COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Jingjing Xu,Jingyi Wei,Yanying Cui,Sixian Yu,Sufang Guo,Qi An,Ping Han
In this work, an Au/g-C 3 N 4 /GO/Au hybrid nanofilm for surface-enhanced Raman scattering (SERS) substrate application was prepared by a facile layer-by-layer assembly technique using nanosheet-structured graphitic carbon nitride (g-C 3 N 4 ), graphene oxide (GO) and 40 nm-gold nanoparticles (Au NPs). The effects of composition, assembly sequence and assembly cycles on the SERS performance were optimized to achieve the SERS-active substrate. The detection limit of Au/g-C 3 N 4 /GO/Au substrate for crystal violet and rhodamine 6 G probe molecules reached as high as 10 -8 M, with good stability and uniformity. Its further application in food colorants detection (erythrosine, carmine and temptation red) had successfully met the national food safety standards and the quantitative detection for erythrosine and temptation red had also been established. Based on finite-difference time-domain element simulation and density functional theory quantum chemical calculation, the mechanism of the SERS effect of the substrate was unveiled, which is attributed to the combination of electromagnetic enhancement effect of Au nanoparticle aggregates and chemical enhancement effect of GO and g-C 3 N 4 . Notably, water droplet evaporation induced Raman enhancement was observed on the Au/g-C 3 N 4 /GO/Au substrate. This can be ascribed to the inhomogeneous evaporation of the droplet that promotes the enrichment of the probe molecules into the center of the droplet. This novel enhancement strategy provides a new idea for further improving detection sensitivity of SERS substrate materials.
研究领域:
材料科学
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分析化学
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表面科学
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纳米技术
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DOI
高影响因子
Polymers
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2025
·Vol. 17
·期 12
·1716
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IF 4.9
Lili Kong,Xinna Yu,Qifang Sun,Meizhen Huang,Tianyuan Liu,Jie Chen
Polycyclic aromatic hydrocarbons (PAHs) have attracted significant attention due to their severe threats to both ecological systems and human health. In this paper, a high-performance surface-enhanced Raman spectroscopy (SERS) substrate based on NCF/GO/Au@Ag nanocomposites was developed, which enabled sensitive and stable detection of PAHs. The NCF/GO/Au@Ag substrate synergistic utilizes the localized surface plasmon resonance (LSPR) effect of Au@Ag core–shell nanorods and the additional interfacial charge transfer provided by graphene oxide (GO) to exhibit extremely high sensitivity. And the three-dimensional fibrous network of nanocellulose (NCF) improved nanoparticle dispersion uniformity. Combined finite element simulations and experimental studies verified that the dual plasmonic resonances (512 nm and 772 nm) of Au@Ag nanorods optimally match 785 nm excitation, yielding an enhancement factor of 5.21 × 10 5 . GO integration enhanced Raman signals by 1.68-fold through interfacial charge transfer, while the introduction of NCF reduced the signal relative standard deviation (RSD) from 36.88% to 4.29%. The NCF/GO/Au@Ag substrate achieved a detection limit of 10 μg/L for PAHs, demonstrating exceptional sensitivity and reproducibility.
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2024
·Vol. 31
·期 32
·44759-44769
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IF 0
Wu Rui,Song Xi,Tian Guanghui
Highly active Fe 3 O 4 /GO/Au composite nanomaterial was fabricated as a substrate of surface-enhanced Raman spectroscopy (SERS) and applied for pesticide residue detection. The three-layer multifunctional Fe 3 O 4 /GO/Au nanoparticles (NPs) were designed by facile method, with high hotspots, and were characterized by various techniques, including ultraviolet spectrophotometry (UV), X-ray diffraction (XRD), infrared absorption spectrometer (IR), and transmission electron microscopy (TEM). The performance of Fe 3 O 4 /GO/Au was evaluated by Raman spectroscopy with R6G as a probe molecule to verify its enhancement effect. It exhibited a strong Raman signal with 10 −6 M of R6G. Furthermore, the presence of Fe 3 O 4 /GO/Au nanohybrid enabled the SERS-based method to detect mancozeb and showed an excellent linear relationship in the range of 0.25–25 ppm, with a low limit of detection (0.077 ppm), satisfactory EF, stability, and repeatability. In addition, the mechanism of SERS enhancement with electromagnetic mechanism (EM) and chemical mechanism (CM) was discussed in detail. Therefore, the proposed SERS approach holds promise as an auxiliary technique for screening contaminated agricultural products, environmental sample, and food in the future.
