Headspace separation combined fluorescence detection of hydrogen sulfide with silver-sensitized lanthanide coordination polymer via a simple self-made device
Hydrogen sulfide (H 2 S), playing unique role on signal transmission in various physiological processes, the rapid and accurate monitoring of it is particularly concerned. The drawbacks of fluorescence probe for H 2 S, namely poor selectivity or long response time, have always existed. As a test case, here headspace separation combined fluorescence strategy was attempted, on basis of H 2 S stimuli-responsive AMP/Tb 3+ /Ag + or GMP/Tb 3+ /Ag + coordination polymer probes. The fluorescence intensity of them is inversely proportional to the concentration of it, due to Ag-S in situ coordination and partial formation of Ag 2 S. Using the hybrid analysis, a quick response of less than 2.0 min and a high selectivity could be obtained. The signal change (Δ I ) of AMP/Tb 3+ /Ag + or GMP/Tb 3+ /Ag + showed good linearity within the range of 0.5–100 μM and 1.0–150 μM, along with a detection limit (3 σ ) of 0.2 and 0.4 μM, respectively. When utilized to determine the sulfide level in real samples, it shows recoveries from 96.4 % to 102.3 % with relative standard derivation ( RSD ) less than 3.0 %. The combination strategy of headspace separation and fluorescence quantitative analysis was verified to effectively eliminate potential interference such as other coexisting non-volatile thiol-containing species, providing a novel avenue to flexibly design the volatile component analysis with high selectivity in complex sample matrixes.
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