INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Li Tang,Xiaomin Luo,Meiyuan Wang,Zhong Wang,Juan Guo,Fansheng Kong,Yongguang Bi
Under a simple redox system of selenite and ascorbic acid , we used Gracilaria lemaneiformis polysaccharides (GLPs) as a stabilizer and dispersing agent to generate well-dispersed and stable selenium nanoparticles (SeNPs). The size, stability, morphology and physicochemical properties of GLPs-SeNPs were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Ultraviolet-visible spectroscopy (UV–vis), Fourier transform infrared (FT-IR) spectra, energy dispersive X-ray (EDX), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Thermogravimetric (TG). The results showed that orange-red, amorphous, zero-valent and spherical GLPs-SeNPs with mean diameter of approximately 92.5 nm were successfully prepared, which exhibited good storage stability at 4 °C and remaining highly stable at different ion strengths and pH. The 2,2-diphenyl-1-pycrylhydrazyl (DPPH), 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) and superoxide anion radical (O 2 •− ) radical scavenging ability of GLPs-SeNPs were higher than those of bare SeNPs, GLPs and sodium selenite (Na 2 SeO 3 ), and could reach 103.41%, 94.23%, 86% at a concentration of 1.5 mg/mL, respectively. Besides, GLPs-SeNPs also showed higher inhibitory effects on α-amylase and α-glucosidase. In vitro cytotoxicity assay and hemolysis activity examinations indicated that GLPs-SeNPs have excellent biocompatibility. Therefore, the GLPs-SeNPs might be used as a potential antioxidant agent and antidiabetic agent for food and medical applications.
研究领域:
食品科学
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生物化学
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药理学
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DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2020
·Vol. 11
·期 6
·2899-2908
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IF 2.602
Yuan Shengliang,Wang Pan,Xiao Le,Liang Yan,Huang Yongmei,Ye Hua,Wu Kefeng,Lu Yingnian
During the agar production of the red seaweed Gracilaria lemaneiformis , amounts of lipid constituents are destroyed and discarded as wastes, which can be utilized as sustainable alternative feedstocks for lipid production. Herein, a green ultrasound-assisted extraction (UAE) method using ethanol as an extraction solvent was carried out for the enrichment of lipids from the wastes. Meanwhile, the UAE-influenced conditions were optimized by response surface methodology (RSM), and the maximum yield (0.71 wt%) of lipids was obtained, which was higher than that of the conventional Soxhlet extraction (CSE) (0.44 wt%). The composition of fatty acids in lipids was analyzed by gas chromatography with flame ionization and mass spectroscopic detection (GC-FID/MS), respectively. The major fatty acid compositions were palmitic acid (59.20–63.25%), oleic acid (1.85–9.43%), and arachidonic acid (9.44–15.12%), while the free fatty acid (FFA) content of the UAE (94.67%) was higher than that of the CSE (73.12%). Moreover, the proportions of unsaturated fatty acids in the UAE were monounsaturated fatty acids (MUFAs) (10.94%) and polyunsaturated fatty acids (18.20%), both of which were much higher than those in CSE. In addition, the enrichment of lipids by UAE displayed superior antioxidant activities in the DPPH (IC 50 = 18.65 mg/L), hydroxyl (IC 50 = 13.68 mg/L), and superoxide (IC 50 = 210.12 mg/L) radical scavenging assays. From the perspective of waste resource utilization and biorefinery, the green UAE technique could be potential for the enrichment of lipids from agar production wastes of G. lemaneiformis without compromising its quality, which may be useful in the algal development.
研究领域:
海洋生物学
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可持续发展
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食品科学
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 51
·29172-29178
·
IF 2.936
Jie Liu,Pinghe Yin,Ling Zhao
Macroalgae, the major contributor of primary productivity in coastal seas, contribute to the material cycle and energy flow in marine ecosystems. The purpose of this work was to evaluate the toxic effect of nano-TiO2 on the growth and antioxidant activity of Gracilaria lemaneiformis. An obvious inhibition of growth was observed in this study. The algae exposed to nano-TiO2 showed a negative growth rate at 20 mg L−1 and 40 mg L−1 during the 15 days exposure. The concentration of soluble protein increased slightly during the first 3 days of exposure, but it gradually diminished thereafter due to the high concentrations of nano-TiO2 and to prolonged exposure. Nano-TiO2 caused oxidative damage in G. lemaneiformis; superoxide anions accumulated, and nitrate reductase activity decreased linearly with the increase in nano-TiO2. Furthermore, extracts of G. lemaneiformis can scavenge DPPH· and hydroxyl radicals for their antioxidant capacity. However, the capacity to scavenge DPPH· and hydroxyl radicals in vitro decreased slightly with the increase in nano-TiO2. The results from this work imply that macroalgae can be an effective biomarker of nano-TiO2 contamination and can be useful indicators to evaluate the oxidative damage of increasing pollutants in marine ecosystems.
