INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Yang Yang,Ru Zhao,Hongfei Gao,Zehao Wang,Xinyu Yang,Ming Ruan,Huiyan Gu,Lei Yang,Hao Tian,Changwen Fan,Tingting Liu
In this study, secoiridoids (gentiopicroside, loganic acid, swertiamaroside, and sweroside) were extracted from Gentiana rigescens roots and rhizomes with β-cyclodextrin (β-CD) as the booster and biobased glycine-glycerol deep eutectic solvent (DES) as the extraction solvent and with ultrasound-assisted extraction. The molecular docking performances showed that in DES aqueous solution, β-CD interacts with secoiridoids through noncovalent bonds and hydrogen bonds, and the four secoiridoids studied can be well adapted to the hydrophobic cavity of β-CD. The hydrogen bonds present between secoiridoids and β-CD all reached more than four, and the corresponding binding energies were all lower than − 5.0 kcal/mol. The yields of gentiopicroside, loganic acid, swertiamaroside, and sweroside were 39.82 ± 2.05 mg/g, 6.61 ± 0.34 mg/g, 0.79 ± 0.04 mg/g, and 0.57 ± 0.04 mg/g, respectively, under optimal extraction conditions. The results showed that ultrasound-assisted extraction of secoiridoid compounds in G. rigescens with an aqueous β-CD/DES solution was more effective compared with conventional extraction solvents, which provides a scientific basis for the promising use of β-CD/DES as extraction solvents in natural drugs.
研究领域:
植物化学
·
天然药物化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Rui Zhang,Caixia Yan,Xiaofang Yang,Kan Hu,Fangfang Hao,Shengchun Yang,Qingwen Deng,Zhenjuan Duan,Yong Liu,Xiaodong Wen
In this work, ultrasound-assisted rapidly synergistic cloud point extraction (UARS-CPE) and inductively coupled plasma optical emission spectrometry (ICP-OES) were combined to determine trace Pb in Gentiana rigescens Franch. ex Hemsl. ( G. rigescens ) samples. Under the optimal conditions, the enhancement factor (EF), limit of detection (LOD), limit of quantitation (LOQ) and precision were 33, 0.11 μg L −1 , 0.37 μg L −1 and 1.3%, respectively. This method was applied to the analysis of G. rigescens samples, and the outcomes were in good agreement with the results determined by inductively coupled plasma mass spectrometry (ICP-MS). A mice model of immune liver injury induced by concanavalin A (ConA) was established, and the liver protection of G. rigescens and gentiopicroside (GPS) on it and the effects of various dosages of Pb exposure on its liver protection were studied. Pb at a dosage of 5 mg kg −1 had little effect on the liver protection of G. rigescens and GPS, while 25, 125 mg kg −1 dosages of Pb could significantly attenuate the liver protection of both. In addition, it aggravated the necrosis of hepatocytes and inflammatory cell infiltration , and these effects were dose-dependent.
研究领域:
分析化学
·
药理学
·
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Ruoxin Chen,Jiahao Ren,Zhenlong Zhou,Wenjun Zhang,Jianyu Su
Gentiana rigescens Franch. extract have demonstrated various important bioactivities, however, their potential against photoaging has yet to be evaluated. This study explored the anti-photoaging effects of Gentiana rigescens Franch. root extract (GRFR) and stem (GRFS), along with their underlying mechanisms and bioactive components, using the UVB-induced zebrafish model. GRFS demonstrated superior anti-photoaging efficacy compared to GRFR. GRFS significantly alleviated UVB-induced tail fin damage, increasing the relative tail fin area from 31.23% to 80.92% (at 1mg/mL), and reducing ROS levels from 244.80% to 150.95%. It also inhibited lipid peroxidation, lowering malondialdehyde (MDA) levels from 202.87 nmol/g to 142.83 nmol/g. Furthermore, GRFS enhanced antioxidant enzyme activities, increasing superoxide dismutase (SOD) from 80.94 U/g to 104.06 U/g. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) indicated substantial distinctions in the composition and content of major constituents between GRFR and GFRS, which may explain the differences in their anti-photoaging activities. And then network pharmacology was used to investigate possible mechanisms of GFRS, which identified five potential targets including CTNNB1, ESR1, AKT1, JUN and EGFR, as well as three key active compounds including aspartyl-phenylalanine (Asp-Phe), N-acetyl-L-phenylalanine (N-Acetyl-L-Phe), and phenylalanyl-tyrosine (Phe-Tyr). Finally, the molecular docking revealed that Phe-Tyr exhibited the strongest binding affinity for AKT1 (-8.86 kcal/mol), indicating that it may be the most potent anti-photoaging compound in GFRS. These findings suggest that G . rigescens , with its potent anti-photoaging properties, offers superior protection against UVB-induced photoaging and holds promise as a natural therapeutic agent.
研究领域:
药理学
·
植物学
·
皮肤科学
·
生物化学
·
计算生物学
·
天然产物研究
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）