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PubMed
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Lili Kong,Jie Chen,Meizhen Huang
In this paper, we have developed a graphene oxide/Au@Ag nanobones membrane (GO/Au@Ag NBs membrane) for simultaneous enrichment and SERS detection of colorants in beverages. Raman enhancement effect of Au@Ag nanobones (NBs) and enrichment capability of graphene oxide (GO) are contributed to the high sensitivity of GO/Au@Ag NBs membrane. Microporous membrane provides a support template for uniformly loading high-density GO/Au@Ag NBs. When beverage flow through the portable filtration unit with GO/Au@Ag NBs membrane, colorant molecules could be efficiently enriched. The prepared GO/Au@Ag NBs membrane exhibits an excellent sensitivity with a LOD of Rhodamine 6 G at 1.12 × 10 −9 mol/L, and has good performances of uniformity (with an RSD of 7.87 %), repeatability (with an RSD of 10.44 %), time stability (stored up to 60 days). Six types of colorants were sensitively detected combined with GO/Au@Ag NBs membranes, portable filtration unit and self-development portable Raman system. The lowest distinguished amounts of molecular accumulation are well below the maximum usage limits and the limits of visual recognition. Support vector machine model was established to identify the types of colorants, including colorants with coincident Raman peaks or similar visual colors, with an average accuracy of 94.8 %. In addition, GO/Au@Ag NBs membranes were used to enrich and detect colorant additives in commercial beverages, brilliant blue in energy beverage and carmine in bayberry wine were successfully detected. These results indicate GO/Au@Ag NBs membranes combined with a portable filtration unit can effectively enrich and rapidly detect colorants in beverages, and has great potential in the field of on-site and sensitive food safety detection.
研究领域:
分析化学
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食品安全检测
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纳米材料科学
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DOI
高影响因子
TALANTA
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2023
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IF 6.1
Chen Cui,Xinyao Lin,Jie Lv,Hang Guo,Lu Shen,Guoqiang Xiang,Wenjie Zhao,Dechen Jiang
Designing innovative electrochemiluminescence (ECL) immunosensors is critical for the detection of biomarkers with a low concentration and the precise evaluation of clinical diseases. Herein, a Cu 3 (hexahydroxytriphenylene) 2 (Cu 3 (HHTP) 2 ) nanoflake-based sandwich-type ECL immunosensor was constructed for C-Reactive Protein (CRP) detection. The Cu 3 (HHTP) 2 nanoflake , an electronically conductive metal–organic framework (MOF), has a periodically arranged porous structure with a cavity size of 2 nm, which not only accommodates a large amount of Ru(bpy) 3 2+ but also confines the spatial diffusion of active species. Therefore, the Ru(bpy) 3 2+ -loaded Cu 3 (HHTP) 2 nanocomplex (Ru@CuMOF) as an ECL emitter exhibits an enhanced ECL efficiency. The ECL resonance energy transfer (ECL-RET) was accomplished by combining Ru@CuMOF used as a donor with gold nanoparticles-functionalized graphene oxide nanosheets (GO-Au) utilized as an acceptor. This should be ascribed to the fact that the ECL emission spectrum of Ru@CuMOF shows the strongest signal intensity at 615 nm, overlapping with the absorption spectrum of GO-Au at 580–680 nm. Targeted detection of CRP in human serum samples was achieved by the sandwich-type immunosensor based on the ECL-RET mechanism with a 0.26 pg mL −1 detection limit. The electro-activated hybrids of Cu 3 (HHTP) 2 and ECL emitters provide a new sensing strategy for the high-sensitivity detection of disease markers.