研究领域:
海洋生态学
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环境毒理学
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植物学
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DOI
PubMed
高影响因子
Food Chemistry-X
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2022
·
IF 6.443
Xiaoshan Long,Xiao Hu,Huan Xiang,Shengjun Chen,Laihao Li,Bo Qi,Chunsheng Li,Shucheng Liu,Xianqing Yang
This research aimed to analyze structural characterization and hypolipidemic activity in vitro of G. lemaneiformis polysaccharide (GLP) and its degradation products. The results presented that the content of galacturonic acid declined and glucuronic acid level enhanced, average particle size decreased from 99.9 μm to 25.7 μm, and color brightness of polysaccharide strengthened after degraded by H 2 O 2 -Vc. There was no significant change in thermal stability of polysaccharide before and after degradation. It was observed in AFM analysis, polysaccharide changed to smaller, delicacy and dispersion after degradation. As seen in FT-IR, H 2 O 2 -Vc degradation never change the structure of polysaccharide. Polysaccharide and its degradation products showed a significant inhibition effect on pancreatic lipase and cholesterol esterase in a dose-dependent manner, which presented the mixed type of competitive and non-competitive for pancreatic lipase, and non-competitive for cholesterol esterase, respectively. The fluorescence quenching type was static on pancreatic lipase and dynamic on cholesterol esterase.
研究领域:
海洋生物学
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生物化学
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药理学
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DOI
PubMed
高影响因子
Journal of Ocean University of China
·
2019
·Vol. 18
·期 5
·1130-1138
·
IF 0.699
Liu Jie,Yin Pinghe,Zhao Ling
Recently, the widespread use of TiO 2 nanoparticles (nano-TiO 2 ) has attracted the global concern regarding their adverse effects to aquatic organisms. Nanoparticles may get to the aquatic environments and harm the growth of algae. This paper presents the studies of nano-TiO 2 concentrations influence on the photosynthetic characteristics, antioxidant system and antitumor activity of marine macroalgae Gracilaria lemaneiformis after exposed to 0, 5, 10, 20 or 40 mg L -1 of nano-TiO 2 . Results showed that the photosynthetic pigment content of G. lemaneiformis obviously decreased and the antioxidant defense system was significantly disturbed after exposure to nano-TiO 2 . Compared with control, the maximum level of malondialdehyde showed 3.3-fold increase for 15 days exposure to nano-TiO 2 . The activity of superoxide dismutase and catalase enhanced obviously by 1.10–2.24-fold. The aqueous extract of G. lemaneiformis (AEGL) possesses anticancer activity against HepG2 liver cancer cells proliferation and the 48 h-cell inhibition exceeds 35%. But the toxicity of nano-TiO 2 would inhibit the anticancer activity of AEGL. When G. lemaneiformis was exposed to nano-TiO 2 , the effect of the aqueous extract of G. lemaneiformis on the cell inhibition, reactive oxygen species production, apoptosis, cycle arrest of HepG2 cells significantly lessened, and which were linearly associated with nano-TiO 2 exposure concentration. Those results further confirmed that nano-TiO 2 can inhibit the anticancer activity of G. lemaneiformis . The results in this work imply that macroalgae can be an effective biomarker of nano-TiO 2 contaminant, and can be useful indicator to evaluate ecological effects of increasing pollutants in marine ecosystem.
研究领域:
环境科学
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海洋生物学
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毒理学
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药理学
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DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 15
·9141-9151
·
IF 3.108
Lingrong Wen,Yunlin Zhang,Dongxiao Sun-Waterhouse,Lijun You,Xiong Fu
In the present study, polysaccharides from Gracilaria lemaneiformis (GLP) were obtained by citric acid extraction, and had a low molecular weight (21.2 kDa) with a high amount of galactose. GLP showed effective inhibitory effects on α-amylase activity. After a six-week animal trial, GLP was found to combat streptozotocin (STZ)-induced diabetes and associated with oxidative stress, via its regulation of glucose and lipid metabolism, repair of pancreatic β-cells, protection of liver and kidney function, promotion of the activities of endogenous antioxidant enzymes, and suppression of lipid peroxidation. A dose-dependent manner was found in these examinations except for the glucokinase level, superoxide dismutase activity, and malondialdehyde content. GLP treatments were also more effective at regulating the insulin level, lipidemic parameters and blood urea nitrogen level than metformin treatment. Thus, GLP is more advantageous than metformin not only in the naturalness of GLP but also in the action of combating STZ-induced diabetes. Accordingly, GLP may be utilized as a potential functional food supplement for diabetes prevention in the future.
