The consensus Nglyco-X-S/T motif and a previously unknown Nglyco-N-linked glycosylation are necessary for growth and pathogenicity of Phytophthora
Summary Asparagine (Asn, N )-linked glycosylation within N glyco -X-S/T; X ≠ P motif is a ubiquitously distributed post-translational modification that participates in diverse cellular processes. In this work, N -glycosylation inhibitor was shown to prevent Phytophthora sojae growth, suggesting that N -glycosylation is necessary for oomycete development. We conducted a glycoproteomic analysis of P. sojae to identify and map N -glycosylated proteins and to quantify differentially expressed glycoproteins associated with mycelia, asexual cyst, and sexual oospore developmental stages. A total of 355 N -glycosylated proteins was found, containing 496 glycosites, potentially involved in glycan degradation, carbon metabolism, glycolysis, or other metabolic pathways. Through PNGase F deglycosylation assays and site-directed mutagenesis of a GPI transamidase protein (GPI16) upregulated in cysts and a heat shock protein 70 (HSP70) upregulated in oospores, we demonstrated that both proteins were N -glycosylated and that the N glyco -N motif is a target site for asparagine – oligosaccharide linkage. Glycosite mutations of Asn 94 N glyco -X-S/T in the GPI16 led to impaired cyst germination and pathogenicity, while mutation of the previously unknown Asn 270 N glyco -N motif in HSP70 led to decreased oospore production. In addition to providing a map of the oomycete N -glycoproteome, this work confirms that P. sojae has evolved multiple N -glycosylation motifs essential for growth.
危险品化学品经营许可证（带存储）