Chemical Composition and Antioxidant/Antimicrobial Activities in Supercritical Carbon Dioxide Fluid Extract of Gloiopeltis tenax
Gloiopeltis tenax(G. tenax) is widely distributed along the Chinese coastal areas and is commonly used in the treatment of diarrhea and colitis. This study aimed at investigating the bioactivities of the volatile constituents inG. tenax. We extracted the essential constituents ofG. tenaxby supercritical carbon dioxide extraction (CO2-SFE), then identified and analyzed the constituents by gas chromatography-mass spectrometry (GC-MS). In total, 30 components were identified in theG. tenaxextract. The components showed remarkable antioxidant activity (radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH)), lipid peroxidation inhibition capacity (in a β-carotene/linoleic acid-coupled oxidation reaction), and hydroxyl radical-scavenging activity (by deoxyribose degradation by iron-dependent hydroxyl radical), compared to butylated hydroxytoluene. In microdilution assays,G. tenaxextracts showed a moderate inhibitory effects onStaphyloccocus aureus(minimum inhibitory concentration (MIC) = 3.9 mg/mL),Enterococcus faecalis(7.8 mg/mL),Pseudomonas aeruginosa(15.6 mg/mL), andEscherichia coli(3.9 mg/mL). Antioxidant and antimicrobial activities ofG. tenaxwere related to the active chemical composition. These results suggest that the CO2-SFE extract fromG. tenaxhas potential to be used as a natural antioxidant and antimicrobial agent in food processing.Keywords:Gloiopeltis tenax;GC-MS;CO2-SFE;antioxidant activity;antimicrobial activity
危险品化学品经营许可证（带存储）