研究领域:
药理学
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生物化学
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营养学
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DOI
高影响因子
Food Chemistry-X
·
2021
·
IF 5.182
Si-Yuan Lu,Yang Liu,Shijie Tang,Wancong Zhang,Qiuyong Yu,Changqi Shi,Kit-Leong Cheong
Gracilaria lemaneiformis polysaccharide (GLP) has varieties of antioxidation, however, the therapeutic effects of GLP on ulcerative colitis (UC) and the potential mechanisms involved are still incomplete. In the study, the analysis of the ζ-potential, thermal, and morphology properties demonstrated that GLP was a negatively charged polymer, and had great thermostability and irregular network. Moreover, the GLP treatment has the effects of reducing the severity of colitis caused by dextran sulfate sodium by alleviating the colon damage of mice, and increasing the amount of short-chain fatty acids in the intestines, alleviating histopathological inflammation. The sequencing results and α-diversity analysis showed that GLP could improve biodiversity, restore the abundance of Bacteroidetes, and decrease the proportion of Firmicutes. The level of CCL-25 and CCR-9 were inhibited, CD40 and TGF-β1 were increased. In summary, GLP has potentiality to be utilized as a hopeful functional food to the UC patients.
研究领域:
药理学
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免疫学
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微生物学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
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2019
·
IF 3.197
Rui Han,Daorui Pang,Lingrong Wen,Lijun You,Riming Huang,Viktoryia Kulikouskaya
The study explored the changes of a sulfated polysaccharide (SP) from Gracilaria Lemaneiformis during simulated human digestive system. The results showed SP was scarcely degraded without significant difference. Moreover, the results of fermentation in vitro indicated 53.7% of SP were utilized by gut microbiota with the molecular weight decreasing by 59.41% at 48 h. Meanwhile, 50.07 ± 2.82 mM of short chain fatty acids were produced in culture medium. Besides, in contrast with the regulation of galacto-oligosaccharide sacrificing diversity and richness of community, SP could regulate the composition of microbiota in a mild way. What’s more, Proteobacteria and Firmicutes were the most sensitive phyla responding to changes of pH values in this study. And Sutterella, Phascolarctobacterium, Parabacteroides, Lachnospiraceae_UCG-004, Desulfovibrio and Bacteroides were dominant genera for degrading and utilizing SP. Furthermore, the metagenomic function characteristics of microbes might be changed by SP. Thus, SP could be a potential prebiotic.
研究领域:
食品科学
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微生物学
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营养学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Yiyun Sun,Chenxi Zhang,Panpan Zhang,Chunqing Ai,Shuang Song
The health effects of polysaccharides have attracted lots of attention, but the exact mechanism remains unclear. This study indicated that polysaccharides from Gracilaria lemaneiformis (GLPs) tolerated the conditions of mouth, stomach, and small intestine, and it reached the colon integrally, where it increased the production of short chain fatty acids, altered the gut microbiota, and especially increased the level of Bacteroides . To explore the underlying mechanism, hundreds of Bacteroides strains were isolated from the human feces and identified by MALDI-TOF/MS. It showed that Bacteroides species profile was different between individuals, revealing an inherent difference in the human gut microbiota. The use of Bacteroides on GLPs was species-dependent, and various small molecular GLPs fragments can be liberated from growth of Bacteroides species. On the other hand, Bacteroides species that unable to grow with GLPs can live in GLPs-derived fragments, forming a GLPs utilization network. It should be noted that small molecular GLPs fragments can be easier to be metabolized by intestinal microbes and have better effect on cellular response. It suggested that the effect of polysaccharides cannot only be attributed to modulation of the gut microbiota, but also associated with the effect of microbial degradation on GLPs own activities.
