Photonics
·
2024
·Vol. 11
·期 8
·708
·
IF 2.1
Mingzhong Zhang,Shanshan Xu,Peng-Cheng Guan,Yue-Jiao Zhang,Jian-Feng Li
In surface-enhanced Raman scattering (SERS) detection research, the shape, structure, surface modification, and material selection of nanoparticles can significantly impact the SERS intensity. Petal-like gap-enhanced Raman tags (P-GERTs) possess numerous sharp tips and edges, which generate localized electric field enhancements, further amplifying the electric field enhancement effect on neighboring molecules and enhancing the SERS signal. Additionally, the surface of P-GERTs can be modified with functional molecules, enabling their application in the detection of disease biomarkers. Using COVID-19 as an example, the performance of P-GERTs in disease biomarker detection was validated, demonstrating that the signal intensity of this probe can reach 55 times that of regular gold nanoparticles and 36.7 times that of smooth shell gap-enhanced Raman tags (S-GERTs). Furthermore, in combination with magnetically retrievable magnetic bead substrates, the N-protein antigen was specifically detected in a one-step process. N-protein was detected within 15 min using a portable Raman spectrometer. The limit of detection (LOD) for the standard sample was 4.28 pg/mL, and the LOD for the actual throat swab sample system was 25.4 pg/mL. This workflow can be extended to the detection of other biomarkers, making it widely applicable.
研究领域:
分析化学
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生物医学工程
·
纳米技术
·
分子生物学
·
诊断医学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Jiajia Shan,Xinjing Li,Shuqing Han,Tao Ren,Mengke Jin,Xue Wang
Surface Enhanced Raman Spectroscopy (SERS) is a highly sensitive detection method, however, it is still challenging to directly detect florfenicol due to its low Raman activity. To solve this problem, we present a novel sensor for selective detection of florfenicol residue in milk using gap-enhance Raman tags (GERTs) competitive immunoassay. In this nanosensor, Raman reporters 4-mercaptobenzoic acid (4MBA) were embedded in the 1 nm gap of Au core-gap-Au shell nanoparticles, which can protect 4MBA molecules from unwanted external environments and provide a high enhancement. The parameters affecting the detection sensitivity were discussed and optimized. The limit detection of florfenicol in milk can reach 0.1 μg/kg. To evaluate the performance of GERTs immunoassay, [email protected] tags immunoassay was conducted and the results were compared. Moreover, Raman mapping was investigated to improve the quantitative prediction model with R 2 of 0.981 achieved, by eliminating the signal fluctuations caused by the inhomogeneous distribution of GERTs.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
光谱学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2021
·Vol. 9
·期 2
·2102405
·
IF 16.806
Binge Deng,Yaohui Wang,Yifan Wu,Wenjin Yin,Jinsong Lu,Jian Ye
The accurate positioning of sentinel lymph node (SLN) by tracers during surgery is an important prerequisite for SLN biopsy. A major problem of traditional tracers in SLN biopsy is the short surgery window due to the fast diffusion of tracers through the lymphatics, resulting in a misjudgment between SLN and second echelon lymph node (2 nd LN). Here, a nontoxic Raman nanoparticle tracer, termed gap-enhanced Raman tags (GERTs), for the accurate intraoperative positioning of SLNs with a sufficient surgical time window is designed. In white New Zealand rabbit models, GERTs enable precise identification of SLNs within 10 min, as well as provide the surgeon with a more than 4 h time window to differentiate SLN and 2 nd LN. In addition, the ultrahigh sensitivity of GERTs (detection limit is 0.5 × 10 −12 m ) allows detection of labeled SLNs before surgery, thereby providing preoperative positioning information for minimally invasive surgery. Comprehensive biosafety evaluations carried out in the context of the Food and Drug Administration and International Standard Organization demonstrate no significant toxicity of GERTs, which supports a promising clinical translation opportunity of GERTs for precise SLN identification in breast cancer.
研究领域:
肿瘤学
·
生物医学工程
·
外科手术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2021
·
IF 12.479
Zhouzhou Bao,Binge Deng,Yuqing Zhang,Xiaowei Li,Ziyang Tan,Zhuowei Gu,Bobo Gu,Zhifeng Shao,Wen Di,Jian Ye
Sentinel lymph node (SLN) imaging and biopsy has been advocated as an important technique to evaluate the metastatic status of regional lymph nodes and determine subsequent surgical procedure for many cancers, yet there is no reliable means to provide accurate and rapid diagnosis of metastatic SLN during surgery. Here we develop a new approach, named “Ratiometric Raman dual-nanotag strategy”, that using folic acid functionalized targeted and nontargeted gap-enhanced Raman tags (FA-GERTs and Nt-GERTs) to detect metastatic SLN based on Raman imaging combined with classical least square data processing methods. By using this strategy, with built-in self-calibration for signal correction, rather than absolute intensity-dependent signal readout, we realize the visualization and prompt intraoperative diagnosis of metastatic SLN with a high accuracy of 87.5 % when the cut-off value of ratio (FA-GERTs/Nt-GERTs) set at 1.255. This approach may outperform the existing histopathological assessment in diagnosing SLN metastasis and is promising for guiding surgical procedure in the future.
研究领域:
癌症生物学
·
生物医学工程
·
外科肿瘤学
·
分子成像
·
诊断技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）