JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jiaxin Li,Lianghua Xie,Zishan Dou,Yiyang Zhou,Jianling Mo,Wei Chen
Cultured meat technology is expected to solve problems such as resource shortages and environmental pollution, but the muscle fiber differentiation efficiency of cultured meat is low. Genipin is the active compound derived from Gardenia jasminoides Ellis, which has a variety of activities. Additionally, genipin serves as a noncytotoxic agent for cross-linking, which is suitable as a foundational scaffold for in vitro tissue regeneration. However, the impact of genipin on myoblast differentiation remains to be studied. The research revealed that genipin was found to improve the differentiation efficiency of myoblasts. Genipin improved mitochondrial membrane potential by activating the AMPK signaling pathway of myoblasts, promoting mitochondrial biogenesis, and mitochondrial network remodeling. Genipin activated autophagy in myoblasts and maintained cellular homeostasis. Autophagy inhibitors blocked the pro-differentiation effect of genipin. These results showed that genipin improved the differentiation efficiency of myoblasts, which provided a theoretical basis for the development of cultured meat technology.
研究领域:
细胞生物学
·
分子生物学
·
再生医学
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2025
·
IF 4.8
Yu Deng,Ruobing Fu,Yaqi Shang,Mengting Hu,Xirui Xin,Yubo Hou,Weixian Yu,Xinchan Liu
Introduction: Periodontitis is a chronic inflammatory disease closely associated with mitochondrial dysfunction. Uncoupling protein 2 (UCP2), located in the inner membrane of mitochondria, reduces mitochondrial membrane potential (MMP) and adenosine triphosphate (ATP) synthesis by promoting proton leakage across the membrane. This leads to decreased energy metabolism efficiency, impairing cellular glucose uptake, and disrupting intracellular energy balance. Genipin (GP), a recognized UCP2 inhibitor, exhibits anti-inflammatory and antioxidant properties. This study aimed to investigate the specific role of GP in periodontal tissue redox signaling and the potential mechanism of UCP2 in the development of periodontitis.Methods: In this study, we constructed a model of H2O2-induced oxidative stress in human periodontal ligament cells (hPDLCs). In vivo, a rat periodontitis model was established to evaluate the effects and mechanisms of GP in alleviating oxidative damage in periodontal tissues and cells.Results: Cell experiments showed that GP effectively alleviated H2O2-induced mitochondrial dysfunction and oxidative damage in hPDLCs by inhibiting UCP2 expression and function, restoring cell viability, and reducing cell apoptosis. Additionally, GP intervention increased the expression of glucose transporter 4 (GLUT4), thereby promoting cellular glucose uptake. The results of animal experiments demonstrated that GP intervention reduced alveolar bone resorption and periodontal tissue destruction in rats with periodontitis, inhibited osteoclast differentiation, improved mitochondrial dysfunction in periodontal tissue, promoted GLUT4 expression, and reduced oxidative stress levels and cell apoptosis.Discussion: GP regulates oxidative damage in periodontal tissues by maintaining mitochondrial homeostasis, promoting glucose transporter expression, and enhancing glucose uptake, with UCP2 playing a central role.
研究领域:
口腔医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2024
·
IF 3.9
Agha Hasan M.,Abdulhameed Ahmed Saud,Jawad Ali H.,Khadiran Tumirah,ALOthman Zeid A.,Wilson Lee D.
A biopolymer matrix of chitosan (CTS) and algae (AGA) was chemically modified with a natural genipin (GEN) cross-linker agent via hydrothermal process. The resulting green adsorbent (CTS-GEN/AGA) was evaluated for removal of the cationic dye (methyl violet, MV) from aqueous media. The optimization of MV adsorption onto CTS-GEN/AGA was performed using the Box–Behnken design (BBD), considering the variables of adsorbent dose (0.02–0.1 g/100 mL), pH (4–10), and time (20–300 min). The CTS-GEN/AGA demonstrated the highest MV removal (76.49%) for optimal operational parameters: CTS-GEN/AGA dosage (0.0938 g/100 mL), pH (8.4), and contact time (215.3 min). The adsorption isotherm analysis revealed a close fit between the experimental data of MV adsorption and the Temkin model. Furthermore, the adsorption kinetics are well-described by the pseudo-second-order model. The maximum adsorption capacity of CTS-GEN/AGA was 71.9 mg/g at 25 °C. The probable adsorption mechanism can be assigned to H-bonding, electrostatic forces, and n–π stacking interactions. These findings highlight the potential of CTS-GEN/AGA as an effective adsorbent for treatment of wastewater for the removal of organic dyes.
