JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Jialiang Pan,Chenhong Liu,Zhiqiang Wang,Wenbo Shi,Tianhao Zhao,Qingcong Wei,Qing Zhang,Zhengyu Bai
The hyperglycemic microenvironment of diabetic chronic wounds is a major driver of severe bacterial infections, excessive inflammatory responses, and impaired angiogenesis, all of which delay wound healing. To address these challenges, we prepared a glucose oxidase (GOx)–loaded zeolitic imidazolate framework-8 (GOx@ZIF-8). Furthermore, a multifunctional hydrogel (AACZ) was fabricated by incorporating 5-methylfurfural– and L-arginine–grafted alginate and maleimide–grafted chondroitin sulfate with GOx@ZIF-8 through the Diels–Alder reaction. Results showed that immobilized GOx remained active for a longer time than of free GOx. At the wound site, GOx catalyzed the conversion of excess glucose to gluconic acid and hydrogen peroxide (H 2 O 2 ). The reduced pH in the local region induced Zn 2+ release from zeolitic imidazolate framework-8, simultaneously, H 2 O 2 mediated the generation of nitric oxide (NO) from L-arginine. The synergy between Zn 2+ and NO endowed AACZ with excellent antibacterial properties. The biocompatible hydrogel promoted cell proliferation and migration, regulated local blood glucose levels, inhibited bacterial activity, alleviated inflammation and promoted angiogenesis and collagen deposition at the diabetic wound site. These findings suggest that AACZ holds promise as a responsive dressing material in healing for complex wounds.
研究领域:
生物材料
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纳米医学
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伤口愈合
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生物化学
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糖尿病研究
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
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IF 2.931
Zhenghong Zhao,Tianran Lin,Wenren Liu,Li Hou,Fanggui Ye,Shulin Zhao
Zeolitic imidazolate framework-8 (ZIF-8) has become one of the most typical examples of nanostructures for multi-enzyme immobilization due to its economical, mild and easy synthesis process. However, ZIF-8 nanocrystals are easily decomposed under acidic conditions. To solve this problem, the Fe-polydopamine (Fe-PDA) was bonded with ZIF-8 surface to form [email protected] hybrid shell with good stability. Based on this, we developed glucose [email protected] @Fe-PDA ( [email protected] @Fe-PDA) integrated nanozymes (INAzymes) with cascade reactions via a mild and environmentally friendly method. In order to synthesize the INAzymes, GOx was first embedded in ZIF-8 by coprecipitation ( [email protected] ), and then [email protected] was bonded with Fe-PDA, which acted as a peroxidase mimic. The [email protected] hybrid shell protected the INAzymes nanostructure from degradation under acidic conditions, which results in good chemical stability of the [email protected] @Fe-PDA. In the INAzymes system, glucose is converted to gluconic acid by GOx in the presence of oxygen to produce H 2 O 2 as an intermediate. The H 2 O 2 reacts rapidly with Fe-PDA to generate OH, which oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB). The UV absorbance of oxidized TMB is directly proportional to the glucose concentration, and has a good linear relationship in the range of 5.0–100.0 μM glucose with detection limit of 1.1 μM. The INAzymes system has been successfully applied to rapid colorimetric detection of blood glucose levels. The INAzymes system exhibits high catalytic activity, excellent sensitivity, and enhanced chemical stability, playing great promise in clinical diagnosis and biosensing.
研究领域:
生物化学
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纳米技术
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·Vol. 12
·期 4
·1007-1021
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IF 6.1
Qixin Zhou,Xiaodan Li,Nan Gao,Guixia Ling,Peng Zhang
Diabetic wounds in a state of high glucose are refractory to treatment and healing, especially if they are infected with bacteria. Herein, a novel nanocomposite (CIP/GOx@ZIF-8) was synthesized by loading ciprofloxacin hydrochloride (CIP) and glucose oxidase (GOx) into zeolitic imidazole framework-8 (ZIF-8) that exhibited good glucose sensitivity and catalytic activity. The high glucose in diabetic wounds could be decomposed into hydrogen peroxide (H2O2) and gluconic acid via the catalysis of GOx, which further destroyed CIP/GOx@ZIF-8 to release Zn2+ and cargos. The combination of glucose starvation, Zn2+, H2O2 and CIP could elevate the antibacterial effect and reduce bacterial resistance. Subsequently, the nanocomposite was fabricated into dissolving microneedles (CIP/GOx@ZIF-8 MNs) using polyvinylpyrrolidone (PVP). The microneedles exhibited good mechanical strength, puncture performance, dissolving performance, glucose responsiveness, antibacterial performance and biocompatibility. For in vivo wound healing, CIP/GOx@ZIF-8 MNs with good biosafety facilitated neovascularization and collagen deposition as well as reduced inflammation, and the wounds were almost healed after treatment. This multimodal therapeutic strategy is created to provide a unique treatment for infected diabetic wounds.
研究领域:
材料科学
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纳米技术
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生物医学工程
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药学
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伤口愈合
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）