Food Bioscience
·
2025
·
IF 5.9
Tingwen Wang,Gang Yang,Kewei Chen,Muying Du,Zsolt Zalán,Ferenc Hegyi,Sameh Awad,Nassra Dabou,Amel Ahmed Ibrahim,Jianquan Kan
Patulin (PAT) is a neurotoxic fungal metabolite that commonly contaminates fruits and their derivatives, causing substantial economic loss and serious harm to human health. In this study, we screened a PAT-degrading yeast strain, Geotrichum candidum XG1, among 12 probiotic strains. It efficiently degraded 96.80 % of PAT (5 μg/mL) within 48 h in potato dextrose broth and completely degraded PAT in fresh apple juice. G. candidum XG1 degraded PAT mainly by secreting intracellular enzymes, which were induced by PAT, and the degradation rate increased with increasing induction time. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry identified ascladiol as a PAT degradation product that disappeared within 96 h. Notably, both G. candidum XG1 and its intracellular enzymes affected the quality of apple juice, and G. candidum XG1 enhanced the flavor of apple juice. Overall, these results highlight the potential of G. candidum XG1 to remove PAT from apple juice.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2022
·Vol. 109
·期 6
·1043-1050
·
IF 2.807
Qv Wenrui,Wang Xilin,Li Ningyun,Du Jingjing,Pu Gaozhong,Zhang Hongzhong
In this study, exposure experiments were conducted to assess the effects of polystyrene nanoparticles (PS) and amine-modified polystyrene nanoparticles (APS) at environmental concentrations (1, 10, and 100 µg L − 1 ) on two fungal species ( Geotrichum candidum and Aspergillus niger ), isolated from leaf litter in streams, concerning their growth and metabolic activity. Results showed that PS at 1 and 10 µg L − 1 have hormesis effects on G. candidum growth. Compared with G. candidum , A. niger had higher sensitivity to nanoplastic exposure. Besides, the peroxidase and cellobiohydrolase activities of A. niger were significantly inhibited by nanoplastics (except 1 µg L − 1 PS), which would weaken its metabolic activity in carbon cycling. These results provided a new thought on how the growth and functions of aquatic fungi cope with the stress induced by nanoplastics. Overall, the study provided evidence for the different responses of aquatic fungi to nanoplastics in streams.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Gang Yang,Bin Li,Kewei Chen,Muying Du,Zsolt Zalán,Ferenc Hegyi,Jianquan Kan
Identifying aflatoxin-detoxifying probiotics remains a significant challenge in mitigating the risks associated with aflatoxin contamination in crops. Biological detoxification is a popular technique that reduces mycotoxin hazards and garners consumer acceptance. Through multiple rounds of screening and validation tests, Geotrichum candidum XG1 demonstrated the ability to degrade aflatoxin B 1 (AFB 1 ) by 99–100%, exceeding the capabilities of mere adsorption mechanisms. Notably, the degradation efficiency was demonstrably influenced by the presence of copper and iron ions in the liquid medium, suggesting a potential role for proteases in the degradation process. Subsequent validation experiments with red pepper revealed an 83% reduction in AFB 1 levels following fermentation with G. candidum XG1. Furthermore, mass spectrometry analysis confirmed the disruption of the AFB 1 furan ring structure, leading to a subsequent reduction in its toxicity. Collectively, these findings establish G. candidum XG1 as a promising candidate for effective aflatoxin degradation, with potential applications within the food industry.
研究领域:
食品科学
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Gang Yang,Xinrui Zhang,Muying Du,Kewei Chen,András Nagy,Krisztina Takács,Ferenc Hegyi,Amel Ahmed Ibrahim,Nassra Dabour,Sameh Awad,Zsolt Zalán,Jianquan Kan
Corn is highly susceptible to pathogen invasion, such as Aspergillus flavus , leading to aflatoxin contamination and posing a significant threat to food safety. Yeasts are widely applied in the food industry, and the use of yeasts for pathogen control as an alternative to physical and chemical methods has gained increasing attention. Previous studies have investigated the aflatoxin-degrading capability of Geotrichum candidum XG1 (an unconventional yeast), but its antifungal activity and application potential have not been explored. In this study, the antifungal activity of G. candidum XG1 was evaluated both in vitro and in vivo , and its impact on corn quality was investigated. The mechanisms and safety of the yeast were also further examined. The results indicated that G. candidum XG1 significantly inhibited A. flavus , reducing the fungus and aflatoxin contamination by over 90% on corn. A 75-day storage experiment assessing changes in corn color, starch, protein, fat, and fatty acid values revealed that G. candidum XG1 had minimal impact on corn quality. Compared to the control group (infected with A. flavus ), it significantly reduced the quality deterioration caused by A. flavus invasion. Gas chromatography-mass spectrometry (GC-MS) analysis identified phenylethyl alcohol as a key compound involved in the antifungal activity of the yeast. Phenylethyl alcohol inhibited A. flavus by affecting its cell morphology, energy metabolism, and defense systems. Safety evaluations, including whole-genome sequencing and animal trials, confirmed the safety of the strain. This study provides valuable insights into the application of antagonists like G. candidum XG1 for controlling pathogen in corn.
✓ 已验证
产品关联
DOI
高影响因子
BIOLOGICAL CONTROL
·
2020
·
IF 2.754
Shupei Wang,Yahan Zhou,Wei Luo,Lili Deng,Shixiang Yao,Kaifang Zeng
The present study investigated the effects of oligochitosan, salicylic acid (SA), and Pichia membranaefacien s on inducing disease resistance against Geotrichum candidum in citrus fruit by using iTRAQ proteomic and physico-chemical analysis. Our finding showed that with pre-treatment of oligochitosan, SA and P. membranaefaciens , disease incidences and lesion diameter infected by G. candidum were both significantly reduced. Proteomics analysis indicated that the differentially expressed proteins (DEPs) were significantly enriched in the pathways of starch and sucrose metabolism, carbon metabolism and amino acid metabolism induced by the three elicitors. Primary metabolites analysis showed that the three elicitors significantly stimulated the accumulation of primary metabolites that closely associated with the defense response such as hexoses, xylose, oxalic acid, proline, glutamate, aspartate, cysteine, etc. Moreover, the marked accumulation of organic acid such as citric acid, α-ketoglutarate, succinic acid, malic acid and fumaric acid which affect the TCA cycle was also observed in three elicitors treated citrus. In conclusion, primary metabolites induced by oligochitosan, SA and P. membranaefaciens behaved as the source of energy and substrates or signaling molecules that directly or indirectly participated in defense responses to against sour rot in citrus fruit.
研究领域:
植物病理学
·
蛋白质组学
·
代谢组学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）