研究领域:
环境科学
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材料科学
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生物化学
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产品关联
DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 10
·2632-2642
·
IF 2.137
Wen Liu,Xiao Yang,Na Li,Guanghui Xi,Mingshan Wang,Bin Liang,Yakai Feng,Hao Chen,Changcan Shi,Wenzhong Li
Efficient hemorrhage control is of extreme importance to decrease mortality rates arising from traumatic bleeding. However, traditional hemostatic agents are limited to unsatisfied biocompatibility, inconvenient usage, and high cost. To this end, we have devised a novel hemostatic microsphere from chitosan and gelatin by using appropriate amount of genipin as a crosslinker. The performance of these Cs/Gel MPs was evaluated with experiments in vitro and in vivo. Results in vitro showed that the best hemostatic efficacy was achieved by Cs/Gel-0.75 MPs (the mass fraction of genipin in Cs/Gel mixture is 8.6 × 10 −6 ). Skin laceration model indicated that the hemostatic time of Cs/Gel-0.75 MPs was within 12 seconds, which has been significantly shortened in comparison with commercial available products. Moreover, cytocompatibility results displayed that Cs/Gel-0.75 MPs caused no obvious cytotoxicity even at the high concentration of 40 μg/ml. Cs/Gel-0.75 MPs also possess satisfactory fluid absorption ratio and low hemolysis. Therefore, these Cs/Gel-0.75 MPs are promising candidates for further surgical wound hemostatic agent.
研究领域:
生物医学工程
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lingling Sha,Husnain Raza,Chengsheng Jia,Imran Mahmood Khan,Huqing Yang,Gang Chen
Polysaccharide-protein nanocomplexes are considered one of the desired systems to encapsulate bioactive compounds. The study prepared chitosan (CS)/genipin (GP)/zein nanoparticles with chemical cross-linkage for encapsulating and releasing curcumin. Fourier transform infrared spectra demonstrated that cross-linkage between molecules was attributed to electrostatic interactions and the formation of amido bonds and hydrogen bonding. Transmission electron microscopy showed that all prepared nanoparticles showed a spherical morphology. Particularly, cinnamaldehyde screening –NH 2 groups in CS significantly reduced the encapsulation efficiency of nanoparticles, demonstrating the significant role of the –NH 2 group in CS-coated zein nanoparticles. Additionally, the encapsulation efficiency was largely increased to 82.3 % at the ratio of CS/GP/zein ( w /w, 20:1:100), compared with 38.4 % using zein as a single encapsulation for curcumin. In vitro digestion, the addition of GP and CS decreased the release of curcumin from 84.8 % to 57.1 % after the initial digestion of 2 h. The GP/CS/zein particles retained 12 % of curcumin after 6- h of digestion, indicating the improvements in encapsulation efficiency and release properties.
研究领域:
食品科学
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生物材料学
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DOI
PubMed
高影响因子
Biomedical Materials
·
2024
·Vol. 19
·期 4
·045042
·
IF 3.9
Ling Wang,Xiaoyue Ding,Jingyu Li,Man Li,Peng Ding,Wei Guo,Qiaoyun Wu,Yanfang Sun,Guohua Jiang,Oseweuba Valentine Okoro,Mahta Mirzaei,Amin Shavandi,Lihong Fan,Lei Nie
Bacterial infection can lead to various complications, such as inflammations on surrounding tissues, which can prolong wound healing and thus represent a significant clinical and public healthcare problem. Herein, a report on the fabrication of a novel genipin/quaternized chitosan (CS) hydrogel for wound dressing is presented. The hydrogel was prepared by mixing quaternized CS and genipin under 35 °C bath. The hydrogels showed porous structure (250–500 μm) and mechanical properties (3000–6000 Pa). In addition, the hydrogels displayed self-healing ability and adhesion performance on different substrates. Genipin crosslinked quaternized CS hydrogels showed antibacterial activities against E. coli and S. aureus. The CCK-8 and fluorescent images confirmed the cytocompatibility of hydrogels by seeding with NIH-3T3 cells. The present study showed that the prepared hydrogel has the potential to be used as wound dressing.
