore, MIR-G-1 promoted serum starvation-induced nuclear macroautophagy/autophagy, and accelerated taxol (TAX)-induced DNA-damage repair in cervical cancer cells. Collectively, these findings may provide a new insight into the upregulation mechanism and nuclear autophagy mediated by miRNAs and provide a potential biomarker for cervical cancer.Abbreviations: 3ʹUTR: 3′ untranslated region; EMSA: electrophoretic mobility shift assay; EMT: epithelial-mesenchymal transition; GRSF1: G-rich RNA sequence binding factor 1; IF: immunofluorescence; IP: immunoprecipitation; IHC: immunohistochemistry; lnc: long noncoding; miRNA:microRNA; TAX: taxol; TMED5: transmembrane p24 trafficking protein 5
研究领域:
肿瘤学
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分子生物学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2019
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IF 14.229
Ge Shu,Yuda Li,Zhuang Wang,Jia-Xing Jiang,Feng Wang
Conjugated polymers have recently emerged as encouraging metal-free photocatalysts for solar energy conversion under visible light irradiation. Unfortunately, photocatalytic activities of the current polymers are still inadequate. Herein, we present a simple but quite attractive conjugated homopolymer (PDBTSO) based a dibenzothiophene-S,S-dioxide moiety. The introduction of sulphonyl group to a polyfluorene-like backbone can cause richly available active sites and lead to the efficient separation of photoexcited charge carriers, thus yielding impressive photocatalytic performance. Furthermore, we explored a natural “phosphorylation” strategy for increasing photocatalytic hydrogen production over PDBTSO. Remarkably, an extremely high hydrogen evolution rate of 44.2 mmol h −1 g −1 was achieved with the optimum addition of K 2 HPO 4 under visible light irradiation.
研究领域:
材料科学
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能源化学
✓ 已验证
DOI
高影响因子
JOURNAL OF POWER SOURCES
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2019
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IF 7.467
Zeyi Yao,Wu Tang,Xinxin Wang,Chuan Wang,Chuluo Yang,Cong Fan
The organic cathodes with high specific capacity are highly desirable to fabricate high-specific-energy-density organic Li-ion full cells. Herein, we initially propose the long-term plan for the systematic synthesis of the oligomer family of 1,4-benzoquinone (BQ), which can possess theoretical specific capacity above 500 mA h g −1 . As a start, the dimer of 1,4-benzoquinone (2,2′-bis-(1,4-benzoquinone), BBQ) is synthesized, which can realize the specific capacity of 501 mA h g −1 and the average discharge potential of ~2.5 V (vs. Li + /Li). Even facing the dissolution problem, BBQ can deliver average specific capacities of ~190 mA h g −1 during 3000 cycles at 500 mA g −1 and ~120 mA h g −1 during 8000 cycles at 2 A g −1 in Li-ion half cells. Afterwards, the all-organic Li-ion full cells are fabricated by using the reduced state (Li 4 TP) of lithium terephthalate (Li 2 TP) as the starting anode and BBQ as the starting cathode. Delightfully, the resulting Li 4 TP-BBQ Li-ion full cells can realize the specific capacity of 316 mA h g −1 cathode in the 1st cycle (500 mA g −1 ). Meanwhile, average specific capacities of ~98 mA h g −1 during 1000 cycles can be fulfilled (500 mA g −1 ). The results of the Li 4 TP-BBQ Li-ion full cells are among the best all-organic Li-ion full batteries reported to date.
研究领域:
材料科学
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电化学
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能源存储
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Muguli Muhaxi,Fang Liu,Tzi Bun Ng
In the present study, three novel antioxidant polysaccharides (G-1, AG-1, and AG-2) were isolated and purified from Pleurotus ferulae using mouse erythrocyte hemolysis inhibitory activity as an indicator. These components showed antioxidant activity at the chemical and cellular levels. Given that G-1 displayed superior performance in protecting the human hepatocyte L02 cells against oxidative damage caused by H 2 O 2 compared to AG-1 and AG-2 and had a higher yield and purification rate, the detailed structure of G-1 was further characterized. G-1 mainly contains six kinds of linkage type units as A: →4,6)-α- d -Glcp-(1→, B: →3)-β- d -Glcp-(1→, C: →2,6)-β- d -Glcp-(1→, d : β- d -Manp(1→, E: →6)-β- d -Galp-(1→, F: →4)-β- d -Glcp-(1→. Finally, the potential in vitro hepatoprotective mechanism of G-1 was discussed and elucidated. Results suggested that G-1 can protect L02 cells from H 2 O 2 -induced damage by reducing the leakage of AST and ALT from the cytoplasm, enhancing the activities of SOD and CAT, and suppressing lipid peroxidation and production of LDH. G-1 could further reduce the production of ROS, stabilize mitochondrial membrane potential and maintain cell morphology. Hence, G-1 could be a valuable functional food with antioxidant and hepatoprotective activities.
研究领域:
真菌学
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药理学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）