Xiaomeng Li,Beibei Hu,Lijie Ma,Yuechan Shi,Yongshuai Jing,Zhongqiu Li,Zhiwei Li,Shiguo Sun
Growth hormone-releasing hexapeptide (GHRP-6) is a kind of stimulant, which can be illegally used in athletic performance enhancement during sports. The accurate and sensitive detection of GHRP-6 is crucial for ensuring fair competition. Electrochemical sensors achieve quantitative analysis by monitoring the changes in electrochemical signals induced by target analytes. In this study, we developed an electrochemical sensor based on voltammetry to detect GHRP-6, providing a novel and advanced analytical methodology for its detection. The GHRP-6 was used as templates, phenol as polymer monomers, and MWCNTs as signal amplification materials, to build a detection method based on molecularly imprinted electrochemistry (MIEC). The fabricated sensor displayed rapid binding kinetics due to the location of all imprinting cavities on the polymer’s surface. This method displayed a linear relationship in the concentration range of 0.1 ∼ 100 ng/mL, featuring a detection limit of 1.21 pg/mL (S/N = 3). The percent recovery of GHRP-6 was 96.0 ∼ 103.2 % with the RSD in the range of 1.49 ∼ 5.56 %. The reproducibility test of the method resulted in an RSD of 3.19 %. Compared to the normal detection method, this technique shows more reproducibility and stability in real human serum samples. This study introduces an advanced, simple, and practical detection method for GHRP-6 in clinical samples.
Yumeng Cao,Xin Gao,Yue Zhan,Mengtao Duan,Molin Yang,Beibei Hu,Zhongqiu Li,Zhiwei Li
Growth hormone releasing peptide-6 (GHRP-6), a polypeptide with potent growth hormone secretagogue activity, has wide-ranging applications in medical research and sports science. However, the escalating issues of illegal administration and abuse highlight an urgent need for highly efficient and sensitive detection methodologies. To address the challenge of trace detection of GHRP-6, this study constructs a ratiometric electrochemical sensor, MIP/[APMIm]Br/CS@MWCNTs/GCE, by integrating molecularly imprinted technology (MIT) with electrochemical sensing technology. Thionine (Thi) as an internal reference signal and [APMIm]Br ionic liquid as a functional modifier to establish a dual-signal transduction mechanism. By using the dual-signal ratio (I Fe /I Thi ) to eliminate matrix environmental interference, the sensor achieves a detection limit as low as 1.0 pg/mL, over a linear range of 0.3–80 ng/mL. Notably, it demonstrates the recoveries of 98.1%–104.6% in the presence of 100-fold concentration interferents, with a repeatability RSD of 2.04% and only 6.4% signal degradation after 30 days. These performance parameters represent a significant advancement over conventional detection techniques, establishing a robust platform for trace-level GHRP-6 analysis.
危险品化学品经营许可证（带存储）