研究领域:
生物材料学
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医学工程
·
药剂学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2018
·
IF 1.911
Longfei Li,Weizheng Zhang,Mengjia Huang,Jie Li,Jia Chen,Mi Zhou,Jianguo He
The wound healing for tympanic membrane (TM) perforations is one of the most common clinical treatment in otomicrosurgery. Recently, tissue-engineered composites and grafts, also new designs of biomaterials are applied to the management of TM perforation. In this work, a series of gelatin/genipin nanofibrous films were prepared as a patch for repairing TM perforation. Gelatin, a type of biomaterial with excellent electrospinning performance, has been used for preparing the nanofibers. The genipin, as a crosslinking agent, has been blended into the gelatin nanofibers. The reaction between gelatin and genipin engender suitable tensile strength and water-resistance for TM patch. The survival rate of human umbilical vein endothelial cells and fibroblasts demonstrated that the gelatin/genipin nanofiber scaffolds had good biocompatibility, which indicated the genipin was a kind of effective and nontoxic crosslinking agent for improving the mechanical property and water-resistance of gelatin films. In short, our work provides a novel macromolecular material with good mechanical properties, water-tolerance and excellent biocompatibility which could be used as a potential patch for TM repair.
研究领域:
生物材料学
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组织工程学
·
耳科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 9
·2906
·
IF 4.927
Shuying Han,Zhaohua Zhang,Jia Chen,Jie Li,Mi Zhou,Zejian He,Zhen He,Longfei Li
Tympanic membrane perforation (TMP), a common disease, often needs a scaffold as the patch to support surgery. Due to the environment of auditory meatus, the patch can be infected by bacteria that results in failure; therefore, the ideal scaffold may combine biomimetic and antibacterial features. In this work, gelatin was used as the electrospinning framework, genipin as the crosslinking agent, and levofloxacin as an antibacterial in order to prepare the scaffold for TMP. Different contents of levofloxacin have been added to gelatin/genipin. It was found that, with the addition of levofloxacin, the gelatin/genipin membranes exhibit improved hydrophilia and enhanced tensile strength. The antibacterial and cell-cultured experiments showed that the prepared antibacterial membranes had excellent antibacterial properties and good biocompatibility, respectively. In summary, levofloxacin is a good group for the gelatin/genipin scaffold because it improves the physical properties and antibacterial action. Compared with different amounts of levofloxacin, a gelatin/genipin membrane with 1% levofloxacin is more suitable for a TM.Keywords:gelatin;genipin;levofloxacin;nanofiber membrane;tympanic membrane
研究领域:
生物材料学
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耳科学
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组织工程学
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产品关联
DOI
PubMed
高影响因子
Catalysts
·
2021
·Vol. 11
·期 10
·1158
·
IF 4.146
Zhanyong Wang,Tingting Su,Jingjing Zhao
Genipin was used as a crosslinking agent to prepare magnetic genipin-crosslinked chitosan beads, which were then used as a carrier for immobilizing recombinant cutinase fromFusarium solani(FSC) to obtain immobilized FSC. The optimal temperature for the immobilized FSC was 55 °C, which was 5 °C higher than that of the free enzyme, whereas its optimal pH was increased from 8.0 to 9.0; this indicates that the immobilized FSC had improved pH and thermal stability. After repeated use for 10 cycles, the activity of the immobilized FSC remained at more than 50%; after being stored at 4 °C for 30 days, its activity was still approximately 88%. We also found that the Km of the immobilized FSC was higher than that of the free enzyme. These results indicate that the performance of FSC was improved after immobilization, which is an important basis for the subsequent application of FSC in industrial production.Keywords:immobilization;cutinase;chitosan;genipin
研究领域:
生物技术
·
酶学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