研究领域:
营养学
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微生物学
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胃肠病学
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食品科学
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DOI
PubMed
高影响因子
Food & Function
·
2020
·Vol. 11
·期 12
·10876-10885
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IF 4.171
Xiong Li,Shiming Huang,Xi Chen,Qijun Xu,Yongxuan Ma,Lijun You,Viktoryia Kulikouskaya,Jianbo Xiao,Jinhua Piao
A sulfated polysaccharide extracted from Gracilaria lemaneiformis (GLP) with a prominent effect in regulating lipid metabolism was isolated. The molecular weight was 31.5 kDa and it was composed mainly of galactose, glucose and xylose. Fourier-transform infrared (FT-IR) spectrum and nuclear magnetic resonance (NMR) analysis suggested that GLP was composed of the following repeating unit: [3-β-Gal-4(OSO3)-1→4-α-3,6-anhydrogal-2(OSO3)-1→]. GLP could significantly decrease serum total cholesterol, triglyceride and free fatty acid levels and lower alanine aminotransferase and aspartate aminotransferase activities in high-fat-diet mice. Additionally, GLP could keep the body weight and attenuate accumulation of fat surrounding the liver and epididymis induced by high-fat diet. Results of RT-PCR indicated that GLP might regulate lipid metabolism and accelerate free fatty acid oxidation by up-regulating the expression of the PPARα, ACS and CPT1a gene. The present study suggests that GLP may be potentially useful for regulating lipid metabolism.
研究领域:
海洋生物学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2019
·Vol. 11
·期 1
·1049-1062
·
IF 3.241
Yuxin Xu,Chunqing Ai,Pingrui Jiang,Xiaona Sun,Yili Liu,Guoping Jiang,Shuang Song
Polysaccharides from Gracilaria lemaneiformis (GLPs) were shown to reduce high fat diet (HFD)-induced metabolic syndrome (MS), but its high molecular weight (MW) causes lots of barriers to its application in foods as a functional ingredient. In this study, oligosaccharides derived from GLPs (D-GLPs) were prepared, and its effect on HFD-induced MS and inflammation was evaluated in mice. It revealed that D-GLPs reduced fat accumulation, lipid disorders and inflammation in HFD-fed mice accompanied by an improvement of the gut microbiota. D-GLPs reversed HFD-induced gut microbiota dysbiosis characterized by the increased ratio of Bacteroidetes to Firmicutes and the abundance of the Bacteroidales. Spearman correlation analysis showed that some members of the Bacteroidales were positively correlated with reduced fat accumulation, inflammation and lipid disorders. Compared to GLPs, D-GLPs had a better inhibition effect on MS-related parameters, which may be attributed to higher abundance of some Bacteroidales members owing to their prioritization strategy to D-GLPs. The anti-obesity effect was transmissible through fecal transfer from D-GLPs-fed mice to HFD-fed mice, implying that the effect was associated with the gut microbiota. It indicated that D-GLPs can be used as a potential prebiotic agent to MS therapy.
研究领域:
海洋生物学
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营养学
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微生物学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
Marine Drugs
·
2025
·Vol. 23
·期 11
·423
·
IF 5.4
Pingting Liu,Fengyuan Li,Zhicong Liu,Yang Liu
Galectin-3 (Gal-3) is a histologic marker of pancreatic cancer and a potential therapeutic target. This study aimed to characterize a novel sulfated agarose-derived oligosaccharide (DP9) from marine algae,Gracilaria lemaneiformis, evaluate its Gal-3 inhibitory activity, and investigate its anti-pancreatic cancer mechanisms. Through controlled acid hydrolysis, a series of odd-numbered oligosaccharides (DP3-11) were obtained, in which DP9 showed the strongest Gal-3 inhibition in hemagglutination assays. Structural analysis confirmed DP9’s unique composition including an alternating β (1→4)-D-galactose and α (1→3)-3,6-anhydro-L-galactose backbone, featuring partial 6-O-methylation on β-D-galactose and 6-O-sulfation on 3,6-anhydro-α-L-galactose residues. Molecular docking revealed DP9’s binding to Gal-3’s carbohydrate recognition domain through key hydrogen bonds (His158, Arg162, Lys176, Asn179 and Arg186) and hydrophobic interactions (Pro117, Asn119, Trp181 and Gly235), with the sulfate group enhancing binding affinity. In vitro studies demonstrated DP9’s selective anti-pancreatic cancer activity against BxPC-3 cells, including inhibition of cell proliferation; S-phase cell cycle arrest; induction of apoptosis; and suppression of migration and invasion. Mechanistically, DP9 attenuated the Gal-3/EGFR/AKT/FOXO3 signaling pathway while showing minimal cytotoxicity to normal cells. This study first demonstrated that agarose-derived odd-numbered oligosaccharides (DP9) can serve as effective Gal-3 inhibitors, which proved its potential as a marine oligosaccharide-based therapeutic agent for pancreatic cancer.
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DOI
PubMed
高影响因子
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