CELL BIOLOGY AND TOXICOLOGY
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2025
·Vol. 41
·期 1
·1-22
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IF 5.9
Meng Xinyue,Wei Xiaowei,Gu Hui,Ma Wei,Liu Dan,Jia Shanshan,Cao Songying,Ding Dongxue,He Yiwen,Huang Wanqi,Xue Jia,Luo Wenting,Sun Wei,Yuan Zhengwei
Duodenal atresia (DA) is a common neonatal digestive tract obstruction, with unclear prenatal diagnostic specificity and optimal intervention timing. tRNA-derived small RNAs (tsRNAs), stable and enriched in blood, are promising biomarkers for disease diagnosis. Therefore, identifying tsRNA biomarkers, elucidating DA pathogenesis, and exploring potential intrauterine interventions is urgently needed. This study conducts tsRNA profiling via sequencing on plasma samples from pregnant women carrying fetuses with DA and matched healthy controls. Validation was performed in 147 pregnant women, including cohorts with fetal gastrointestinal atresia, normal pregnancies, and post-delivery cases. Functional analyses in cellular models and Adriamycin rat models with DA explored the role of key tsRNAs in DA pathogenesis and intrauterine therapy. It is found that tsRNAs, including tRF-61:78-chrM. Leu-TAA, tRF-60:77-Ile-AAT-1-M4, tRF-57:76-Arg-ACG-1-M2, and tRF-58:76-Tyr-GTA-2-M3, were significantly downregulated in DA cases. tRF-58:76-Tyr-GTA-2-M3 is further implicated in DA development, with knockdown inducing excessive apoptosis via upregulation of SUFU and suppression of GLI1, a hedgehog pathway transcription factor. Intraperitoneal microinjection of tRF-58:76-Tyr-GTA-2-M3 agomir in DA rat models reduce apoptosis and mitigates DA formation by modulating SUFU and GLI1 expression. Taken together, this study identifies novel tsRNA biomarkers for DA, with tRF-58:76-Tyr-GTA-2-M3 playing a pivotal role in its pathogenesis. These findings offer insights into DA mechanisms and suggest potential therapeutic targets, advancing strategies for early diagnosis and intervention.
研究领域:
医学
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分子生物学
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产前诊断
✓ 已验证
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2016
·
IF 3.42
Shuang Gao,Zhiguo Yuan,Weimin Guo,Mingxue Chen,Shuyun Liu,Tingfei Xi,Quanyi Guo
The objectives of this study were to fabricate porous scaffolds using decellularized meniscus, and to explore a preferable crosslinking condition to enhance mechanical properties of scaffolds. Moreover, the microstructure, porosity, biodegradation and cytotoxicity were also evaluated. EDAC or GTA in different concentration was used to crosslink scaffolds. FTIR demonstrated functional groups change in crosslinking process. SEM photography showed that crosslinked scaffolds had blurry edges, which resulted scaffolds crosslinked by 1.2 mol/l EDAC had smaller porosity than other groups. The structure change enhanced antidegradation property. After immersing in enzyme solution for 96 h, scaffolds crosslinked by GTA and EDAC could maintain their mass > 70% and 80%. Most importantly, mechanical properties of crosslinked scaffolds were also improved. Uncrosslinked Scaffolds had only 0.49 kPa in compression modulus and 12.81 kPa in tensile modulus. The compression and tensile modulus of scaffolds crosslinked by 1.0% GTA were 1.42 and 567.44 kPa respectively. The same value of scaffolds crosslinked by 1.2 mol/l EDAC were 1.49 and 532.50 kPa. Scaffolds crosslinked by 1.0% and 2.5% GTA were toxic to cells, while EDAC groups showed no cytotoxicity. Chondrocytes could proliferate and infiltrate within scaffolds after seeding. Overall, 1.2 mol/l EDAC was a preferable crosslinking condition.