研究领域:
生物医学工程
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分析化学
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临床诊断
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PubMed
高影响因子
Nanoscale
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2014
·Vol. 6
·期 15
·8535-8542
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IF 6.739
Xiangyan Zhou,Marco Dorn,Jürgen Vogt,Daniel Spemann,Wei Yu,Zhengwei Mao,Irina Estrela-Lopis,Edwin Donath,Changyou Gao
Noble-metal nanoparticles (NPs) especially prepared from gold and silver have been combined on the surface of graphene to obtain graphene-based nanocomposites for novel functions in enhanced performance in bio-imaging, cancer detection and therapy. However, little is known about their cellular uptake, especially the intracellular quantity which plays a critical role in determining their functions and safety. Therefore, we prepared covalently conjugated GO/Au and GO/Ag composites by immobilizing Au and Ag nanoparticles on GO sheets pre-functionalized with disulfide bonds, respectively. The cellular uptake of these composites was quantitatively studied by means of an ion beam microscope (IBM) to determine the metal content in human lung cancer cells (A549 cells) and liver hepatocellular carcinoma cells (HepG2 cells). The cell uptake was also studied by inductively coupled plasma mass spectrometry (ICP-MS), which is one of the most sensitive techniques being applied to cell suspensions, for comparison. Toxicity, one of the consequences of cellular uptake of GO based composites, was studied as well. The potential toxicity mechanism was also suggested based on the results of intracellular quantification of the nanomaterials.
研究领域:
纳米技术
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生物医学工程
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毒理学
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细胞生物学
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PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Lijun Yin,Peipei Xu,Junjie Ren,Jing Shi,Runbing Huang,Yunzhen Liu,Yudong Lu,Ruiyun You
Stonehouse clam toxin (STX) is a prevalent neuroparalytic toxin found among shellfish toxins . When consumed by humans, it disrupts neuronal conduction , potentially leading to quadriplegia , headache , shock, and even respiratory failure-induced death. This study introduces a highly sensitive and specific method for detecting shellfish toxins . The approach involves utilizing graphene gold-loaded compounds alongside stone house clam toxin aptamers and their complementary chains. The layered structure of graphene creates numerous hot spots among nanoparticles , while its arched spatial configuration offers an enhanced specific surface area , resulting in improved Surface-Enhanced Raman Scattering (SERS). By combining the signaling molecule Bodipy R6G-modified Apt with GO-Au loading compounds, distinctive Raman characteristic peaks emerge. Due to the strong affinity between Apt and STX, the Apt carrying the signaling molecule migrates away from the SERS aptamer probe's surface, thereby attenuating the original SERS signal intensity and facilitating ultra-sensitive shellfish toxin detection. The detection limit for the toxin was determined to be 5.47 nM, with a recovery rate ranging from 97.60 % to 108.32 %, affirming the applicability of this design for quantitative and specific shellfish toxin detection, signifying its considerable practical significance.
研究领域:
毒理学
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分析化学
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纳米技术
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DOI
高影响因子
Analytical Methods
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2019
·Vol. 11
·期 30
·3837-3843
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IF 2.378
Lili Xu,Hongbo Suo,Jianling Wang,Feixiang Cheng,Houmei Liu,Hongdeng Qiu
A novel magnetic graphene oxide modified with Au nanoparticles (Fe3O4@CS/GO/Au) as an efficient adsorbent was prepared for the detection of rhodamine B (RB) coupled with fluorescence spectrophotometry. The as-prepared Fe3O4@CS/GO/Au was characterized by using a field emission scanning electron microscope (SEM), energy dispersive X-ray spectrometer (EDS), and vibrating sample magnetometer (VSM). The main parameters, which affect extraction and desorption efficiencies, were systematically investigated and optimized. Under the optimal conditions, good linearities (0.1–6.0 μg L−1) with R2 = 0.9990, LODs (0.03 μg L−1) and LOQs (0.1 μg L−1) were achieved. Compared with chitosan-coated magnetic nanoparticles (Fe3O4@CS) and graphene oxide-modified Fe3O4@CS (Fe3O4@CS/GO), Fe3O4@CS/GO/Au exhibited optimal extraction efficiency. To demonstrate the application potency of Fe3O4@CS/GO/Au, it was successfully applied to isolate and extract RB from tap water, waste water, soft drink and eye shadow and the spiked recoveries were between 94.51% and 101.24% with RSD values from 1.95% to 6.15%. It was confirmed this novel method was an efficient pretreatment and enrichment procedure and could be successfully applied for the extraction and determination of trace RB in complex matrices.
研究领域:
材料科学
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分析化学
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环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）