npj Science of Food
·
2025
·Vol. 9
·期 1
·1-12
·
IF 7.8
Chen Lijia,Zhang Wenjie,Bian Qiqi,Wang Xichang,Zhong Jian
The application of fish oil is limited by its unpleasant fishy flavour, sensitivity to environments (e.g., oxygen, light, and heat), and poor water solubility. The complex coacervation-based encapsulation of fish oil in powders is a promising method to improve the behavior of fish oil in food science and engineering. Controlled preparation of fish oil powders has become an important topic in improving their properties. Herein, the fish oil-encapsulated powders were controlled prepared by combining a fish gelatin (FG)-alginate complex coacervation method with an intra-particulate genipin-crosslinking method. The genipin crosslinking did not affect the particle morphologies, moisture contents, and fish oil encapsulation ability. However, it did affect other physicochemical properties, fish oil oxidation ability, and in vitro digestion behaviors. Especially, the genipin-to-FG mass ratio of 1.0% induced the lowest water activity (0.45 ± 0.01), highest tapped density (0.107 ± 0.007 g/cm 3 ), highest Carr indexes (58% ± 2%), highest Hausner ratios (2.43 ± 0.13), lowest peak value (636.0 ± 10.0 μm) of the powder sizes, and lowest peroxide peak value (442 ± 3 mmol/kg oil). This work provided useful information to understand the relationship between genipin modification and the properties of the fish oil-encapsulated powders.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2010
·
IF 3.167
Yanni Zhang,Ying Yang,Tianying Guo
In this work chitosan microspheres have been prepared by ion precipitation using sodium sulfate as precipitant, and crosslinked with natural crosslinker genipin . Then the microspheres were hydrophobically modified by covalent and ion interaction grafting of stearic acid and sodium stearate respectively. Results indicated that the there were still some amount of –NH 2 groups on the genipin-crosslinked chitosan microspheres. And the contact angles for hydrophobical graft microspheres were increased greatly from 27° to 104°, which indicates stearic chains have been grafted on the surface of the microspheres even only by a simple graft process based on ion interaction. The interactions of protein with the hydrophobically modified chitosan microspheres were investigated by surface absorption method using bovine serum albumin as a model protein. And results indicated that adsorption efficiency for bovine serum albumin of the fatty chain grafted chitosan microspheres was higher than that of the prestine one distinctly, due to the additional hydrophobic interaction. And the adsorbed protein could well-off be desorbed under a general condition.
研究领域:
生物材料学
·
蛋白质科学
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-12
·
IF 5.228
Sun Fei,Jiang Yuan,Xu Yanfei,Shi Hongcan,Zhang Siquan,Liu Xingchen,Pan Shu,Ye Gang,Zhang Weidong,Zhang Fangbiao,Zhong Chonghao
Decellularization techniques have been widely used as an alternative strategy for organ reconstruction. This study investigated the mechanical, pro-angiogenic and in vivo biocompatibility properties of decellularized airway matrices cross-linked with genipin. New Zealand rabbit tracheae were decellularized and cross-linked with genipin, a naturally derived agent. The results demonstrated that, a significant (p < 0.05) increase in the secant modulus was computed for the cross-linked tracheae, compared to the decellularized samples. Angiogenic assays demonstrated that decellularized tracheal scaffolds and cross-linked tracheae treated with 1% genipin induce strong in vivo angiogenic responses (CAM analysis). Seven, 15 and 30 days after implantation, decreased (p < 0.01) inflammatory reactions were observed in the xenograft models for the genipin cross-linked tracheae matrices compared with control tracheae and no increase in the IgM or IgG content was observed in rats. In conclusion, treatment with genipin improves the mechanical properties of decellularized airway matrices without altering the pro-angiogenic properties or eliciting an in vivo inflammatory response.
研究领域:
组织工程
·
生物材料
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）