研究领域:
组织工程学
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 105
·60460-60466
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IF 3.708
Shuxian Huang,Zuoyi Xiao,Shangru Zhai,Bin Zhai,Feng Zhang,Qingda An
To enhance the stability of a catalyst that uses alginate as a carrier, Ag nanoparticle-loaded glutaraldehyde-crosslinked calcium alginate (Ag/CA@GTA) hydrogel beads were prepared. The Ag/CA@GTA hydrogel beads were characterized by UV-visible spectroscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, transmission electron microscopy and X-ray photoelectron spectroscopy analyses. Given that the mechanical strength of the support was remarkably enhanced by crosslinking alginate with GTA, the resulting Ag/CA@GTA beads exhibited high stability and durable activity, with more than 97% conversion within 10 successive cycles, for the catalytic reduction of 4-nitrophenol to 4-aminophenol by NaBH4 in aqueous solution. These Ag/CA@GTA hydrogel beads were expected to have potential as a highly efficient, cost-effective and eco-friendly heterogeneous catalyst for industrial applications.
研究领域:
材料科学
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催化化学
✓ 已验证
产品关联
DOI
高影响因子
Energy & Environmental Science
·
2022
·Vol. 15
·期 12
·5379-5390
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IF 39.714
Yuchen Li,Qikai Li,Xinbo Zhang,Jiajia Zhang,Shuaihua Wang,Liuqin Lai,Kang Zhu,Weishu Liu
Quasi-solid-state ionic thermoelectric (i-TE) cells have attracted increasing interest due to their high thermopower and easy solution processability for flexible and wearable thermoelectric devices. However, many i-TE gels have drawbacks of a narrow working temperature range (ΔTmax) and low power density. In this study, we introduced glutaraldehyde into the gelatin-KCl-FeCN4−/3− matrix, forming strong covalent bonds and interconnected porous structures, which significantly improve ΔTmax from 9 °C to 23 °C. The formed gelatin/GTA polymer network increases the entropy difference between the redox couples of FeCN4−/3−, thereby enhancing its thermopower. For the first time, a high thermopower of 24.7 mV K−1, a record-high power density of 9.6 mW m−2 K−2 and a 2 h energy density (E2h) of 198 J m−2 are achieved simultaneously in a quasi-solid-state i-TE cell. The i-TE cell exhibits good cycling performance in long-term power generation, corresponding to an average E2h value of 175 J m−2 after the whole cycling process. A flexible and wearable device consisting of 16 i-TE cells can generate a high voltage of 3.6 V and an output power of 115 μW by harvesting body heat. This work provides a general route to increase the working temperature range and output power density of gel-based i-TE cells through a molecular-level approach.
研究领域:
材料科学
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能源技术
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 26
·2300710
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IF 19
Yaodong Zhao,Bingcheng Yi,Jilin Hu,Dongjie Zhang,Guotai Li,Yun Lu,Qihui Zhou
When skin trauma occurs, rapid achievement of the post-wound closure is required to prevent microbial invasion, inhibit scar formation and promote wound healing. To develop a wound dressing for accelerating post-wound-closure and wound healing, a thermo-responsive and tissue-adhesive hydrogel with interpenetrating polymer networks (IPN) is fabricated based on N -dimethylbisacrylamide (NIPAM) and glutaraldehyde (GTA) cross-linked hyaluronic acid (HA). Results not only confirm the thermo-stimulated self-contraction and tissue adhesiveness of the HA-based IPN (PNI-HA), which effectively aids wound closure via mechanical stretch, but also verify the hemocompatibility and cytocompatibility of PNI-HA that tend to accelerate wound healing. In vivo, a mouse model of total skin defect demonstrates that PNI-HA acting as hydrogel sealant significantly achieves the sutureless post-wound-closure at the early stage of wound healing, and then promotes wound healing by reducing inflammatory cells infiltration, promoting angiogenesis as well as reducing collagen deposition. These results indicate that the developed thermo-responsive and tissue-adhesive hydrogel dressing offers a candidate to serve as a tissue sealant for wound healing.
研究领域:
生物材料
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组织工程
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伤口愈合
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
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